FastQCFastQC Report
Thu 26 May 2016
SRR937252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502222
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45770.3046819977340233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.23431956128987594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23030.1533062356961887No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21090.14039203260237168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.035.5469361
GTATCAA59300.032.5381
ATCAACG70950.027.0549643
TCAACGC71000.027.035914
TATCAAC72900.026.3964482
CAACGCA72850.026.2850025
AACGCAG74850.025.8339946
GTACATG59350.025.7842581
TACATGG60000.024.5486972
ACATGGG59550.023.8565373
ACGCAGA85600.022.2551567
CGCAGAG86250.022.0844848
GAGTACT42950.021.62076812-13
CATGGGG31350.021.2181954
TAGACCG1150.003716906320.658785
GCAGAGT91650.020.4211529
GTACTTT46000.019.98069814-15
AGTACTT46950.019.47523312-13
GTACCTG13200.019.4419271
TACCTGG12550.019.3083172
Produced by FastQC (version 0.11.5)