FastQCFastQC Report
Thu 26 May 2016
SRR937249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069403
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48740.23552686451116578No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45060.21774395804007243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.16995239689900904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30460.14719220954062595No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24610.11892318702543682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23760.11481572221553751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG70550.026.4890541
TACATGG72150.024.9692442
ACATGGG74550.023.5272253
TACTGCG1452.3349106E-522.9317957
GTATCAA113750.022.11441
GAGTACT50300.021.29440712-13
CATGGGG41800.021.150894
GGACCGA4350.020.747316
ATCAACG127950.019.614883
TCAACGC128450.019.5385274
CAACGCA129100.019.4750845
GTACTTT55050.019.45630814-15
AACGCAG130650.019.2328556
AGAGTAC88100.019.22076210-11
TATCAAC130800.019.1516132
AGTACTT53600.018.74271812-13
CATGGGA37450.018.1499824
GGACCGT2956.344817E-817.7120236
ACGCAGA142900.017.4848357
GAGTACA57150.017.473261
Produced by FastQC (version 0.11.5)