##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937248_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310564 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23726717805696 33.0 31.0 34.0 31.0 34.0 2 32.44811872772189 34.0 31.0 34.0 31.0 34.0 3 32.51398619557822 34.0 31.0 34.0 31.0 34.0 4 36.1369726179409 37.0 35.0 37.0 35.0 37.0 5 36.05438628836942 37.0 35.0 37.0 35.0 37.0 6 36.09069257549239 37.0 35.0 37.0 35.0 37.0 7 36.03643179760439 37.0 35.0 37.0 35.0 37.0 8 36.00955567558397 37.0 35.0 37.0 35.0 37.0 9 37.73380308876967 39.0 37.0 39.0 35.0 39.0 10-11 37.649880288968404 39.0 37.0 39.0 35.0 39.0 12-13 37.556453099762656 39.0 37.0 39.0 35.0 39.0 14-15 38.79385033264605 40.0 38.0 41.0 35.0 41.0 16-17 38.76200399556126 40.0 38.0 41.0 35.0 41.0 18-19 38.729838255940976 40.0 38.0 41.0 34.5 41.0 20-21 38.61938556993012 40.0 38.0 41.0 34.0 41.0 22-23 38.47013023660024 40.0 38.0 41.0 34.0 41.0 24-25 38.4155768894521 40.0 38.0 41.0 34.0 41.0 26-27 38.38617064924408 40.0 38.0 41.0 34.0 41.0 28-29 38.27091805290829 40.0 38.0 41.0 34.0 41.0 30-31 38.38982322065089 40.0 38.0 41.0 34.0 41.0 32-33 38.36781668891231 40.0 38.0 41.0 34.0 41.0 34-35 38.265767362427525 40.0 38.0 41.0 34.0 41.0 36-37 38.123868674488136 40.0 38.0 41.0 33.5 41.0 38-39 38.01724708772404 40.0 38.0 41.0 33.0 41.0 40-41 37.872013499734265 40.0 37.0 41.0 33.0 41.0 42-43 37.69751346424509 40.0 37.0 41.0 33.0 41.0 44-45 37.46888400407866 40.0 37.0 41.0 32.5 41.0 46-47 37.195487551957015 40.0 37.0 41.0 31.5 41.0 48-49 36.93370038657228 40.0 36.0 41.0 31.0 41.0 50-51 36.72403794052015 39.0 36.0 41.0 31.0 41.0 52-53 36.501208579377156 39.0 36.0 41.0 30.5 41.0 54-55 36.284171526951866 39.0 35.0 40.0 30.0 41.0 56-57 36.01212799126101 39.0 35.0 40.0 29.5 41.0 58-59 35.74305645721131 38.0 35.0 40.0 29.0 41.0 60-61 35.405953697885025 38.0 34.5 40.0 28.0 41.0 62-63 35.04851802417072 37.5 34.0 40.0 28.0 41.0 64-65 34.65096184308247 37.0 34.0 40.0 27.5 41.0 66-67 34.22654144182979 36.5 33.5 39.0 26.0 40.5 68-69 33.79410395037748 36.0 33.0 39.0 26.0 40.0 70-71 33.37328678192857 35.5 33.0 38.5 26.0 40.0 72-73 32.90204707595202 35.0 32.0 37.5 25.5 39.5 74-75 32.425825253055095 35.0 32.0 37.0 24.5 39.0 76-77 31.152551714646293 34.0 30.5 35.5 23.0 38.0 78-79 31.770712258998238 34.5 31.0 36.0 24.0 38.5 80-81 31.581517759300326 34.5 31.0 36.0 24.0 37.0 82-83 31.24866244778331 34.0 31.0 35.0 23.0 37.0 84-85 30.898884428217528 34.0 31.0 35.0 21.0 36.5 86-87 30.498827775382978 34.0 31.0 35.0 20.0 36.0 88-89 30.04764832309341 34.0 30.0 35.0 18.0 36.0 90-91 29.554997610972904 34.0 29.5 35.0 13.0 35.0 92-93 29.211248638860468 34.0 29.0 35.0 7.0 35.0 94-95 28.779868032220705 34.0 29.0 35.0 2.0 35.0 96-97 28.30795078604185 33.5 29.0 35.0 2.0 35.0 98-99 27.56133524109265 33.0 28.0 35.0 2.0 35.0 100-101 25.023774498347592 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04147751374328834 1101 2 0.008213071246153447 1101 3 -0.01511506134944085 1101 4 -0.005963502126348885 1101 5 -0.025728292533159447 1101 6 0.0027734101355818552 1101 7 0.027363108793849733 1101 8 0.0063706916702201966 1101 9 -0.06516297092766621 1101 10-11 -0.039683808526262965 1101 12-13 -0.03175168736654399 1101 14-15 -0.11201684711098636 1101 16-17 -0.09859326541143076 1101 18-19 -0.012190862958199489 1101 20-21 -0.01833377128030378 1101 22-23 -0.08799869713357111 1101 24-25 0.012674220093984445 1101 26-27 -0.13747616000592444 1101 28-29 -0.24357968660674345 1101 30-31 -0.13225368586007136 1101 32-33 -0.006652170653495659 1101 34-35 0.01697569604597504 1101 36-37 0.003704218928824332 1101 38-39 0.02587325225160697 1101 40-41 0.1307693686582212 1101 42-43 0.1073623005645743 1101 44-45 0.11381023226130083 1101 46-47 0.09432601671829843 1101 48-49 0.1156034897379925 1101 50-51 0.21123548853704932 1101 52-53 0.09047905168159787 1101 54-55 0.16171541775915443 1101 56-57 0.15489503924929693 1101 58-59 0.19645136547959652 1101 60-61 0.19108431294888817 1101 62-63 0.1157258206317735 1101 64-65 -0.007506548843885241 1101 66-67 0.08262630335175203 1101 68-69 0.07922797024328787 1101 70-71 0.11865388656804043 1101 72-73 0.24253639285984718 1101 74-75 0.08010803969623481 1101 76-77 0.18555947231320502 1101 78-79 0.08652128887687027 1101 80-81 0.14563879210811592 1101 82-83 0.06945338159673398 1101 84-85 0.10875674646124622 1101 86-87 -0.015629916261833188 1101 88-89 0.09731804783101339 1101 90-91 0.15895535368593272 1101 92-93 0.22002090166770216 1101 94-95 0.2664040334687243 1101 96-97 0.18495678906182533 1101 98-99 0.41840304002445805 1101 100-101 1.0037734818825754 1102 1 -0.09060286375545701 1102 2 -0.0563614574331055 1102 3 -0.010752681353615401 1102 4 0.0062271392872688125 1102 5 0.020017320357723634 1102 6 -0.07790303730606496 1102 7 -0.04195560663972486 1102 8 -0.0942594389430127 1102 9 -0.125894159403785 1102 10-11 -0.09378161410210595 1102 12-13 -0.15133723273333288 1102 14-15 -0.05031605969229247 1102 16-17 -0.13631156104779762 1102 18-19 -0.20553522384901157 1102 20-21 -0.09829239484113117 1102 22-23 -0.07088927050133975 1102 24-25 -0.16253785249984531 1102 26-27 -0.16176559181224803 1102 28-29 -0.2780546629102574 1102 30-31 -0.1664484893749787 1102 32-33 -0.14192277245703622 1102 34-35 -0.06769722192508709 1102 36-37 -0.1242124586077793 1102 38-39 -0.10626082741202936 1102 40-41 -0.08913393232615618 1102 42-43 -0.03462959480569339 1102 44-45 -0.14136127913909746 1102 46-47 -0.14599946199400193 1102 48-49 -0.08197648043488215 1102 50-51 -0.1250414254425678 1102 52-53 -0.15195016868258193 1102 54-55 -0.09783083819758076 1102 56-57 -0.22321009592931773 1102 58-59 -0.2495686319550927 1102 60-61 -0.20629921017365405 1102 62-63 -0.2349708883841899 1102 64-65 -0.3068230739838924 1102 66-67 -0.28160212633276416 1102 68-69 -0.1534222415032147 1102 70-71 -0.07915299367127204 1102 72-73 -0.2236495233894189 1102 74-75 -0.08295687125718842 1102 76-77 -0.04497680720956154 1102 78-79 -0.39563719380948115 1102 80-81 -0.34086549391052756 1102 82-83 -0.37308538062108454 1102 84-85 -0.35049278721818666 1102 86-87 -0.2529461870451648 1102 88-89 -0.15997065677610323 1102 90-91 -0.30566488310491025 1102 92-93 -0.2865210378345395 1102 94-95 -0.217104224427203 1102 96-97 0.0042003660123306474 1102 98-99 0.0028198349564547698 1102 100-101 0.8527392883019296 1103 1 0.0347890188131359 1103 2 1.3259849101388E-4 1103 3 -0.07375980462411036 1103 4 -0.04591415219769601 1103 5 -0.0126038210464543 1103 6 -0.07324125280506166 1103 7 -0.048529748349004365 1103 8 -0.09664728438812631 1103 9 -0.039741068676200086 1103 10-11 -0.03388874285847976 1103 12-13 -0.06803639582539489 1103 14-15 -0.1329265671310722 1103 16-17 -0.20043633164736008 1103 18-19 -0.16759709974535753 1103 20-21 -0.19681250357511004 1103 22-23 -0.21509685361314723 1103 24-25 -0.1504109432166203 1103 26-27 -0.1944688863417383 1103 28-29 -0.22817744670134488 1103 30-31 -0.2634366720262378 1103 32-33 -0.1929863446532849 1103 34-35 -0.22162968124645488 1103 36-37 -0.15784619132961808 1103 38-39 -0.1792636079169867 1103 40-41 -0.18764667700532556 1103 42-43 -0.1293125580581176 1103 44-45 -0.2119011548879044 1103 46-47 0.028287042166212473 1103 48-49 0.023209043752054015 1103 50-51 0.11170982921671424 1103 52-53 0.20556178878587872 1103 54-55 0.09261024031081178 1103 56-57 0.051075002980070394 1103 58-59 -0.11757409729985824 1103 60-61 -0.07651125711876006 1103 62-63 0.09232135271253128 1103 64-65 -0.13368434897336812 1103 66-67 -0.04089468523236661 1103 68-69 -0.06043125429558671 1103 70-71 0.010559705499389338 1103 72-73 -0.03623723333591755 1103 74-75 0.09894210634176659 1103 76-77 0.24500730805127446 1103 78-79 0.14987867782301478 1103 80-81 0.20042435609786224 1103 82-83 0.21177623054763117 1103 84-85 -0.06959233491658878 1103 86-87 -0.11319986995085785 1103 88-89 0.0021693456614677586 1103 90-91 0.0733878129904113 1103 92-93 0.23845466078456212 1103 94-95 0.14396463907889157 1103 96-97 0.1851886329786403 1103 98-99 0.333123704233536 1103 100-101 0.9371772974182235 1104 1 0.039409310581035584 1104 2 0.04616593482401754 1104 3 0.04304814650127042 1104 4 -0.054079653992502585 1104 5 -0.018176008105875496 1104 6 -0.026960743661071263 1104 7 -0.02654776636702394 1104 8 -0.060531657107127046 1104 9 -0.008460509537705718 1104 10-11 -0.024421984201936198 1104 12-13 -0.04088678228288245 1104 14-15 -0.008056502838137192 1104 16-17 -0.09683992725184254 1104 18-19 0.0036052679941818155 1104 20-21 -0.06344457404735948 1104 22-23 -0.11360901727513806 1104 24-25 -8.57794063030326E-4 1104 26-27 -0.17621409436885926 1104 28-29 -0.08503299259129449 1104 30-31 -0.05124761282274193 1104 32-33 -0.032899154244226736 1104 34-35 -0.04259617493215018 1104 36-37 0.008716686753793113 1104 38-39 0.0011119507715591226 1104 40-41 -0.09771196982311636 1104 42-43 -0.04973551153323541 1104 44-45 -0.04388623652891255 1104 46-47 -0.09688818855840253 1104 48-49 -0.09189369494669108 1104 50-51 -0.17536039305883122 1104 52-53 -0.09287298338918504 1104 54-55 -0.05824476563853409 1104 56-57 -0.07888090187039154 1104 58-59 -0.13976585620637394 1104 60-61 -0.10621398981816554 1104 62-63 -0.19293046958701154 1104 64-65 -0.16907045650154373 1104 66-67 0.04594596229640757 1104 68-69 0.0045276002304106555 1104 70-71 0.04386624178039966 1104 72-73 0.018339763194468617 1104 74-75 0.039952795165994814 1104 76-77 0.0650864993402358 1104 78-79 -0.12548623203689502 1104 80-81 -0.04020077513770204 1104 82-83 -0.07820347168512143 1104 84-85 -0.01623420431095468 1104 86-87 0.01974190645373497 1104 88-89 0.07026065561737127 1104 90-91 0.1375223581904379 1104 92-93 0.24664915938971177 1104 94-95 0.30318552478146543 1104 96-97 0.2725008286371171 1104 98-99 0.4526883521222693 1104 100-101 1.0368347351000793 1105 1 -0.07203030559085022 1105 2 -0.05859723645145465 1105 3 -0.06905312988365608 1105 4 -0.07689891319381559 1105 5 -0.11967103424634473 1105 6 -0.13703701385311717 1105 7 -0.13897837352990194 1105 8 -0.1579992259784575 1105 9 -0.17084626722013496 1105 10-11 -0.14923593708390115 1105 12-13 -0.13393198377960402 1105 14-15 -0.205647864098097 1105 16-17 -0.19088505052273064 1105 18-19 -0.19491619847952535 1105 20-21 -0.1671284895113061 1105 22-23 -0.30765668288576364 1105 24-25 -0.18560747138145217 1105 26-27 -0.221783969598647 1105 28-29 -0.2037448737923242 1105 30-31 -0.1569318933406052 1105 32-33 -0.1579487624631568 1105 34-35 -0.23991589696966997 1105 36-37 -0.2687265017313578 1105 38-39 -0.3447817142393248 1105 40-41 -0.3422424844187475 1105 42-43 -0.33926439642391415 1105 44-45 -0.2398737725454012 1105 46-47 -0.3763024074082253 1105 48-49 -0.22059781875211826 1105 50-51 -0.41105966686230744 1105 52-53 -0.40707964301552835 1105 54-55 -0.1971518854935539 1105 56-57 -0.25675576660488275 1105 58-59 -0.16154725410210347 1105 60-61 -0.2065911035454988 1105 62-63 -0.10587169590740331 1105 64-65 -0.002066440908521372 1105 66-67 -0.09690021963081819 1105 68-69 -0.07196390574734579 1105 70-71 0.04054073103085898 1105 72-73 0.1033200763585711 1105 74-75 -0.002560926949747966 1105 76-77 0.07668947713713337 1105 78-79 0.005078493325225253 1105 80-81 -0.061971221416108335 1105 82-83 -0.09420802182136256 1105 84-85 -0.08640473663778181 1105 86-87 -0.024737500594412154 1105 88-89 0.20255906111404087 1105 90-91 0.2482469902030573 1105 92-93 0.29098834662325856 1105 94-95 0.4428275763844418 1105 96-97 0.2633124868945025 1105 98-99 0.3094603629174344 1105 100-101 0.661643212173388 1106 1 -0.12963993283713648 1106 2 -0.05580447471920991 1106 3 -0.08643277579518838 1106 4 -0.08313885509053165 1106 5 -0.11633706684946077 1106 6 -0.08199939695214908 1106 7 -0.0503968133228625 1106 8 -0.08873570880632542 1106 9 -0.11376028279864414 1106 10-11 -0.2143574611277046 1106 12-13 -0.10741074463208378 1106 14-15 -0.1599956937093694 1106 16-17 -0.09080590966443225 1106 18-19 -0.06395510043996921 1106 20-21 -0.10859316550663323 1106 22-23 -0.12887588982357556 1106 24-25 -0.10138622391185237 1106 26-27 -0.09128463914814233 1106 28-29 -0.247844624237878 1106 30-31 -0.14150496502184495 1106 32-33 -0.10064986238328544 1106 34-35 0.0018401060089843213 1106 36-37 -0.08537450675487435 1106 38-39 -0.2541280091285998 1106 40-41 -0.13729670766028335 1106 42-43 -0.3029607905837537 1106 44-45 -0.11605088811104025 1106 46-47 -0.21261863147331894 1106 48-49 -0.36964864182784396 1106 50-51 -0.09520766377988821 1106 52-53 0.014659126764286157 1106 54-55 0.007506130889055385 1106 56-57 -0.07316680838930267 1106 58-59 -0.09404880233397961 1106 60-61 0.04982487204593866 1106 62-63 0.1729855360377286 1106 64-65 0.17572934703612475 1106 66-67 0.2287246932304825 1106 68-69 0.2590260493890568 1106 70-71 0.07258183564162124 1106 72-73 0.006794573493941414 1106 74-75 0.1332799385902277 1106 76-77 0.18744209616184904 1106 78-79 0.11147647376076009 1106 80-81 -0.02907750848045154 1106 82-83 -0.09278178412751004 1106 84-85 0.05251516492385022 1106 86-87 -0.09398899397619331 1106 88-89 0.03413331842207157 1106 90-91 0.07700092563017336 1106 92-93 0.3679978867578555 1106 94-95 0.387191607039977 1106 96-97 0.4362944355375191 1106 98-99 0.5784752443169268 1106 100-101 1.3056802497708375 1107 1 -0.046071252308962585 1107 2 -0.10248014264752925 1107 3 -0.04714953746150741 1107 4 -0.007412393977269005 1107 5 -0.0793593429225794 1107 6 -0.07348085323277331 1107 7 -0.09829191446682017 1107 8 -0.0632863767004821 1107 9 0.013558655620869331 1107 10-11 -0.005109279620292284 1107 12-13 -0.05415662732001891 1107 14-15 -0.0522133050849547 1107 16-17 0.036479759064548034 1107 18-19 0.06457855741972907 1107 20-21 -0.06088190160543405 1107 22-23 0.06361529814894595 1107 24-25 0.04488878676588115 1107 26-27 -0.09435651780216858 1107 28-29 -0.06780333703013497 1107 30-31 -0.09795541832308885 1107 32-33 -0.11312264886948498 1107 34-35 -0.0689558826090888 1107 36-37 -0.07387070854490929 1107 38-39 -0.03722766216739615 1107 40-41 0.07568860968065394 1107 42-43 0.03868450013595748 1107 44-45 0.16276974986362092 1107 46-47 -0.0734420201067465 1107 48-49 0.017093953514063287 1107 50-51 0.08589572584822491 1107 52-53 0.08762720944910285 1107 54-55 0.11584132923789525 1107 56-57 -0.006420977621225177 1107 58-59 -0.08087482466989826 1107 60-61 -0.0750819796521256 1107 62-63 -0.09733192922448097 1107 64-65 -0.059711371840592165 1107 66-67 0.00884961237898807 1107 68-69 0.20528115290492366 1107 70-71 0.1120546497273125 1107 72-73 0.2143346603968297 1107 74-75 0.3141824803945852 1107 76-77 0.3698121250757396 1107 78-79 0.3056083971696957 1107 80-81 0.1885426114981108 1107 82-83 0.11467693891208697 1107 84-85 0.18276600336103854 1107 86-87 0.2632962621266408 1107 88-89 0.42093282064738347 1107 90-91 0.34158719141004923 1107 92-93 0.5613964423477533 1107 94-95 0.5077647912048597 1107 96-97 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0.24074888917530401 2316 84-85 0.241544563181602 2316 86-87 -0.17287289394944239 2316 88-89 -0.08397967317629806 2316 90-91 0.05162438736618924 2316 92-93 0.3822873300842602 2316 94-95 0.26272687011639206 2316 96-97 0.39776715307083066 2316 98-99 0.5193234520964864 2316 100-101 0.24876116282876382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 12.0 5 38.0 6 70.0 7 182.0 8 433.0 9 840.0 10 1503.0 11 2214.0 12 3540.0 13 4922.0 14 6410.0 15 8703.0 16 11662.0 17 16185.0 18 14801.0 19 9904.0 20 9049.0 21 9639.0 22 10381.0 23 11684.0 24 13697.0 25 16161.0 26 20124.0 27 25372.0 28 32138.0 29 40742.0 30 51331.0 31 65326.0 32 84422.0 33 111597.0 34 153982.0 35 222294.0 36 349512.0 37 539673.0 38 425834.0 39 36177.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.650156155732155 19.358070226119388 15.096064684983801 22.895708933164656 2 15.845871397632786 20.375631231162608 39.30425644994036 24.474240921264247 3 19.747819147186576 23.634705638969532 29.7150825512732 26.902392662570694 4 13.290391436895927 16.558121739973444 36.82927631522001 33.32221050791062 5 13.998302548816868 37.15669569184119 34.36581241563058 14.47918934371136 6 33.026784802325324 36.11365017372382 16.794038165573426 14.065526858377437 7 29.400873552950706 32.11505935347387 20.249471557593733 18.23459553598169 8 26.65487733730812 35.05135542664043 19.267849754432252 19.025917481619206 9 26.116394092524597 16.897259716675237 19.969193668731965 37.0171525220682 10-11 25.114939718050916 26.52410087775296 27.270478702339997 21.090480701856134 12-13 25.99314588242219 23.569534061969254 27.898716952125053 22.5386031034835 14-15 22.78649282166605 25.26902522501 25.990320977908425 25.954160975415526 16-17 22.397670006111063 28.594641827709598 26.53272101530189 22.474967150877447 18-19 22.3313009291238 27.4262690840851 28.56917185587588 21.673258130915222 20-21 22.93812246706865 26.260384910350894 28.805715833882985 21.995776788697476 22-23 22.723573286048683 26.158844307545554 28.700062387864683 22.417520018541076 24-25 22.77827597608879 26.385688589892958 28.642599071735365 22.19343636228289 26-27 22.57529035231795 26.50899892017286 28.772416151097897 22.143294576411297 28-29 22.346346470253064 26.948167246159215 28.67147500853171 22.034011275056013 30-31 22.58025773882996 26.41727791925407 28.66411807374502 22.33834626817095 32-33 22.558236863380543 26.490869761668577 28.570881395191822 22.380011979759054 34-35 22.991291303768257 26.489917613188812 28.383221585725387 22.13556949731754 36-37 22.822944018405902 26.301338678062447 28.635228729967544 22.240488573564104 38-39 22.550881272666405 26.620409292071308 28.640858531332686 22.187850903929604 40-41 22.77255983015384 26.55458424283448 28.47128284618263 22.201573080829053 42-43 22.543569397766575 26.562210198334984 28.26499145027601 22.629228953622427 44-45 22.798597396909233 26.458228109969827 28.159178658343148 22.583995834777788 46-47 22.918776541138875 26.87722564707145 27.856142482960873 22.347855328828807 48-49 22.950846633116416 26.91044264517235 27.700509485995628 22.43820123571561 50-51 22.758988714443745 26.92407568022353 27.931513691029547 22.385421914303176 52-53 22.99994719903885 27.12735938065338 27.363405644682427 22.509287775625346 54-55 22.800342254099 26.804667604965715 27.652252869862075 22.74273727107321 56-57 22.90841110655234 27.060990303666117 27.539033759722738 22.491564830058806 58-59 23.213444855888 26.789952583005704 27.513758545532607 22.482844015573686 60-61 22.904321196036985 26.892524941962222 28.040599611177186 22.162554250823607 62-63 22.70666382753302 26.94177698605189 27.91846492890913 22.43309425750596 64-65 22.719479468969368 27.03435763613508 27.708305787743985 22.537857107151567 66-67 22.721357209754846 26.953808680478016 27.584823445704167 22.740010664062975 68-69 22.602663245856856 26.842580426250905 27.76140370922424 22.793352618668 70-71 22.755418157644627 26.85298914031379 27.692221466274034 22.699371235767543 72-73 22.7528646685398 26.765694436509875 27.704880713107276 22.77656018184305 74-75 22.65617831836729 27.305043270820455 27.23973454100384 22.79904386980841 76-77 22.79744252918335 26.838209199139257 27.442152651906632 22.922195619770758 78-79 22.755071921833803 26.8669684111758 27.54753817682609 22.83042149016431 80-81 22.767822654516962 26.986923319787575 27.4670226548632 22.778231370832266 82-83 22.687653223235856 26.908732239413112 27.593025668902126 22.810588868448907 84-85 22.58594483049938 26.957605801557378 27.535173413106477 22.921275954836766 86-87 22.59831365848338 26.955193623721307 27.5074181022469 22.93907461554841 88-89 22.60729414982662 26.918146391963177 27.256548617566967 23.218010840643235 90-91 22.67644219355517 26.669308350020593 27.497564810873286 23.156684645550946 92-93 22.68210455153448 26.537384768231554 27.68317249919011 23.097338181043853 94-95 22.413899809743423 26.663165357029712 27.688997145285736 23.233937687941125 96-97 22.24976239567482 26.719904750528446 27.770687156901953 23.259645696894786 98-99 22.356496971947507 26.732512147482794 27.335222917724312 23.575767962845386 100-101 22.083363451542436 26.483211785619527 27.75334896450351 23.68007579833453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 137.0 1 104.0 2 114.0 3 164.5 4 138.5 5 124.5 6 254.5 7 632.0 8 1127.0 9 1447.0 10 1448.0 11 1398.0 12 1527.0 13 1706.5 14 1892.5 15 2014.5 16 2121.0 17 2229.0 18 2441.0 19 2598.5 20 2823.5 21 3352.5 22 3944.0 23 4810.5 24 5889.5 25 7427.0 26 9615.5 27 12325.5 28 14914.0 29 18191.0 30 22617.5 31 27133.0 32 31792.0 33 36116.0 34 40861.5 35 45504.0 36 51116.0 37 57374.5 38 62279.0 39 65942.5 40 68250.0 41 72076.0 42 76334.5 43 80506.5 44 85761.0 45 90072.0 46 92575.5 47 97967.0 48 106183.0 49 104184.5 50 95582.0 51 89298.0 52 84905.5 53 82802.5 54 80068.5 55 73972.5 56 65470.0 57 58113.0 58 52138.0 59 45873.0 60 38460.0 61 30773.0 62 24007.5 63 18215.0 64 13588.0 65 10073.5 66 7250.5 67 5430.0 68 3860.0 69 2511.5 70 1822.5 71 1372.0 72 1030.0 73 761.5 74 588.5 75 441.0 76 262.0 77 134.0 78 88.5 79 69.5 80 45.5 81 23.0 82 14.0 83 9.0 84 7.0 85 5.5 86 4.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07145441545873649 2 0.0 3 0.0 4 0.0 5 0.001774458530471348 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1639738176479855E-4 12-13 0.005539772973178843 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.006703125297545E-4 24-25 6.924716216473554E-4 26-27 3.8951528717663734E-4 28-29 0.002834805701118861 30-31 3.462358108236777E-4 32-33 0.0 34-35 0.0 36-37 4.327947635295971E-5 38-39 5.842729307649561E-4 40-41 1.0819869088239927E-4 42-43 0.011057906208181206 44-45 1.2983842905887912E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.1639738176479856E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1639738176479856E-5 82-83 2.380371199412784E-4 84-85 5.193537162355165E-4 86-87 0.0 88-89 0.0 90-91 0.007292591765473711 92-93 0.0024885698902951834 94-95 0.0 96-97 0.0 98-99 1.2983842905887912E-4 100-101 0.009651323226710015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310564.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.549172039653314 #Duplication Level Percentage of deduplicated Percentage of total 1 74.44977894420177 40.61173799941421 2 14.070812607312952 15.351023553080742 3 4.868040262201466 7.966426972763604 4 2.1900655223825205 4.778650438342294 5 1.2307391757160562 3.356790151603813 6 0.7370391453884239 2.412292508505131 7 0.4774700595841853 1.8231917496828869 8 0.3341154808456061 1.4580578276606768 9 0.24634965496465316 1.2094352739518492 >10 1.2752639271103745 12.507095006356653 >50 0.07918118209899934 2.9334263774974487 >100 0.03892267597674044 3.653789045716583 >500 0.0011106811081857259 0.39156341209812157 >1k 8.72678013574089E-4 0.8174448818654546 >5k 2.3800309461111517E-4 0.7290748014604962 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6680 0.28910690203777084 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5120 0.2215909189271537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5047 0.21843151715338766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3485 0.15082897509006457 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2425 0.10495273015592729 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.327947635295971E-5 2 8.655895270591942E-5 0.0 0.0 0.0 4.327947635295971E-5 3 8.655895270591942E-5 0.0 0.0 0.0 4.327947635295971E-5 4 8.655895270591942E-5 0.0 0.0 4.327947635295971E-5 4.327947635295971E-5 5 8.655895270591942E-5 0.0 0.0 4.327947635295971E-5 4.327947635295971E-5 6 8.655895270591942E-5 0.0 0.0 4.327947635295971E-5 4.327947635295971E-5 7 8.655895270591942E-5 0.0 0.0 4.327947635295971E-5 4.327947635295971E-5 8 8.655895270591942E-5 0.0 0.0 4.327947635295971E-5 4.327947635295971E-5 9 8.655895270591942E-5 0.0 0.0 8.655895270591942E-5 4.327947635295971E-5 10-11 8.655895270591942E-5 0.0 0.0 1.2983842905887912E-4 4.327947635295971E-5 12-13 1.0819869088239927E-4 0.0 0.0 2.1639738176479855E-4 4.327947635295971E-5 14-15 1.2983842905887912E-4 0.0 0.0 2.1639738176479855E-4 6.491921452943958E-5 16-17 1.51478167235359E-4 0.0 0.0 2.1639738176479855E-4 8.655895270591942E-5 18-19 1.7311790541183885E-4 0.0 0.0 2.1639738176479855E-4 8.655895270591942E-5 20-21 1.7311790541183885E-4 0.0 0.0 2.5967685811775825E-4 8.655895270591942E-5 22-23 1.7311790541183885E-4 0.0 0.0 3.6787554900015755E-4 8.655895270591942E-5 24-25 2.5967685811775825E-4 0.0 0.0 5.193537162355165E-4 8.655895270591942E-5 26-27 2.5967685811775825E-4 0.0 0.0 7.357510980003151E-4 8.655895270591942E-5 28-29 2.813165962942381E-4 0.0 0.0 0.0012334650760593517 8.655895270591942E-5 30-31 3.0295633447071794E-4 0.0 0.0 0.0020124956504126265 8.655895270591942E-5 32-33 3.2459607264719785E-4 0.0 0.0 0.0037220349663545354 8.655895270591942E-5 34-35 3.895152871766374E-4 0.0 0.0 0.005561412711355323 8.655895270591942E-5 36-37 4.327947635295971E-4 0.0 0.0 0.007725386529003308 8.655895270591942E-5 38-39 4.760742398825568E-4 0.0 0.0 0.012053334164299278 8.655895270591942E-5 40-41 5.193537162355165E-4 0.0 0.0 0.021877775296421133 8.655895270591942E-5 42-43 6.491921452943957E-4 0.0 0.0 0.031031384545072115 8.655895270591942E-5 44-45 7.357510980003151E-4 0.0 0.0 0.041331899917076524 8.655895270591942E-5 46-47 8.655895270591942E-4 0.0 0.0 0.06018011186879048 8.655895270591942E-5 48-49 9.088690034121539E-4 0.0 0.0 0.07560924518862061 1.2983842905887912E-4 50-51 9.088690034121539E-4 0.0 0.0 0.09424105975856976 1.2983842905887912E-4 52-53 9.088690034121539E-4 0.0 0.0 0.11631359269857922 1.2983842905887912E-4 54-55 9.088690034121539E-4 0.0 0.0 0.14251931563029632 1.2983842905887912E-4 56-57 9.088690034121539E-4 0.0 0.0 0.20629162403638246 1.2983842905887912E-4 58-59 9.088690034121539E-4 0.0 0.0 0.26389660706217183 1.2983842905887912E-4 60-61 9.088690034121539E-4 0.0 0.0 0.3144470354424288 1.2983842905887912E-4 62-63 9.088690034121539E-4 0.0 0.0 0.3836725578689878 1.7311790541183885E-4 64-65 9.088690034121539E-4 0.0 0.0 0.4555164886149009 1.7311790541183885E-4 66-67 9.088690034121539E-4 0.0 0.0 0.5655761969804776 2.380371199412784E-4 68-69 9.954279561180733E-4 0.0 0.0 0.6958257810647097 3.0295633447071794E-4 70-71 0.0010170676942945532 0.0 0.0 0.7772561158228035 3.0295633447071794E-4 72-73 0.001038707432471033 0.0 0.0 0.8719083306067263 3.0295633447071794E-4 74-75 0.001038707432471033 0.0 0.0 1.031631238087324 3.0295633447071794E-4 76-77 0.0010603471706475127 0.0 0.0 1.2268433161773489 3.0295633447071794E-4 78-79 0.0010819869088239927 0.0 0.0 1.4210166868349026 3.0295633447071794E-4 80-81 0.0012767445524123114 0.0 0.0 1.5838773563510902 3.0295633447071794E-4 82-83 0.0013849432432947108 0.0 0.0 1.7869230196610006 3.0295633447071794E-4 84-85 0.0014715021960006302 0.0 0.0 2.1178378958557307 3.0295633447071794E-4 86-87 0.0015364214105300696 0.0 0.0 2.4490990078612844 3.462358108236777E-4 88-89 0.0015580611487065496 0.0 0.0 2.8495856422933965 3.895152871766374E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7260 0.0 23.372 1 TACATGG 7510 0.0 22.263548 2 GTATCAA 12660 0.0 21.512218 1 GAGTACT 6775 0.0 21.421041 12-13 ACATGGG 7600 0.0 21.187405 3 GGTATCA 10130 0.0 20.55081 1 GTACTTT 7280 0.0 19.834215 14-15 GGATCGT 250 2.1754931E-7 18.999092 6 AGTACTT 7375 0.0 18.840935 12-13 AGAGTAC 11095 0.0 18.753633 10-11 ATCAACG 14770 0.0 18.234514 3 TATCAAC 14800 0.0 18.229647 2 CATGGGA 4145 0.0 18.220665 4 TCAACGC 14835 0.0 18.15462 4 CAACGCA 14865 0.0 18.086025 5 AACGCAG 15125 0.0 17.80576 6 CCTATAC 555 0.0 17.117039 3 GCACCGT 420 4.7293724E-11 16.963474 6 AGGACCG 820 0.0 16.798704 5 GGACCGA 570 0.0 16.665869 6 >>END_MODULE