FastQCFastQC Report
Thu 26 May 2016
SRR937248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310564
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.21393045161267982No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47870.20717885330161812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.15745073497206744No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32550.14087469552888385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.11681130667663825No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25670.11109841579804756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG77850.025.3255671
TACATGG79100.024.441682
GTATCAA118950.023.4046881
ACATGGG82050.022.9260773
GAGTACT52000.020.8744412-13
TCAACGC135500.020.6512184
ATCAACG136700.020.4325223
CAACGCA136650.020.4083375
AGAGTAC97700.020.2753710-11
AGTACTT57250.020.24633212-13
TATCAAC138500.020.0640812
AACGCAG139350.020.0120436
GTACTTT55300.019.5422314-15
CATGGGG46050.019.2921944
ACGCAGA154100.018.0965487
GGTATCA92500.017.9761221
CGCAGAG154300.017.8875838
TGCACCG4405.456968E-1217.2761295
GTATAGG7700.017.2757531
CATGGGA42100.016.8141084
Produced by FastQC (version 0.11.5)