FastQCFastQC Report
Thu 26 May 2016
SRR937249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069403
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.2991684075068993No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48500.23436710974131186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47640.230211321816002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33240.16062603562476716No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21640.10457122174849462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114150.023.6083621
GTACATG66200.022.8311521
TACATGG66700.022.1504882
GAGTACT61900.021.48639912-13
AGAGTAC99750.020.76203210-11
ACATGGG67800.020.7400933
AGTACTT66100.020.26487412-13
TCAACGC132700.020.0119614
ATCAACG133150.019.9443243
CAACGCA133300.019.9218835
GTACTTT66950.019.75783714-15
GGTATCA88400.019.7320631
TATCAAC136100.019.6167452
AACGCAG135500.019.594646
ACACCGT4400.018.3510556
TAACGCA1350.00939600117.5948264
ACGCAGA151400.017.4125887
GGACCGT2202.9206758E-517.2715826
CATGGGG38350.017.0947384
CGCAGAG153150.017.089568
Produced by FastQC (version 0.11.5)