FastQCFastQC Report
Thu 26 May 2016
SRR937251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006545
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58010.2891039074628279No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45550.22700711920241012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.22272114505281468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.16196995332773498No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20820.10376044394718284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63400.021.5094871
GTATCAA117050.021.0684871
CGCGGAA1601.8189894E-1120.78036382-83
GGACCGA4350.020.746246
TACATGG64300.020.46182
ATTCGCG1652.7284841E-1120.15065280-81
AGTACTT64150.019.99384112-13
ACATGGG63900.019.7722383
GAGTACT59550.019.66365812-13
AGAGTAC97600.019.17677510-11
AGGACCG4600.018.5861635
GTGTAAG8250.018.4303441
GTACTTT63300.018.4214114-15
ACCGAGT5750.018.17548
TCAACGC134850.018.0340844
ATCAACG135100.018.0007113
CAACGCA135400.017.9608275
TATCAAC137200.017.7944282
AACGCAG138800.017.5550866
TACTTTT74000.017.20196714-15
Produced by FastQC (version 0.11.5)