FastQCFastQC Report
Thu 26 May 2016
SRR937253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395083
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52930.3794039494424346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44620.31983760106029535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29910.21439584598192366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.036.4631461
GTATCAA64050.033.31941
GCGTTCG605.3871718E-627.70708834-35
ATCAACG76550.027.616383
TCAACGC77050.027.437174
CAACGCA78050.027.1465025
TATCAAC78600.026.8356632
AACGCAG79950.026.4372126
GTACATG50050.025.6406881
TACATGG51300.024.5403352
ACATGGG49350.023.9697843
TACCTGG10800.023.7531912
CGTTCGG701.781282E-523.74893434-35
GTACCTG11050.023.6575831
ACGCAGA90300.023.1974647
CGCAGAG91900.022.7419058
GAGTACT51850.022.49015412-13
GTACTTT54550.021.2456114-15
GCAGAGT97250.020.7581679
CATGGGG26600.020.3598774
Produced by FastQC (version 0.11.5)