##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937248_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310564 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67524898682746 33.0 31.0 34.0 30.0 34.0 2 31.773325473780428 33.0 31.0 34.0 30.0 34.0 3 31.829533395309543 33.0 31.0 34.0 30.0 34.0 4 35.43254936889868 37.0 35.0 37.0 33.0 37.0 5 35.44208643430781 37.0 35.0 37.0 33.0 37.0 6 35.464391810830605 37.0 35.0 37.0 33.0 37.0 7 35.34630895313872 37.0 35.0 37.0 33.0 37.0 8 35.35021146352146 37.0 35.0 37.0 33.0 37.0 9 36.96776847557566 39.0 37.0 39.0 33.0 39.0 10-11 36.853454827479354 39.0 37.0 39.0 33.0 39.0 12-13 36.701488251353354 39.0 37.0 39.0 32.5 39.0 14-15 37.88298614537403 40.0 38.0 41.0 33.0 41.0 16-17 37.812124658741325 40.0 38.0 41.0 33.0 41.0 18-19 37.748365117780764 40.0 38.0 41.0 32.0 41.0 20-21 37.61664143473195 40.0 37.0 41.0 32.0 41.0 22-23 37.46006516157959 40.0 37.0 41.0 32.0 41.0 24-25 37.40025855159173 40.0 37.0 41.0 32.0 41.0 26-27 37.433590240304966 40.0 37.0 41.0 32.0 41.0 28-29 37.353548527545655 40.0 37.0 41.0 32.0 41.0 30-31 37.173797609587965 39.5 37.0 41.0 31.0 41.0 32-33 36.973602981782804 39.0 36.0 41.0 31.0 41.0 34-35 37.02974641689215 39.5 36.5 41.0 31.0 41.0 36-37 36.92276604326909 39.5 36.0 41.0 30.5 41.0 38-39 36.82413817578738 39.0 36.0 41.0 30.0 41.0 40-41 36.63971805152335 39.0 36.0 41.0 30.0 41.0 42-43 36.40685715695389 39.0 36.0 41.0 30.0 41.0 44-45 36.21839862475136 39.0 35.0 41.0 29.5 41.0 46-47 35.94624321161413 39.0 35.0 41.0 28.5 41.0 48-49 35.67818766327183 39.0 35.0 40.0 27.5 41.0 50-51 34.717874726690106 38.0 34.0 39.5 26.0 40.5 52-53 34.85052956767265 38.0 34.0 39.5 26.5 40.5 54-55 35.23811610498562 38.0 35.0 40.0 27.0 41.0 56-57 35.015974887516634 38.0 34.0 40.0 26.0 41.0 58-59 34.73528238127142 38.0 34.0 40.0 26.0 41.0 60-61 34.40627980873934 38.0 34.0 40.0 25.0 41.0 62-63 34.09087543127998 37.5 33.5 40.0 24.5 41.0 64-65 33.65651157033521 37.0 33.0 39.5 23.0 41.0 66-67 33.28147023843529 36.0 32.5 39.0 22.0 41.0 68-69 32.79017893466703 36.0 32.0 39.0 21.5 40.0 70-71 32.311491047207525 35.0 31.5 38.5 20.0 40.0 72-73 31.837689412628258 35.0 31.0 37.5 18.5 40.0 74-75 31.34723448474052 35.0 31.0 37.0 17.0 39.0 76-77 30.85131855252657 34.5 30.5 36.5 12.0 39.0 78-79 30.34101003045144 34.0 30.0 36.0 7.5 38.5 80-81 29.842270761597604 34.0 29.0 35.5 7.0 37.0 82-83 29.363995760342497 34.0 29.0 35.0 2.0 37.0 84-85 28.835014740989646 34.0 29.0 35.0 2.0 36.0 86-87 28.372309098557757 34.0 27.5 35.0 2.0 36.0 88-89 27.90060500379994 33.0 27.0 35.0 2.0 35.5 90-91 27.384441201369015 33.0 26.0 35.0 2.0 35.0 92-93 26.853913589928695 33.0 25.0 35.0 2.0 35.0 94-95 26.28387744291004 32.0 24.5 34.5 2.0 35.0 96-97 25.517246654929274 32.0 21.5 34.0 2.0 35.0 98-99 24.568550795390216 32.0 10.0 34.0 2.0 35.0 100-101 21.99541237550659 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.006237174751777985 1101 2 0.02991998127883022 1101 3 -0.07089444664502409 1101 4 0.011978452874728873 1101 5 -0.00864759182914554 1101 6 -7.352711336707785E-4 1101 7 0.06456700921020797 1101 8 -0.07193263540587935 1101 9 0.01901873010556443 1101 10-11 0.004696371193915638 1101 12-13 -0.011948059194111238 1101 14-15 -0.05959596743705475 1101 16-17 0.004039473391287629 1101 18-19 0.14602135210660805 1101 20-21 0.20743168836872883 1101 22-23 0.08431763681081605 1101 24-25 0.08100201420523234 1101 26-27 0.09217970079686921 1101 28-29 0.015855352711263038 1101 30-31 0.0985081074277474 1101 32-33 -0.15090847394921525 1101 34-35 -0.0028466617858171617 1101 36-37 0.06971422325104726 1101 38-39 0.14131367091139424 1101 40-41 -0.020916950937504453 1101 42-43 -0.09250206771805125 1101 44-45 0.02018754889385832 1101 46-47 0.05432922299861076 1101 48-49 -0.058776100576778845 1101 50-51 0.005358352358676655 1101 52-53 1.0219012501977431E-5 1101 54-55 0.02038516917069444 1101 56-57 -0.022946777649273997 1101 58-59 -0.053542375359491245 1101 60-61 0.019179783151471952 1101 62-63 0.0320673816468684 1101 64-65 -0.02155263865945045 1101 66-67 -0.08410646891076823 1101 68-69 -0.11455459785396016 1101 70-71 -0.13053234033400685 1101 72-73 -0.17129270287244935 1101 74-75 -0.269116320489168 1101 76-77 -0.18835973857964206 1101 78-79 -0.21579811494812517 1101 80-81 -0.13177288794172526 1101 82-83 -0.19069869395711692 1101 84-85 -0.20752722788815348 1101 86-87 -0.14294527447743377 1101 88-89 -0.194335116180806 1101 90-91 -0.2665947819767993 1101 92-93 -0.13682881226864652 1101 94-95 -0.21538819725589065 1101 96-97 -0.042187943064437405 1101 98-99 0.17370146884784177 1101 100-101 0.5703888827999606 1102 1 -0.02793629906914674 1102 2 -0.16622787386395643 1102 3 -0.041576579067022834 1102 4 -0.1976671543637778 1102 5 -0.06547076393948004 1102 6 -0.19285146430561184 1102 7 0.0898407143825608 1102 8 -0.042082955851810766 1102 9 -0.08282095344864615 1102 10-11 -0.12966278586371516 1102 12-13 -0.07243956542041019 1102 14-15 0.030093021745180693 1102 16-17 -0.10292416556137596 1102 18-19 -0.1310177677323381 1102 20-21 -0.15283342411344591 1102 22-23 -0.09991091787804152 1102 24-25 -0.1976608034145073 1102 26-27 -0.1380933902236876 1102 28-29 -0.15404403509353415 1102 30-31 -0.151929951287336 1102 32-33 -0.31416252191610283 1102 34-35 -0.1340047564151945 1102 36-37 -0.09176829570613876 1102 38-39 -0.09604934062813442 1102 40-41 -0.08985318024112843 1102 42-43 -0.04513900726377784 1102 44-45 0.15786584313887175 1102 46-47 -0.02570875114830784 1102 48-49 -0.18461091222240356 1102 50-51 -0.11117803803751514 1102 52-53 -0.16706420640229425 1102 54-55 -0.21232143040668205 1102 56-57 -0.2197359594469006 1102 58-59 -0.29282420364047823 1102 60-61 -0.09021437512360109 1102 62-63 -0.16025370995631505 1102 64-65 -0.29627211259766995 1102 66-67 -0.46890436405494995 1102 68-69 -0.49913767574362566 1102 70-71 -0.41985327323602206 1102 72-73 -0.31638688006121995 1102 74-75 -0.4956265088870211 1102 76-77 -0.41934834361807916 1102 78-79 -0.5874963696700313 1102 80-81 -0.5305148822284842 1102 82-83 -0.5766076331810872 1102 84-85 -0.4518221381459 1102 86-87 -0.4974603228269423 1102 88-89 -0.6118005878059058 1102 90-91 -0.5875166781731878 1102 92-93 -0.2953095963828787 1102 94-95 -0.3759300584945393 1102 96-97 -0.22612015592401136 1102 98-99 -0.4239735318043536 1102 100-101 -0.14708566522779876 1103 1 0.005111822394251675 1103 2 -0.2054909873539046 1103 3 -0.15769367105177068 1103 4 -0.04828899136711584 1103 5 -0.15469811109345244 1103 6 -0.1983691006088577 1103 7 -0.1431800046896896 1103 8 -0.11543299545556351 1103 9 -0.11647927805996261 1103 10-11 -0.12551547179799627 1103 12-13 -0.0744666397395406 1103 14-15 -0.2354670234066063 1103 16-17 -0.27633786032521357 1103 18-19 -0.3599386675144487 1103 20-21 -0.3756322270559309 1103 22-23 -0.3728874445141699 1103 24-25 -0.42101191046840825 1103 26-27 -0.6068755404033865 1103 28-29 -0.6860334216664157 1103 30-31 -0.5909886818540002 1103 32-33 -0.5854430188992765 1103 34-35 -0.5432585222847308 1103 36-37 -0.4886068056322088 1103 38-39 -0.5544391880151878 1103 40-41 -0.4167083748183771 1103 42-43 -0.4133847840121163 1103 44-45 -0.5313703679049411 1103 46-47 -0.41934565379172284 1103 48-49 -0.4464604195874031 1103 50-51 -0.5306841896581318 1103 52-53 -0.48426093638362744 1103 54-55 -0.2778187527282583 1103 56-57 -0.453521189017593 1103 58-59 -0.5424421510716684 1103 60-61 -0.380212137531899 1103 62-63 -0.26244706328204614 1103 64-65 -0.4410511868994931 1103 66-67 -0.21911312487504375 1103 68-69 -0.27311787575946056 1103 70-71 -0.4083156234585772 1103 72-73 -0.316716438404395 1103 74-75 -0.3800506411513922 1103 76-77 -0.4067408115276905 1103 78-79 -0.45623945657911946 1103 80-81 -0.6025929671526562 1103 82-83 -0.6065831196613587 1103 84-85 -0.5495967207796504 1103 86-87 -0.4178985340831609 1103 88-89 -0.3896758998999523 1103 90-91 -0.2963969445871264 1103 92-93 -0.3005824623135709 1103 94-95 -0.5335232637824987 1103 96-97 -0.7835612022737806 1103 98-99 -0.46685726054014154 1103 100-101 0.0415566246608563 1104 1 -0.10132806921767212 1104 2 -0.03582125102490252 1104 3 -0.16761018023575858 1104 4 -0.07956635085756858 1104 5 0.027643431336876745 1104 6 -0.09146845967936201 1104 7 0.04549204138523777 1104 8 -0.07430111996186639 1104 9 -0.011776395377061988 1104 10-11 -0.054252510723713954 1104 12-13 -0.07401248969603813 1104 14-15 -0.029217910808995384 1104 16-17 -0.2141902965572342 1104 18-19 0.007881672248181815 1104 20-21 -0.11203722250643011 1104 22-23 -0.2197136936267654 1104 24-25 -0.1622675723455984 1104 26-27 -0.11631350222758385 1104 28-29 -0.21062791627200994 1104 30-31 -0.18214665447701606 1104 32-33 -0.17979923971498124 1104 34-35 -0.09803689666355098 1104 36-37 -0.1986541004297777 1104 38-39 -0.19803619234472336 1104 40-41 -0.26415315589245836 1104 42-43 -0.18259457093555653 1104 44-45 -0.1296481573481003 1104 46-47 -0.08368537642223117 1104 48-49 -0.17066155746223188 1104 50-51 -0.3050032145653958 1104 52-53 -0.2153817130981892 1104 54-55 -0.2411867822450091 1104 56-57 -0.4859692197342156 1104 58-59 -0.4049936704045436 1104 60-61 -0.45574882849589926 1104 62-63 -0.25727343238144584 1104 64-65 -0.15501085133640657 1104 66-67 -0.326540107906915 1104 68-69 -0.33144106020917263 1104 70-71 -0.35104392334558554 1104 72-73 -0.1287219178016663 1104 74-75 -0.1319133362549323 1104 76-77 -0.24963809282674632 1104 78-79 -0.21413868766370214 1104 80-81 -0.09823584643593364 1104 82-83 -0.1325316653213342 1104 84-85 0.0063249278390031805 1104 86-87 0.11624311033595092 1104 88-89 -0.09042001804695587 1104 90-91 0.06287533546207058 1104 92-93 0.03350218837485386 1104 94-95 0.11829443627049585 1104 96-97 0.2711795762405842 1104 98-99 0.2606941088092327 1104 100-101 0.6980592093765381 1105 1 -0.09643491267473436 1105 2 -0.1167516169914471 1105 3 -0.20000662878271314 1105 4 -0.12440892135860793 1105 5 -0.0919776948544353 1105 6 -0.12718298752124468 1105 7 -0.1438761545965619 1105 8 -0.16028748259077474 1105 9 -0.1818757421913091 1105 10-11 -0.25894710401454546 1105 12-13 -0.12217639890191379 1105 14-15 -0.2447464567210389 1105 16-17 -0.2711620439920779 1105 18-19 -0.2195684913741971 1105 20-21 -0.2021113414879352 1105 22-23 -0.14513787323328842 1105 24-25 -0.17288646910052563 1105 26-27 -0.21165580545700635 1105 28-29 -0.23484670090324045 1105 30-31 -0.1519850529333624 1105 32-33 -0.21227032874902818 1105 34-35 -0.21729981370504703 1105 36-37 -0.3167732905316072 1105 38-39 -0.2802159553204291 1105 40-41 -0.25600234951443923 1105 42-43 -0.16851013918108038 1105 44-45 -0.11606280644002709 1105 46-47 -0.02426659439853296 1105 48-49 -0.16377403317962802 1105 50-51 -0.37032055071485814 1105 52-53 -0.33422410107226597 1105 54-55 -0.14912605291944914 1105 56-57 -0.34558402267029464 1105 58-59 -0.30325783905944803 1105 60-61 -0.10446429504783339 1105 62-63 0.03657743005842207 1105 64-65 0.002790155169371644 1105 66-67 -0.07465512596404267 1105 68-69 -0.23728342210797848 1105 70-71 -0.405664136902125 1105 72-73 -0.1716932610696773 1105 74-75 -0.17790830372602073 1105 76-77 -0.13830153919641575 1105 78-79 -0.12001625118960035 1105 80-81 -0.0941131492090399 1105 82-83 -0.08122705390818652 1105 84-85 -0.013705515049615258 1105 86-87 0.07040257370670133 1105 88-89 0.032209347932624866 1105 90-91 0.2988972908472647 1105 92-93 0.37097184326852783 1105 94-95 0.277644835874149 1105 96-97 0.19000390708372805 1105 98-99 0.20572755035497536 1105 100-101 0.5055202594280388 1106 1 -0.29716979593318626 1106 2 -0.21085277246418954 1106 3 -0.16807684555679359 1106 4 0.005108376147255456 1106 5 -0.11935843236401666 1106 6 -0.264065059266251 1106 7 -0.56263234887507 1106 8 -0.5211281860122341 1106 9 -0.6579165160802845 1106 10-11 -0.9800030035615919 1106 12-13 -0.4724315734549265 1106 14-15 -0.5939177338583264 1106 16-17 -0.557546303796741 1106 18-19 -0.726119565733633 1106 20-21 -0.572933817383614 1106 22-23 -0.5494076968159192 1106 24-25 -0.5598509510940275 1106 26-27 -0.7227203945568021 1106 28-29 -0.48670741623500646 1106 30-31 -0.5905733410590415 1106 32-33 -0.45180544308041704 1106 34-35 -0.21930589531118727 1106 36-37 -0.5910670211922096 1106 38-39 -0.5515242734056116 1106 40-41 -0.4555512006885607 1106 42-43 -0.5519628637989982 1106 44-45 -0.48451470425348475 1106 46-47 -0.4517466565223529 1106 48-49 -0.09906469547702557 1106 50-51 -0.20781740291355533 1106 52-53 -0.12340652655989715 1106 54-55 -0.4241517487051212 1106 56-57 -0.40324485234965834 1106 58-59 -0.4531574876945754 1106 60-61 -0.569989656328957 1106 62-63 -0.5604587461492798 1106 64-65 -0.5081335259153903 1106 66-67 -0.22726728766321713 1106 68-69 -0.4101491858104964 1106 70-71 -0.2445233250774166 1106 72-73 -0.35629883751450464 1106 74-75 -0.2512907121371626 1106 76-77 -0.16868484682495577 1106 78-79 -0.091286552481467 1106 80-81 -0.39437515975971493 1106 82-83 -0.42411029398734 1106 84-85 -0.6451389183821234 1106 86-87 -0.3753454087282968 1106 88-89 -0.21412447679995594 1106 90-91 -0.034691559289292684 1106 92-93 0.15110400228734377 1106 94-95 0.27494515719305923 1106 96-97 0.2577079791767538 1106 98-99 0.49232985957119624 1106 100-101 0.8072406611793497 1107 1 0.02158171213338278 1107 2 -0.03592251584625572 1107 3 0.030512464912131776 1107 4 0.044649984028360734 1107 5 0.05892516312354701 1107 6 0.01916493991122792 1107 7 0.11995649486176774 1107 8 0.010122095789562024 1107 9 0.1222922946082079 1107 10-11 -0.009532620690976046 1107 12-13 0.034900396845827686 1107 14-15 0.1276933143568968 1107 16-17 0.016816714844949843 1107 18-19 0.08808875089653156 1107 20-21 0.08007519726790235 1107 22-23 0.12750241380888383 1107 24-25 0.021788360645565774 1107 26-27 0.11993712820526525 1107 28-29 0.08313816594632328 1107 30-31 0.006803217458589472 1107 32-33 0.04827168521653391 1107 34-35 0.14947687396767861 1107 36-37 0.11493002156268517 1107 38-39 0.13386400642705354 1107 40-41 0.12215928168233603 1107 42-43 0.1484280653121104 1107 44-45 0.08803125642351972 1107 46-47 0.22318045757861427 1107 48-49 0.09847521927058978 1107 50-51 0.040020272148545644 1107 52-53 0.07101102431541051 1107 54-55 0.11638385095680093 1107 56-57 0.01912131536730044 1107 58-59 -0.06414913761708618 1107 60-61 0.035583337918602354 1107 62-63 0.22938987197670002 1107 64-65 0.30822645842120977 1107 66-67 0.12783316767382757 1107 68-69 0.07487473967413649 1107 70-71 0.20937082729967926 1107 72-73 0.2784910251083197 1107 74-75 0.2829998865177856 1107 76-77 0.19094793777026098 1107 78-79 0.2791748673842136 1107 80-81 0.35272033477271947 1107 82-83 0.36733028224642794 1107 84-85 0.22118059580114746 1107 86-87 0.34128597976959796 1107 88-89 0.3841433268566732 1107 90-91 0.5498465877242111 1107 92-93 0.5860477525998888 1107 94-95 0.9030687165881339 1107 96-97 0.8706200485460407 1107 98-99 1.0045500833348022 1107 100-101 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-0.3607208698026554 2316 12-13 0.039145039038245955 2316 14-15 0.0882778503467847 2316 16-17 0.11765596427427738 2316 18-19 0.19234960939608214 2316 20-21 0.3015864618891513 2316 22-23 0.18590127086071107 2316 24-25 0.26434893140880433 2316 26-27 0.4443679945337067 2316 28-29 0.2895022572666619 2316 30-31 0.2810508014351001 2316 32-33 0.5027170319773475 2316 34-35 0.2831060676363961 2316 36-37 0.043410236239935784 2316 38-39 0.21614256577159807 2316 40-41 0.1628168323090975 2316 42-43 0.14997162926291452 2316 44-45 0.0750710631733682 2316 46-47 -0.021889356638361335 2316 48-49 0.14694499965863628 2316 50-51 -0.06146493213726245 2316 52-53 0.0819340241481683 2316 54-55 -0.03934981952340166 2316 56-57 0.10127658634940673 2316 58-59 0.19690595688970802 2316 60-61 0.09689097522803536 2316 62-63 0.1832010056298614 2316 64-65 0.07188615842269286 2316 66-67 0.1881855978508824 2316 68-69 0.49476538908487555 2316 70-71 0.44677375556489096 2316 72-73 0.5201411343224471 2316 74-75 0.623664513928297 2316 76-77 0.46781266235167607 2316 78-79 0.600413088744105 2316 80-81 0.4981458605095206 2316 82-83 0.5166242381971031 2316 84-85 0.7080404681842047 2316 86-87 0.7302807559607594 2316 88-89 0.603260861444852 2316 90-91 0.974214810236063 2316 92-93 1.1185101972510303 2316 94-95 1.39307061748206 2316 96-97 1.4438193799645163 2316 98-99 1.5553393351283553 2316 100-101 1.2858018001893825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9331.0 3 2332.0 4 1816.0 5 1983.0 6 2366.0 7 2691.0 8 3329.0 9 4269.0 10 5190.0 11 6528.0 12 7947.0 13 9760.0 14 10712.0 15 12319.0 16 15054.0 17 18709.0 18 17200.0 19 14099.0 20 14612.0 21 15933.0 22 17574.0 23 19751.0 24 22434.0 25 25359.0 26 29656.0 27 35000.0 28 41366.0 29 49617.0 30 60745.0 31 75318.0 32 95189.0 33 123443.0 34 165076.0 35 230361.0 36 334235.0 37 447318.0 38 327645.0 39 34294.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14995869862491 18.85728813088718 14.581164143465012 23.4115890270229 2 15.096494366914978 19.85483013998677 40.327033386465416 24.721642106632835 3 19.446046691155743 23.683813356810465 29.289224083719677 27.580915868314115 4 12.676065500963709 16.006455373608432 37.15005924509775 34.16741988033011 5 13.900347540133232 37.227260366242156 34.26334546738358 14.609046626241037 6 32.78608374549857 35.79193641238591 17.213330597374608 14.208649244740906 7 29.278975595069213 31.97026844816501 20.383674631001995 18.367081325763788 8 26.383231054925826 35.039901375477214 19.471923286639665 19.104944282957295 9 25.996670463506526 17.05203289977644 20.029573710021488 36.921722926695544 10-11 24.97244255884018 26.44026401351735 27.406867561605686 21.18042586603678 12-13 25.871680901496823 23.563842432403355 27.822708689782505 22.741767976317316 14-15 22.71075680690734 25.267242654378315 25.91901327657603 26.102987262138317 16-17 22.377887517111073 28.61287906541825 26.413486789092033 22.59574662837864 18-19 22.324924802091076 27.563717879177148 28.237565750049935 21.873791568681842 20-21 23.069591346883684 26.431205417826536 28.33212033098916 22.16708290430062 22-23 22.69518772706996 26.319263860145398 28.28003796851528 22.705510444269365 24-25 22.709005760501576 26.545603031094217 28.22513490468413 22.520256303720082 26-27 22.49927457373345 26.682219054009447 28.333436707479937 22.48506966477716 28-29 22.335562888597863 27.171625724489783 28.172778314477043 22.320033072435315 30-31 22.529199750390365 26.61511509090645 28.21154958605535 22.644135572647833 32-33 22.4549615644977 26.706085803753254 28.185160193655673 22.65379243809337 34-35 22.9041288797726 26.735377209513793 27.942952635798544 22.417541274915056 36-37 22.748755245167747 26.57443947267807 28.15339517330713 22.52341010884706 38-39 22.41782902329947 26.86365609828985 28.206935407828542 22.511579470582138 40-41 22.700804575816232 26.78045632386356 28.014795590208035 22.50394351011217 42-43 22.484329752791453 26.732727161928093 27.84716187604462 22.935781209235834 44-45 22.6688999105054 26.70049501836526 27.789179699344995 22.841425371784343 46-47 22.79676129273243 27.064511028042492 27.48460937274612 22.654118306478953 48-49 22.84007418735561 27.011269102701764 27.463768587511794 22.68488812243083 50-51 22.58272063209856 27.10744712374244 27.66023400870385 22.64959823545515 52-53 22.875465600255495 27.22364259951609 27.148291728717773 22.752600071510646 54-55 22.698602796143074 26.936049577515625 27.40863164000466 22.95671598633664 56-57 22.742682638298998 27.19272545740762 27.31366856643706 22.75092333785632 58-59 23.041671698904427 26.97995698547962 27.262051405365778 22.716319910250174 60-61 22.778213828841526 26.984714121549835 27.774441143947442 22.462630905661197 62-63 22.600177198468792 27.09011578614582 27.661327149745716 22.648379865639672 64-65 22.60489412313645 27.21578420950269 27.409426892677807 22.769894774683046 66-67 22.608745238327028 27.102068976582615 27.28114192121701 23.00804386387334 68-69 22.403060770917037 27.054777738507475 27.501750901470324 23.040410589105164 70-71 22.653737471508414 26.97300975218826 27.433927276638116 22.939325499665213 72-73 22.62898364119436 26.89370473399892 27.47591107703403 23.00140054777269 74-75 22.439978489400826 27.491998577524896 27.142429656871247 22.92559327620303 76-77 22.70521531918142 27.032873660734037 27.168632788475133 23.09327823160941 78-79 22.64332979569142 27.0097442011659 27.324749021871646 23.022176981271027 80-81 22.6916371395487 27.114915061649153 27.284249649794646 22.909198149007498 82-83 22.679388549914773 27.033380307130138 27.356660351694167 22.930570791260923 84-85 22.581579660649428 27.049688296199925 27.29987531847997 23.068856724670677 86-87 22.61839344969018 27.093063650750764 27.353936270748914 22.93460662881014 88-89 22.59366429177357 26.97058445962942 27.080568551835643 23.355182696761368 90-91 22.63978391437249 26.82940317297151 27.30483926408574 23.225973648570257 92-93 22.804712164156822 26.755975478566295 27.439832129097702 22.999480228179177 94-95 22.55872827945413 26.82660751283961 27.444990518691025 23.169673689015234 96-97 22.343795370813524 26.936593030999674 27.537008488049047 23.182603110137755 98-99 22.51002422643377 26.94200207803857 27.14473120065824 23.40324249486942 100-101 22.658442119085084 26.349904933752843 27.61376889697785 23.377884050184225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2963.0 1 1710.5 2 414.5 3 342.5 4 283.0 5 247.5 6 320.5 7 581.0 8 949.0 9 1185.5 10 1198.5 11 1234.5 12 1388.5 13 1512.5 14 1643.5 15 1826.5 16 1927.0 17 2034.0 18 2129.0 19 2298.0 20 2664.5 21 3047.0 22 3678.0 23 4542.5 24 5632.0 25 7247.0 26 9465.5 27 12204.5 28 15037.0 29 18306.0 30 22547.5 31 27209.5 32 31919.0 33 36465.0 34 41182.5 35 45444.5 36 51102.0 37 57728.5 38 62464.5 39 65975.5 40 68159.5 41 71768.0 42 75903.5 43 79919.0 44 85232.0 45 89848.0 46 92648.5 47 97503.0 48 104750.0 49 103071.0 50 95241.0 51 88811.0 52 84030.5 53 82050.0 54 79800.0 55 73786.0 56 65464.5 57 58232.0 58 52606.5 59 46434.5 60 38702.5 61 31295.5 62 24817.5 63 18857.0 64 14148.5 65 10809.5 66 7783.0 67 5724.5 68 4244.5 69 2861.5 70 1994.5 71 1477.0 72 1166.0 73 903.0 74 647.5 75 463.0 76 295.0 77 169.5 78 104.5 79 73.5 80 46.5 81 32.0 82 23.5 83 14.5 84 16.5 85 11.0 86 10.5 87 11.5 88 8.0 89 7.5 90 6.0 91 3.0 92 2.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24046077061704416 2 0.21592130752491598 3 0.20661622010902966 4 0.2115500804132671 5 0.21341109789644433 6 0.23634922036351297 7 0.23968174004269088 8 0.22743364823480328 9 0.22236994950150696 10-11 0.2328003033025703 12-13 0.23457476183304163 14-15 0.21964334249127052 16-17 0.23390392994957077 18-19 0.22278110452686012 20-21 0.21596458700126894 22-23 0.21507735773603326 24-25 0.2104248140280901 26-27 0.21739280972091662 28-29 0.2302900936740986 30-31 0.23217275089545236 32-33 0.2310474845102754 34-35 0.2394220631845731 36-37 0.2159429472630925 38-39 0.21531539485597456 40-41 0.21107400617338448 42-43 0.21641902150297504 44-45 0.23357933387692353 46-47 0.23840499549027858 48-49 0.2418889933366918 50-51 0.2426680239110451 52-53 0.2601962118339938 54-55 0.24095848459510322 56-57 0.2135625760636797 58-59 0.19902067200908524 60-61 0.19536355625726012 62-63 0.20233155195008662 64-65 0.1952986370427307 66-67 0.1956448728535544 68-69 0.19947510651079128 70-71 0.1999511807506739 72-73 0.20256958907002792 74-75 0.20445224629138167 76-77 0.21683017652832812 78-79 0.2114202419842082 80-81 0.20661622010902966 82-83 0.2020502353537924 84-85 0.2029374646190281 86-87 0.20951594502467796 88-89 0.2067027790617356 90-91 0.2162459035975632 92-93 0.20533947555661736 94-95 0.20391125283696965 96-97 0.21252386863120865 98-99 0.21745772893544604 100-101 0.19651046238061357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310564.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.080056903491474 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46089995205023 43.64392520155196 2 13.218982559392526 15.090805533927734 3 4.410254877332931 7.552127980711934 4 1.9574156793379993 4.4691759344159765 5 1.0894973946810378 3.109428664229967 6 0.6438908355642506 2.2051995320186455 7 0.43473671435082994 1.7370357475227602 8 0.3007160837052707 1.3731912935753563 9 0.21991472300803128 1.129747041289259 >10 1.1557130175683255 11.863719242999402 >50 0.07198496800573144 2.7810801708969484 >100 0.03410146483740766 3.29930345427261 >500 9.080304794613617E-4 0.3547361111652402 >1k 9.836996860828511E-4 1.390524091422271 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4943 0.21393045161267982 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4787 0.20717885330161812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3638 0.15745073497206744 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3255 0.14087469552888385 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2699 0.11681130667663825 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2567 0.11109841579804756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.327947635295971E-5 0.0 0.0 0.0 0.0 8 4.327947635295971E-5 0.0 0.0 0.0 0.0 9 4.327947635295971E-5 0.0 0.0 0.0 0.0 10-11 4.327947635295971E-5 0.0 0.0 0.0 0.0 12-13 8.655895270591942E-5 0.0 0.0 0.0 0.0 14-15 1.7311790541183885E-4 0.0 0.0 0.0 4.327947635295971E-5 16-17 1.7311790541183885E-4 0.0 0.0 4.327947635295971E-5 4.327947635295971E-5 18-19 2.1639738176479855E-4 0.0 0.0 1.2983842905887912E-4 4.327947635295971E-5 20-21 2.5967685811775825E-4 0.0 0.0 1.7311790541183885E-4 4.327947635295971E-5 22-23 2.5967685811775825E-4 0.0 0.0 2.596768581177583E-4 8.655895270591942E-5 24-25 3.0295633447071794E-4 0.0 0.0 4.327947635295971E-4 8.655895270591942E-5 26-27 3.0295633447071794E-4 0.0 0.0 6.275524071179157E-4 8.655895270591942E-5 28-29 3.6787554900015755E-4 0.0 0.0 0.0010603471706475127 8.655895270591942E-5 30-31 4.327947635295971E-4 0.0 0.0 0.0018393777450007877 8.655895270591942E-5 32-33 4.327947635295971E-4 0.0 0.0 0.0035056375845897367 1.2983842905887912E-4 34-35 4.977139780590366E-4 0.0 0.0 0.0053666550677670035 1.7311790541183885E-4 36-37 6.491921452943957E-4 0.0 0.0 0.00750898914723851 1.7311790541183885E-4 38-39 6.708318834708755E-4 0.0 0.0 0.011620539400769682 1.7311790541183885E-4 40-41 6.924716216473554E-4 0.0 0.0 0.0210554652457149 1.7311790541183885E-4 42-43 7.141113598238352E-4 0.0 0.0 0.029971037374424598 1.947576435883187E-4 44-45 7.357510980003151E-4 0.0 0.0 0.04020663353189957 3.0295633447071794E-4 46-47 8.655895270591942E-4 0.0 0.0 0.05857877124373097 3.0295633447071794E-4 48-49 8.655895270591942E-4 0.0 0.0 0.07383478665814927 3.0295633447071794E-4 50-51 8.655895270591942E-4 0.0 0.0 0.09242332175174546 3.0295633447071794E-4 52-53 8.655895270591942E-4 0.0 0.0 0.11434437652451956 3.0295633447071794E-4 54-55 8.655895270591942E-4 0.0 0.0 0.13992254704911875 3.0295633447071794E-4 56-57 8.655895270591942E-4 0.0 0.0 0.20295910435720455 3.0295633447071794E-4 58-59 8.655895270591942E-4 0.0 0.0 0.2595037402123464 3.0295633447071794E-4 60-61 8.655895270591942E-4 0.0 0.0 0.3092967777564266 3.0295633447071794E-4 62-63 8.655895270591942E-4 0.0 0.0 0.3779380272522207 3.0295633447071794E-4 64-65 8.655895270591942E-4 0.0 0.0 0.4493058837582512 3.462358108236777E-4 66-67 9.088690034121539E-4 0.0 0.0 0.5587596794548864 3.462358108236777E-4 68-69 9.954279561180733E-4 0.0 0.0 0.6884033508701771 3.462358108236777E-4 70-71 9.954279561180733E-4 0.0 0.0 0.769076294792094 3.6787554900015755E-4 72-73 0.0010170676942945532 0.0 0.0 0.8628412803107812 3.895152871766374E-4 74-75 0.001038707432471033 0.0 0.0 1.0209628471663197 3.895152871766374E-4 76-77 0.001038707432471033 0.0 0.0 1.213859473271461 4.327947635295971E-4 78-79 0.0011036266470004726 0.0 0.0 1.4054793548241902 4.327947635295971E-4 80-81 0.0013849432432947108 0.0 0.0 1.5660678518318472 4.327947635295971E-4 82-83 0.0013849432432947108 0.0 0.0 1.76651674656058 4.327947635295971E-4 84-85 0.0014498624578241502 0.0 0.0 2.09392598517072 4.327947635295971E-4 86-87 0.0015147816723535899 0.0 0.0 2.4231746015258615 4.327947635295971E-4 88-89 0.0015147816723535899 0.0 0.0 2.8188572140827954 4.327947635295971E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7785 0.0 25.325567 1 TACATGG 7910 0.0 24.44168 2 GTATCAA 11895 0.0 23.404688 1 ACATGGG 8205 0.0 22.926077 3 GAGTACT 5200 0.0 20.87444 12-13 TCAACGC 13550 0.0 20.651218 4 ATCAACG 13670 0.0 20.432522 3 CAACGCA 13665 0.0 20.408337 5 AGAGTAC 9770 0.0 20.27537 10-11 AGTACTT 5725 0.0 20.246332 12-13 TATCAAC 13850 0.0 20.064081 2 AACGCAG 13935 0.0 20.012043 6 GTACTTT 5530 0.0 19.54223 14-15 CATGGGG 4605 0.0 19.292194 4 ACGCAGA 15410 0.0 18.096548 7 GGTATCA 9250 0.0 17.976122 1 CGCAGAG 15430 0.0 17.887583 8 TGCACCG 440 5.456968E-12 17.276129 5 GTATAGG 770 0.0 17.275753 1 CATGGGA 4210 0.0 16.814108 4 >>END_MODULE