##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2069403 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.619530366970572 33.0 31.0 34.0 30.0 34.0 2 31.715635378899133 33.0 31.0 34.0 30.0 34.0 3 31.777710769724408 33.0 31.0 34.0 30.0 34.0 4 35.397733066009856 37.0 35.0 37.0 33.0 37.0 5 35.37878122337698 37.0 35.0 37.0 33.0 37.0 6 35.436388175720246 37.0 35.0 37.0 33.0 37.0 7 35.376504721409994 37.0 35.0 37.0 33.0 37.0 8 35.35447566278777 37.0 35.0 37.0 33.0 37.0 9 36.9653146342206 39.0 37.0 39.0 33.0 39.0 10-11 36.86656948888158 39.0 37.0 39.0 33.0 39.0 12-13 36.75153921203362 39.0 37.0 39.0 33.0 39.0 14-15 37.917673599584035 40.0 38.0 41.0 33.0 41.0 16-17 37.86329390650347 40.0 38.0 41.0 33.0 41.0 18-19 37.793221523308894 40.0 38.0 41.0 33.0 41.0 20-21 37.706715656641066 40.0 37.5 41.0 32.0 41.0 22-23 37.56949105611618 40.0 37.0 41.0 32.0 41.0 24-25 37.423089895974826 40.0 37.0 41.0 32.0 41.0 26-27 37.25470945002013 39.5 37.0 41.0 31.5 41.0 28-29 37.0758631837298 39.0 36.0 41.0 31.0 41.0 30-31 37.10631568621481 39.0 37.0 41.0 31.0 41.0 32-33 36.992339336513965 39.0 36.0 41.0 31.0 41.0 34-35 36.80495727511751 39.0 36.0 41.0 30.0 41.0 36-37 36.86207616399513 39.0 36.0 41.0 30.0 41.0 38-39 36.85469190872924 39.0 36.0 41.0 30.0 41.0 40-41 36.726718043802975 39.0 36.0 41.0 30.0 41.0 42-43 36.5267337971386 39.0 36.0 41.0 30.0 41.0 44-45 36.34740864877455 39.0 36.0 41.0 30.0 41.0 46-47 36.09724350452764 39.0 35.0 41.0 29.0 41.0 48-49 35.84306633362375 39.0 35.0 40.5 28.0 41.0 50-51 34.92783957498854 38.0 34.0 39.5 27.0 40.5 52-53 35.06840330278829 38.0 34.5 39.5 27.5 40.5 54-55 35.43984569462787 39.0 35.0 40.0 27.5 41.0 56-57 35.22022897425006 38.5 34.5 40.0 26.5 41.0 58-59 34.9723975948619 38.0 34.0 40.0 26.0 41.0 60-61 34.679860568482795 38.0 34.0 40.0 26.0 41.0 62-63 34.36066778679648 37.5 34.0 40.0 26.0 41.0 64-65 34.001617857903945 37.0 33.5 40.0 25.0 41.0 66-67 33.6002615247006 36.5 33.0 39.0 23.5 41.0 68-69 33.104277658822376 36.0 32.5 39.0 22.0 40.5 70-71 32.666417802622306 35.5 32.0 39.0 22.0 40.0 72-73 32.25397252251011 35.0 31.5 38.0 20.5 40.0 74-75 31.79687692537413 35.0 31.0 37.0 19.5 39.0 76-77 31.3599400406784 35.0 31.0 37.0 18.0 39.0 78-79 30.887891338709764 34.5 31.0 36.0 16.5 38.5 80-81 30.439115532354016 34.0 30.0 36.0 11.5 37.5 82-83 29.9992524414046 34.0 30.0 35.0 7.5 37.0 84-85 29.540949974461235 34.0 29.0 35.0 6.0 36.5 86-87 29.124600186623873 34.0 29.0 35.0 2.0 36.0 88-89 28.748727289947873 34.0 29.0 35.0 2.0 36.0 90-91 28.323782269572433 34.0 28.5 35.0 2.0 35.0 92-93 27.879515493115647 33.5 27.0 35.0 2.0 35.0 94-95 27.342543477515015 33.0 26.5 35.0 2.0 35.0 96-97 26.714672299209 33.0 25.0 35.0 2.0 35.0 98-99 26.006032899343435 32.5 24.5 35.0 2.0 35.0 100-101 24.15459821020845 30.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.006027942225252048 1101 2 -0.03327094251928031 1101 3 0.06226662534297489 1101 4 -0.06345229091635929 1101 5 0.06575726548972227 1101 6 -0.06779067220941926 1101 7 -0.020782326533051787 1101 8 -0.027679951597562535 1101 9 0.002868890866018603 1101 10-11 -0.06467525185541234 1101 12-13 0.023282455031967686 1101 14-15 0.1593929082552492 1101 16-17 0.1174479167995841 1101 18-19 0.13469996785118354 1101 20-21 0.17340389538463796 1101 22-23 -0.04293043375754735 1101 24-25 0.03937108988201743 1101 26-27 0.19149630009608387 1101 28-29 0.1644582223489266 1101 30-31 0.06560241971936165 1101 32-33 0.1301087015492257 1101 34-35 0.05786949046130019 1101 36-37 0.1745158403337399 1101 38-39 0.1940784074973294 1101 40-41 0.23434879630005412 1101 42-43 0.15024997631910963 1101 44-45 0.054013240520340844 1101 46-47 0.21130051773535996 1101 48-49 0.337285141365264 1101 50-51 0.28744721624095604 1101 52-53 0.4594860369209428 1101 54-55 0.3177658783803281 1101 56-57 0.4767334730200119 1101 58-59 0.47730937280487495 1101 60-61 0.3153498656004885 1101 62-63 0.512619480128798 1101 64-65 0.304419457121881 1101 66-67 0.33445253673114195 1101 68-69 0.364913495953445 1101 70-71 0.47657461937026824 1101 72-73 0.4969389515533962 1101 74-75 0.38721579656207794 1101 76-77 0.3941037405254306 1101 78-79 0.511442088195242 1101 80-81 0.6708282345178667 1101 82-83 0.5861896302326386 1101 84-85 0.5221597429050249 1101 86-87 0.7503043303007608 1101 88-89 0.7652735024753134 1101 90-91 0.8355415978453955 1101 92-93 0.731641445535022 1101 94-95 0.8107590397779205 1101 96-97 0.7437684048790274 1101 98-99 0.7004993573701555 1101 100-101 0.9965364934824805 1102 1 -0.015890298140774917 1102 2 -0.21990843794974424 1102 3 -0.09034232175310564 1102 4 -0.2162844496216607 1102 5 -0.20716350760952906 1102 6 -0.18978888344200584 1102 7 -0.15290791903535705 1102 8 -0.09357241832873342 1102 9 -0.05182979037150659 1102 10-11 -0.122879369955875 1102 12-13 -0.040927623371878497 1102 14-15 0.15312599636327207 1102 16-17 0.18096230364866983 1102 18-19 0.06388462442044585 1102 20-21 0.00739318184230342 1102 22-23 0.0075532836319496255 1102 24-25 0.08197506852133785 1102 26-27 0.18128129443432073 1102 28-29 0.07594782471559114 1102 30-31 0.08669876073995653 1102 32-33 -0.01702218377381115 1102 34-35 -0.12223622144999524 1102 36-37 0.015493556374750028 1102 38-39 0.14263566419591456 1102 40-41 0.043202872171711704 1102 42-43 -0.021116816618921064 1102 44-45 0.05570108615629721 1102 46-47 0.04999162146143021 1102 48-49 0.1787198877879561 1102 50-51 0.10488433190422342 1102 52-53 0.07574731010642921 1102 54-55 0.13737146715629223 1102 56-57 0.08880860617065167 1102 58-59 0.13902572250183454 1102 60-61 0.06226917644848129 1102 62-63 0.11400981789469 1102 64-65 0.07417107117855437 1102 66-67 0.18741757705834772 1102 68-69 0.20172090584097901 1102 70-71 0.05642495998318964 1102 72-73 -0.029735681246592094 1102 74-75 -0.03948417044810526 1102 76-77 -0.021049022609847867 1102 78-79 0.13194107682528156 1102 80-81 0.20929288299494075 1102 82-83 0.1513615016344687 1102 84-85 0.15807178523283127 1102 86-87 0.015574648475691788 1102 88-89 0.10650617475178592 1102 90-91 0.1842403295053785 1102 92-93 -0.12335448223665324 1102 94-95 -0.1355873920184827 1102 96-97 0.2277109275821978 1102 98-99 0.3717765274650908 1102 100-101 0.5330988471535889 1103 1 0.1406887548560789 1103 2 0.039315837999236436 1103 3 0.09000890035989073 1103 4 0.08870760592438387 1103 5 -0.0667382786290105 1103 6 0.04554613925535733 1103 7 0.07310808498760935 1103 8 0.09362451476366829 1103 9 0.24063532294866974 1103 10-11 0.12558028448687963 1103 12-13 0.10052940008584699 1103 14-15 0.12390153865366216 1103 16-17 0.15426462155103593 1103 18-19 0.12578752183625852 1103 20-21 0.10279478951503052 1103 22-23 0.10316217414995776 1103 24-25 -0.006011538749994827 1103 26-27 -0.0080276971122899 1103 28-29 -0.16395355499901854 1103 30-31 -0.15744582074862734 1103 32-33 -0.015226843207415186 1103 34-35 0.14683773846270043 1103 36-37 0.07924473213878969 1103 38-39 0.03891835649486097 1103 40-41 0.038681803843346074 1103 42-43 -0.078707746157896 1103 44-45 -0.12378124443901584 1103 46-47 -0.09847295566490999 1103 48-49 -0.0498701496092977 1103 50-51 -0.12565546685646467 1103 52-53 -0.13698320401864095 1103 54-55 -0.17502983303803887 1103 56-57 -0.11295341161461891 1103 58-59 -0.048863870036278456 1103 60-61 -0.18534021694058112 1103 62-63 -0.18074138967745768 1103 64-65 -0.2153686806273214 1103 66-67 -0.07983521713259023 1103 68-69 -0.2058335201059549 1103 70-71 -0.191226692751016 1103 72-73 -0.04303487892490665 1103 74-75 -0.051086784165885746 1103 76-77 -0.15029559452874253 1103 78-79 0.0052894407524632925 1103 80-81 -0.011234707510489272 1103 82-83 -0.06641824689970832 1103 84-85 -0.06413284996602187 1103 86-87 0.08451349965766397 1103 88-89 0.13859836071108234 1103 90-91 0.2751884232150985 1103 92-93 0.16584621027132584 1103 94-95 0.13773810036347456 1103 96-97 0.2457628626512438 1103 98-99 0.27448619970524035 1103 100-101 0.4797611162229245 1104 1 -0.043088620822462786 1104 2 0.046146223488428006 1104 3 -0.00887276623948452 1104 4 -0.10532452368043721 1104 5 0.040249561743301854 1104 6 -0.05544813878280763 1104 7 0.0043469135820046745 1104 8 -0.012416394143727416 1104 9 -0.09778917394810804 1104 10-11 0.003988778975958951 1104 12-13 0.05400386780392097 1104 14-15 -0.006776838643467897 1104 16-17 -0.0826613012483648 1104 18-19 -0.05808344590567316 1104 20-21 0.044629707796310925 1104 22-23 -0.0314738628317599 1104 24-25 0.03667818704361281 1104 26-27 0.07979432381562646 1104 28-29 0.06842195838097354 1104 30-31 -0.11753963302864179 1104 32-33 -0.13467637506358443 1104 34-35 -0.12611635738291227 1104 36-37 -0.22787377009457543 1104 38-39 -0.1973208574786227 1104 40-41 -0.0601716016231677 1104 42-43 -0.14584812324215335 1104 44-45 -0.03946213587588687 1104 46-47 -0.06001946357985588 1104 48-49 -0.15940002382890128 1104 50-51 -0.23737649644257175 1104 52-53 -0.2093427149909317 1104 54-55 -0.19464966574681597 1104 56-57 -0.19758963211018 1104 58-59 -0.3296804332623111 1104 60-61 -0.2371516821518611 1104 62-63 -0.11832759530839354 1104 64-65 -0.01714656280408633 1104 66-67 -0.15418401823661299 1104 68-69 -0.19349588398455353 1104 70-71 -0.31464122484575796 1104 72-73 -0.22600369258127273 1104 74-75 -0.1447533541819226 1104 76-77 -0.22833669429054027 1104 78-79 -0.08875369968334468 1104 80-81 -0.04608655677955653 1104 82-83 -0.07431411380078501 1104 84-85 -0.03999784249700866 1104 86-87 -0.10317712535380608 1104 88-89 -0.2221634419821683 1104 90-91 -0.11819993980465782 1104 92-93 -0.10414303581707784 1104 94-95 -0.061604920054275425 1104 96-97 -0.03530181129969989 1104 98-99 -0.018590556797573043 1104 100-101 0.20192885522219228 1105 1 0.008469746368657383 1105 2 0.15303877627209062 1105 3 0.08044971976961435 1105 4 0.025023336020801423 1105 5 -0.027397096079972982 1105 6 0.04708682442942802 1105 7 0.01654566214040898 1105 8 0.15583497206414165 1105 9 0.0908883432507892 1105 10-11 0.09791081811037117 1105 12-13 0.11754340081506598 1105 14-15 0.28960244985375994 1105 16-17 0.27506477066542345 1105 18-19 0.20528878979906295 1105 20-21 0.15477308944750234 1105 22-23 0.3660594241994133 1105 24-25 0.2656896732949221 1105 26-27 0.33472365955068284 1105 28-29 0.18885476597623807 1105 30-31 0.16828990015894618 1105 32-33 0.09607192349233884 1105 34-35 0.24463364141408306 1105 36-37 0.25764996029851517 1105 38-39 0.19976266766650497 1105 40-41 0.3418714946762478 1105 42-43 0.23679826992094632 1105 44-45 0.15039368047240487 1105 46-47 0.050173160902652114 1105 48-49 0.08738138772407211 1105 50-51 0.01387366195253037 1105 52-53 -0.02666079320523096 1105 54-55 0.06910303899990566 1105 56-57 0.17541599312139766 1105 58-59 0.07027738151086282 1105 60-61 0.22367147513166685 1105 62-63 0.3187952439017323 1105 64-65 0.23452103685650627 1105 66-67 0.16804382690030195 1105 68-69 0.24241569385520734 1105 70-71 0.3210235774370602 1105 72-73 0.34016375550123 1105 74-75 0.1862014595053374 1105 76-77 0.15659187152402865 1105 78-79 0.2918702085934619 1105 80-81 0.23937630177482205 1105 82-83 0.46034361644153066 1105 84-85 0.26364003181667783 1105 86-87 0.3553900044948861 1105 88-89 0.4718613334175892 1105 90-91 0.5945768907214024 1105 92-93 0.5380891882426191 1105 94-95 0.3422679404088633 1105 96-97 0.23652014137906363 1105 98-99 0.25010190969083723 1105 100-101 0.7976881973251295 1106 1 -0.07366749841857612 1106 2 -0.0060697313866704405 1106 3 -0.1437327906734751 1106 4 -0.049900957877625274 1106 5 -0.021594424636049325 1106 6 -0.05284877822550271 1106 7 -0.05200202457920966 1106 8 -0.05512908682133144 1106 9 -0.04801661799687196 1106 10-11 -0.15223019115738623 1106 12-13 -0.1774068051705271 1106 14-15 -0.3450186311625103 1106 16-17 -0.3212410106398522 1106 18-19 -0.1893693495733757 1106 20-21 -0.19112189730883244 1106 22-23 -0.08684544142342077 1106 24-25 -0.206364624424765 1106 26-27 -0.08409795868288228 1106 28-29 -0.0794166733156274 1106 30-31 -0.007999979783342326 1106 32-33 0.006747034777596639 1106 34-35 -0.13500617048889296 1106 36-37 -0.3867752803230573 1106 38-39 -0.3734821218075908 1106 40-41 -0.21050821881533466 1106 42-43 -0.1776331041368593 1106 44-45 -0.16312931375013306 1106 46-47 -0.18708660506305108 1106 48-49 -0.036959744137703865 1106 50-51 -0.1914094043472332 1106 52-53 -0.11219421352229375 1106 54-55 0.07476105921484333 1106 56-57 0.05389850180887379 1106 58-59 -0.042019597827824384 1106 60-61 0.0027770953688630584 1106 62-63 0.1402905846193434 1106 64-65 0.013588276425466006 1106 66-67 -0.033059014987408375 1106 68-69 -0.06277664091835078 1106 70-71 -0.12236652442206264 1106 72-73 0.21612154461038102 1106 74-75 0.13330804271443952 1106 76-77 0.16720800171424344 1106 78-79 0.10087745105357371 1106 80-81 0.08814442239956577 1106 82-83 -0.027881736165682014 1106 84-85 0.13879397471595922 1106 86-87 0.16453881652449098 1106 88-89 0.3130708903720709 1106 90-91 0.18506225769475648 1106 92-93 0.28964449245097157 1106 94-95 0.2231784232432723 1106 96-97 0.43760505361153434 1106 98-99 0.5933093158337606 1106 100-101 0.9395961890580651 1107 1 -0.025386990840637225 1107 2 -0.06370463409852789 1107 3 -0.06685755543327332 1107 4 0.03142276202730443 1107 5 0.04066957481144584 1107 6 0.04345264217145228 1107 7 -0.18678523300946637 1107 8 -0.01970385394873375 1107 9 -0.013209022257974823 1107 10-11 -0.1626351309068852 1107 12-13 0.06222554726145546 1107 14-15 0.10726964891085089 1107 16-17 0.0887598072614395 1107 18-19 0.08368671709251174 1107 20-21 0.1584522044549317 1107 22-23 -0.018061252634581137 1107 24-25 0.07823086910754284 1107 26-27 0.14642321663192348 1107 28-29 0.05362947746681357 1107 30-31 0.04178138602947001 1107 32-33 -0.029984219888604002 1107 34-35 -0.009617784350389513 1107 36-37 -0.1925754732130045 1107 38-39 -0.03263747869610967 1107 40-41 -0.010600415194744528 1107 42-43 -0.09811531777828719 1107 44-45 -0.08027011049829724 1107 46-47 -0.1064931942780234 1107 48-49 -0.11509748582800938 1107 50-51 -0.24977049859808886 1107 52-53 -0.21678432425340333 1107 54-55 -0.10561509302934269 1107 56-57 -0.19248172347146664 1107 58-59 -0.11541051740154984 1107 60-61 -0.1848641540324465 1107 62-63 0.04024871630819149 1107 64-65 -0.04963062746327296 1107 66-67 -0.17065574772440328 1107 68-69 -0.10415767380602858 1107 70-71 -0.17919625290690533 1107 72-73 -0.19588949499831187 1107 74-75 -0.2699960913554911 1107 76-77 -0.18877099712590706 1107 78-79 -0.07582853289572355 1107 80-81 -0.26045998906430157 1107 82-83 -0.051615566004407754 1107 84-85 0.03745591065650444 1107 86-87 0.11049837476795332 1107 88-89 0.18152971540539298 1107 90-91 0.3524992863136269 1107 92-93 0.34601099308554595 1107 94-95 0.2786718095661307 1107 96-97 0.44982565937131724 1107 98-99 0.5823779807998655 1107 100-101 0.9548031639406354 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-1.3707603264584556 2316 100-101 -1.67760461873155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8156.0 3 1644.0 4 1498.0 5 1688.0 6 1930.0 7 2283.0 8 2746.0 9 3426.0 10 4326.0 11 5191.0 12 6659.0 13 8065.0 14 9023.0 15 10511.0 16 12698.0 17 15863.0 18 14272.0 19 11472.0 20 11797.0 21 12827.0 22 13853.0 23 15798.0 24 17837.0 25 20332.0 26 23764.0 27 28132.0 28 33990.0 29 41205.0 30 50650.0 31 63592.0 32 80545.0 33 105106.0 34 142522.0 35 200662.0 36 295962.0 37 415593.0 38 331754.0 39 42025.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07621759392563 18.949403818393165 14.601723153407576 23.372655434273632 2 15.07935277288903 19.8840333072313 40.35696149045208 24.67965242942759 3 19.46882842684264 23.748138592875552 29.304617549586236 27.47841543069557 4 12.634381376830941 16.073963122140043 37.24905178901379 34.04260371201523 5 13.867120310172771 37.249367938670915 34.320566776588976 14.562944974567344 6 32.67640772195088 35.88404095930703 17.249163952478217 14.190387366263874 7 29.159606281099798 32.07549911013717 20.374396164509605 18.390498444253424 8 26.298580074160995 35.06341747834227 19.500360054180042 19.137642393316696 9 25.94285144251513 17.158610563771433 20.118215685030645 36.78032230868279 10-11 24.962539942059195 26.487312009592873 27.46301697059276 21.087131077755167 12-13 25.82370962570937 23.60121673755101 27.88401281245369 22.691060824285934 14-15 22.693229279271318 25.321390067879346 25.947089603785766 26.038291049063567 16-17 22.35807959654258 28.654765015062093 26.436601537614806 22.550553850780513 18-19 22.248162592941675 27.662173249987347 28.32043672146102 21.76922743560995 20-21 22.97420253011319 26.528805762363056 28.39866836147933 22.09832334604443 22-23 22.661564553793113 26.367335179035983 28.364374145797594 22.60672612137331 24-25 22.671239990306542 26.594136813810803 28.3195674451856 22.41505575069705 26-27 22.457077830103024 26.72479207776957 28.422962846385722 22.39516724574168 28-29 22.238070530000396 27.225009194628065 28.297179095374503 22.23974117999704 30-31 22.52585271525244 26.659672040325077 28.266539333150437 22.54793591127204 32-33 22.355132148653887 26.792752448616525 28.291088558933268 22.561026843796316 34-35 22.861411711839093 26.804443704106873 27.995292292375662 22.338852291678368 36-37 22.681071558964895 26.595818859197607 28.297221837536522 22.425887744300976 38-39 22.392837576673415 26.9160031946399 28.305293888229727 22.385865340456956 40-41 22.612922382377384 26.87264246981163 28.092160864871772 22.42227428293921 42-43 22.364624015044626 26.810807225217182 27.98723395189709 22.837334807841103 44-45 22.606276467725415 26.814647835487875 27.857279487073942 22.721796209712764 46-47 22.76273267744788 27.11449459083342 27.56842201086772 22.55435072085098 48-49 22.741224613526587 27.074143187228017 27.524204602161255 22.66042759708414 50-51 22.55587975350194 27.222368851168927 27.69354819182295 22.52820320350618 52-53 22.79857487718571 27.348279219654938 27.224272322149627 22.628873581009728 54-55 22.617950725331852 27.024862051705878 27.440299087544712 22.916888135417558 56-57 22.74205917696055 27.302942774172994 27.34755740351088 22.607440645355574 58-59 22.9700996935077 27.011867075968553 27.37318523976451 22.64484799075924 60-61 22.776816211126146 27.04066095536421 27.890118349354516 22.292404484155128 62-63 22.571069267647946 27.17805957630165 27.695972287615238 22.554898868435163 64-65 22.556938817353526 27.32649295325627 27.507148896759354 22.60941933263085 66-67 22.54137373385238 27.20203164642767 27.345883446632968 22.910711173086977 68-69 22.357098523660454 27.122982463444934 27.611504932912585 22.908414079982027 70-71 22.59809703089565 27.061921057575177 27.515246411840007 22.824735499689165 72-73 22.541146335204022 26.990349556215165 27.587534318876227 22.88096978970458 74-75 22.4022306607398 27.566467850974895 27.216317250388183 22.81498423789712 76-77 22.597214821070207 27.13318417775871 27.25363363946462 23.015967361706466 78-79 22.57279085352154 27.12685548161175 27.384284186958176 22.916069477908536 80-81 22.67106727075722 27.23636317862147 27.313028389609357 22.779541161011952 82-83 22.61271019674532 27.143972907329434 27.428012093697102 22.815304802228155 84-85 22.493420619459087 27.153597308989784 27.449524193142338 22.903457878408787 86-87 22.569816552338175 27.18710276830924 27.4503120723575 22.79276860699508 88-89 22.49271836844971 27.072998371422646 27.169328225760964 23.264955034366686 90-91 22.511649278287024 26.953674993850964 27.428119208042745 23.106556519819264 92-93 22.68390732942021 26.849120394524213 27.577934630902217 22.889037645153362 94-95 22.44588474198112 26.956167439914708 27.477323060803343 23.120624757300824 96-97 22.211498052703845 27.084168511320655 27.598807607914676 23.10552582806082 98-99 22.270060575072197 27.13461097565788 27.266844317391975 23.328484131877943 100-101 22.25109478379185 26.685976721910755 27.72391236307487 23.339016131222525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2709.0 1 1521.5 2 292.0 3 237.5 4 213.5 5 227.0 6 341.0 7 610.5 8 972.0 9 1220.0 10 1288.0 11 1296.0 12 1379.5 13 1503.5 14 1612.0 15 1715.5 16 1815.0 17 1933.0 18 2044.0 19 2138.0 20 2424.5 21 2896.5 22 3434.5 23 4194.0 24 5206.0 25 6751.5 26 8719.0 27 11082.0 28 13745.5 29 16779.5 30 20572.5 31 24753.0 32 29268.5 33 33471.5 34 37514.5 35 41472.5 36 46588.5 37 52281.5 38 56519.5 39 59523.0 40 61325.5 41 64780.0 42 68523.0 43 72130.0 44 76627.5 45 80102.0 46 82639.0 47 87539.0 48 94391.0 49 92635.0 50 84698.0 51 79177.5 52 75369.0 53 72964.5 54 70598.5 55 65348.5 56 57940.0 57 51175.5 58 45885.5 59 40487.5 60 33753.0 61 27217.5 62 21405.5 63 16185.0 64 12151.5 65 9184.0 66 6670.5 67 4915.0 68 3623.0 69 2488.0 70 1796.0 71 1305.0 72 957.5 73 740.5 74 580.0 75 410.5 76 253.5 77 160.5 78 107.0 79 74.5 80 40.5 81 21.0 82 18.5 83 14.0 84 10.0 85 9.0 86 9.5 87 9.0 88 6.5 89 6.0 90 6.0 91 5.5 92 4.0 93 5.0 94 3.5 95 2.5 96 3.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3112491863595443 2 0.2757800196481787 3 0.2791143146115087 4 0.27930760707315105 5 0.2667919201818109 6 0.2091907666123998 7 0.2163909108085762 8 0.21436133996133186 9 0.21088207565177008 10-11 0.2192419746178004 12-13 0.2192419746178004 14-15 0.20269130758967685 16-17 0.21511034825019582 18-19 0.202787953820498 20-21 0.20672628772646023 22-23 0.20515578647561641 24-25 0.19776234981779772 26-27 0.20914244349698924 28-29 0.20964983620880032 30-31 0.21670501105874496 32-33 0.21805805829024116 34-35 0.20677461084187082 36-37 0.20416516260969952 38-39 0.1966025950479438 40-41 0.19713414931746015 42-43 0.19720663399057603 44-45 0.212041830421624 46-47 0.2031987003014879 48-49 0.21027803670913783 50-51 0.2163909108085762 52-53 0.22221384621555107 54-55 0.22177893817685584 56-57 0.19024810537145254 58-59 0.1822747913287069 60-61 0.18884673502454571 62-63 0.18848431165896637 64-65 0.1872279106582913 66-67 0.1973032802213972 68-69 0.1879285958317447 70-71 0.1932441385269085 72-73 0.20358528522477254 74-75 0.19358240033478255 76-77 0.2039960317057625 78-79 0.2015557143775282 80-81 0.1898615204481679 82-83 0.20271546914738212 84-85 0.18647890236942732 86-87 0.19022394381374724 88-89 0.19829390408731407 90-91 0.19517706314333166 92-93 0.19959862820339971 94-95 0.19759321891386067 96-97 0.19208438375705456 98-99 0.19882545835683044 100-101 0.18423187750283537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2069403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.984875433765424 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02891478669443 44.665120287046086 2 12.954034825198502 15.022761914075888 3 4.269525184924543 7.427036580275217 4 1.9860691906627264 4.6064789849367 5 1.0163668791544667 2.9466953441388335 6 0.5992644271798645 2.084896389714674 7 0.4165087178530381 1.6905844285250036 8 0.29156852931132715 1.3525251882018792 9 0.20648736489281577 1.0775829718760752 >10 1.1323970505638974 11.747347362770096 >50 0.06544965824143913 2.590027553064807 >100 0.03141505513873589 2.9604199299457603 >500 9.99165092120971E-4 0.3964302508446145 >1k 9.991650921209565E-4 1.4320928145844378 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4874 0.23552686451116578 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4506 0.21774395804007243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3517 0.16995239689900904 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3046 0.14719220954062595 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2461 0.11892318702543682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.11481572221553751 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.664623082115953E-5 0.0 0.0 0.0 0.0 8 9.664623082115953E-5 0.0 0.0 0.0 0.0 9 9.664623082115953E-5 0.0 0.0 0.0 0.0 10-11 1.449693462317393E-4 0.0 0.0 0.0 0.0 12-13 1.6913090393702918E-4 0.0 0.0 4.8323115410579766E-5 9.664623082115953E-5 14-15 3.141002501687685E-4 0.0 0.0 4.8323115410579766E-5 1.449693462317393E-4 16-17 3.3826180787405836E-4 0.0 0.0 4.8323115410579766E-5 1.449693462317393E-4 18-19 3.865849232846381E-4 0.0 0.0 4.8323115410579766E-5 1.449693462317393E-4 20-21 4.349080386952179E-4 0.0 0.0 9.664623082115953E-5 1.9329246164231906E-4 22-23 4.8323115410579766E-4 0.0 0.0 1.449693462317393E-4 1.9329246164231906E-4 24-25 5.073927118110876E-4 0.0 0.0 3.3826180787405836E-4 1.9329246164231906E-4 26-27 5.315542695163775E-4 0.0 0.0 4.349080386952179E-4 1.9329246164231906E-4 28-29 7.006851734534066E-4 0.0 0.0 8.698160773904359E-4 1.9329246164231906E-4 30-31 8.214929619798561E-4 0.0 0.0 0.0021745401934760893 1.9329246164231906E-4 32-33 8.939776350957257E-4 0.0 0.0 0.0041074648098992795 1.9329246164231906E-4 34-35 0.0010389469813274648 0.0 0.0 0.005847096964680151 1.9329246164231906E-4 36-37 0.0011597547698539144 0.0 0.0 0.0081182833889774 1.9329246164231906E-4 38-39 0.0013047241160856536 0.0 0.0 0.011452578352307403 1.9329246164231906E-4 40-41 0.0013047241160856536 0.0 0.0 0.01889433812553669 1.9329246164231906E-4 42-43 0.0013772087892015234 0.0 0.0 0.02694013684139822 1.9329246164231906E-4 44-45 0.0015463396931385525 0.0 0.0 0.03578326696153432 1.9329246164231906E-4 46-47 0.0017154705970755817 0.0 0.0 0.05243058022047904 1.9329246164231906E-4 48-49 0.0017396321547808716 0.0 0.0 0.06526036736198798 1.9329246164231906E-4 50-51 0.0017396321547808716 0.0 0.0 0.08379228212194531 1.9329246164231906E-4 52-53 0.0017396321547808716 0.0 0.0 0.1048369988832528 1.9329246164231906E-4 54-55 0.0017396321547808716 0.0 0.0 0.12805625583803637 2.1745401934760895E-4 56-57 0.0017879552701914514 0.0 0.0 0.19317165385379262 2.4161557705289883E-4 58-59 0.0017879552701914514 0.0 0.0 0.2528990245012692 2.4161557705289883E-4 60-61 0.0018121168278967413 0.0 0.0 0.2992408921800152 2.4161557705289883E-4 62-63 0.0018362783856020312 0.0 0.0 0.3670865462164692 2.4161557705289883E-4 64-65 0.0018362783856020312 0.0 0.0 0.4351979773876814 2.4161557705289883E-4 66-67 0.0018846015010126108 0.0 0.0 0.5454230036392138 2.4161557705289883E-4 68-69 0.0018846015010126108 0.0 0.0 0.6810659885967112 2.4161557705289883E-4 70-71 0.0018846015010126108 0.0 0.0 0.7577547727533014 2.4161557705289883E-4 72-73 0.0018846015010126108 0.0 0.0 0.8451954500887454 2.4161557705289883E-4 74-75 0.0018846015010126108 0.0 0.0 1.0103396970044018 2.899386924634786E-4 76-77 0.0018846015010126108 0.0 0.0 1.2100349714386227 2.899386924634786E-4 78-79 0.0018846015010126108 0.0 0.0 1.408353037083642 2.899386924634786E-4 80-81 0.0021262170780655095 0.0 0.0 1.5635910453401296 3.141002501687685E-4 82-83 0.0021745401934760893 0.0 0.0 1.7797645021293582 3.3826180787405836E-4 84-85 0.002247024866591959 0.0 0.0 2.135760893359099 4.1074648098992804E-4 86-87 0.002271186424297249 0.0 0.0 2.4818510459296714 4.8323115410579766E-4 88-89 0.002271186424297249 0.0 0.0 2.908954901486081 4.8323115410579766E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7055 0.0 26.489054 1 TACATGG 7215 0.0 24.969244 2 ACATGGG 7455 0.0 23.527225 3 TACTGCG 145 2.3349106E-5 22.931795 7 GTATCAA 11375 0.0 22.1144 1 GAGTACT 5030 0.0 21.294407 12-13 CATGGGG 4180 0.0 21.15089 4 GGACCGA 435 0.0 20.74731 6 ATCAACG 12795 0.0 19.61488 3 TCAACGC 12845 0.0 19.538527 4 CAACGCA 12910 0.0 19.475084 5 GTACTTT 5505 0.0 19.456308 14-15 AACGCAG 13065 0.0 19.232855 6 AGAGTAC 8810 0.0 19.220762 10-11 TATCAAC 13080 0.0 19.151613 2 AGTACTT 5360 0.0 18.742718 12-13 CATGGGA 3745 0.0 18.149982 4 GGACCGT 295 6.344817E-8 17.712023 6 ACGCAGA 14290 0.0 17.484835 7 GAGTACA 5715 0.0 17.47326 1 >>END_MODULE