##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937252_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1502222 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20225572518576 33.0 31.0 34.0 31.0 34.0 2 32.41320723568155 34.0 31.0 34.0 31.0 34.0 3 32.47385739258245 34.0 31.0 34.0 31.0 34.0 4 36.112077975159465 37.0 35.0 37.0 35.0 37.0 5 36.03352034519532 37.0 35.0 37.0 35.0 37.0 6 36.07236746632655 37.0 35.0 37.0 35.0 37.0 7 36.01722381911595 37.0 35.0 37.0 35.0 37.0 8 35.994909540667095 37.0 35.0 37.0 35.0 37.0 9 37.70626844767285 39.0 37.0 39.0 35.0 39.0 10-11 37.625417215298405 39.0 37.0 39.0 35.0 39.0 12-13 37.52582574346535 39.0 37.0 39.0 35.0 39.0 14-15 38.74840469650957 40.0 38.0 41.0 35.0 41.0 16-17 38.71424097104156 40.0 38.0 41.0 35.0 41.0 18-19 38.685661639890775 40.0 38.0 41.0 34.5 41.0 20-21 38.585075308443095 40.0 38.0 41.0 34.0 41.0 22-23 38.43638556751266 40.0 38.0 41.0 34.0 41.0 24-25 38.382623207488635 40.0 38.0 41.0 34.0 41.0 26-27 38.350604970503696 40.0 38.0 41.0 34.0 41.0 28-29 38.23585928045256 40.0 38.0 41.0 34.0 41.0 30-31 38.329241949592 40.0 38.0 41.0 34.0 41.0 32-33 38.29131746173336 40.0 38.0 41.0 34.0 41.0 34-35 38.17706104690252 40.0 38.0 41.0 34.0 41.0 36-37 38.026327999456804 40.0 37.5 41.0 33.0 41.0 38-39 37.920101023683586 40.0 37.0 41.0 33.0 41.0 40-41 37.75444574769907 40.0 37.0 41.0 33.0 41.0 42-43 37.56435866336666 40.0 37.0 41.0 33.0 41.0 44-45 37.32095256227109 40.0 37.0 41.0 32.0 41.0 46-47 37.019549374193694 40.0 36.5 41.0 31.5 41.0 48-49 36.73650232788496 39.5 36.0 41.0 31.0 41.0 50-51 36.51382385559525 39.0 36.0 41.0 30.5 41.0 52-53 36.28163979757986 39.0 35.5 40.5 30.0 41.0 54-55 36.052662322879044 39.0 35.0 40.0 29.5 41.0 56-57 35.7913943478394 38.5 35.0 40.0 29.0 41.0 58-59 35.52479094301641 38.0 35.0 40.0 28.0 41.0 60-61 35.20178076209774 38.0 34.0 40.0 28.0 41.0 62-63 34.83846561959551 37.5 34.0 40.0 27.5 41.0 64-65 34.441462380393844 37.0 34.0 40.0 26.5 41.0 66-67 34.02443447107019 36.5 33.0 39.0 26.0 40.5 68-69 33.59500127144989 36.0 33.0 39.0 26.0 40.0 70-71 33.18162761562539 35.5 32.5 38.5 25.0 40.0 72-73 32.706751066087435 35.0 32.0 38.0 24.5 40.0 74-75 32.22990044081368 35.0 31.5 37.0 23.0 39.0 76-77 30.965022147192627 34.0 30.0 36.0 21.5 38.5 78-79 31.573339027121158 34.0 31.0 36.0 23.0 38.5 80-81 31.37866274092644 34.0 31.0 36.0 22.0 37.5 82-83 31.025605403196067 34.0 31.0 35.0 20.5 37.0 84-85 30.656687227320596 34.0 31.0 35.0 19.5 36.5 86-87 30.236074960957836 34.0 30.0 35.0 18.0 36.0 88-89 29.77429634235153 34.0 30.0 35.0 14.0 36.0 90-91 29.253570044906812 34.0 29.0 35.0 7.0 35.0 92-93 28.876521912207384 34.0 29.0 35.0 2.0 35.0 94-95 28.416855830895834 33.5 29.0 35.0 2.0 35.0 96-97 27.91692373031416 33.0 28.5 35.0 2.0 35.0 98-99 27.131727201438935 33.0 26.5 35.0 2.0 35.0 100-101 24.56131151054904 30.5 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10542785170977709 1101 2 0.08588438888151018 1101 3 0.055121486332467384 1101 4 0.01846855315168483 1101 5 -0.012291420966178634 1101 6 0.0015895895395985349 1101 7 -0.058752536900129826 1101 8 -0.11184572329187148 1101 9 0.06051795223565648 1101 10-11 0.09266516360259658 1101 12-13 0.002910932385056242 1101 14-15 -0.22424129931098946 1101 16-17 -0.05970743521361044 1101 18-19 0.024189829832721443 1101 20-21 0.08431817344383319 1101 22-23 -0.012285718823711989 1101 24-25 -0.10657272611987167 1101 26-27 0.026293910058676317 1101 28-29 -0.09098457258141934 1101 30-31 -0.029701772571861795 1101 32-33 0.009982185854461534 1101 34-35 0.0934300241200603 1101 36-37 0.11821553854758093 1101 38-39 0.01111217436385914 1101 40-41 0.10814489881365574 1101 42-43 0.050453343934862005 1101 44-45 0.03211457474276358 1101 46-47 -0.18655368777935877 1101 48-49 -0.16018665345502114 1101 50-51 0.001213930058099777 1101 52-53 -0.17519964448860037 1101 54-55 -0.34107666924445823 1101 56-57 -0.2679849188457055 1101 58-59 -0.18717234486130252 1101 60-61 -0.15180548010312833 1101 62-63 -0.21775687490934814 1101 64-65 -0.23182324702806056 1101 66-67 -0.2119243736496088 1101 68-69 -0.1994471862722449 1101 70-71 -0.07476609359733999 1101 72-73 -0.15616569734308428 1101 74-75 -0.23972360778300583 1101 76-77 -0.2595171590238152 1101 78-79 -0.3641091176684981 1101 80-81 -0.20954231855862204 1101 82-83 -0.17093753664268263 1101 84-85 -0.28943869866548866 1101 86-87 -0.1868703213174605 1101 88-89 -0.012366910575007495 1101 90-91 -0.1282618084089755 1101 92-93 -0.20338501569473166 1101 94-95 -0.13146365976418295 1101 96-97 -0.34638920483302016 1101 98-99 -0.15790365434149933 1101 100-101 0.6411048731790281 1102 1 -0.07240466360708808 1102 2 0.004802504975870647 1102 3 -0.08090878789346334 1102 4 -0.025746227202510852 1102 5 -0.02735008356256685 1102 6 -0.05833087854252739 1102 7 -0.11876783091383203 1102 8 -0.09306181064445695 1102 9 -0.13629955240281788 1102 10-11 -0.13916672106469719 1102 12-13 -0.09277433539728719 1102 14-15 -0.11234270136380076 1102 16-17 -0.1619990999761427 1102 18-19 -0.13927712809046255 1102 20-21 -0.08154418652606665 1102 22-23 -0.09353554745154469 1102 24-25 -0.16387462751308846 1102 26-27 -0.1734239732741898 1102 28-29 -0.3078560480272188 1102 30-31 -0.19384012855678634 1102 32-33 -0.09168016223363651 1102 34-35 -0.17361331856638884 1102 36-37 -0.14675077349294696 1102 38-39 -0.17730561878917683 1102 40-41 -0.13285558712163947 1102 42-43 -0.2814880665875421 1102 44-45 -0.30006866094651485 1102 46-47 -0.14131238815378566 1102 48-49 0.012996344598185772 1102 50-51 -8.168301031261649E-4 1102 52-53 -0.06207821093378385 1102 54-55 0.02368403428017274 1102 56-57 -0.0705497127573409 1102 58-59 0.035227787057181104 1102 60-61 -0.011508038956137057 1102 62-63 -0.036134178532087446 1102 64-65 -0.13790139882876673 1102 66-67 0.008191367302700314 1102 68-69 0.012115750726771068 1102 70-71 0.02203233160670237 1102 72-73 -0.029719506679739993 1102 74-75 0.0655886627687039 1102 76-77 0.05525923457463122 1102 78-79 -0.0415147258797397 1102 80-81 -0.014923518803570346 1102 82-83 -0.038673260960468525 1102 84-85 -0.07715390402265498 1102 86-87 -0.15837798508070122 1102 88-89 0.05776276864676433 1102 90-91 0.05551046616427158 1102 92-93 0.09222073540280817 1102 94-95 0.24429399562086473 1102 96-97 0.16216931152578695 1102 98-99 0.34430780824758145 1102 100-101 1.0632188897465262 1103 1 0.008918355129033273 1103 2 -0.010751464897332141 1103 3 -0.09644701753740748 1103 4 -0.00315375148181829 1103 5 -0.06346304144395987 1103 6 -0.05344353735956986 1103 7 -0.08382302290340249 1103 8 -0.09239584904012332 1103 9 -0.025882968497278114 1103 10-11 -0.01859843882589729 1103 12-13 -0.08967425976923238 1103 14-15 -0.16995756193909983 1103 16-17 -0.23088490161477893 1103 18-19 -0.11819646391275285 1103 20-21 -0.17640848701153544 1103 22-23 -0.11639110986117629 1103 24-25 -0.11964308424357029 1103 26-27 -0.16588777636842877 1103 28-29 -0.2619253095331402 1103 30-31 -0.317253457233015 1103 32-33 -0.25613482700048706 1103 34-35 -0.14914344573994498 1103 36-37 -0.02153818670620211 1103 38-39 -0.08076891894560134 1103 40-41 -0.16629570416898787 1103 42-43 -0.18008688637016235 1103 44-45 -0.13453991291685696 1103 46-47 -0.090755521328866 1103 48-49 -0.13985608475629618 1103 50-51 -0.04642920372220516 1103 52-53 -0.05855558510376113 1103 54-55 -0.229742276337511 1103 56-57 -0.2799995092961396 1103 58-59 -0.31458569122617774 1103 60-61 -0.29255826452861555 1103 62-63 -0.36314643567346394 1103 64-65 -0.39851274933293723 1103 66-67 -0.17972916852468046 1103 68-69 -0.12964206560874203 1103 70-71 -0.1522234366251709 1103 72-73 -0.18100896874616268 1103 74-75 -0.021524136960863416 1103 76-77 0.10755202382570772 1103 78-79 0.09583488934660878 1103 80-81 -0.036271204727530204 1103 82-83 0.13144132790849028 1103 84-85 0.11218156598433637 1103 86-87 0.14815361043138253 1103 88-89 0.2423593053466071 1103 90-91 0.05865626341062935 1103 92-93 0.15585192384258661 1103 94-95 0.45224303625388984 1103 96-97 0.4836934754010649 1103 98-99 0.581445042809225 1103 100-101 1.338512876004554 1104 1 0.006178286140496425 1104 2 0.10217554077753022 1104 3 0.005102442794182593 1104 4 -0.004377741689893355 1104 5 0.054387743022488166 1104 6 0.03823054742957055 1104 7 0.031287692574508696 1104 8 0.08124385275135637 1104 9 0.046360271738620895 1104 10-11 0.038664830340671585 1104 12-13 0.0783114417997055 1104 14-15 0.0778100468291214 1104 16-17 -0.07217648946389232 1104 18-19 0.03679129618517152 1104 20-21 0.12370556662148857 1104 22-23 0.10682714984728392 1104 24-25 0.23636420406175773 1104 26-27 0.03964521573127655 1104 28-29 0.13980079483984298 1104 30-31 0.12276406808133089 1104 32-33 0.17055789582136782 1104 34-35 0.23719237266442406 1104 36-37 0.10611842840452113 1104 38-39 0.10941551412438599 1104 40-41 0.12910320536701647 1104 42-43 0.019882513874634355 1104 44-45 0.20231471042797722 1104 46-47 0.1091582429973812 1104 48-49 0.046086109524331675 1104 50-51 0.09057424570475092 1104 52-53 0.10507660583870404 1104 54-55 0.1906427242188471 1104 56-57 0.3517602548075658 1104 58-59 0.20076982229335272 1104 60-61 0.13998318423070089 1104 62-63 0.23588549934378733 1104 64-65 0.29737436538832185 1104 66-67 0.34376309808263983 1104 68-69 0.42918596376702567 1104 70-71 0.5751883804439331 1104 72-73 0.5177851227192889 1104 74-75 0.5025801247504944 1104 76-77 0.4927055789609014 1104 78-79 0.31179961617424823 1104 80-81 0.25247921254185357 1104 82-83 0.1983017930247719 1104 84-85 0.29790121659076974 1104 86-87 0.4086270720982377 1104 88-89 0.43162574576053814 1104 90-91 0.34287235331827404 1104 92-93 0.48065441633173833 1104 94-95 0.7369737584081157 1104 96-97 0.7659066722409413 1104 98-99 0.9524462111635152 1104 100-101 1.5972407607269332 1105 1 -0.014916373112058068 1105 2 -0.01437956748237923 1105 3 0.007884843459876834 1105 4 -0.01034595246360226 1105 5 -0.01065886693258733 1105 6 -0.05537660767644326 1105 7 -0.11178350427243089 1105 8 -0.018394062676037493 1105 9 -0.05696455298208747 1105 10-11 -0.04569342535625509 1105 12-13 0.010645833087188805 1105 14-15 0.0029069634878240436 1105 16-17 -0.004007673154340807 1105 18-19 -0.09652709085175104 1105 20-21 -0.026356420683065096 1105 22-23 -0.017667885545911588 1105 24-25 -0.11426853029523443 1105 26-27 0.10811307510302726 1105 28-29 -0.0960422746702676 1105 30-31 -0.03658933826864086 1105 32-33 0.036048607564943325 1105 34-35 0.0798416060459175 1105 36-37 0.09926468470866467 1105 38-39 0.15179353547838303 1105 40-41 0.12846084259650326 1105 42-43 0.08489117241875732 1105 44-45 0.16656131869632418 1105 46-47 0.22653984865129928 1105 48-49 0.3476026900740976 1105 50-51 0.32461638020455297 1105 52-53 0.35957982186938153 1105 54-55 0.6001806165942654 1105 56-57 0.562454540730684 1105 58-59 0.47231919567338565 1105 60-61 0.46100151897901753 1105 62-63 0.44017372765947727 1105 64-65 0.561342241627365 1105 66-67 0.5018525116068417 1105 68-69 0.4826640700197302 1105 70-71 0.4728896494266692 1105 72-73 0.48774130992376286 1105 74-75 0.4991258435502175 1105 76-77 0.6294677051395041 1105 78-79 0.5466872237735281 1105 80-81 0.4017238389031874 1105 82-83 0.3642288757138701 1105 84-85 0.3445951567343677 1105 86-87 0.34380254817760303 1105 88-89 0.5069526198574366 1105 90-91 0.7700674910397254 1105 92-93 0.7224569115667272 1105 94-95 0.8229793864617996 1105 96-97 0.9448152458863568 1105 98-99 0.8844221724586951 1105 100-101 1.4899496621600896 1106 1 -0.15982281801850462 1106 2 -0.010999652450294661 1106 3 0.060085512356195636 1106 4 -0.03887166881540338 1106 5 -0.09007710791604495 1106 6 -0.11056923173862998 1106 7 -0.01710538802910122 1106 8 -0.06580362608559653 1106 9 -0.12298606613403251 1106 10-11 -0.15797815824968353 1106 12-13 -0.1694046053110796 1106 14-15 -0.07071718909285352 1106 16-17 -0.10324513134832358 1106 18-19 -0.14116416940187548 1106 20-21 -0.07781117978999674 1106 22-23 -0.1090629863323258 1106 24-25 -0.19660984820176708 1106 26-27 -0.06604746001403328 1106 28-29 -0.13118319485467111 1106 30-31 -0.12598841627642088 1106 32-33 -0.21767931433689114 1106 34-35 -0.15937958169701005 1106 36-37 -0.07246088701690212 1106 38-39 -0.2551073051615944 1106 40-41 -0.1093914578644899 1106 42-43 -0.2584211298500563 1106 44-45 -0.011940534327273156 1106 46-47 -0.11846891931399028 1106 48-49 -0.25648860523458694 1106 50-51 -0.15051224675016783 1106 52-53 -0.0867229893029986 1106 54-55 -0.18844597655406403 1106 56-57 -0.206594180231086 1106 58-59 -0.15045726640742174 1106 60-61 -0.12849970130481125 1106 62-63 0.14060688322306447 1106 64-65 -0.009155814957651387 1106 66-67 -0.03150263618309168 1106 68-69 -0.004771526111518654 1106 70-71 -0.07312372849975901 1106 72-73 -0.012392830906037489 1106 74-75 -0.06903378765289148 1106 76-77 0.06444610159616104 1106 78-79 -0.01255113833439836 1106 80-81 -0.11365770317400603 1106 82-83 -0.25789658754202804 1106 84-85 -0.14268822028813943 1106 86-87 -0.014981687563153656 1106 88-89 0.0029652746527020213 1106 90-91 0.05358932056155652 1106 92-93 0.21204418445834605 1106 94-95 0.31548792844860074 1106 96-97 0.4655761606472737 1106 98-99 0.7000875435153695 1106 100-101 1.11359243536808 1107 1 -0.14268785717096932 1107 2 -0.0936178007690387 1107 3 -0.06558560694366378 1107 4 -0.09646724689002184 1107 5 -0.042510088109501964 1107 6 -0.13768484666262992 1107 7 -0.15847249995908896 1107 8 -0.03242545934216423 1107 9 -0.14434396571947872 1107 10-11 -0.02011450094889966 1107 12-13 -0.11317890286980514 1107 14-15 -0.10143206702928609 1107 16-17 -0.023794187083005625 1107 18-19 -0.03598683176944917 1107 20-21 -0.04130818577802131 1107 22-23 0.03203236143368571 1107 24-25 0.024239529964972917 1107 26-27 -0.019757271160962375 1107 28-29 -0.16271797145531508 1107 30-31 -0.06798565257305 1107 32-33 -0.03814730143960787 1107 34-35 -0.0377453117753106 1107 36-37 0.0766639402516418 1107 38-39 0.09929066812261311 1107 40-41 -0.032689477210411155 1107 42-43 0.036483522383932154 1107 44-45 0.10474394287218303 1107 46-47 0.09405710555933666 1107 48-49 0.0206896536322958 1107 50-51 -0.005686820679237314 1107 52-53 -0.1108980238492876 1107 54-55 0.006529610732897595 1107 56-57 -0.010020251166132255 1107 58-59 -0.08933704531114728 1107 60-61 -0.0020676423458070303 1107 62-63 0.013562203352030622 1107 64-65 0.1264452905236979 1107 66-67 0.15352085529548276 1107 68-69 0.27176002616853623 1107 70-71 0.33690943594059064 1107 72-73 0.5079102552293833 1107 74-75 0.43435174896617923 1107 76-77 0.47323375705447646 1107 78-79 0.23925355996131614 1107 80-81 0.36643073533359427 1107 82-83 0.2869481844524415 1107 84-85 0.43742324908081187 1107 86-87 0.40826105771551724 1107 88-89 0.5789228978678089 1107 90-91 0.6199946637213927 1107 92-93 0.6098940232423189 1107 94-95 0.6973976474720907 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0.08217982804862345 2316 74-75 -0.13347691861632427 2316 76-77 -0.1723077614452997 2316 78-79 -0.37788559750859463 2316 80-81 -0.31032442613271627 2316 82-83 -0.17955799090266567 2316 84-85 -0.16402774574622114 2316 86-87 -0.5030944968175675 2316 88-89 -0.4788947270360602 2316 90-91 -0.24937209124288628 2316 92-93 -0.22311109714259914 2316 94-95 -0.3720230062807772 2316 96-97 0.043081184712118414 2316 98-99 -0.10539383789136636 2316 100-101 -0.4126644252246727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 6.0 5 37.0 6 51.0 7 117.0 8 320.0 9 628.0 10 998.0 11 1721.0 12 2587.0 13 3626.0 14 4795.0 15 6481.0 16 8594.0 17 11897.0 18 11068.0 19 7231.0 20 6524.0 21 6568.0 22 7146.0 23 7948.0 24 9286.0 25 11248.0 26 13572.0 27 17148.0 28 21502.0 29 27264.0 30 34359.0 31 43613.0 32 55868.0 33 73503.0 34 101517.0 35 145043.0 36 222943.0 37 342073.0 38 272037.0 39 22898.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.577242601823805 19.701934587352206 15.089261744519359 22.63156106630463 2 15.849588143430198 20.632769324374163 39.58702508683803 23.930617445357612 3 19.558959993928994 24.387141181529763 29.813636067105925 26.240262757435318 4 13.135608452013084 17.10259868381637 37.086396018697634 32.6753968454729 5 13.767497035980302 37.380199349879476 34.28663485074721 14.565668763393008 6 32.02962012272487 36.65596696094186 17.17322739248926 14.141185523844014 7 28.785692128060962 32.460515156880945 20.564670201874293 18.189122513183804 8 26.531897415961158 35.43424340743246 19.37663008530031 18.65722909130608 9 26.01732633392401 16.932783569938398 20.213190859939477 36.836699236198115 10-11 25.026527406105632 26.993416410379307 27.164929238061003 20.81512694545406 12-13 25.764429647707626 23.996976299701824 28.131031007241035 22.10756304534951 14-15 22.522137207416744 25.60290689392114 26.197892189037308 25.677063709624807 16-17 22.11730356764846 28.98070325158332 26.85385382453459 22.04813935623363 18-19 21.997081656373027 27.393787336359072 29.175647807048495 21.43348320021941 20-21 22.58793973194375 26.431379649612374 29.51278173266002 21.46789888578386 22-23 22.253938446043346 26.384285025301047 29.40390178340519 21.957874745250415 24-25 22.223997379867317 26.628196781550432 29.449432637427158 21.698373201155093 26-27 22.132554750745065 26.84379154276646 29.322894095859652 21.700759610628825 28-29 21.79431405788327 26.91704081771907 29.54435841013568 21.74428671426199 30-31 22.12276185039783 26.609595481346744 29.500521728711053 21.767120939544373 32-33 21.91410457309239 26.75846179858902 29.41862121577237 21.908812412546215 34-35 22.346996648964 26.769112687738563 29.22154648247729 21.662344180820146 36-37 22.248616465680993 26.665907790562176 29.23923003365349 21.846245710103336 38-39 22.11066465896337 26.795949179011778 29.33826338990317 21.75512277212168 40-41 22.283472272055842 26.670143740501562 29.32977238369055 21.716611603752046 42-43 22.086107066603553 26.793747424331094 29.073351721695275 22.046793787370085 44-45 22.249921948900994 26.870014465248452 28.868422156260632 22.011641429589922 46-47 22.27267341311737 27.12268892347469 28.54072167762155 22.06391598578639 48-49 22.321301378890738 27.172814670534716 28.44892432676395 22.056959623810595 50-51 22.348494430250657 27.20919411378611 28.303905814187253 22.138405641775982 52-53 22.603450089267767 27.4144567181149 27.938613600386624 22.043479592230707 54-55 22.311482590456006 27.113802087840543 28.247189829465952 22.3275254922375 56-57 22.36766603071983 27.420847251604624 28.1171158457272 22.094370871948353 58-59 22.492947114341288 27.189123844544948 28.147870288146493 22.17005875296727 60-61 22.44029178110825 27.255558765615202 28.495089274421492 21.809060178855056 62-63 22.40181544405554 27.23924959160497 28.34258185541152 22.016353108927973 64-65 22.218054322197382 27.175577244907878 28.34174975469671 22.26461867819803 66-67 22.177880499686463 27.398713372590738 28.137984931654575 22.285421196068224 68-69 22.134844250716604 27.31713421851098 28.2456920481793 22.30232948259312 70-71 22.283124598095355 27.23532207623108 28.228817045682998 22.25273627999057 72-73 22.273106105489067 27.19551437803467 28.18884292734363 22.34253658913263 74-75 22.130783599228344 27.50072892022617 27.90483031136543 22.463657169180053 76-77 22.23196704614897 27.353846502048302 28.007777811801454 22.406408640001278 78-79 22.232732578806594 27.360103899423656 28.01939393778017 22.387769583989584 80-81 22.198823542333805 27.612173038396804 27.874750827358014 22.31425259191138 82-83 22.223017344388122 27.514969561614016 27.954886617428265 22.3071264765696 84-85 22.240879874931228 27.39430846547445 27.81365631449857 22.55115534509575 86-87 22.22717414603168 27.28634649206309 27.926398361893252 22.560081000011984 88-89 22.198150473099183 27.228465566341058 27.9599486627143 22.61343529784546 90-91 22.242900748621093 27.290051561319355 27.93698134938203 22.53006634067752 92-93 22.248140041433007 27.09075585946861 28.006489218364727 22.65461488073365 94-95 22.13937087860516 27.039478851993913 28.047885066255184 22.77326520314574 96-97 22.047873084004895 27.083746610021684 28.07870607673167 22.78967422924175 98-99 21.994496142040543 27.036570566608464 27.848835622502442 23.12009766884855 100-101 21.902513247635497 27.058064571966312 28.046240480992612 22.993181699405582 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 194.0 1 151.0 2 142.0 3 209.5 4 193.5 5 152.5 6 303.0 7 721.5 8 1190.5 9 1484.5 10 1445.0 11 1299.0 12 1351.5 13 1481.0 14 1633.5 15 1798.0 16 1903.0 17 1934.5 18 2076.0 19 2316.0 20 2557.0 21 2867.5 22 3270.5 23 3890.0 24 4800.5 25 5930.5 26 7504.0 27 9675.0 28 11829.5 29 14596.5 30 17828.0 31 20194.0 32 22785.5 33 25966.5 34 29436.5 35 32580.5 36 36333.5 37 40237.0 38 42789.5 39 44552.0 40 46541.0 41 49583.5 42 52349.0 43 54744.0 44 57198.0 45 58886.5 46 59133.5 47 59615.0 48 62493.5 49 62424.5 50 59114.5 51 56385.0 52 53095.5 53 50077.5 54 47659.5 55 44127.0 56 39235.0 57 34348.0 58 30498.5 59 27064.5 60 22933.0 61 18279.5 62 14058.5 63 10858.5 64 8136.0 65 6053.0 66 4516.0 67 3522.0 68 2467.5 69 1508.0 70 1051.0 71 741.0 72 558.0 73 445.5 74 333.5 75 254.0 76 181.0 77 104.0 78 57.5 79 30.5 80 17.0 81 13.0 82 8.0 83 5.0 84 2.0 85 1.5 86 2.0 87 2.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07156066147347064 2 0.0 3 0.0 4 0.0 5 0.00259615423020033 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.3313611436924767E-4 12-13 0.005924557089431522 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.655326576231742E-4 24-25 5.325444574769907E-4 26-27 3.328402859231192E-4 28-29 0.002895710487531137 30-31 2.995562573308073E-4 32-33 0.0 34-35 0.0 36-37 3.328402859231192E-5 38-39 5.325444574769907E-4 40-41 6.656805718462383E-5 42-43 0.01231509057915541 44-45 6.656805718462383E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.328402859231192E-5 82-83 4.6597640029236696E-4 84-85 5.658284860693026E-4 86-87 0.0 88-89 0.0 90-91 0.008454143262447228 92-93 0.0025295861730157063 94-95 0.0 96-97 0.0 98-99 1.9970417155387154E-4 100-101 0.01075074123531675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1502222.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.43268900341675 #Duplication Level Percentage of deduplicated Percentage of total 1 82.51416293317408 53.16609398650465 2 10.583965708375631 13.639067418211893 3 2.955834647082585 5.713571236829891 4 1.2528152121721214 3.228890117785436 5 0.6759596315419506 2.177694835900334 6 0.4133780240558219 1.5981034598901422 7 0.2776201576270914 1.2521469294226144 8 0.20638522138200052 1.0638363827366206 9 0.1592401861851433 0.9234246053982167 >10 0.8705669787374434 10.302834926268376 >50 0.06265934579968509 2.769423593997534 >100 0.026584465980826845 2.995331552035566 >500 3.103079571110635E-4 0.1261619891646415 >1k 4.1374394281475135E-4 0.672597355863477 >5k 1.0343598570368784E-4 0.37082160999052577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5564 0.37038467017524707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4669 0.31080625899500874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3098 0.20622784115796466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.3284028592311924E-5 0.0 20-21 0.0 0.0 0.0 1.331361143692477E-4 0.0 22-23 0.0 0.0 0.0 1.9970417155387154E-4 0.0 24-25 0.0 0.0 0.0 4.659764002923669E-4 0.0 26-27 0.0 0.0 0.0 5.658284860693026E-4 0.0 28-29 0.0 0.0 0.0 0.0011982250293232292 0.0 30-31 0.0 0.0 0.0 0.0028291424303465134 0.0 32-33 0.0 0.0 0.0 0.005125740403216035 0.0 34-35 0.0 0.0 0.0 0.008121302976524108 0.0 36-37 0.0 0.0 0.0 0.011582841950124548 0.0 38-39 0.0 0.0 0.0 0.013946007980178695 0.0 40-41 0.0 0.0 0.0 0.017873523354071504 0.0 42-43 0.0 0.0 0.0 0.023265535986026036 0.0 44-45 0.0 0.0 0.0 0.032318791763134874 0.0 46-47 0.0 0.0 0.0 0.05005917900283713 0.0 48-49 0.0 0.0 0.0 0.08454143262447228 0.0 50-51 0.0 0.0 0.0 0.1125333006706066 0.0 52-53 0.0 0.0 0.0 0.13756289017202517 0.0 54-55 0.0 0.0 0.0 0.17008138610671392 0.0 56-57 0.0 0.0 0.0 0.1987056506961022 0.0 58-59 0.0 0.0 0.0 0.23744825997755326 0.0 60-61 0.0 0.0 0.0 0.29522933361380677 0.0 62-63 0.0 0.0 0.0 0.3438905834157668 0.0 64-65 0.0 0.0 0.0 0.40220420150949726 0.0 66-67 0.0 0.0 0.0 0.4956657537967092 0.0 68-69 0.0 0.0 0.0 0.6000444674621993 0.0 70-71 0.0 0.0 0.0 0.6994305768388427 0.0 72-73 0.0 0.0 0.0 0.8093344392506567 6.656805718462385E-5 74-75 0.0 0.0 0.0 0.9169084196610088 6.656805718462385E-5 76-77 0.0 0.0 0.0 1.0165608012663907 6.656805718462385E-5 78-79 0.0 3.3284028592311924E-5 0.0 1.1355179194553136 6.656805718462385E-5 80-81 0.0 6.656805718462385E-5 0.0 1.3145859932819517 6.656805718462385E-5 82-83 0.0 6.656805718462385E-5 0.0 1.5095971168043074 6.656805718462385E-5 84-85 0.0 6.656805718462385E-5 0.0 1.7049077965839934 6.656805718462385E-5 86-87 0.0 6.656805718462385E-5 0.0 1.9853257374742217 6.656805718462385E-5 88-89 0.0 6.656805718462385E-5 0.0 2.3354404342367507 6.656805718462385E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4185 0.0 37.92449 1 GTATCAA 6365 0.0 30.758673 1 ACCGTAT 130 1.0015681E-5 25.579575 8 ATCAACG 7690 0.0 25.200817 3 TCAACGC 7765 0.0 24.896238 4 TATCAAC 7875 0.0 24.789747 2 CAACGCA 7815 0.0 24.736956 5 GTACATG 5465 0.0 24.520409 1 AACGCAG 8120 0.0 23.807796 6 TACATGG 5640 0.0 23.412432 2 ACATGGG 5635 0.0 22.758871 3 CATGGGG 3075 0.0 21.470913 4 ACGCAGA 9130 0.0 21.124863 7 TACCTGG 1315 0.0 20.949955 2 CGCAGAG 9205 0.0 20.79792 8 GAGTACT 5090 0.0 20.672577 12-13 GTACCTG 1355 0.0 19.638916 1 GTACTTT 5355 0.0 19.513897 14-15 AGTACTT 5355 0.0 19.250362 12-13 GCAGAGT 9735 0.0 19.177645 9 >>END_MODULE