FastQCFastQC Report
Thu 26 May 2016
SRR937250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973481
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58990.2989134427947368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45950.23283730626238613No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45670.23141849351475896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31410.1591603871534613No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20240.10255989289990632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG64150.025.5576341
TACATGG66650.024.5158752
ACATGGG68250.022.8276023
GTATCAA113850.022.1228491
AGAGTAC93200.020.84685510-11
AGTACTT59250.020.6052512-13
GAGTACT54800.020.41472212-13
GTACTTT59350.018.84678514-15
CAACGCA132150.018.798525
TCAACGC132650.018.7634684
TATCAAC133750.018.6446672
ATCAACG133050.018.6356563
AACGCAG134650.018.4485576
CATGGGG39100.018.34384
TAACGCG650.007568971318.26811676-77
GTATAGG6750.017.6008821
CATGGGA33800.017.1447934
TACTTTT70850.017.12845214-15
GGTATCA88600.017.0029321
ACCGTGC4255.638867E-1116.7662328
Produced by FastQC (version 0.11.5)