##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937253_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1395083 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11753709277512 33.0 31.0 34.0 31.0 34.0 2 32.35084435836434 34.0 31.0 34.0 31.0 34.0 3 32.437398348342 34.0 31.0 34.0 31.0 34.0 4 36.04962428758719 37.0 35.0 37.0 35.0 37.0 5 35.942587645322895 37.0 35.0 37.0 35.0 37.0 6 36.0164463333006 37.0 35.0 37.0 35.0 37.0 7 35.97453126444807 37.0 35.0 37.0 35.0 37.0 8 35.952044430331384 37.0 35.0 37.0 35.0 37.0 9 37.645401026318865 39.0 37.0 39.0 35.0 39.0 10-11 37.56793645969451 39.0 37.0 39.0 35.0 39.0 12-13 37.48873902126253 39.0 37.0 39.0 35.0 39.0 14-15 38.710407911213885 40.0 38.0 41.0 34.5 41.0 16-17 38.68086845012089 40.0 38.0 41.0 34.5 41.0 18-19 38.66371534883588 40.0 38.0 41.0 34.0 41.0 20-21 38.55335524839741 40.0 38.0 41.0 34.0 41.0 22-23 38.42992173225535 40.0 38.0 41.0 34.0 41.0 24-25 38.287945950169274 40.0 38.0 41.0 34.0 41.0 26-27 38.25115960842473 40.0 38.0 41.0 34.0 41.0 28-29 38.15938442372246 40.0 38.0 41.0 34.0 41.0 30-31 38.267477992348844 40.0 38.0 41.0 34.0 41.0 32-33 38.21503666806921 40.0 38.0 41.0 34.0 41.0 34-35 38.11641672932721 40.0 38.0 41.0 33.5 41.0 36-37 37.96700052971759 40.0 37.0 41.0 33.0 41.0 38-39 37.850307114343735 40.0 37.0 41.0 33.0 41.0 40-41 37.69789575243911 40.0 37.0 41.0 33.0 41.0 42-43 37.51399773346819 40.0 37.0 41.0 33.0 41.0 44-45 37.25676608488527 40.0 37.0 41.0 32.0 41.0 46-47 36.943590453041146 40.0 36.0 41.0 31.0 41.0 48-49 36.679547381768685 39.0 36.0 41.0 31.0 41.0 50-51 36.4784464436883 39.0 36.0 41.0 30.0 41.0 52-53 36.25543426448462 39.0 35.5 40.5 30.0 41.0 54-55 36.02613106173611 39.0 35.0 40.0 29.5 41.0 56-57 35.784968349553395 38.5 35.0 40.0 29.0 41.0 58-59 35.53456891095368 38.0 35.0 40.0 28.0 41.0 60-61 35.21052259973062 38.0 34.0 40.0 28.0 41.0 62-63 34.838888797297365 37.5 34.0 40.0 27.5 41.0 64-65 34.492867807865196 37.0 34.0 40.0 26.5 41.0 66-67 34.056783718244716 36.5 33.5 39.0 26.0 41.0 68-69 33.65961236714948 36.0 33.0 39.0 26.0 40.0 70-71 33.25665390517983 35.5 32.5 38.5 25.5 40.0 72-73 32.80106524127955 35.0 32.0 38.0 24.5 40.0 74-75 32.346097687377735 35.0 32.0 37.0 23.5 39.0 76-77 31.021401594027022 34.0 30.5 36.0 21.5 38.5 78-79 31.66974581440674 34.5 31.0 36.0 23.0 38.5 80-81 31.53187301400705 35.0 31.0 36.0 23.0 37.5 82-83 31.19484790510672 34.0 31.0 35.5 21.5 37.0 84-85 30.81753343707865 34.0 31.0 35.0 20.0 36.5 86-87 30.43960932790379 34.0 31.0 35.0 19.0 36.0 88-89 30.032294852707686 34.0 30.0 35.0 17.5 36.0 90-91 29.57304475791046 34.0 30.0 35.0 10.5 35.0 92-93 29.189333537861188 34.0 29.0 35.0 4.5 35.0 94-95 28.751889314112496 34.0 29.0 35.0 2.0 35.0 96-97 28.24771070968537 33.5 29.0 35.0 2.0 35.0 98-99 27.70595943037081 33.0 28.5 35.0 2.0 35.0 100-101 25.709113006179564 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07899745414491832 1101 2 0.07301819720954938 1101 3 0.05448950957729437 1101 4 0.0912704105693436 1101 5 -0.06491419799456821 1101 6 -0.012187412237892659 1101 7 -0.10065194415658141 1101 8 0.02860246853804682 1101 9 0.039318554475855194 1101 10-11 -0.059329535265753464 1101 12-13 0.037758035366259435 1101 14-15 0.015868569863279447 1101 16-17 -0.040299011811193 1101 18-19 -0.11733669036834726 1101 20-21 0.01923217000558708 1101 22-23 -0.0458357021720488 1101 24-25 -0.1712446741930549 1101 26-27 -0.12956917593967887 1101 28-29 -0.15654407624881372 1101 30-31 -0.06502825714712657 1101 32-33 -0.10598035261794791 1101 34-35 -0.13759881306962285 1101 36-37 -0.1882918393261619 1101 38-39 -0.08786732270682052 1101 40-41 -0.03498562819376616 1101 42-43 -0.06033093856670746 1101 44-45 -0.08923657378265659 1101 46-47 -0.053385092324894856 1101 48-49 -5.859429101917613E-4 1101 50-51 -0.18405142102102445 1101 52-53 -0.002900724030169499 1101 54-55 -0.06758278865410716 1101 56-57 0.07134965031610108 1101 58-59 -0.10027263838857436 1101 60-61 -0.07739207938858783 1101 62-63 -0.1874299333315932 1101 64-65 -0.23420130628104374 1101 66-67 -0.24825708845124694 1101 68-69 -0.12086560151281844 1101 70-71 -0.20190651080049804 1101 72-73 -0.17728589049630727 1101 74-75 -0.25834617896270373 1101 76-77 -0.2955911974161758 1101 78-79 -0.2275471105567597 1101 80-81 -0.0555177660309738 1101 82-83 -0.2607958119027245 1101 84-85 -0.138787324770135 1101 86-87 -0.1456049915431592 1101 88-89 -0.12686965691885987 1101 90-91 -0.07041708224369714 1101 92-93 -0.17414828784328407 1101 94-95 -0.3243529619522256 1101 96-97 -0.42066331980675997 1101 98-99 -0.04493036245963111 1101 100-101 0.34271964603951943 1102 1 0.13931671560592918 1102 2 0.0718131843560812 1102 3 0.11996856913170717 1102 4 0.09431306802435557 1102 5 -0.004722132334755713 1102 6 0.10292144058987418 1102 7 0.006256126751729596 1102 8 0.11411319315244128 1102 9 0.2738910426935064 1102 10-11 0.10469949215498531 1102 12-13 0.0894037147257194 1102 14-15 0.13477823406636702 1102 16-17 0.07537385397715468 1102 18-19 0.04006643998726389 1102 20-21 0.11962396654585206 1102 22-23 0.2090889510520384 1102 24-25 0.14537412668526173 1102 26-27 0.119854258882512 1102 28-29 0.18220177315136254 1102 30-31 0.19548213274147486 1102 32-33 0.22168439941718532 1102 34-35 0.2038332108164056 1102 36-37 0.12293536584736131 1102 38-39 0.02199972420834939 1102 40-41 0.03402897254530757 1102 42-43 0.005413391178365146 1102 44-45 0.14132422581032245 1102 46-47 0.1940977697145243 1102 48-49 0.2876297008344437 1102 50-51 0.25179587037785467 1102 52-53 0.10612699795998282 1102 54-55 0.15754959539688684 1102 56-57 0.15378340808173618 1102 58-59 0.22147165118939682 1102 60-61 0.18421286384192115 1102 62-63 0.08880336385134768 1102 64-65 0.1452087060368612 1102 66-67 0.2213824453426767 1102 68-69 0.27598613504460445 1102 70-71 0.2801613916737935 1102 72-73 0.16260723155809842 1102 74-75 0.33854522260113384 1102 76-77 0.42819056806154165 1102 78-79 0.35320046602849686 1102 80-81 0.2308225764604579 1102 82-83 0.41827997671123995 1102 84-85 0.2465180455704825 1102 86-87 0.31975339936300884 1102 88-89 0.3333506930704253 1102 90-91 0.4782311276427649 1102 92-93 0.512992718744119 1102 94-95 0.44726614017931254 1102 96-97 0.29147417215981974 1102 98-99 0.28701804290669486 1102 100-101 0.5595173578539985 1103 1 0.028932662325956926 1103 2 0.04346651141194968 1103 3 -0.009989646499178662 1103 4 0.10260373422021019 1103 5 -0.09020721354944783 1103 6 -0.0389566000567072 1103 7 -0.06288670516916994 1103 8 0.03752445531502957 1103 9 0.010686299284841994 1103 10-11 -0.06868602259705625 1103 12-13 -0.01252844452569235 1103 14-15 0.0116823163796127 1103 16-17 -0.04026512318856845 1103 18-19 0.09865405965142315 1103 20-21 0.07747239031390762 1103 22-23 0.12303190950307652 1103 24-25 0.08374340830798843 1103 26-27 0.2352069493643114 1103 28-29 -0.10579404841173101 1103 30-31 -0.055449678306864314 1103 32-33 -0.024258544321050124 1103 34-35 -0.10152740005929672 1103 36-37 0.009552965305928751 1103 38-39 -0.15585112717268856 1103 40-41 -0.13268612377452627 1103 42-43 -0.08995685658528174 1103 44-45 -0.22099210932778846 1103 46-47 -0.2798115209320642 1103 48-49 -0.26360764615236576 1103 50-51 -0.3260342604680275 1103 52-53 -0.32196109741823875 1103 54-55 -0.3041560356844144 1103 56-57 -0.5355673403096617 1103 58-59 -0.34650212568598704 1103 60-61 -0.5638180722848816 1103 62-63 -0.26040202201650686 1103 64-65 -0.42874398738749164 1103 66-67 -0.27018282556912965 1103 68-69 -0.354148021225015 1103 70-71 -0.4447465306818259 1103 72-73 -0.2817054106656869 1103 74-75 -0.29561962948612575 1103 76-77 -0.34601383695257937 1103 78-79 -0.5105209420851224 1103 80-81 -0.4856745251167567 1103 82-83 -0.5789607591252306 1103 84-85 -0.30555364012474584 1103 86-87 -0.2411557784853926 1103 88-89 -0.2198465222858168 1103 90-91 -0.4225757471610656 1103 92-93 -0.3115650388280713 1103 94-95 -0.5168720081650058 1103 96-97 -0.49676808890420787 1103 98-99 -0.444688421900274 1103 100-101 -0.215615180006953 1104 1 0.11550195366392302 1104 2 0.1029849615776115 1104 3 0.0875875981262837 1104 4 0.0026506384248534687 1104 5 0.03855543467609124 1104 6 0.028741801979109027 1104 7 0.0018870586457637728 1104 8 -0.0018251868538143867 1104 9 0.044990007545401056 1104 10-11 0.07183940663141186 1104 12-13 0.04608825855655141 1104 14-15 -0.06060515532661981 1104 16-17 0.02909122855997026 1104 18-19 0.02996021997266496 1104 20-21 -0.043360933198620444 1104 22-23 0.033710629843234585 1104 24-25 0.029077225611004565 1104 26-27 -0.14830524062207218 1104 28-29 -0.09184358302302797 1104 30-31 0.08788018238686135 1104 32-33 0.08941049583979321 1104 34-35 -0.0010363201076657447 1104 36-37 0.03129860309704924 1104 38-39 0.1565086807164846 1104 40-41 -0.05329820400350371 1104 42-43 0.06212531298166368 1104 44-45 0.04120966203267784 1104 46-47 0.2554505022969167 1104 48-49 0.3707104397789607 1104 50-51 0.27344606137180705 1104 52-53 0.3535574611692027 1104 54-55 0.20248165565449483 1104 56-57 0.13871615278866756 1104 58-59 0.3373566604457636 1104 60-61 0.1548776922732955 1104 62-63 0.0029216790630712808 1104 64-65 -0.022984119630628186 1104 66-67 -0.027524472810227962 1104 68-69 -0.09341563996061097 1104 70-71 -0.14806309792258077 1104 72-73 0.021488042858692324 1104 74-75 -0.13152071268255838 1104 76-77 -0.03581676858322069 1104 78-79 -0.040694409075797466 1104 80-81 -0.17884029966073456 1104 82-83 -0.004210620258927378 1104 84-85 0.04081780503543797 1104 86-87 0.22507083717589893 1104 88-89 0.15937141884743866 1104 90-91 0.1467796225061342 1104 92-93 0.23299061650694952 1104 94-95 0.10509974403308675 1104 96-97 0.15767166511976782 1104 98-99 0.19753973994987462 1104 100-101 0.5764163298763201 1105 1 0.06612694796179852 1105 2 0.09498664360061326 1105 3 0.10783822618935801 1105 4 0.10338623319996998 1105 5 -0.05429837280858152 1105 6 0.048479860901338157 1105 7 0.0938115235376813 1105 8 0.03553388773494959 1105 9 0.11702753623227125 1105 10-11 0.12283029069706686 1105 12-13 0.01109815744440823 1105 14-15 0.2237819720551002 1105 16-17 0.19779427934162896 1105 18-19 0.10056413092497252 1105 20-21 0.17948679478475782 1105 22-23 0.22472495275017934 1105 24-25 0.18592379741091491 1105 26-27 0.13449234184307812 1105 28-29 0.07577005098436729 1105 30-31 0.17026882922920805 1105 32-33 0.0870041993674775 1105 34-35 0.09600492014377693 1105 36-37 0.12672189148519664 1105 38-39 0.09018177772060909 1105 40-41 0.2542059575105142 1105 42-43 0.16460990480494075 1105 44-45 0.2399266218507421 1105 46-47 0.1141193071565354 1105 48-49 0.09009874076154745 1105 50-51 0.07317812373273114 1105 52-53 0.025923971328104756 1105 54-55 0.07755495387104361 1105 56-57 0.06795527790446698 1105 58-59 0.14200250253962565 1105 60-61 0.1885384726888404 1105 62-63 0.0818820014010555 1105 64-65 0.0796114044298406 1105 66-67 0.1341888102780544 1105 68-69 0.1264180487705744 1105 70-71 0.28555028228843327 1105 72-73 0.05623181219925755 1105 74-75 0.0907520338171608 1105 76-77 0.09568624504133183 1105 78-79 0.2098561051106742 1105 80-81 0.1465359783408431 1105 82-83 0.08023597509593117 1105 84-85 0.3067549315462941 1105 86-87 0.5622198247689063 1105 88-89 0.5918588886883889 1105 90-91 0.5775952294900151 1105 92-93 0.3234522273176772 1105 94-95 0.3415094045514131 1105 96-97 0.4917995635614112 1105 98-99 0.5403916812847598 1105 100-101 0.5588747292506184 1106 1 -0.1960388328634579 1106 2 -0.0943007612221507 1106 3 -0.13586689005197172 1106 4 -0.008392076533468185 1106 5 -0.15467164338917883 1106 6 -0.22839764954618147 1106 7 -0.1958856674659586 1106 8 -0.11209056960266395 1106 9 -0.29795738417724493 1106 10-11 -0.3914527187293686 1106 12-13 -0.3265893231144119 1106 14-15 -0.428808606920029 1106 16-17 -0.2950635397303287 1106 18-19 -0.28242771392429233 1106 20-21 -0.18303467503577764 1106 22-23 -0.17949124665204153 1106 24-25 -0.24777266328072045 1106 26-27 -0.20127536484197606 1106 28-29 -0.16457224895748368 1106 30-31 -0.22103572440138208 1106 32-33 -0.24767412524268195 1106 34-35 -0.21451022030603895 1106 36-37 -0.19156160481590234 1106 38-39 -0.24470414483926817 1106 40-41 -0.31506886971659753 1106 42-43 -0.6389172316957783 1106 44-45 -0.4998774110944382 1106 46-47 -0.40122535953717886 1106 48-49 -0.25969704236283064 1106 50-51 -0.31001455691805546 1106 52-53 -0.30208011300940996 1106 54-55 -0.3381920606405373 1106 56-57 -0.1597330141121418 1106 58-59 -0.24407761539223571 1106 60-61 -0.14144083513767924 1106 62-63 -0.06983423181191739 1106 64-65 -0.11013515025565113 1106 66-67 -0.15772974640902504 1106 68-69 0.08476456829289702 1106 70-71 0.0841221165887589 1106 72-73 -0.10964909284665225 1106 74-75 -0.23123847212676196 1106 76-77 -0.06961582011872736 1106 78-79 0.00420227300129028 1106 80-81 -0.15955130746811363 1106 82-83 -0.16024494888399587 1106 84-85 -0.18313092339210257 1106 86-87 -0.17798655599413493 1106 88-89 -0.24730274017787224 1106 90-91 -0.4191846355375084 1106 92-93 -0.283822491715064 1106 94-95 -0.6418428627968815 1106 96-97 -0.23204038991501008 1106 98-99 -0.20999646602187738 1106 100-101 0.28058054875535987 1107 1 0.06889196704270972 1107 2 0.08260937824027081 1107 3 0.020069889344242142 1107 4 0.035668488486294336 1107 5 0.04802156141388991 1107 6 0.05145399182605104 1107 7 -0.030807925243493628 1107 8 -0.03881636727371074 1107 9 -0.00906259399051379 1107 10-11 -0.03658968047568578 1107 12-13 -0.03946293059254202 1107 14-15 -0.041100862794401394 1107 16-17 -0.09167191632754879 1107 18-19 0.11183224119633195 1107 20-21 0.10130224776197139 1107 22-23 0.08180649300334863 1107 24-25 -0.026996037731343847 1107 26-27 0.048380515727558304 1107 28-29 0.003718086044436575 1107 30-31 0.011719798106902601 1107 32-33 -0.10779986714646839 1107 34-35 -0.022444220190962483 1107 36-37 -0.24015082580032754 1107 38-39 -0.16853225773699876 1107 40-41 -0.07735315883888205 1107 42-43 -0.0908715202426933 1107 44-45 0.10042466158889596 1107 46-47 0.1917528336919645 1107 48-49 0.15820668386416514 1107 50-51 0.01842962491863176 1107 52-53 0.07077738392560207 1107 54-55 0.030587787017466894 1107 56-57 0.15515728813758045 1107 58-59 0.12634692397458736 1107 60-61 0.29946799423662895 1107 62-63 0.1812620817948627 1107 64-65 0.2609581536892236 1107 66-67 0.19682944877197173 1107 68-69 0.10916761513297502 1107 70-71 0.08074377110612829 1107 72-73 -0.0936996551665743 1107 74-75 -0.045441863307566166 1107 76-77 -0.003452386625614423 1107 78-79 -0.13606899490730484 1107 80-81 -0.043577084337091065 1107 82-83 0.08216605585622005 1107 84-85 0.07667305024680005 1107 86-87 -0.05028881456835066 1107 88-89 0.10702984516595748 1107 90-91 0.21698530413647532 1107 92-93 0.20545558776656492 1107 94-95 0.03010155582938623 1107 96-97 0.15287345851310263 1107 98-99 0.383000094996806 1107 100-101 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72-73 -0.7169863561101764 2316 74-75 -0.7139605161682603 2316 76-77 -0.6022897852271605 2316 78-79 -0.6148916332179155 2316 80-81 -0.5434051475115922 2316 82-83 -0.5825828392640275 2316 84-85 -0.943957940766218 2316 86-87 -0.8530398608981855 2316 88-89 -0.6574378389935198 2316 90-91 -0.6079604443685049 2316 92-93 -0.6518234234310363 2316 94-95 -0.7579660284810643 2316 96-97 -0.7022651962554569 2316 98-99 -0.47961267071270797 2316 100-101 -1.1344956309702603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 11.0 5 27.0 6 55.0 7 119.0 8 296.0 9 553.0 10 1044.0 11 1580.0 12 2407.0 13 3465.0 14 4522.0 15 6221.0 16 8354.0 17 11390.0 18 10292.0 19 6558.0 20 5751.0 21 5945.0 22 6329.0 23 6842.0 24 7894.0 25 9890.0 26 12101.0 27 15213.0 28 19446.0 29 24506.0 30 31024.0 31 39373.0 32 50500.0 33 67645.0 34 92713.0 35 132796.0 36 205159.0 37 318056.0 38 263582.0 39 23417.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.433829040274546 19.844797228726858 15.240998203405997 22.4803755275926 2 15.794257402606155 20.600136335974277 39.68165334965733 23.92395291176224 3 19.581200545057175 24.450803285539283 29.891554839389485 26.076441330014056 4 13.202870366852725 17.117977926761345 37.22688900947112 32.45226269691481 5 13.710915824655128 37.46130474867264 34.27836492758486 14.549414499087373 6 31.94806330519403 36.64527486895045 17.24621402454191 14.160447801313614 7 28.718864755717043 32.5126892091725 20.628665104513495 18.13978093059696 8 26.544800560253407 35.44928868031508 19.44163895624848 18.564271803183036 9 26.015298014526735 16.910463391783857 20.199084928997056 36.87515366469235 10-11 24.936258272805272 27.05792415218306 27.214832379148767 20.7909851958629 12-13 25.834863463852304 24.01698597202146 28.126242129669286 22.02190843445695 14-15 22.471960449665 25.66524715733759 26.308470535444844 25.55432185755256 16-17 22.074923140773702 29.021033157166993 26.929114611818793 21.974929090240508 18-19 21.913964975560592 27.403781710478874 29.298722728325128 21.38353058563541 20-21 22.511563828101984 26.430649645935045 29.716762371844542 21.34102415411843 22-23 22.179675709075035 26.404037920555496 29.548421965995363 21.867864404374103 24-25 22.142915767226334 26.59273067038067 29.675742725957395 21.588610836435603 26-27 22.08140884737284 26.838447833602558 29.4598584514965 21.620284867528106 28-29 21.802123662138012 26.9182383625451 29.68385203896941 21.595785936347482 30-31 21.997042469874554 26.61662424386219 29.698017967389756 21.6883153188735 32-33 21.916509627025775 26.78923046155677 29.520680848379634 21.773579063037825 34-35 22.26785073002825 26.703572475616145 29.387570488637593 21.64100630571801 36-37 22.194199198183906 26.622967952444405 29.4537672668938 21.729065582477887 38-39 22.00897724364787 26.842453101356696 29.47068382311303 21.677885831882403 40-41 22.192048788495022 26.75123989038645 29.409755548594603 21.646955772523928 42-43 22.0058391177878 26.790904740653914 29.24387188351874 21.95938425803954 44-45 22.200901308380935 26.83302713888708 29.033577213685497 21.932494339046492 46-47 22.242941817798652 27.098602735464482 28.66632307898527 21.992132367751598 48-49 22.273226754250462 27.17684180797845 28.518876654650654 22.031054783120428 50-51 22.34849109336147 27.164226071136987 28.457912539970742 22.029370295530804 52-53 22.49393046865312 27.399050809163327 28.113416907811217 21.993601814372337 54-55 22.28186423316749 27.239347049602063 28.30874578788502 22.170042929345424 56-57 22.326771955503723 27.476429717801736 28.216708253200707 21.980090073493834 58-59 22.431640267998393 27.259094978578336 28.305090091413916 22.00417466200936 60-61 22.36225371537034 27.30307085671605 28.63123556089494 21.703439867018666 62-63 22.3356961557126 27.342996796606368 28.427305042065598 21.894002005615437 64-65 22.1038819912507 27.323678949567874 28.367201091261236 22.205237967920187 66-67 22.163627540440245 27.510979633469834 28.193304627753328 22.132088198336586 68-69 22.092843221514418 27.315794114041957 28.35017701455756 22.241185649886063 70-71 22.14398713194842 27.337119010123413 28.33297373704647 22.185920120881697 72-73 22.223604060549846 27.226930029919387 28.306006385287745 22.24345952424302 74-75 22.13470453012473 27.603053008315637 27.936689071546283 22.325553390013354 76-77 22.220398356226834 27.394355748009257 28.08954736026459 22.295698535499323 78-79 22.14542073840768 27.43388744612328 28.08736111041422 22.333330705054824 80-81 22.10940137611884 27.59240848035565 28.034389351744665 22.263800791780845 82-83 22.14091664994122 27.550355535166442 28.08107778765375 22.22765002723858 84-85 22.18155113697048 27.40814598868728 27.918229016957607 22.49207385738463 86-87 22.171870777580978 27.295759463773837 28.01704271358765 22.515327045057536 88-89 22.08155357064777 27.275258891406462 28.069441029673502 22.57374650827227 90-91 22.1215027771326 27.344755850330188 28.0547024869459 22.47903888559131 92-93 22.12849534431964 27.19755997906915 28.093106439103416 22.580838237507795 94-95 22.005464907822688 27.035703252064575 28.179040243483723 22.779791596629018 96-97 21.940307494249446 27.14870728121553 28.191154218064447 22.719831006470585 98-99 21.889194964306732 27.006711438260574 28.064212758972417 23.039880838460274 100-101 21.65683998404217 27.226093991332164 28.157361696118038 22.959704328507634 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 167.0 1 132.5 2 134.5 3 195.5 4 173.0 5 159.0 6 314.0 7 690.5 8 1100.0 9 1379.0 10 1395.0 11 1287.0 12 1360.5 13 1489.5 14 1645.0 15 1773.0 16 1864.0 17 1969.5 18 2101.0 19 2253.0 20 2430.0 21 2739.0 22 3182.5 23 3755.5 24 4565.5 25 5629.0 26 7136.0 27 9220.0 28 11282.0 29 13883.5 30 16880.0 31 19075.5 32 21691.5 33 24537.5 34 27736.0 35 30955.0 36 34438.5 37 37821.0 38 40172.5 39 42130.5 40 43641.5 41 45926.5 42 48571.0 43 50992.5 44 52987.0 45 54455.5 46 54754.0 47 55016.0 48 57582.0 49 57388.5 50 54375.5 51 51725.0 52 48855.0 53 46340.5 54 43862.5 55 40408.5 56 35993.0 57 31749.0 58 27940.0 59 24625.0 60 21050.5 61 16712.0 62 12719.0 63 9719.5 64 7257.0 65 5468.5 66 4033.5 67 3108.5 68 2178.0 69 1334.5 70 979.5 71 728.5 72 529.0 73 398.0 74 308.5 75 252.0 76 175.5 77 80.5 78 44.5 79 24.5 80 11.5 81 8.5 82 5.0 83 1.5 84 1.5 85 1.0 86 0.5 87 0.5 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05576729126510752 2 0.0 3 0.0 4 0.0 5 0.014981187499238395 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035481759866617256 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.092827451843367E-4 24-25 1.7920080740715786E-4 26-27 9.676843599986524E-4 28-29 0.0028672129185145258 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012938298294796795 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.168032296286315E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1504096888858942E-4 84-85 0.0011468851674058104 86-87 0.0 88-89 0.0 90-91 0.008529958432580713 92-93 9.318441985172209E-4 94-95 0.0 96-97 0.0 98-99 1.433606459257263E-4 100-101 0.011217970543688081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1395083.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.1543060445943 #Duplication Level Percentage of deduplicated Percentage of total 1 82.85115761069335 53.98109679116033 2 10.461659988910187 13.632443933038827 3 2.8593262513889455 5.588922529930137 4 1.2362151833105537 3.2217896956156027 5 0.6384453520610541 2.079873193046734 6 0.39382883620741316 1.539578671406652 7 0.26475321650259365 1.2074868466010504 8 0.21361066960124117 1.1134123953271986 9 0.14861444871667165 0.871458414690122 >10 0.8453056457076918 10.055801732909252 >50 0.06119394262606508 2.6862130029271163 >100 0.02511771659753712 2.8511905114653886 >500 2.203250504519247E-4 0.09438495191431262 >1k 4.406501009038494E-4 0.6964389265586125 >5k 1.1016252522596235E-4 0.37990840340871235 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5293 0.3794039494424346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4462 0.31983760106029535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2991 0.21439584598192366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.168032296286314E-5 0.0 10-11 0.0 0.0 0.0 7.168032296286314E-5 0.0 12-13 0.0 0.0 0.0 7.168032296286314E-5 0.0 14-15 0.0 0.0 0.0 7.168032296286314E-5 0.0 16-17 0.0 0.0 0.0 7.168032296286314E-5 0.0 18-19 0.0 0.0 0.0 7.168032296286314E-5 0.0 20-21 0.0 0.0 0.0 7.168032296286314E-5 0.0 22-23 0.0 0.0 0.0 7.168032296286314E-5 0.0 24-25 0.0 0.0 0.0 2.50881130370021E-4 0.0 26-27 0.0 0.0 0.0 7.168032296286314E-4 0.0 28-29 0.0 0.0 0.0 0.0011468851674058104 0.0 30-31 0.0 0.0 0.0 0.0020428892044415995 0.0 32-33 0.0 0.0 0.0 0.0036198563096245884 0.0 34-35 0.0 0.0 0.0 0.005591065191103325 0.0 36-37 0.0 0.0 0.0 0.0077773150414706515 0.0 38-39 0.0 0.0 0.0 0.009856044407393683 0.0 40-41 0.0 0.0 0.0 0.012902458133315364 0.0 42-43 0.0 0.0 0.0 0.01756167912590147 0.0 44-45 0.0 0.0 0.0 0.02641419901181507 0.0 46-47 0.0 0.0 0.0 0.04365331668438365 0.0 48-49 0.0 0.0 0.0 0.07899171590507517 0.0 50-51 0.0 0.0 0.0 0.1070545623450361 0.0 52-53 0.0 0.0 0.0 0.13275195812722254 0.0 54-55 0.0 0.0 0.0 0.16271433312569933 0.0 56-57 0.0 0.0 0.0 0.18636883970344417 0.0 58-59 0.0 0.0 0.0 0.22052451359524844 0.0 60-61 0.0 0.0 0.0 0.27998334149294346 0.0 62-63 0.0 0.0 0.0 0.3309122109580577 0.0 64-65 0.0 0.0 0.0 0.38904495288093965 0.0 66-67 0.0 0.0 0.0 0.48326873741562326 0.0 68-69 0.0 0.0 0.0 0.5876352876495521 0.0 70-71 0.0 0.0 0.0 0.691105833846445 0.0 72-73 0.0 0.0 0.0 0.799486482166294 0.0 74-75 0.0 0.0 0.0 0.9022043849720769 0.0 76-77 0.0 0.0 0.0 0.9971808128978705 0.0 78-79 0.0 0.0 0.0 1.108249473328827 0.0 80-81 0.0 0.0 0.0 1.2883462847730205 0.0 82-83 0.0 0.0 0.0 1.4765071325505366 0.0 84-85 0.0 0.0 0.0 1.6748107460272972 0.0 86-87 0.0 0.0 0.0 1.9624638820772673 0.0 88-89 0.0 0.0 0.0 2.335165721322674 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4080 0.0 36.463146 1 GTATCAA 6405 0.0 33.3194 1 GCGTTCG 60 5.3871718E-6 27.707088 34-35 ATCAACG 7655 0.0 27.61638 3 TCAACGC 7705 0.0 27.43717 4 CAACGCA 7805 0.0 27.146502 5 TATCAAC 7860 0.0 26.835663 2 AACGCAG 7995 0.0 26.437212 6 GTACATG 5005 0.0 25.640688 1 TACATGG 5130 0.0 24.540335 2 ACATGGG 4935 0.0 23.969784 3 TACCTGG 1080 0.0 23.753191 2 CGTTCGG 70 1.781282E-5 23.748934 34-35 GTACCTG 1105 0.0 23.657583 1 ACGCAGA 9030 0.0 23.197464 7 CGCAGAG 9190 0.0 22.741905 8 GAGTACT 5185 0.0 22.490154 12-13 GTACTTT 5455 0.0 21.24561 14-15 GCAGAGT 9725 0.0 20.758167 9 CATGGGG 2660 0.0 20.359877 4 >>END_MODULE