##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937253_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1395083 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.569928814271265 31.0 31.0 34.0 30.0 34.0 2 31.665645699933265 33.0 31.0 34.0 30.0 34.0 3 31.72080370845319 33.0 31.0 34.0 30.0 34.0 4 35.352148940242266 37.0 35.0 37.0 33.0 37.0 5 35.324817233096525 37.0 35.0 37.0 33.0 37.0 6 35.38612971414604 37.0 35.0 37.0 33.0 37.0 7 35.3276858796215 37.0 35.0 37.0 33.0 37.0 8 35.3064082925532 37.0 35.0 37.0 33.0 37.0 9 36.910453356538646 39.0 37.0 39.0 33.0 39.0 10-11 36.8106177195192 39.0 37.0 39.0 33.0 39.0 12-13 36.69000124006959 39.0 37.0 39.0 32.0 39.0 14-15 37.84139366618331 40.0 38.0 41.0 33.0 41.0 16-17 37.773903058097616 40.0 38.0 41.0 33.0 41.0 18-19 37.70832954024958 40.0 37.5 41.0 32.0 41.0 20-21 37.62820742565138 40.0 37.0 41.0 32.0 41.0 22-23 37.491509465745054 40.0 37.0 41.0 32.0 41.0 24-25 37.347374313929706 40.0 37.0 41.0 32.0 41.0 26-27 37.185874962278234 39.0 36.5 41.0 31.0 41.0 28-29 36.98871930917372 39.0 36.0 41.0 31.0 41.0 30-31 37.00463269927309 39.0 36.0 41.0 31.0 41.0 32-33 36.8751547398972 39.0 36.0 41.0 30.5 41.0 34-35 36.684453183072264 39.0 36.0 41.0 30.0 41.0 36-37 36.71144906790492 39.0 36.0 41.0 30.0 41.0 38-39 36.699839005994626 39.0 36.0 41.0 30.0 41.0 40-41 36.55005938714757 39.0 35.5 41.0 30.0 41.0 42-43 36.32856503878264 39.0 35.0 41.0 30.0 41.0 44-45 36.135204141975784 39.0 35.0 41.0 29.5 41.0 46-47 35.85989758315455 39.0 35.0 41.0 28.5 41.0 48-49 35.58991508032139 39.0 35.0 40.0 27.0 41.0 50-51 34.68435032180881 38.0 34.0 39.5 25.5 40.5 52-53 34.826021104120684 38.0 34.5 39.5 26.0 40.5 54-55 35.18627063766098 39.0 35.0 40.0 26.0 41.0 56-57 34.96720589384287 38.0 34.5 40.0 26.0 41.0 58-59 34.728723667337356 38.0 34.0 40.0 26.0 41.0 60-61 34.44361267394126 38.0 34.0 40.0 24.5 41.0 62-63 34.11817003002689 37.5 34.0 40.0 23.5 41.0 64-65 33.759968403313636 37.0 33.0 40.0 23.0 41.0 66-67 33.36905725322436 36.5 33.0 39.0 22.0 41.0 68-69 32.88307505718298 36.0 32.0 39.0 20.5 40.5 70-71 32.45702442076923 35.5 32.0 39.0 20.0 40.0 72-73 32.03531653672219 35.0 31.0 38.0 18.5 40.0 74-75 31.56661180732616 35.0 31.0 37.0 17.5 39.0 76-77 31.123000925392972 35.0 31.0 37.0 13.5 39.0 78-79 30.642495106025947 34.5 30.0 36.0 8.0 39.0 80-81 30.17981044855396 34.0 30.0 36.0 7.0 37.5 82-83 29.734097899551497 34.0 29.5 35.0 4.0 37.0 84-85 29.253752285706298 34.0 29.0 35.0 2.0 36.5 86-87 28.806302922478448 34.0 29.0 35.0 2.0 36.0 88-89 28.422043706360125 34.0 28.5 35.0 2.0 36.0 90-91 27.972777605346778 34.0 27.0 35.0 2.0 35.0 92-93 27.501411027157523 33.0 26.5 35.0 2.0 35.0 94-95 26.950707592308127 33.0 25.5 35.0 2.0 35.0 96-97 26.30576030243362 33.0 25.0 35.0 2.0 35.0 98-99 25.586251857416368 32.0 23.0 34.5 2.0 35.0 100-101 23.73971226084756 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08378619362197881 1101 2 -0.07606842841624584 1101 3 0.08369378220820067 1101 4 0.06715964910302574 1101 5 0.11680762551028323 1101 6 -0.06393895485754086 1101 7 0.10906017648192545 1101 8 -0.06915426328179564 1101 9 -0.10871184417169388 1101 10-11 -0.005131556828949613 1101 12-13 0.08830661386452476 1101 14-15 0.02468332170980858 1101 16-17 0.013877585894846334 1101 18-19 0.1490013351983137 1101 20-21 0.18055605388553886 1101 22-23 0.2189527409338652 1101 24-25 0.27617456107988403 1101 26-27 0.3365208207347692 1101 28-29 0.16848951501513199 1101 30-31 0.09420924274374443 1101 32-33 0.23103070653451852 1101 34-35 9.875094167171028E-4 1101 36-37 0.029613302214833936 1101 38-39 0.13121990053588917 1101 40-41 0.11080874766003745 1101 42-43 0.23891718003441298 1101 44-45 0.10835124025338416 1101 46-47 0.2001015101961201 1101 48-49 0.17768486543271678 1101 50-51 0.1796736843821165 1101 52-53 0.12314148650373369 1101 54-55 0.26550788188826857 1101 56-57 0.19583080121082475 1101 58-59 0.23228073777980285 1101 60-61 0.23579071360792625 1101 62-63 0.4209026994488383 1101 64-65 0.21317193768226872 1101 66-67 0.3147376451906254 1101 68-69 0.14800499006305756 1101 70-71 0.18294708525397851 1101 72-73 0.3226321893386874 1101 74-75 0.11957681020568955 1101 76-77 0.1943454131573752 1101 78-79 0.2935583663072556 1101 80-81 0.3151195413853429 1101 82-83 0.35276167773298894 1101 84-85 0.388453238104983 1101 86-87 0.6364914057885862 1101 88-89 0.5820137407073069 1101 90-91 0.644987563455846 1101 92-93 0.7287691207071063 1101 94-95 0.6060560114412077 1101 96-97 0.42988561893950106 1101 98-99 0.3608723354528607 1101 100-101 0.7042121626875009 1102 1 -0.04129442787647619 1102 2 -0.003558453587622523 1102 3 0.09598156605566999 1102 4 -0.19765866173673885 1102 5 -0.0013338429987115319 1102 6 0.07686344981255644 1102 7 0.009824020740708761 1102 8 0.10831233108410032 1102 9 0.18983343753352955 1102 10-11 0.08775568744054851 1102 12-13 0.28154130350677775 1102 14-15 0.17564047011383366 1102 16-17 0.2812306077993014 1102 18-19 0.3560392797141603 1102 20-21 0.45573078074929185 1102 22-23 0.5137474201326668 1102 24-25 0.4697623864333522 1102 26-27 0.5638640686460761 1102 28-29 0.502156737326608 1102 30-31 0.4849455390697912 1102 32-33 0.2484950247650204 1102 34-35 0.22287305327918716 1102 36-37 0.3531542324847621 1102 38-39 0.4066364667848177 1102 40-41 0.39924028954091995 1102 42-43 0.37846101572421986 1102 44-45 0.09282833162013304 1102 46-47 0.0890110405088862 1102 48-49 0.16035109026133654 1102 50-51 0.10237294664647578 1102 52-53 0.03805167957357014 1102 54-55 0.2346001390112349 1102 56-57 0.19466093456558298 1102 58-59 0.21537039073609776 1102 60-61 0.2863585759151235 1102 62-63 0.29482488239656135 1102 64-65 0.24847546703102807 1102 66-67 0.22604535191727138 1102 68-69 0.2773229527879977 1102 70-71 0.13186709248405748 1102 72-73 -0.002584043523356172 1102 74-75 0.005001008202690116 1102 76-77 0.029994212161231104 1102 78-79 0.060021854417794174 1102 80-81 0.13893076273281935 1102 82-83 0.22409810393265062 1102 84-85 0.2223187854486035 1102 86-87 0.2405994954415398 1102 88-89 0.3598813365727729 1102 90-91 0.28283936026389966 1102 92-93 0.030018986816788384 1102 94-95 -0.0055608145549364 1102 96-97 0.14459631543692808 1102 98-99 0.4184133728350403 1102 100-101 0.7873931957851887 1103 1 -0.07132203890911981 1103 2 -0.11564428809798244 1103 3 -0.03706388670641303 1103 4 -0.06230820269718862 1103 5 -0.26598534566663545 1103 6 -0.11265465674739517 1103 7 -0.08419816617077913 1103 8 -0.04435142244697232 1103 9 0.03705320762141895 1103 10-11 0.021548673355482606 1103 12-13 -0.052196018958703405 1103 14-15 -0.07149359787894127 1103 16-17 0.014483143306208035 1103 18-19 -0.09553351925005416 1103 20-21 -0.13268092279747634 1103 22-23 -0.15301120157166537 1103 24-25 -0.19082114797499372 1103 26-27 -0.1486446364308236 1103 28-29 -0.32846514846277586 1103 30-31 -0.28908721654069325 1103 32-33 -0.2001536040014571 1103 34-35 -0.19840669111032838 1103 36-37 -0.2351851330990442 1103 38-39 -0.22455455938435875 1103 40-41 -0.13511533859780656 1103 42-43 -0.04244048694370406 1103 44-45 -0.26187167426498803 1103 46-47 -0.24983591688282303 1103 48-49 -0.18149237529108575 1103 50-51 -0.4581745413871161 1103 52-53 -0.30462612468726036 1103 54-55 -0.22596892780453004 1103 56-57 -0.3874402012630398 1103 58-59 -0.384117603614186 1103 60-61 -0.2821117581636585 1103 62-63 -0.35548604823745933 1103 64-65 -0.25400854505308956 1103 66-67 -0.16561199663890847 1103 68-69 -0.15073633001175324 1103 70-71 -0.2457573042908976 1103 72-73 -0.1354284212697472 1103 74-75 -0.3297147158635383 1103 76-77 -0.16813395109206297 1103 78-79 4.11694734804513E-4 1103 80-81 0.10639890950777087 1103 82-83 0.05383538225970241 1103 84-85 0.13582465095393914 1103 86-87 -0.04160561088300696 1103 88-89 0.12954132362550297 1103 90-91 0.41787226790671284 1103 92-93 0.3417907029184839 1103 94-95 0.2587994971603784 1103 96-97 0.5380639521679385 1103 98-99 0.5201732696492272 1103 100-101 0.5189690844386696 1104 1 0.09927923842515085 1104 2 -0.01592335633063513 1104 3 0.050317389480785124 1104 4 -0.019387319955342264 1104 5 0.036129978966243925 1104 6 0.04609093468948089 1104 7 -0.011718206522537855 1104 8 0.04997199029352828 1104 9 0.12490038319477748 1104 10-11 -0.05076177905403512 1104 12-13 0.06725040929004678 1104 14-15 0.04819560087624808 1104 16-17 0.09033321326967325 1104 18-19 0.12699157685642604 1104 20-21 0.08142989683189228 1104 22-23 0.13353990161993323 1104 24-25 -0.008485569996558695 1104 26-27 0.0834429856517076 1104 28-29 -0.03725328366316916 1104 30-31 -0.15955108105431748 1104 32-33 -0.09927774458207494 1104 34-35 -0.09410886748113967 1104 36-37 -0.10231011748394536 1104 38-39 -0.15891381416532369 1104 40-41 -0.18816796165848615 1104 42-43 -0.061247148126433615 1104 44-45 0.10041675595263655 1104 46-47 0.01567311158732565 1104 48-49 -0.17722012505990392 1104 50-51 -0.18525669131243916 1104 52-53 0.0055668189404869395 1104 54-55 -0.013781219848802095 1104 56-57 0.12068907521013728 1104 58-59 -0.06390448410745364 1104 60-61 -0.031769797442201764 1104 62-63 -0.08574523128720557 1104 64-65 -0.04482247049504906 1104 66-67 -0.08905718510817451 1104 68-69 -0.0035457111669501273 1104 70-71 -0.02117455136763624 1104 72-73 -0.06437189031100488 1104 74-75 -0.04545716542580536 1104 76-77 0.1401722178714948 1104 78-79 0.07340528292217385 1104 80-81 0.020635514114751174 1104 82-83 0.10821676136902525 1104 84-85 0.0031173748311594807 1104 86-87 -0.02499861547835991 1104 88-89 0.002858723671231189 1104 90-91 0.11488446221425264 1104 92-93 0.1563275887149942 1104 94-95 0.18392286621790177 1104 96-97 0.1743344157967499 1104 98-99 0.3433789874053339 1104 100-101 0.37526426509056776 1105 1 -0.008386407250235095 1105 2 0.06822050805916291 1105 3 0.14486275807024995 1105 4 0.0289633768649864 1105 5 0.06449255279320454 1105 6 -0.02434958448168345 1105 7 0.07801125152268185 1105 8 0.0069116207627004655 1105 9 0.1368673797137845 1105 10-11 0.30489879235273776 1105 12-13 0.19630855934673264 1105 14-15 0.36871574243822636 1105 16-17 0.2120965838181803 1105 18-19 0.2774302049958024 1105 20-21 0.31728282266446683 1105 22-23 0.17451329364160983 1105 24-25 0.3280961080619136 1105 26-27 0.23013790997311645 1105 28-29 0.22011655653635387 1105 30-31 0.24790489232526625 1105 32-33 0.2123356787577393 1105 34-35 0.19601273083883086 1105 36-37 0.12954924780947863 1105 38-39 0.277686857150961 1105 40-41 0.31841146043224455 1105 42-43 0.2648432431927361 1105 44-45 0.29893673329343073 1105 46-47 0.2618528956281736 1105 48-49 0.14296452424078865 1105 50-51 0.13144655189001497 1105 52-53 -0.005861274990714094 1105 54-55 0.33123711403608525 1105 56-57 0.4314187729519361 1105 58-59 0.36759247489779057 1105 60-61 0.3061053297670142 1105 62-63 0.3764756030725138 1105 64-65 0.34294769615627985 1105 66-67 0.3752620310093917 1105 68-69 0.48893349785095097 1105 70-71 0.2707182304479403 1105 72-73 0.4105543138325096 1105 74-75 0.4345053622865187 1105 76-77 0.47612666225235145 1105 78-79 0.5630259651699454 1105 80-81 0.46069872268145673 1105 82-83 0.3099359414906324 1105 84-85 0.4783114751437658 1105 86-87 0.46015844383451565 1105 88-89 0.3846584551115413 1105 90-91 0.5647455792002809 1105 92-93 0.46009219341055996 1105 94-95 0.7004615150093443 1105 96-97 0.778838389036995 1105 98-99 0.7324264299092604 1105 100-101 1.024109616013817 1106 1 -0.15463679607375624 1106 2 -0.006058985050209742 1106 3 -0.1685114186382073 1106 4 -0.12166482670271961 1106 5 -0.07523371544768764 1106 6 -0.22926431379288914 1106 7 0.002845916999213216 1106 8 -0.07613973099072524 1106 9 -0.07542485328279724 1106 10-11 -0.08376987590979468 1106 12-13 -0.2342604078027506 1106 14-15 -0.21440390701201295 1106 16-17 -0.26288211541498896 1106 18-19 -0.10644477640828143 1106 20-21 -0.2613663706112561 1106 22-23 -0.19950991405101348 1106 24-25 -0.29902348729113726 1106 26-27 -0.31527735354780617 1106 28-29 0.0326427598606287 1106 30-31 0.02698821551537378 1106 32-33 0.022083991601761 1106 34-35 -0.014482123166388305 1106 36-37 -0.03941857788938563 1106 38-39 -0.18250708125939497 1106 40-41 -0.14413502933663125 1106 42-43 0.05715468069019636 1106 44-45 -0.1785710093662587 1106 46-47 0.14970672503270066 1106 48-49 0.012150091111671202 1106 50-51 0.056166260847149374 1106 52-53 -0.039410320851601455 1106 54-55 0.08514090219150461 1106 56-57 -0.14306493703305279 1106 58-59 -0.09502501742716163 1106 60-61 -0.04118845852365638 1106 62-63 0.20776068171628737 1106 64-65 0.12430986328953253 1106 66-67 0.06661189103292742 1106 68-69 0.10295007863834371 1106 70-71 0.2904143395078691 1106 72-73 0.4633545194018147 1106 74-75 0.35063105709724596 1106 76-77 0.343388397960549 1106 78-79 0.35263186717289585 1106 80-81 0.31512522489268235 1106 82-83 0.1623554381163217 1106 84-85 0.3273411068771743 1106 86-87 0.16530984833269358 1106 88-89 0.5608299972870583 1106 90-91 0.44555460261083724 1106 92-93 0.350092328653524 1106 94-95 0.43673164930561015 1106 96-97 0.4594918830389716 1106 98-99 0.5925710046377581 1106 100-101 0.9725414738464124 1107 1 0.037751857500882835 1107 2 -0.02479545816905926 1107 3 0.016732386105552166 1107 4 0.0065396886753248396 1107 5 0.13704703289588593 1107 6 0.12211105743563166 1107 7 0.12541970904042188 1107 8 0.07421545337319202 1107 9 0.10780557616940456 1107 10-11 0.06506432270133189 1107 12-13 0.13420320405708708 1107 14-15 0.0738223398953437 1107 16-17 0.009255939546747527 1107 18-19 0.01314236879462527 1107 20-21 0.17462564493276744 1107 22-23 0.13932645328707594 1107 24-25 0.05220472032242185 1107 26-27 0.1303980278604584 1107 28-29 0.08513701716015731 1107 30-31 0.07084385796753878 1107 32-33 -0.011497638838314117 1107 34-35 0.07688236016760897 1107 36-37 -0.02877256281451679 1107 38-39 0.02273769719850094 1107 40-41 0.14947452899549063 1107 42-43 -0.10535909515194675 1107 44-45 -0.010078186082090212 1107 46-47 -0.026071693035895294 1107 48-49 -0.17385608754717197 1107 50-51 -0.12842471282272783 1107 52-53 -0.16188652017974192 1107 54-55 -0.0766200155783423 1107 56-57 -0.024001384481259436 1107 58-59 -0.1508360991793225 1107 60-61 -0.08752508035520634 1107 62-63 0.09030884737162381 1107 64-65 0.05299946535026123 1107 66-67 0.07898956819357039 1107 68-69 0.17251752825969646 1107 70-71 0.2753277453386431 1107 72-73 0.20045878205678136 1107 74-75 0.09512072023641238 1107 76-77 0.0068559710665674345 1107 78-79 0.19331342281402186 1107 80-81 0.22010024027293795 1107 82-83 0.1168177044802512 1107 84-85 0.06818753002678335 1107 86-87 0.2529777586978845 1107 88-89 0.48140501974887684 1107 90-91 0.610946146346091 1107 92-93 0.627636267504279 1107 94-95 0.5107916832085628 1107 96-97 0.44430061459925696 1107 98-99 0.5313595714633195 1107 100-101 0.8700890419953389 1108 1 -0.2081106841523166 1108 2 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-1.4118904706717998 2316 62-63 -1.2899557182765378 2316 64-65 -1.5199126018159106 2316 66-67 -1.2626977432685607 2316 68-69 -1.3094925103375488 2316 70-71 -1.3361858528078656 2316 72-73 -1.5895012497715264 2316 74-75 -1.768973501143961 2316 76-77 -1.6901987682492177 2316 78-79 -1.63361188405894 2316 80-81 -1.4736437155193052 2316 82-83 -1.2365518860073585 2316 84-85 -1.6800546872613786 2316 86-87 -1.7520081236322085 2316 88-89 -1.5689287718922529 2316 90-91 -1.782263091631041 2316 92-93 -1.7812109389221646 2316 94-95 -1.521903446523769 2316 96-97 -1.665997715785636 2316 98-99 -2.155360108105846 2316 100-101 -2.5177830141577466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5739.0 3 1195.0 4 1123.0 5 1270.0 6 1455.0 7 1652.0 8 1969.0 9 2615.0 10 3222.0 11 3956.0 12 4805.0 13 6061.0 14 6902.0 15 8039.0 16 9931.0 17 12179.0 18 10852.0 19 8265.0 20 8687.0 21 9279.0 22 10036.0 23 10985.0 24 12511.0 25 14302.0 26 16755.0 27 19856.0 28 23227.0 29 28496.0 30 34523.0 31 42813.0 32 54361.0 33 70357.0 34 95423.0 35 133519.0 36 194109.0 37 272033.0 38 223447.0 39 29130.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35860014710016 19.132439756155957 14.554432452913208 22.954527643830673 2 15.197437744110792 20.05090298532137 40.62758306415559 24.12407620641225 3 19.23364437614377 24.46353381242839 29.48749096126173 26.815330850166113 4 12.620574155801217 16.434023834509727 37.5978609318172 33.34754107787185 5 13.717205618659872 37.204037558348034 34.420580192391306 14.658176630600783 6 31.80410079388508 36.27888903493368 17.696964734494173 14.220045436687073 7 28.71949252922978 32.34652076379207 20.675152766314767 18.258833940663386 8 26.274306146651067 35.29341202954484 19.639359311128015 18.79292251267608 9 26.059425056437785 17.058612697083337 20.347267463032622 36.534694783446255 10-11 24.859637125333848 26.94205757320204 27.412531804330992 20.78577349713312 12-13 25.673184294335915 23.974200064435188 28.130377455873212 22.222238185355685 14-15 22.47188590246118 25.576062931048416 26.260892361117342 25.691158805373053 16-17 22.08813172170934 28.998672942207442 26.81317576242972 22.100019573653505 18-19 22.027064975620405 27.513281473879108 29.010674735501702 21.44897881499878 20-21 22.61822890873551 26.657657518538723 29.178399448102887 21.54571412462288 22-23 22.262227709509887 26.598944630825482 29.044594267508145 22.094233392156482 24-25 22.15672396686736 26.868396984063885 29.069842182959178 21.905036866109572 26-27 22.07816554287613 27.05763204912955 28.975049156483717 21.889153251510606 28-29 21.833907760531197 27.185974710838124 29.093992378559552 21.886125150071127 30-31 22.079146473124723 26.86694637730192 29.16769923995423 21.88620790961913 32-33 21.83889055700578 27.027552182926495 29.059773496863063 22.073783763204663 34-35 22.276091141902583 27.010163628639162 28.85459952588168 21.859145703576573 36-37 22.172524512382868 26.881473296289286 28.891950156089912 22.05405203523793 38-39 21.978264265310262 27.07932124755145 28.99363153387209 21.948782953266196 40-41 22.274078587949205 26.96797541249391 28.89089419853068 21.867051801026204 42-43 22.08737611327935 26.916197799348847 28.814253135567018 22.182172951804784 44-45 22.163534471860526 27.10220024594026 28.579637525427238 22.154627756771976 46-47 22.150837116153436 27.319220939556633 28.250259719133464 22.27968222515647 48-49 22.231048883944045 27.3274524302988 28.14713486440799 22.294363821349165 50-51 22.268781103104278 27.407796894574126 28.146262168281446 22.177159834040154 52-53 22.44097655728072 27.522495212179816 27.845753889520243 22.190774341019228 54-55 22.21530214918552 27.369942435288635 28.024571766604854 22.39018364892099 56-57 22.210484587951584 27.67935240814603 27.975550132784484 22.134612871117902 58-59 22.387103167336985 27.40142508263196 27.990709604407805 22.22076214562325 60-61 22.331896595059554 27.33988219799412 28.349393108539566 21.97882809840676 62-63 22.310647965124733 27.43271031963608 28.23435885890576 22.022282856333423 64-65 22.04861840444627 27.450264391253814 28.130891892755567 22.37022531154435 66-67 22.06706007023234 27.674335678122002 27.912848410112623 22.345755841533034 68-69 22.030702036281873 27.487632974473858 28.105616211261147 22.376048777983122 70-71 22.07973054494269 27.47403837867341 28.0437218120709 22.402509264313007 72-73 22.203939099244558 27.33028417766886 28.05807377443622 22.40770294865036 74-75 21.961486560728616 27.649127544348122 27.89222441899129 22.497161475931975 76-77 22.101035521445162 27.52039369637632 27.797121684183914 22.581449097994604 78-79 22.16305817021723 27.550629793114144 27.8753298352009 22.41098220146772 80-81 22.05344396218665 27.793883624267878 27.788246378518433 22.364426035027044 82-83 22.139717714639904 27.7341433993541 27.80998833237749 22.316150553628507 84-85 22.24081732602487 27.482817382037585 27.740224524807527 22.536140767130018 86-87 22.147899941759803 27.426034589363518 27.929406560388482 22.496658908488204 88-89 22.15762253024677 27.352637114551882 27.883690391163885 22.60604996403747 90-91 22.209287545054927 27.481261674423884 27.85240152908471 22.45704925143648 92-93 22.27620712823402 27.35458279782341 27.88971565994393 22.479494413998633 94-95 22.088112431022452 27.243882198361597 27.9002349554705 22.767770415145453 96-97 22.03297750906736 27.357055988371947 27.925675828522124 22.684290674038568 98-99 21.984449280977643 27.279076623541243 27.772070253657056 22.96440384182406 100-101 21.92909323182252 27.22832249359274 28.105986021438202 22.736598253146543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1817.0 1 1031.0 2 253.5 3 245.5 4 207.0 5 203.5 6 321.0 7 625.0 8 1054.5 9 1336.5 10 1264.5 11 1164.5 12 1247.5 13 1357.0 14 1467.0 15 1574.5 16 1680.0 17 1755.0 18 1837.0 19 1985.0 20 2198.5 21 2495.5 22 3012.0 23 3630.5 24 4365.5 25 5505.0 26 7044.0 27 9115.0 28 11284.0 29 13854.0 30 16801.5 31 19088.0 32 21537.0 33 24418.0 34 27952.0 35 31173.0 36 34242.0 37 37696.0 38 40162.5 39 41825.5 40 43292.0 41 45979.5 42 48654.0 43 50498.5 44 52666.0 45 54284.5 46 54414.0 47 54841.0 48 57099.0 49 57063.5 50 54516.5 51 51716.5 52 48558.0 53 46331.0 54 44234.5 55 40566.5 56 36138.5 57 32023.5 58 28470.5 59 25082.0 60 21188.5 61 16976.5 62 13082.5 63 9900.5 64 7388.0 65 5665.5 66 4282.5 67 3261.5 68 2336.5 69 1527.0 70 1099.5 71 804.0 72 628.0 73 470.5 74 337.0 75 264.5 76 173.0 77 103.5 78 65.0 79 43.5 80 33.5 81 20.5 82 11.5 83 11.5 84 11.0 85 7.0 86 3.5 87 6.0 88 6.0 89 3.0 90 3.5 91 4.0 92 3.5 93 4.0 94 4.5 95 3.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.300627274506248 2 0.2730303501655457 3 0.2758975630840602 4 0.2740338746870258 5 0.26148981816852473 6 0.2018517894634226 7 0.2095932643434118 8 0.20744285465452592 9 0.20371547786045704 10-11 0.21339232146044357 12-13 0.21407328452859078 14-15 0.198231933153798 16-17 0.20826717836859884 18-19 0.19568728168861638 20-21 0.20031066251972104 22-23 0.19884121589898238 24-25 0.1920674253789918 26-27 0.20396635899082707 28-29 0.20260443285453267 30-31 0.210023346281189 32-33 0.21224543629303777 34-35 0.2017442689789783 36-37 0.1990204167063895 38-39 0.19178070408714035 40-41 0.1912789418264003 42-43 0.18862676987677435 44-45 0.20608092851823157 46-47 0.19450455635972913 48-49 0.2033212360841613 50-51 0.21066846918785476 52-53 0.21568609179525522 54-55 0.21486176808118226 56-57 0.18644052002640701 58-59 0.17647695513456904 60-61 0.18472019227529832 62-63 0.18475603243677974 64-65 0.18239058177900527 66-67 0.19385943345306336 68-69 0.18418258985307684 70-71 0.18945109359084727 72-73 0.19962969945157388 74-75 0.18880597068418153 76-77 0.19647576524120788 78-79 0.1950421587819506 80-81 0.18342994646196678 82-83 0.19844697412268658 84-85 0.1822113809715981 86-87 0.1845051513064097 88-89 0.19077717956566026 90-91 0.18751572487085 92-93 0.19486295797454345 94-95 0.19292758925454614 96-97 0.18554451598937124 98-99 0.19149398279528887 100-101 0.18077777451234084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1395083.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8358298812515 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93859311168008 56.10105529683839 2 9.741151199839166 13.021158488799989 3 2.726812080090661 5.4674624490924275 4 1.152286261098289 3.0805603408507434 5 0.6137705576625848 2.051093228902869 6 0.37435006802597487 1.5011998477571387 7 0.26960193761794 1.261334846680189 8 0.17786390197564006 0.9510145195567578 9 0.14408619203269055 0.8667108197040817 >10 0.7844008174413535 9.681333618881025 >50 0.0552244654990869 2.515477814699289 >100 0.02089504908303537 2.3594797998547916 >500 5.357544186541365E-4 0.26662332395082694 >1k 4.286035349233092E-4 0.8754956044316033 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4588 0.3288693217536161 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.24464494227225192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 0.16070728408273915 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1982 0.14207040011239475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.168032296286314E-5 0.0 10-11 0.0 0.0 0.0 7.168032296286314E-5 0.0 12-13 0.0 0.0 0.0 7.168032296286314E-5 0.0 14-15 0.0 0.0 0.0 7.168032296286314E-5 0.0 16-17 0.0 0.0 0.0 7.168032296286314E-5 0.0 18-19 0.0 0.0 0.0 7.168032296286314E-5 0.0 20-21 0.0 0.0 0.0 1.0752048444429471E-4 0.0 22-23 0.0 0.0 0.0 1.4336064592572627E-4 0.0 24-25 0.0 0.0 0.0 3.584016148143157E-4 0.0 26-27 0.0 0.0 0.0 8.601638755543577E-4 0.0 28-29 0.0 0.0 0.0 0.0012544056518501048 0.0 30-31 0.0 0.0 0.0 0.0021145695274044627 0.0 32-33 0.0 0.0 0.0 0.0036915366325874516 0.0 34-35 0.0 0.0 0.0 0.005662745514066188 0.0 36-37 0.0 0.0 0.0 0.007884835525914945 0.0 38-39 0.0 0.0 0.0 0.009999405053319407 0.0 40-41 0.0 0.0 0.0 0.01300997861775966 0.0 42-43 0.0 0.0 0.0 0.017705039771827195 0.0 44-45 0.0 0.0 0.0 0.026844280949592247 0.0 46-47 0.0 0.0 0.0 0.04390419781475367 0.0 48-49 0.0 0.0 0.0 0.07945763800433378 0.0 50-51 0.0 0.0 0.0 0.10748464428281328 0.0 52-53 0.0 0.0 0.0 0.13332540071092544 0.0 54-55 0.0 0.0 0.0 0.1635744970012537 0.0 56-57 0.0 0.0 0.0 0.18715732325603565 0.0 58-59 0.0 0.0 0.0 0.22127715698635853 0.0 60-61 0.0 0.0 0.0 0.2809868660144235 0.0 62-63 0.0 0.0 0.0 0.33256085838620353 0.0 64-65 0.0 0.0 0.0 0.3908369609550113 0.0 66-67 0.0 0.0 0.0 0.48441562258302906 0.0 68-69 0.0 0.0 0.0 0.5875277671651078 0.0 70-71 0.0 0.0 0.0 0.6906399117471864 0.0 72-73 0.0 0.0 0.0 0.7978736748996296 0.0 74-75 0.0 0.0 0.0 0.8999822949602281 0.0 76-77 0.0 0.0 0.0 0.9942060794949117 0.0 78-79 0.0 0.0 0.0 1.1049880186340166 0.0 80-81 0.0 0.0 0.0 1.283722903941916 0.0 82-83 0.0 0.0 0.0 1.4700559034838787 0.0 84-85 0.0 0.0 0.0 1.6665675088865681 0.0 86-87 0.0 0.0 0.0 1.9515684729869118 0.0 88-89 0.0 0.0 0.0 2.320399574792324 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3545 0.0 34.86405 1 GTATCAA 5705 0.0 33.82916 1 ATCAACG 6720 0.0 28.286982 3 TCAACGC 6815 0.0 27.755198 4 TATCAAC 6875 0.0 27.580112 2 CAACGCA 6875 0.0 27.51198 5 AACGCAG 7130 0.0 26.581306 6 GTACATG 5270 0.0 24.895409 1 TACATGG 5440 0.0 23.149565 2 ACGCAGA 8165 0.0 22.921795 7 CGCAGAG 8195 0.0 22.664804 8 ACATGGG 5405 0.0 22.244398 3 GTCCTAC 290 1.05501385E-10 21.30915 1 GAGTACT 4120 0.0 21.27197 12-13 GTACCTG 1280 0.0 21.168285 1 TACCTGG 1245 0.0 20.99371 2 GCAGAGT 8605 0.0 20.922447 9 GTATAGC 610 0.0 20.261158 1 AGAGTAC 7090 0.0 20.099422 10-11 AGTACTT 4445 0.0 19.716652 12-13 >>END_MODULE