FastQCFastQC Report
Thu 26 May 2016
SRR937252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502222
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.37038467017524707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.31080625899500874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30980.20622784115796466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.037.924491
GTATCAA63650.030.7586731
ACCGTAT1301.0015681E-525.5795758
ATCAACG76900.025.2008173
TCAACGC77650.024.8962384
TATCAAC78750.024.7897472
CAACGCA78150.024.7369565
GTACATG54650.024.5204091
AACGCAG81200.023.8077966
TACATGG56400.023.4124322
ACATGGG56350.022.7588713
CATGGGG30750.021.4709134
ACGCAGA91300.021.1248637
TACCTGG13150.020.9499552
CGCAGAG92050.020.797928
GAGTACT50900.020.67257712-13
GTACCTG13550.019.6389161
GTACTTT53550.019.51389714-15
AGTACTT53550.019.25036212-13
GCAGAGT97350.019.1776459
Produced by FastQC (version 0.11.5)