FastQCFastQC Report
Thu 26 May 2016
SRR937247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483207
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73410.49494102980905563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.39893285293286773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41590.280405904233192No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29630.19976982309279825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103150.028.7410411
GGTATCA72500.025.2295741
GTACATG67900.025.189521
ATCAACG122250.023.9721153
TCAACGC122700.023.9229054
TACATGG69450.023.8683972
TATCAAC123900.023.7678782
CAACGCA123600.023.748715
ACATGGG68650.023.5930373
AACGCAG126350.023.3070056
GAGTACT65150.022.8245612-13
GGATCGC1252.2535573E-422.8041448
AGTACTT68600.021.95369712-13
GGGCGTA2457.506969E-921.3304069
GTACTTT70000.021.068514-15
ACGCAGA138750.021.0236427
GTGTAGG5900.020.9367121
GGACCGA2509.500582E-920.8988656
CGCAGAG139200.020.8874178
AGAGTAC107850.020.81393410-11
Produced by FastQC (version 0.11.5)