##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937250_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1973481 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20766250093109 33.0 31.0 34.0 31.0 34.0 2 32.4391686568049 34.0 31.0 34.0 31.0 34.0 3 32.530563000099825 34.0 31.0 34.0 31.0 34.0 4 36.12940585696037 37.0 35.0 37.0 35.0 37.0 5 35.97967601410908 37.0 35.0 37.0 35.0 37.0 6 36.064667458161495 37.0 35.0 37.0 35.0 37.0 7 36.02895999505442 37.0 35.0 37.0 35.0 37.0 8 36.00311429398104 37.0 35.0 37.0 35.0 37.0 9 37.72165883532702 39.0 37.0 39.0 35.0 39.0 10-11 37.63861065802001 39.0 37.0 39.0 35.0 39.0 12-13 37.55564760947787 39.0 37.0 39.0 35.0 39.0 14-15 38.81232198333807 40.0 38.0 41.0 35.0 41.0 16-17 38.79095339656171 40.0 38.0 41.0 35.0 41.0 18-19 38.770737341783374 40.0 38.0 41.0 35.0 41.0 20-21 38.668846571109626 40.0 38.0 41.0 34.0 41.0 22-23 38.54396748689245 40.0 38.0 41.0 34.0 41.0 24-25 38.39715913150418 40.0 38.0 41.0 34.0 41.0 26-27 38.190291165711756 40.0 38.0 41.0 34.0 41.0 28-29 38.19342091461737 40.0 38.0 41.0 34.0 41.0 30-31 38.36365158823419 40.0 38.0 41.0 34.0 41.0 32-33 38.35943898117084 40.0 38.0 41.0 34.0 41.0 34-35 38.26622678404301 40.0 38.0 41.0 34.0 41.0 36-37 38.13306639384925 40.0 38.0 41.0 34.0 41.0 38-39 38.00995525165938 40.0 38.0 41.0 33.0 41.0 40-41 37.88095983695814 40.0 37.0 41.0 33.0 41.0 42-43 37.69957146787833 40.0 37.0 41.0 33.0 41.0 44-45 37.46759127653117 40.0 37.0 41.0 32.5 41.0 46-47 37.20215902762682 40.0 37.0 41.0 32.0 41.0 48-49 36.945730412403265 40.0 36.5 41.0 31.0 41.0 50-51 36.761002006099886 39.0 36.0 41.0 31.0 41.0 52-53 36.544630021773706 39.0 36.0 41.0 30.5 41.0 54-55 36.35329197494174 39.0 35.5 40.5 30.0 41.0 56-57 36.11060405446011 39.0 35.0 40.0 30.0 41.0 58-59 35.85247261057999 38.5 35.0 40.0 29.0 41.0 60-61 35.51331302404229 38.0 35.0 40.0 28.5 41.0 62-63 35.154191248864315 38.0 34.0 40.0 28.0 41.0 64-65 34.81251200290249 37.0 34.0 40.0 28.0 41.0 66-67 34.42061996036445 36.5 34.0 39.0 27.5 41.0 68-69 33.97832864871767 36.0 33.0 39.0 26.0 40.0 70-71 33.59183746891914 35.5 33.0 38.5 26.0 40.0 72-73 33.155234076233825 35.0 33.0 38.0 26.0 40.0 74-75 32.71697219279031 35.0 32.0 37.0 25.5 39.0 76-77 31.394224976070202 34.0 30.5 36.0 24.0 38.5 78-79 32.02938639895697 35.0 32.0 36.0 25.0 38.5 80-81 31.863648041202325 35.0 32.0 36.0 25.0 37.5 82-83 31.543186886521838 34.5 32.0 35.5 24.0 37.0 84-85 31.22261273354038 34.0 31.0 35.0 23.5 36.5 86-87 30.907375850084193 34.0 31.0 35.0 22.0 36.0 88-89 30.557103666060122 34.0 31.0 35.0 20.0 36.0 90-91 30.129564966675634 34.0 30.5 35.0 18.5 35.5 92-93 29.73972057496373 34.0 30.0 35.0 16.0 35.0 94-95 29.354251447062325 34.0 30.0 35.0 7.0 35.0 96-97 28.94756042748828 34.0 29.0 35.0 2.0 35.0 98-99 28.32236160368405 34.0 29.0 35.0 2.0 35.0 100-101 26.70485831887918 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09101763255943496 1101 2 0.18350414614083377 1101 3 0.1602289965383079 1101 4 0.07677977420807736 1101 5 0.006591549115455564 1101 6 0.08231434890234368 1101 7 0.07288839731871377 1101 8 0.08339548589585632 1101 9 0.16023547266916438 1101 10-11 0.0786996321381892 1101 12-13 0.2001623288534944 1101 14-15 0.17914395762998225 1101 16-17 0.22502306018698448 1101 18-19 0.23973438478109443 1101 20-21 0.1324669272194754 1101 22-23 0.1104754129191221 1101 24-25 0.2727204543688586 1101 26-27 0.13624364422457802 1101 28-29 0.11688129974098871 1101 30-31 0.2294636046198164 1101 32-33 0.17529784917478253 1101 34-35 0.1575234074327554 1101 36-37 0.1868397488462037 1101 38-39 0.07596288983583577 1101 40-41 0.05497385842465974 1101 42-43 0.12373523355835658 1101 44-45 0.1558751148498203 1101 46-47 0.11161611199820243 1101 48-49 0.13294421632090092 1101 50-51 0.08496353734709317 1101 52-53 0.1979379339327565 1101 54-55 0.06285490912409131 1101 56-57 -0.03717326061426718 1101 58-59 0.039077846348597234 1101 60-61 0.03328177649416375 1101 62-63 0.010510253733421848 1101 64-65 0.03739181176910478 1101 66-67 -0.022146758852535697 1101 68-69 -0.07692325518703313 1101 70-71 -0.21014142069294905 1101 72-73 -0.2708760968823114 1101 74-75 -0.22194363035293918 1101 76-77 -0.13471681206008768 1101 78-79 -0.11402039266855368 1101 80-81 -0.18644107701634738 1101 82-83 -0.0649248287287243 1101 84-85 -0.15836644965409974 1101 86-87 -0.33016572566009117 1101 88-89 -0.19145999828153748 1101 90-91 -0.07976115114279025 1101 92-93 -0.08955899412385904 1101 94-95 -0.23770302331325155 1101 96-97 -0.17706947873989876 1101 98-99 -0.3710813652830751 1101 100-101 0.005225393490391639 1102 1 0.1630581073007633 1102 2 0.06002012962809289 1102 3 0.07447289026399773 1102 4 0.05600421782924059 1102 5 -9.232038549882304E-4 1102 6 0.10487183917729226 1102 7 0.13357173109309173 1102 8 0.1644766206175987 1102 9 0.1506850744051178 1102 10-11 0.14929160334674663 1102 12-13 0.11049106208274395 1102 14-15 0.1632066147524185 1102 16-17 0.1886672423563951 1102 18-19 0.15209992104826142 1102 20-21 0.17776962774079408 1102 22-23 0.21043042628279807 1102 24-25 0.1590589060346801 1102 26-27 0.21359138430767644 1102 28-29 0.13264795802779616 1102 30-31 0.14075061935955802 1102 32-33 0.11637891773970921 1102 34-35 0.14558805587541457 1102 36-37 0.21434163431552378 1102 38-39 0.19544392267608401 1102 40-41 0.14966940474765522 1102 42-43 0.2801701418650069 1102 44-45 0.12710268768847754 1102 46-47 -0.020310341467094872 1102 48-49 0.2282568403649421 1102 50-51 0.2192630358784129 1102 52-53 0.20205437654833958 1102 54-55 0.2924802630630765 1102 56-57 0.36258427464731824 1102 58-59 0.27356274936270353 1102 60-61 0.11776998734917044 1102 62-63 0.3103059945574742 1102 64-65 0.32532116951870904 1102 66-67 0.25253486146025494 1102 68-69 -0.016921667423396514 1102 70-71 0.021580112028210863 1102 72-73 0.1110900506738588 1102 74-75 0.08369408829534564 1102 76-77 0.12087625073596442 1102 78-79 0.14302679698980825 1102 80-81 0.15788581043767635 1102 82-83 0.18947625359681908 1102 84-85 0.26546588250605296 1102 86-87 0.2820292914083673 1102 88-89 0.3843092401211692 1102 90-91 0.3112646764790057 1102 92-93 0.2758328857882866 1102 94-95 0.20261856497963748 1102 96-97 0.36177813564524186 1102 98-99 0.5427062117896142 1102 100-101 0.6073520044089129 1103 1 0.15334445224512905 1103 2 0.10678009734017024 1103 3 0.1287231032380589 1103 4 0.09513688752487326 1103 5 0.06568739278791469 1103 6 0.05976472111078124 1103 7 0.07509063056933485 1103 8 0.10582362898932018 1103 9 0.030432949923095975 1103 10-11 0.12165739681974941 1103 12-13 0.1553086274473685 1103 14-15 0.1804764121378355 1103 16-17 0.10825687945910545 1103 18-19 0.07501793523601918 1103 20-21 0.07475394128068302 1103 22-23 0.1376517983616381 1103 24-25 0.08023828935645128 1103 26-27 0.11834662189207279 1103 28-29 0.1251991243894608 1103 30-31 0.22937313729143938 1103 32-33 0.25416985082903665 1103 34-35 0.28815693720942903 1103 36-37 0.37250067729947745 1103 38-39 0.36447483152317517 1103 40-41 0.32844108340398037 1103 42-43 0.331580043318489 1103 44-45 0.29731432080515674 1103 46-47 0.1767125243224399 1103 48-49 0.10416526677582993 1103 50-51 0.1824265749814984 1103 52-53 0.37179158364326526 1103 54-55 0.3932480542853867 1103 56-57 0.3215555441830844 1103 58-59 0.18840451094744282 1103 60-61 0.21742673100202126 1103 62-63 0.33032259864657476 1103 64-65 0.34375295734395195 1103 66-67 0.2801485265072614 1103 68-69 0.14582798550775777 1103 70-71 0.10168136673467387 1103 72-73 0.2760777389324076 1103 74-75 0.27357952928312557 1103 76-77 -0.006456111067532078 1103 78-79 0.32887016771191924 1103 80-81 0.2982725292698376 1103 82-83 0.3887946708577097 1103 84-85 0.30742785348899204 1103 86-87 0.41476422677994407 1103 88-89 0.46755054762417103 1103 90-91 0.5656702487744951 1103 92-93 0.5809499492177466 1103 94-95 0.4547662177951004 1103 96-97 0.37455247121874535 1103 98-99 0.21115472859616347 1103 100-101 0.5882653542017238 1104 1 0.0359182356560126 1104 2 0.13864915539000577 1104 3 0.11392708081978498 1104 4 0.05238065351274912 1104 5 0.029009383542643263 1104 6 0.08332732308068813 1104 7 0.010358622593024336 1104 8 0.08645721460376166 1104 9 0.040951586497307346 1104 10-11 0.055714810913251256 1104 12-13 0.03173589143109723 1104 14-15 0.0636074107379514 1104 16-17 0.010456250770566555 1104 18-19 0.0602192376812809 1104 20-21 -0.006486491172431386 1104 22-23 -0.07017735042882123 1104 24-25 0.02643358438938037 1104 26-27 -0.05889784907294171 1104 28-29 -0.08561170003095242 1104 30-31 -0.06003225225877884 1104 32-33 -0.1112531606318683 1104 34-35 -0.09191087277908849 1104 36-37 0.015027069881107025 1104 38-39 0.005222100995659673 1104 40-41 -0.025154362947262143 1104 42-43 -7.609717478800349E-4 1104 44-45 0.17352851803761382 1104 46-47 0.05735753434318269 1104 48-49 -0.0849939472753718 1104 50-51 0.015238256704463993 1104 52-53 0.14820194705897904 1104 54-55 0.01981300911649697 1104 56-57 -0.12889683712897693 1104 58-59 -0.033524337975954666 1104 60-61 -0.20259550095560286 1104 62-63 -0.14788511027843754 1104 64-65 -0.0629442625283474 1104 66-67 -0.21662053808878312 1104 68-69 -0.24711305322570354 1104 70-71 -0.13727126383716381 1104 72-73 -0.14752115047681258 1104 74-75 -0.17663964307371316 1104 76-77 -0.09226898118052773 1104 78-79 -0.16409847213539663 1104 80-81 -0.07605270689749943 1104 82-83 -0.0604095232527051 1104 84-85 -0.19533398886804676 1104 86-87 -0.09028501461429883 1104 88-89 0.04355967204256217 1104 90-91 0.11698833571831813 1104 92-93 0.07366177596458101 1104 94-95 -0.08732957223249471 1104 96-97 -0.19462167953557952 1104 98-99 0.11382744170068193 1104 100-101 0.5149138991792626 1105 1 0.02526348435785053 1105 2 0.09793911470725902 1105 3 0.03165149473474571 1105 4 -0.009790101088221093 1105 5 -0.05780348334872798 1105 6 -0.04405378466459098 1105 7 0.010885666204622169 1105 8 -0.018253488648099392 1105 9 -0.03184944951985358 1105 10-11 -0.04466942897692405 1105 12-13 -0.062190809351193366 1105 14-15 0.018196956702432487 1105 16-17 0.0722983874053682 1105 18-19 0.008025763736057456 1105 20-21 0.05446076933016997 1105 22-23 0.03928866655202512 1105 24-25 0.022060220420591747 1105 26-27 0.01832972584882242 1105 28-29 -0.1297672883245582 1105 30-31 -0.07698156697659897 1105 32-33 -0.05022260841565185 1105 34-35 -0.061589238140776104 1105 36-37 0.02534394857824651 1105 38-39 0.10199671957921197 1105 40-41 0.008592584591823993 1105 42-43 -0.15724680807824853 1105 44-45 0.007232298195177123 1105 46-47 -0.019685418456290904 1105 48-49 -0.005633414308796603 1105 50-51 -0.17685690478420923 1105 52-53 -0.13536555467901223 1105 54-55 -0.004939845075632832 1105 56-57 -0.03853881765848399 1105 58-59 0.010654935908526397 1105 60-61 -0.014666023155427865 1105 62-63 0.006767210344776231 1105 64-65 0.04758195914619989 1105 66-67 -0.08423000630178024 1105 68-69 -0.04138785376478893 1105 70-71 -0.09094632639222056 1105 72-73 -0.008178348763173915 1105 74-75 -0.022866669619141078 1105 76-77 -0.00883499715748215 1105 78-79 -0.0035242832178639105 1105 80-81 -0.019700764741791943 1105 82-83 -0.054650944363064724 1105 84-85 -0.11815723600883743 1105 86-87 -0.0714725379861747 1105 88-89 -0.005922664467252758 1105 90-91 -0.06947953720895583 1105 92-93 -0.09839066989423984 1105 94-95 -0.10437441360491562 1105 96-97 -0.08281125963240399 1105 98-99 -0.012698004466834334 1105 100-101 -0.1846102243597585 1106 1 -0.12948225859850737 1106 2 -0.02537787587247209 1106 3 -0.0888320706580572 1106 4 0.01421889068268456 1106 5 -0.11252621798975326 1106 6 -0.044822356720708 1106 7 -0.08896705362753465 1106 8 -0.04020453315150263 1106 9 -0.10113648145390641 1106 10-11 -0.14764898896900291 1106 12-13 -0.22141407827022164 1106 14-15 0.006550302441617362 1106 16-17 -0.026285085280427722 1106 18-19 -0.04394296314615076 1106 20-21 -0.2673610513114255 1106 22-23 -0.13677564488961025 1106 24-25 -0.10598400203309666 1106 26-27 -0.04143361526848821 1106 28-29 -0.12108476534655921 1106 30-31 -0.15039687346891384 1106 32-33 -0.20179072785138175 1106 34-35 -0.304786289033963 1106 36-37 -0.3242224305799226 1106 38-39 -0.31587370082279875 1106 40-41 -0.3177482814101751 1106 42-43 -0.4206972401494866 1106 44-45 -0.4594774823937513 1106 46-47 -0.3918473612878728 1106 48-49 -0.5871049156636943 1106 50-51 -0.41957526144476276 1106 52-53 -0.3012796787527847 1106 54-55 -0.33327490543624805 1106 56-57 -0.425121213703342 1106 58-59 -0.4761559599941734 1106 60-61 -0.3876169187616867 1106 62-63 -0.4189215737247096 1106 64-65 -0.3986014060102434 1106 66-67 -0.35315594580237786 1106 68-69 -0.3377992976898554 1106 70-71 -0.24904259210558877 1106 72-73 -0.2533583930415446 1106 74-75 -0.4208974711170086 1106 76-77 -0.3319732269387963 1106 78-79 -0.37505417112054573 1106 80-81 -0.5044140467718599 1106 82-83 -0.6938984141221205 1106 84-85 -0.47954847438845505 1106 86-87 -0.27644689870535544 1106 88-89 -0.304612285325252 1106 90-91 -0.3606855603345025 1106 92-93 -0.27263958146280487 1106 94-95 -0.5924966655766255 1106 96-97 -0.5435017381649381 1106 98-99 -0.5519249082280702 1106 100-101 -0.5449560666258009 1107 1 -0.12553338019375104 1107 2 -0.0404686084114374 1107 3 0.015394208295795409 1107 4 0.001033849090440242 1107 5 -0.0955720599686316 1107 6 -0.04393710032359621 1107 7 -0.03966786674730116 1107 8 -0.08475393782370588 1107 9 -0.1042329171555636 1107 10-11 -0.09971201622844461 1107 12-13 -0.1043089594231219 1107 14-15 -0.059055507008906716 1107 16-17 -0.07344246528529652 1107 18-19 -0.03111102543509503 1107 20-21 -0.055391543079913674 1107 22-23 -0.09118181957082072 1107 24-25 -0.0027531015201205378 1107 26-27 -0.03556763245823191 1107 28-29 -0.1019096195797502 1107 30-31 -0.03150447831000491 1107 32-33 0.024123976161071425 1107 34-35 -0.14912427721568378 1107 36-37 -0.1245797253405172 1107 38-39 -0.09897019002503527 1107 40-41 -0.0904022583318067 1107 42-43 -0.030632477687639437 1107 44-45 -0.12951626140333872 1107 46-47 -0.03913109184821906 1107 48-49 -0.047229946537989065 1107 50-51 0.03296062184548987 1107 52-53 0.07574048116047294 1107 54-55 0.05522942320716595 1107 56-57 -0.07674821117009145 1107 58-59 0.09491225949734172 1107 60-61 0.10228368606247074 1107 62-63 0.024531514730490755 1107 64-65 -0.0219079563547524 1107 66-67 0.0530821335978473 1107 68-69 -0.08888192667308914 1107 70-71 -0.12329186739553677 1107 72-73 -0.009264813379616044 1107 74-75 1.106047967667223E-4 1107 76-77 0.09012590430081957 1107 78-79 0.12891548334590652 1107 80-81 -0.034398094679851 1107 82-83 0.05411203275937382 1107 84-85 -0.15274004305245015 1107 86-87 0.05917905371657284 1107 88-89 0.04503875708912375 1107 90-91 0.1744109146009194 1107 92-93 0.18922305934405514 1107 94-95 -0.028793693708124124 1107 96-97 0.15842035962878853 1107 98-99 0.14524927987821457 1107 100-101 0.4070147612868844 1108 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2316 56-57 -0.6210413775845964 2316 58-59 -0.5358809758392695 2316 60-61 -0.597332343060863 2316 62-63 -0.4894012139705026 2316 64-65 -0.5415381352698958 2316 66-67 -0.372158637067578 2316 68-69 -0.5234288426993814 2316 70-71 -0.473484932336774 2316 72-73 -0.45290214026471887 2316 74-75 -0.5264039792873803 2316 76-77 -0.5292289183368517 2316 78-79 -0.41822258839619764 2316 80-81 -0.48708962755735996 2316 82-83 -0.5366467581309458 2316 84-85 -0.5592381522616066 2316 86-87 -0.371706852791835 2316 88-89 -0.5581685290572018 2316 90-91 -0.6795552620821503 2316 92-93 -0.604657155692923 2316 94-95 -0.4861905305985559 2316 96-97 -0.36253212729677387 2316 98-99 -0.5546721655263411 2316 100-101 -0.8487938777256865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 11.0 5 52.0 6 56.0 7 140.0 8 354.0 9 773.0 10 1175.0 11 2092.0 12 3069.0 13 4192.0 14 5562.0 15 7555.0 16 10055.0 17 14048.0 18 12623.0 19 7926.0 20 7219.0 21 7253.0 22 8050.0 23 8874.0 24 10333.0 25 12468.0 26 15611.0 27 19330.0 28 24895.0 29 31786.0 30 40984.0 31 52299.0 32 67389.0 33 90084.0 34 125217.0 35 182407.0 36 292175.0 37 470129.0 38 398977.0 39 38309.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.542703000844575 19.484552702098473 15.161326953864435 22.811417343192513 2 15.838460061181234 20.43252506611414 39.32827323901269 24.40074163369194 3 19.770699591229913 23.716316498613363 29.79582777842807 26.717156131728657 4 13.282519568214743 16.640393294893645 37.028732478295964 33.04835465859565 5 14.051498274437863 37.16283123951613 34.308736260116454 14.47693422592955 6 32.910780493959656 36.169945390910776 16.884986478207797 14.034287636921764 7 29.25612154360746 32.199853963630765 20.340454253169906 18.20357023959187 8 26.65802204328291 35.01052201668017 19.29509328947175 19.03636265056517 9 26.05609073510209 16.951467989810897 20.022893557120643 36.96954771796638 10-11 25.07427231374409 26.51728088590668 27.37913362226442 21.029313178084816 12-13 25.9886574771438 23.609808700393565 27.96053756757791 22.440996254884727 14-15 22.765179902922807 25.338095476976974 26.075498066614273 25.82122655348595 16-17 22.34300710267796 28.641066217511092 26.61753520809169 22.39839147171926 18-19 22.2439689057052 27.46902554420336 28.730248733076223 21.556756817015213 20-21 22.90799860753663 26.264301505816373 28.931466783820063 21.896233102826933 22-23 22.6075187291752 26.132041793632855 28.87129372114995 22.389145756041994 24-25 22.70661692013716 26.40464440571967 28.850107247585598 22.038631426557572 26-27 22.52933071793814 26.510266574712315 28.92224401718215 22.038158690167386 28-29 22.22083152599127 26.955653906493865 28.853540431086554 21.969974136428313 30-31 22.459456371181485 26.36538709540931 28.889785227522925 22.285371305886283 32-33 22.42571498064333 26.530291667511197 28.753166994345015 22.290826357500457 34-35 22.867385092635807 26.49093150630789 28.600376694784497 22.04130670627181 36-37 22.74286588739868 26.306197795159402 28.863994292304547 22.086942025137372 38-39 22.414629629113524 26.65940198365877 28.822338469536852 22.103629917690853 40-41 22.66062421189366 26.582933046462838 28.670792541400843 22.085650200242668 42-43 22.466961915172558 26.580195241056266 28.445338737607393 22.507504106163783 44-45 22.729380216987142 26.473500378265612 28.331841046354135 22.465278358393114 46-47 22.82958386728831 26.864940680959176 28.02243852360372 22.28303692814879 48-49 22.774407955893157 26.984431819822895 27.875699810563116 22.365460413720832 50-51 22.661251869159116 26.968564683419803 28.077189494096977 22.2929939533241 52-53 22.903143587108648 27.149276556414613 27.51464673029038 22.43293312618636 54-55 22.653853774117916 26.909379923090214 27.759831485583085 22.676934817208778 56-57 22.829254500043326 27.06499327837461 27.688941520085574 22.41681070149649 58-59 23.03095393368368 26.850600537831376 27.65400325617526 22.46444227230969 60-61 22.78058415561133 26.882017105814548 28.272124231244185 22.065274507329942 62-63 22.624590761198107 26.99747299315271 28.06317871821416 22.314757527435024 64-65 22.605082085918234 27.109635208041023 27.831228169919044 22.454054536121706 66-67 22.560490828135666 27.08701021190475 27.728237566006463 22.62426139395312 68-69 22.525932603354175 26.89795341328343 27.855094627209485 22.72101935615291 70-71 22.676858809383013 26.918779557543242 27.814861151437487 22.589500481636257 72-73 22.626668308435704 26.86192570387047 27.843668117402704 22.667737870291123 74-75 22.480251900068964 27.358611509307668 27.43439131159611 22.72674527902726 76-77 22.703790915646007 26.882853191897972 27.600392403068486 22.812963489387535 78-79 22.63733473998483 26.973707879629956 27.66066660890072 22.7282907714845 80-81 22.65907297815383 27.064435887652326 27.598770902785486 22.67772023140836 82-83 22.538066744718517 26.941366610006867 27.77180551357517 22.748761131699446 84-85 22.460754606076573 27.004023349615906 27.733749518616857 22.80147252569066 86-87 22.524311103071174 26.949689406687977 27.663808265699036 22.86219122454181 88-89 22.462567412607466 26.979129771201244 27.359751626694152 23.198551189497138 90-91 22.51770488895646 26.726312188818362 27.704002128387366 23.051980793837814 92-93 22.510762404667428 26.598157868533338 27.88202007630913 23.0090596504901 94-95 22.249644156695705 26.697368761087642 27.880101201886415 23.172885880330238 96-97 22.12643547112944 26.7775570172705 27.98007682871028 23.115930682889775 98-99 22.183108494145397 26.834613031256215 27.532326043636836 23.449952430961556 100-101 21.765434297921786 26.732419126830504 27.87114416527578 23.631002409971927 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 90.0 2 87.5 3 137.0 4 139.0 5 126.5 6 238.5 7 572.0 8 1040.0 9 1340.0 10 1362.5 11 1342.5 12 1460.0 13 1591.5 14 1685.5 15 1832.0 16 2011.0 17 2173.0 18 2274.5 19 2371.0 20 2601.5 21 3012.0 22 3556.5 23 4334.5 24 5304.5 25 6688.0 26 8568.0 27 10917.5 28 13377.5 29 16294.0 30 19792.5 31 23526.5 32 27722.5 33 31528.5 34 35569.0 35 39660.5 36 44675.5 37 50243.0 38 54076.5 39 56725.5 40 58760.5 41 62373.5 42 66011.0 43 69211.0 44 73429.0 45 76407.5 46 78339.0 47 83771.5 48 91072.0 49 88407.5 50 80551.5 51 75609.5 52 71903.5 53 69803.5 54 67334.5 55 62030.5 56 54804.5 57 48499.0 58 43499.0 59 38366.0 60 32104.0 61 25714.0 62 20084.0 63 15202.5 64 11173.0 65 8257.0 66 5987.0 67 4426.0 68 3102.5 69 1999.0 70 1402.0 71 1045.5 72 809.0 73 598.5 74 455.5 75 330.5 76 222.0 77 123.0 78 56.5 79 34.5 80 25.5 81 16.0 82 6.0 83 3.5 84 3.0 85 2.0 86 2.5 87 1.0 88 0.0 89 1.0 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04575671111097599 2 0.0 3 0.0 4 0.0 5 0.010691767491047546 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005599243164742909 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011401173864861126 24-25 5.067188384382723E-4 26-27 0.0019255315860654348 28-29 0.002482922308347534 30-31 0.0010387736187984582 32-33 5.067188384382723E-5 34-35 0.0 36-37 2.5335941921913617E-4 38-39 3.2936724498487695E-4 40-41 2.5335941921913616E-5 42-43 0.011553189516392608 44-45 0.0 46-47 0.0 48-49 2.5335941921913616E-5 50-51 0.0 52-53 1.5201565153148168E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.2936724498487695E-4 84-85 5.067188384382723E-5 86-87 0.0 88-89 0.0 90-91 0.007904813879637047 92-93 0.003065648972551547 94-95 0.0 96-97 0.0 98-99 0.001697508108768212 100-101 0.011223822271407731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1973481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.857426972112194 #Duplication Level Percentage of deduplicated Percentage of total 1 76.07911002672284 43.25662442447682 2 13.502938410965715 15.354846692208234 3 4.443328568086855 7.579086887192946 4 2.0029737733350403 4.555357401778122 5 1.0974630818355737 3.119946351502766 6 0.666959701150229 2.2752967500894545 7 0.41521235062315676 1.6525534132432622 8 0.3077305013360922 1.3997411605446666 9 0.23172769805702934 1.185789660272389 >10 1.1476251500763808 11.778479670000136 >50 0.0688088518965493 2.6749914271265762 >100 0.03407140819815453 3.2723255456976874 >500 9.80663255716183E-4 0.3814860259740668 >1k 9.806632557161786E-4 1.2144596992177297 >5k 8.915120506510713E-5 0.2990148906751278 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5899 0.2989134427947368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4595 0.23283730626238613 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4567 0.23141849351475896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3141 0.1591603871534613 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2024 0.10255989289990632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.0671883843827224E-5 0.0 0.0 0.0 0.0 6 5.0671883843827224E-5 0.0 0.0 0.0 0.0 7 1.0134376768765445E-4 0.0 0.0 0.0 0.0 8 1.0134376768765445E-4 0.0 0.0 0.0 0.0 9 1.0134376768765445E-4 0.0 0.0 5.0671883843827224E-5 0.0 10-11 1.0134376768765445E-4 0.0 0.0 5.0671883843827224E-5 0.0 12-13 2.026875353753089E-4 0.0 0.0 5.0671883843827224E-5 0.0 14-15 2.026875353753089E-4 0.0 0.0 5.0671883843827224E-5 2.5335941921913612E-5 16-17 2.2802347729722252E-4 0.0 0.0 7.600782576574084E-5 5.0671883843827224E-5 18-19 3.2936724498487695E-4 0.0 0.0 1.0134376768765445E-4 5.0671883843827224E-5 20-21 4.053750707506178E-4 0.0 0.0 1.0134376768765445E-4 5.0671883843827224E-5 22-23 4.5604695459444504E-4 0.0 0.0 1.0134376768765445E-4 7.600782576574084E-5 24-25 5.067188384382722E-4 0.0 0.0 1.5201565153148168E-4 1.0134376768765445E-4 26-27 5.067188384382722E-4 0.0 0.0 2.2802347729722252E-4 1.0134376768765445E-4 28-29 5.573907222820994E-4 0.0 0.0 8.107501415012356E-4 1.0134376768765445E-4 30-31 6.587344899697539E-4 0.0 0.0 0.0017228440506901258 1.0134376768765445E-4 32-33 6.840704318916676E-4 0.0 0.0 0.0032176646240830286 1.0134376768765445E-4 34-35 7.347423157354949E-4 0.0 0.0 0.005371219687445686 1.0134376768765445E-4 36-37 7.600782576574084E-4 0.0 0.0 0.007778134170027479 1.0134376768765445E-4 38-39 9.120939091888901E-4 0.0 0.0 0.011755877051767918 1.5201565153148168E-4 40-41 9.374298511108036E-4 0.0 0.0 0.020750136434047252 1.5201565153148168E-4 42-43 9.627657930327173E-4 0.0 0.0 0.028224239301011766 1.5201565153148168E-4 44-45 9.627657930327173E-4 0.0 0.0 0.03787723317326085 1.5201565153148168E-4 46-47 0.0010641095607203718 0.0 0.0 0.055409704983225075 1.5201565153148168E-4 48-49 0.0012161252122518534 0.0 0.0 0.06911644956298034 1.5201565153148168E-4 50-51 0.0012161252122518534 0.0 0.0 0.0854327961606927 1.5201565153148168E-4 52-53 0.0012161252122518534 0.0 0.0 0.1036746743444705 1.5201565153148168E-4 54-55 0.0012161252122518534 0.0 0.0 0.12427279512698627 1.5201565153148168E-4 56-57 0.0012161252122518534 0.0 0.0 0.18811936877020857 1.7735159345339527E-4 58-59 0.0012161252122518534 0.0 0.0 0.2434784018695898 2.026875353753089E-4 60-61 0.0012414611541737672 0.0 0.0 0.28647349531107724 2.026875353753089E-4 62-63 0.0012667970960956807 0.0 0.0 0.3499146938835489 2.026875353753089E-4 64-65 0.0012667970960956807 0.0 0.0 0.42197011270947127 2.026875353753089E-4 66-67 0.0012667970960956807 0.0 0.0 0.5374260000476315 2.2802347729722252E-4 68-69 0.0012667970960956807 0.0 0.0 0.6735053441102297 2.533594192191361E-4 70-71 0.0012667970960956807 0.0 0.0 0.7444459814915878 2.533594192191361E-4 72-73 0.0012667970960956807 0.0 0.0 0.8288906759173258 2.786953611410497E-4 74-75 0.0012667970960956807 0.0 0.0 0.9938529937709053 3.0403130306296336E-4 76-77 0.0012667970960956807 0.0 0.0 1.1962364978431512 3.547031869067906E-4 78-79 0.0012667970960956807 0.0 0.0 1.391931313248012 3.547031869067906E-4 80-81 0.0013934768057052489 0.0 0.0 1.5499262470730653 3.547031869067906E-4 82-83 0.0014188127476271624 0.0 0.0 1.7715904029478875 3.547031869067906E-4 84-85 0.0014188127476271624 0.0 0.0 2.1428886318135314 3.547031869067906E-4 86-87 0.0014188127476271624 0.0 0.0 2.4979718578491505 3.800391288287042E-4 88-89 0.0014188127476271624 0.0 0.0 2.9300003395016216 4.053750707506178E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6415 0.0 25.557634 1 TACATGG 6665 0.0 24.515875 2 ACATGGG 6825 0.0 22.827602 3 GTATCAA 11385 0.0 22.122849 1 AGAGTAC 9320 0.0 20.846855 10-11 AGTACTT 5925 0.0 20.60525 12-13 GAGTACT 5480 0.0 20.414722 12-13 GTACTTT 5935 0.0 18.846785 14-15 CAACGCA 13215 0.0 18.79852 5 TCAACGC 13265 0.0 18.763468 4 TATCAAC 13375 0.0 18.644667 2 ATCAACG 13305 0.0 18.635656 3 AACGCAG 13465 0.0 18.448557 6 CATGGGG 3910 0.0 18.3438 4 TAACGCG 65 0.0075689713 18.268116 76-77 GTATAGG 675 0.0 17.600882 1 CATGGGA 3380 0.0 17.144793 4 TACTTTT 7085 0.0 17.128452 14-15 GGTATCA 8860 0.0 17.002932 1 ACCGTGC 425 5.638867E-11 16.766232 8 >>END_MODULE