FastQCFastQC Report
Thu 26 May 2016
SRR937250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973481
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47510.24074212014202315No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41180.20866681766888054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.167470576103849No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26460.13407780465076685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.12014303659371435No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22900.11603861400236436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67050.026.1013431
TACATGG66550.025.787392
ACATGGG67150.024.3516033
GTATCAA105100.022.5341131
ATAACGC1703.1306954E-622.3694973
TATAACG1503.0155576E-522.1847762
GAGTACT47200.020.3777512-13
AGAGTAC83450.020.34831210-11
GTATAGG4800.019.8153881
TCAACGC120000.019.3314654
CAACGCA120250.019.2517435
AGTACTT51950.019.24596612-13
ATCAACG120900.019.1870693
TATCAAC122250.019.1710682
GTACTTT50350.019.10214814-15
ACCGCGC2001.2854531E-519.000078
AACGCAG123300.018.7222236
CATGGGA35050.018.3093074
CATGGGG38100.018.2160434
ACGCAGA135650.016.9130747
Produced by FastQC (version 0.11.5)