FastQCFastQC Report
Thu 26 May 2016
SRR937247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483207
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56300.37958289031807424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40890.27568640115641313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.1932973617303586No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26290.17725105126931037No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24130.16268801320382117No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16010.10794177751318595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93000.027.9518181
GGTATCA66850.025.0945221
TATCAAC109800.023.4491122
GTACATG72850.023.288651
ATCAACG110250.023.2672273
TCAACGC111600.023.0306724
CAACGCA111950.022.8313545
AACGCAG113550.022.5499616
GAGTACT52250.022.09198612-13
TACATGG75400.022.0508922
ACATGGG76000.020.8142193
GTACTTT56000.020.6536314-15
AGTACTT54750.020.34574112-13
ACGCAGA126650.019.9194587
CGCAGAG126750.019.7544778
GTACCTG12350.019.6249641
GTGTAGC8250.019.585321
AGAGTAC95650.019.44489510-11
GTATTAC2201.3390036E-619.4413091
ATGGGAG21500.019.0034965
Produced by FastQC (version 0.11.5)