FastQCFastQC Report
Thu 26 May 2016
SRR937248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310564
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66800.28910690203777084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51200.2215909189271537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.21843151715338766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.15082897509006457No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24250.10495273015592729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72600.023.3721
TACATGG75100.022.2635482
GTATCAA126600.021.5122181
GAGTACT67750.021.42104112-13
ACATGGG76000.021.1874053
GGTATCA101300.020.550811
GTACTTT72800.019.83421514-15
GGATCGT2502.1754931E-718.9990926
AGTACTT73750.018.84093512-13
AGAGTAC110950.018.75363310-11
ATCAACG147700.018.2345143
TATCAAC148000.018.2296472
CATGGGA41450.018.2206654
TCAACGC148350.018.154624
CAACGCA148650.018.0860255
AACGCAG151250.017.805766
CCTATAC5550.017.1170393
GCACCGT4204.7293724E-1116.9634746
AGGACCG8200.016.7987045
GGACCGA5700.016.6658696
Produced by FastQC (version 0.11.5)