FastQCFastQC Report
Thu 26 May 2016
SRR937251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006545
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47340.23592792586261457No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41440.20652414972004118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.16461130948969496No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32350.1612223996969916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.11711673548313146No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23050.11487407459090128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA804.495274E-429.6869876
GCGTTAG700.00836921627.1586721
GTATCAA103900.024.3356341
GTACATG64950.024.2943631
TACATGG65400.023.5393472
ACATGGG67350.022.2212053
GAGTACT47700.021.90628212-13
TCAACGC115150.021.579594
ATCAACG116350.021.3156573
CAACGCA117150.021.172215
TATCAAC118400.021.1086862
AACGCAG119550.020.7399166
GTACTTT52300.020.11450214-15
AGTACTT51200.019.8521812-13
AGAGTAC84400.019.58496910-11
GTACACG2451.7468847E-719.3990521
CATGGGG37000.019.0048944
CGTATAC1759.923731E-519.004423
ACGCAGA131400.018.8347997
CGCAGAG131550.018.7411028
Produced by FastQC (version 0.11.5)