##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937247_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1483207 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21930586897176 33.0 31.0 34.0 31.0 34.0 2 32.423340774416516 34.0 31.0 34.0 31.0 34.0 3 32.49573390632595 34.0 31.0 34.0 31.0 34.0 4 36.11916408161504 37.0 35.0 37.0 35.0 37.0 5 36.04947859604223 37.0 35.0 37.0 35.0 37.0 6 36.088518999708064 37.0 35.0 37.0 35.0 37.0 7 36.030767114772246 37.0 35.0 37.0 35.0 37.0 8 36.00631941461981 37.0 35.0 37.0 35.0 37.0 9 37.70510656975055 39.0 37.0 39.0 35.0 39.0 10-11 37.628049557479166 39.0 37.0 39.0 35.0 39.0 12-13 37.515319844094584 39.0 37.0 39.0 35.0 39.0 14-15 38.7441766388643 40.0 38.0 41.0 35.0 41.0 16-17 38.703978945622566 40.0 38.0 41.0 35.0 41.0 18-19 38.69297508709168 40.0 38.0 41.0 34.5 41.0 20-21 38.590606031390095 40.0 38.0 41.0 34.0 41.0 22-23 38.5377030987583 40.0 38.0 41.0 34.0 41.0 24-25 38.569490974624586 40.0 38.0 41.0 34.0 41.0 26-27 38.42219764334985 40.0 38.0 41.0 34.0 41.0 28-29 38.275823603853 40.0 38.0 41.0 34.0 41.0 30-31 38.313577605823056 40.0 38.0 41.0 34.0 41.0 32-33 38.21357066141139 40.0 38.0 41.0 34.0 41.0 34-35 38.08336665077768 40.0 37.5 41.0 33.5 41.0 36-37 37.91784828415723 40.0 37.0 41.0 33.0 41.0 38-39 37.80426703757466 40.0 37.0 41.0 33.0 41.0 40-41 37.621139193652674 40.0 37.0 41.0 33.0 41.0 42-43 37.41846856170447 40.0 37.0 41.0 33.0 41.0 44-45 37.1256490159499 40.0 36.5 41.0 31.5 41.0 46-47 36.77381478107911 39.5 36.0 41.0 31.0 41.0 48-49 36.44542872303057 39.0 36.0 41.0 30.0 41.0 50-51 36.19761503283088 39.0 35.5 40.5 30.0 41.0 52-53 35.963137984111455 39.0 35.0 40.0 29.0 41.0 54-55 35.74651077024313 39.0 35.0 40.0 29.0 41.0 56-57 35.44149906250443 38.0 35.0 40.0 28.0 41.0 58-59 35.16912575250791 38.0 34.5 40.0 28.0 41.0 60-61 34.82093025450932 38.0 34.0 40.0 27.0 41.0 62-63 34.44176032071046 37.0 34.0 40.0 26.0 41.0 64-65 34.024796943380125 37.0 33.5 39.5 26.0 41.0 66-67 33.57260011583009 36.0 33.0 39.0 25.0 40.0 68-69 33.12270067495636 36.0 32.0 39.0 24.0 40.0 70-71 32.67821214436016 35.0 32.0 38.0 23.0 40.0 72-73 32.196908792906186 35.0 31.0 37.5 22.0 39.5 74-75 31.709702354425243 35.0 31.0 37.0 20.5 39.0 76-77 30.399774610017346 33.5 29.5 35.5 20.0 38.0 78-79 31.054768484776567 34.0 31.0 36.0 20.0 38.0 80-81 30.86553798626894 34.0 31.0 35.5 19.5 37.0 82-83 30.463181470961235 34.0 30.5 35.0 18.0 37.0 84-85 30.020782331798596 34.0 30.0 35.0 14.0 36.5 86-87 29.639627846955953 34.0 30.0 35.0 7.5 36.0 88-89 29.184908107904022 34.0 29.0 35.0 4.5 36.0 90-91 28.6157124393291 33.5 29.0 35.0 2.0 35.0 92-93 28.111571749593956 33.0 27.5 35.0 2.0 35.0 94-95 27.597665396670862 33.0 27.0 35.0 2.0 35.0 96-97 26.920275457168152 33.0 25.5 34.5 2.0 35.0 98-99 25.926308330529725 32.0 24.5 34.0 2.0 35.0 100-101 22.928944510105467 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23833553052970302 1101 2 0.2698204253068681 1101 3 0.17723570415149936 1101 4 0.13448158926768627 1101 5 0.17911166274772938 1101 6 0.1343073727192916 1101 7 0.16405947337319304 1101 8 0.1420909283604388 1101 9 0.26055115172302123 1101 10-11 0.25114835443885397 1101 12-13 0.23699225668306667 1101 14-15 0.4004440807773051 1101 16-17 0.32717349753125546 1101 18-19 0.30184316306809933 1101 20-21 0.42504701330956607 1101 22-23 0.3473795356038991 1101 24-25 0.36207694565256077 1101 26-27 0.1879940657837338 1101 28-29 0.18298695595570535 1101 30-31 0.3576560527981272 1101 32-33 0.3955651822492925 1101 34-35 0.3638655032287872 1101 36-37 0.3753185557415222 1101 38-39 0.634888123957019 1101 40-41 0.6764199682823033 1101 42-43 0.5426123775732208 1101 44-45 0.5860176641560315 1101 46-47 0.7904329997119746 1101 48-49 0.6254899438457571 1101 50-51 0.7074699140607734 1101 52-53 0.7432243755780519 1101 54-55 0.6658028793221433 1101 56-57 0.7899457972497785 1101 58-59 0.908208203707737 1101 60-61 0.8196249212075557 1101 62-63 0.8887929755600013 1101 64-65 0.7507223577084261 1101 66-67 0.8099782136689058 1101 68-69 0.8583647126482745 1101 70-71 0.925010253696513 1101 72-73 0.9053900539973085 1101 74-75 0.798721664082219 1101 76-77 0.7764892091508919 1101 78-79 0.8653659837502694 1101 80-81 0.7433021457923381 1101 82-83 0.7419653979737326 1101 84-85 0.894236394108642 1101 86-87 0.9584943908554067 1101 88-89 1.2857703216406655 1101 90-91 1.3860418752726353 1101 92-93 1.5004597068761747 1101 94-95 1.55294054151965 1101 96-97 1.7576030003773617 1101 98-99 2.2966112844120445 1101 100-101 2.8969237755601718 1102 1 0.2512530168768379 1102 2 0.27914783830212997 1102 3 0.23187005465110389 1102 4 0.12743064284386207 1102 5 0.16208094781691784 1102 6 0.17803510155628288 1102 7 0.16238537663141273 1102 8 0.13087126077370215 1102 9 0.2853588762900543 1102 10-11 0.2282794812855684 1102 12-13 0.22947047055062342 1102 14-15 0.2713590220907207 1102 16-17 0.39589576183181663 1102 18-19 0.3784555402007541 1102 20-21 0.37947044964728605 1102 22-23 0.34708999989462797 1102 24-25 0.22352214185907116 1102 26-27 0.26891262470500266 1102 28-29 0.2975619759923376 1102 30-31 0.2915093219464566 1102 32-33 0.30666522308505506 1102 34-35 0.35767532719447104 1102 36-37 0.39984797019737783 1102 38-39 0.26765094274282575 1102 40-41 0.3527554755691966 1102 42-43 0.4122702637041371 1102 44-45 0.3630535640423389 1102 46-47 0.4682263521245815 1102 48-49 0.5769447389976534 1102 50-51 0.519079385246151 1102 52-53 0.43407055692610896 1102 54-55 0.4252315345039648 1102 56-57 0.5844599533643873 1102 58-59 0.5642103403838448 1102 60-61 0.49281874365502176 1102 62-63 0.4848597377925117 1102 64-65 0.36561169449161923 1102 66-67 0.5684651894334749 1102 68-69 0.40809093513708206 1102 70-71 0.40914551834104174 1102 72-73 0.49580229184782354 1102 74-75 0.353400493366717 1102 76-77 0.33902426266945795 1102 78-79 0.27905574888533735 1102 80-81 0.3682314033439873 1102 82-83 0.22872935880529255 1102 84-85 0.3639380812524955 1102 86-87 0.6575858799034577 1102 88-89 0.5139829183714326 1102 90-91 0.9592941979124205 1102 92-93 0.9443306574005135 1102 94-95 1.0567547032258737 1102 96-97 1.366159193603373 1102 98-99 1.882825572183851 1102 100-101 2.652521324736764 1103 1 0.2432914486290514 1103 2 0.19491478932148 1103 3 0.18667040459359185 1103 4 0.10175271294365018 1103 5 0.1622035364709049 1103 6 0.10838257135575446 1103 7 0.26030651572686025 1103 8 0.21754897018168862 1103 9 0.2327382195579517 1103 10-11 0.2567982881841715 1103 12-13 0.2604563218116738 1103 14-15 0.278121271646917 1103 16-17 0.33881484453397803 1103 18-19 0.446374047614853 1103 20-21 0.45829746450478126 1103 22-23 0.2992260339717703 1103 24-25 0.35449933241999787 1103 26-27 0.32756669333037536 1103 28-29 0.35149136074373644 1103 30-31 0.36178400002572175 1103 32-33 0.3901882620117618 1103 34-35 0.5257790982808714 1103 36-37 0.422912209580808 1103 38-39 0.40345176672267513 1103 40-41 0.4382794709384399 1103 42-43 0.5098684047078663 1103 44-45 0.395841217482122 1103 46-47 0.4746879875241703 1103 48-49 0.5047709827599576 1103 50-51 0.32359378564746777 1103 52-53 0.39321255349780415 1103 54-55 0.3689330089567804 1103 56-57 0.4961116074535141 1103 58-59 0.4002574294890593 1103 60-61 0.48249640243766123 1103 62-63 0.5134161999090665 1103 64-65 0.671521011522195 1103 66-67 0.7418260473364953 1103 68-69 0.8512614710168549 1103 70-71 0.8266197151029431 1103 72-73 0.6586479301393311 1103 74-75 0.6849239356226455 1103 76-77 0.770409342919006 1103 78-79 0.8251923862827439 1103 80-81 0.8407562735694647 1103 82-83 0.7108299509545155 1103 84-85 0.8801155996648511 1103 86-87 1.0062315681508878 1103 88-89 1.1574624780731497 1103 90-91 1.2918977173364468 1103 92-93 1.215639640638507 1103 94-95 1.4900955536564204 1103 96-97 1.6282813496407371 1103 98-99 1.87194174982778 1103 100-101 2.8492761143758862 1104 1 0.18240204415907613 1104 2 0.16636762414839268 1104 3 0.2038847633012395 1104 4 0.12832595198253216 1104 5 -0.033603597374536776 1104 6 0.05533889346571641 1104 7 0.04052052702675013 1104 8 0.06397361622195064 1104 9 0.18409544614703321 1104 10-11 0.10798607730970389 1104 12-13 0.04103643180381056 1104 14-15 0.20051566273865262 1104 16-17 0.16188210958171112 1104 18-19 0.14943570537962358 1104 20-21 0.18375635763525366 1104 22-23 0.018593508073642795 1104 24-25 4.814906957122389E-4 1104 26-27 0.038385092532358556 1104 28-29 0.09779187454151383 1104 30-31 0.1179506907332808 1104 32-33 0.16588170616342524 1104 34-35 0.12740683564060618 1104 36-37 0.20914226664913116 1104 38-39 0.20319212514181828 1104 40-41 0.1371523257971745 1104 42-43 0.009310927010027115 1104 44-45 -0.020562116819625942 1104 46-47 0.0880138173918894 1104 48-49 0.16490177873003375 1104 50-51 0.13999651046193407 1104 52-53 0.20899421077498914 1104 54-55 0.3353628559551609 1104 56-57 0.3825570166937453 1104 58-59 0.34049557648520334 1104 60-61 0.292156166322286 1104 62-63 0.30597984561817526 1104 64-65 0.22174215382325002 1104 66-67 0.3080882853331488 1104 68-69 0.41745788438738174 1104 70-71 0.364789871761495 1104 72-73 0.4284608378609249 1104 74-75 0.34975672278765657 1104 76-77 0.5840764856629583 1104 78-79 0.4928816371619291 1104 80-81 0.5557057775484004 1104 82-83 0.39816135153596477 1104 84-85 0.4504325533661664 1104 86-87 0.5301905130877778 1104 88-89 0.5476409968971652 1104 90-91 0.6752260054695256 1104 92-93 0.6512304471323915 1104 94-95 0.7498929600848854 1104 96-97 0.956346613538571 1104 98-99 1.305031626853566 1104 100-101 2.417384286178166 1105 1 0.17284841654325334 1105 2 0.17393711472939088 1105 3 0.07986918478618321 1105 4 0.049251934553140586 1105 5 0.07948831703325254 1105 6 0.04673525133514289 1105 7 0.01265064932082538 1105 8 0.12749210840249248 1105 9 0.2927776043052006 1105 10-11 0.06976786509821409 1105 12-13 0.143633500408896 1105 14-15 0.1925265108091594 1105 16-17 0.18878485686335722 1105 18-19 0.18393560893934335 1105 20-21 0.3963748379312335 1105 22-23 0.2622801765197451 1105 24-25 0.19214982964336258 1105 26-27 0.15329281184632038 1105 28-29 0.2866046069966899 1105 30-31 0.23567446628265287 1105 32-33 0.24714969130188535 1105 34-35 0.19525072317760106 1105 36-37 0.11993422645203822 1105 38-39 0.26677666430339997 1105 40-41 0.03135382948625676 1105 42-43 0.03754104326440455 1105 44-45 0.09306382347312336 1105 46-47 0.09333322955428258 1105 48-49 0.23986646600049966 1105 50-51 0.3396777704590832 1105 52-53 0.3467113231776011 1105 54-55 0.26624225494711595 1105 56-57 0.3549478199925957 1105 58-59 0.25395143118964114 1105 60-61 0.600819815739456 1105 62-63 0.5602940383155968 1105 64-65 0.43790457094792856 1105 66-67 0.4243023321037569 1105 68-69 0.39744993537019724 1105 70-71 0.5877490882377785 1105 72-73 0.5435287064917276 1105 74-75 0.6962179489235574 1105 76-77 0.5890107211837652 1105 78-79 0.6585533778213453 1105 80-81 0.6519114856441242 1105 82-83 0.667627968586217 1105 84-85 0.8790909034385486 1105 86-87 0.927565389127917 1105 88-89 1.0606643315488427 1105 90-91 1.2908216654620048 1105 92-93 1.4093414645091755 1105 94-95 1.4520627690162335 1105 96-97 1.7253606431642474 1105 98-99 2.0151670134232305 1105 100-101 2.5804725962521786 1106 1 -0.14826187163208715 1106 2 0.12404086420568206 1106 3 0.010558974844897762 1106 4 0.11294041210652495 1106 5 0.04695565828662751 1106 6 0.08150492749778238 1106 7 0.027616784300377617 1106 8 -0.02886242291793195 1106 9 -0.04849641940726457 1106 10-11 -0.13328306447724003 1106 12-13 -0.2510384427542576 1106 14-15 -0.012554334356906338 1106 16-17 -0.08565762621405781 1106 18-19 0.1019089895043308 1106 20-21 0.17562792112739345 1106 22-23 0.031292938201737286 1106 24-25 0.1300647530871757 1106 26-27 0.15218720977173206 1106 28-29 0.06237468624341602 1106 30-31 0.09971579133719644 1106 32-33 0.19888920764068985 1106 34-35 -0.22849569523162216 1106 36-37 0.30129526073007185 1106 38-39 0.38515352608526854 1106 40-41 0.2849795013758012 1106 42-43 0.29006548147835787 1106 44-45 0.2247722447535878 1106 46-47 0.17040687877581462 1106 48-49 0.11206994602598996 1106 50-51 0.23709306819526432 1106 52-53 0.10358092845303446 1106 54-55 -0.03207432292852985 1106 56-57 -0.018187121838202813 1106 58-59 0.1813210394435245 1106 60-61 0.1089889204360901 1106 62-63 0.1506742280232558 1106 64-65 0.22063946671197243 1106 66-67 0.15132643970934367 1106 68-69 0.26847494048320897 1106 70-71 0.2599865226373339 1106 72-73 0.35007991504902947 1106 74-75 0.3645133003513301 1106 76-77 0.2811930740431805 1106 78-79 0.26882983790907033 1106 80-81 0.273056323884294 1106 82-83 0.16301057084150372 1106 84-85 0.545898616442674 1106 86-87 0.5193148733589901 1106 88-89 0.6140656265805831 1106 90-91 0.5314650512095049 1106 92-93 0.6175919802781031 1106 94-95 0.4106078538723814 1106 96-97 0.756332592085748 1106 98-99 1.0217712384593227 1106 100-101 1.61445079794116 1107 1 0.06751366420959215 1107 2 0.09561101216312551 1107 3 0.0927684106214457 1107 4 0.09784766729433869 1107 5 -0.005164832423382393 1107 6 0.12938133495459425 1107 7 0.12110913828158232 1107 8 0.11735721788978282 1107 9 0.022128057158383285 1107 10-11 0.04103763405559846 1107 12-13 0.10256860150889935 1107 14-15 0.11000374746944885 1107 16-17 0.10615159553599796 1107 18-19 0.1215251764207963 1107 20-21 0.15744594432910475 1107 22-23 0.25096902927155185 1107 24-25 0.09284023464677915 1107 26-27 0.07440215130217354 1107 28-29 0.1510115081051424 1107 30-31 0.1111949004907089 1107 32-33 0.14619507165632228 1107 34-35 0.16117171070528258 1107 36-37 0.3059741822226769 1107 38-39 0.34127286136241963 1107 40-41 0.26376994152593625 1107 42-43 0.330170827487855 1107 44-45 0.2758222152861691 1107 46-47 0.3474678530616089 1107 48-49 0.18920672136021466 1107 50-51 0.2349262438520796 1107 52-53 0.2315367816122489 1107 54-55 0.3527693446790252 1107 56-57 0.34192640525630935 1107 58-59 0.47935544652811757 1107 60-61 0.5626316357808534 1107 62-63 0.35589538872923043 1107 64-65 0.6269598395523204 1107 66-67 0.5222389990319058 1107 68-69 0.5125203900939326 1107 70-71 0.5850045520427045 1107 72-73 0.6579876510209814 1107 74-75 0.5171316352872815 1107 76-77 0.39448636787983915 1107 78-79 0.4120255790921128 1107 80-81 0.413287875731438 1107 82-83 0.4532196343980637 1107 84-85 0.6901689489591938 1107 86-87 0.7351981979037951 1107 88-89 0.9337839801275294 1107 90-91 0.9736028734124282 1107 92-93 0.8990387528206725 1107 94-95 0.9862805639440317 1107 96-97 1.0066330821753908 1107 98-99 1.4877082634911147 1107 100-101 1.8890027769107327 1108 1 0.09015706623059572 1108 2 0.10966019979934316 1108 3 0.06128312233136768 1108 4 0.04556413252120706 1108 5 0.12587836155323373 1108 6 0.05377949227577261 1108 7 0.056225296395311375 1108 8 0.06304277366549371 1108 9 0.021719398549315372 1108 10-11 -0.01428916899571675 1108 12-13 0.059092898120745474 1108 14-15 0.06900476292418034 1108 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94-95 0.30495360694929374 2316 96-97 0.40218276322110214 2316 98-99 0.7983636651097861 2316 100-101 1.439146903866078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 14.0 5 47.0 6 53.0 7 135.0 8 270.0 9 572.0 10 1082.0 11 1810.0 12 2831.0 13 4118.0 14 5238.0 15 7308.0 16 10247.0 17 14360.0 18 12978.0 19 7977.0 20 6978.0 21 7424.0 22 7762.0 23 8608.0 24 10017.0 25 11878.0 26 14560.0 27 18285.0 28 22701.0 29 28718.0 30 35712.0 31 45725.0 32 58640.0 33 77529.0 34 106428.0 35 152537.0 36 228072.0 37 325822.0 38 229596.0 39 17170.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88585805442012 19.89517989396576 15.333346823694471 21.88561522791965 2 15.718315594731951 21.037603677439275 39.469365684874525 23.77471504295425 3 19.70068911487068 24.814742648868297 29.965810571282365 25.518757664978658 4 12.986993723735122 17.005920279502458 37.54958006535837 32.45750593140404 5 13.667675076675792 37.80162123203116 33.98236102786353 14.548342663429507 6 31.71627426245966 36.81724803078734 17.110558404861898 14.355919301891104 7 28.617718228136734 33.08216587435199 20.3695775437953 17.93053835371597 8 26.781224738017013 34.77316382676187 19.511504462964375 18.93410697225674 9 26.474457038026383 16.902900269483627 20.269726342985166 36.352916349504824 10-11 25.009894101093106 27.16542599920308 26.96201541659391 20.86266448310991 12-13 26.180401765592126 24.22703090123344 27.8689607812135 21.723606551960938 14-15 22.845125461247147 25.526881952417966 26.59052310297888 25.037469483356002 16-17 21.847085403453463 29.179709912372314 26.99393948383469 21.979265200339533 18-19 21.817116558915917 27.408783804283555 29.781244290244047 20.992855346556482 20-21 22.599827670600035 26.19491156319486 30.030791407262374 21.174469358942723 22-23 22.230694911060365 26.23414151997341 29.962574088270134 21.57258948069609 24-25 22.1634269525427 26.34632252949184 30.07917977733384 21.41107074063162 26-27 21.993944097180318 26.574436970268632 30.047108208640754 21.3845107239103 28-29 21.693156296796232 26.86923654173288 30.06161173549161 21.375995425979276 30-31 21.82171164586935 26.490354664631433 30.040540613428767 21.647393076070454 32-33 21.751173977973377 26.602863393799236 29.90442993382574 21.74153269440165 34-35 22.330834468823298 26.498492792981693 29.783266934419807 21.3874058037752 36-37 22.229028045309924 26.303678448119516 29.844215945582782 21.623077560987777 38-39 21.95816227943908 26.69162160103074 29.903411998460093 21.446804121070087 40-41 22.136829181631423 26.59804059716547 29.824832272231728 21.440297948971384 42-43 21.908034049376248 26.70824226450131 29.47073373917752 21.912989946944926 44-45 22.142593717532346 26.7464352581939 29.179608780163523 21.93136224411023 46-47 22.283976545418138 27.020806940636067 28.858345463579933 21.836871050365865 48-49 22.347588704745867 27.19970981798225 28.570556908105203 21.882144569166677 50-51 22.224746781804562 27.29760579608915 28.557611985380326 21.92003543672596 52-53 22.51317584126828 27.551515061619853 28.08525714886729 21.85005194824458 54-55 22.233781259122967 27.308865182000897 28.263249836334374 22.194103722541765 56-57 22.37071426982208 27.475699615765027 28.168657510381223 21.98492860403167 58-59 22.63814154059413 27.184809672554135 28.158476868029886 22.01857191882185 60-61 22.479094286906683 27.160571653181247 28.76523641002234 21.595097649889734 62-63 22.35460053788851 27.213362666168646 28.616605773840064 21.815431022102782 64-65 22.215038089760903 27.303471464198857 28.514563375172852 21.966927070867385 66-67 22.165752993344825 27.447079200677994 28.19795214019351 22.18921566578367 68-69 22.11892878067593 27.299796993946224 28.415453810560493 22.165820414817354 70-71 22.28488673529723 27.28941408717731 28.277239791883396 22.14845938564206 72-73 22.289905980629783 27.19873517146989 28.312067448532062 22.19929139936826 74-75 22.105478196907107 27.63302762190308 28.036545134967678 22.22494904622214 76-77 22.24264718276006 27.252062591398236 28.231460612038646 22.273829613803066 78-79 22.13841358623577 27.305392976165837 28.23567445407148 22.320518983526913 80-81 22.165719282608563 27.579427551245377 28.08006569548283 22.174787470663233 82-83 22.18125233026093 27.47671761721086 28.185420336932975 22.156609715595234 84-85 22.064703082376962 27.427478044182667 28.053823946235383 22.453994927204988 86-87 22.080903070171594 27.3065054304625 28.136531178722862 22.47606032064304 88-89 22.116400475456224 27.264636696024226 28.03735419263798 22.581608635881572 90-91 22.159514820017957 27.1675774411132 28.112908386171064 22.559999352697783 92-93 22.231114458247635 26.992198916997566 28.125329736584924 22.651356888169868 94-95 22.025752305645806 26.979207892087885 28.136059228415185 22.85898057385112 96-97 21.800092637103248 27.06965379748073 28.28802722748747 22.842226337928555 98-99 21.778167625291513 27.039524649727177 27.99617179330381 23.1861359316775 100-101 21.587518859098303 26.987281171244824 28.431243309761218 22.99395665989565 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 300.0 1 214.5 2 193.5 3 271.0 4 238.0 5 241.5 6 452.0 7 1040.5 8 1754.5 9 2132.0 10 2009.0 11 1844.5 12 1936.0 13 2024.5 14 2174.5 15 2297.0 16 2446.5 17 2608.0 18 2696.0 19 2769.0 20 2928.5 21 3276.0 22 3813.5 23 4545.0 24 5389.5 25 6407.0 26 7867.0 27 9710.0 28 11648.0 29 14102.5 30 17006.5 31 19796.0 32 22628.5 33 25656.5 34 29331.0 35 32190.5 36 35582.0 37 39513.0 38 42137.0 39 43657.0 40 45038.0 41 47364.5 42 49839.0 43 52502.5 44 55115.5 45 56898.0 46 56963.0 47 59372.0 48 65742.0 49 64693.0 50 57965.0 51 54091.5 52 50397.5 53 47804.0 54 45638.0 55 41804.0 56 38181.5 57 34229.5 58 30454.5 59 27153.0 60 22784.5 61 18218.0 62 14115.0 63 10661.5 64 7914.0 65 6080.5 66 4502.0 67 3333.0 68 2340.0 69 1487.0 70 1033.5 71 747.0 72 566.0 73 439.0 74 354.0 75 268.5 76 193.0 77 106.5 78 50.0 79 32.0 80 23.5 81 14.0 82 7.5 83 4.0 84 2.0 85 1.5 86 1.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04497012217445036 2 6.742147252541284E-5 3 0.0 4 0.0 5 9.439006153557797E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005292585593244908 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.742147252541284E-5 22-23 6.742147252541284E-4 24-25 0.0 26-27 0.0017529582856607336 28-29 0.0032362306812198163 30-31 2.0226441757623852E-4 32-33 1.348429450508257E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00846139480193931 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.348429450508257E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.696858901016514E-4 84-85 6.742147252541284E-5 86-87 0.0 88-89 0.0 90-91 0.008663659219515549 92-93 0.001045032824143899 94-95 0.0 96-97 0.0 98-99 4.0452883515247704E-4 100-101 0.011427939593057476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1483207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.87234167578191 #Duplication Level Percentage of deduplicated Percentage of total 1 77.93892479755009 45.10508085726891 2 12.783936456102918 14.796726770981453 3 3.9883784180630406 6.924503956273766 4 1.7295511660095348 4.0037270410020325 5 0.9185003217163223 2.657788222384131 6 0.5770962306740111 2.003874614482333 7 0.3831315528579217 1.5520904093636583 8 0.27301596841522513 1.2640058725656316 9 0.1862066735098952 0.9698594608507881 >10 1.1060433961459262 11.624618014421246 >50 0.07770725409785531 3.0985382969475483 >100 0.03505404774320438 3.6940825375503215 >500 0.0015189677372671366 0.5813305941204735 >1k 7.010620325848322E-4 0.8272651492927617 >5k 2.336873441949441E-4 0.8965082024949677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7341 0.49494102980905563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5917 0.39893285293286773 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4159 0.280405904233192 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2963 0.19976982309279825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.742147252541284E-5 0.0 0.0 0.0 0.0 6 6.742147252541284E-5 0.0 0.0 0.0 0.0 7 6.742147252541284E-5 0.0 0.0 0.0 0.0 8 6.742147252541284E-5 0.0 0.0 0.0 0.0 9 6.742147252541284E-5 0.0 0.0 0.0 0.0 10-11 6.742147252541284E-5 0.0 0.0 0.0 0.0 12-13 6.742147252541284E-5 0.0 0.0 0.0 0.0 14-15 6.742147252541284E-5 0.0 0.0 0.0 0.0 16-17 1.0113220878811926E-4 0.0 0.0 0.0 0.0 18-19 1.348429450508257E-4 0.0 0.0 0.0 0.0 20-21 1.348429450508257E-4 0.0 0.0 0.0 0.0 22-23 1.348429450508257E-4 0.0 0.0 6.742147252541284E-5 3.371073626270642E-5 24-25 1.348429450508257E-4 0.0 0.0 2.0226441757623852E-4 6.742147252541284E-5 26-27 1.348429450508257E-4 0.0 0.0 3.3710736262706424E-4 6.742147252541284E-5 28-29 1.348429450508257E-4 0.0 0.0 8.427684065676604E-4 6.742147252541284E-5 30-31 1.348429450508257E-4 0.0 0.0 0.002292330065864037 6.742147252541284E-5 32-33 1.348429450508257E-4 0.0 0.0 0.003809313197685825 6.742147252541284E-5 34-35 1.348429450508257E-4 0.0 0.0 0.0051577426481940825 6.742147252541284E-5 36-37 1.348429450508257E-4 0.0 0.0 0.007382651241532706 6.742147252541284E-5 38-39 1.348429450508257E-4 0.0 0.0 0.01024806382386275 6.742147252541284E-5 40-41 1.348429450508257E-4 0.0 0.0 0.014765302483065412 6.742147252541284E-5 42-43 1.348429450508257E-4 0.0 0.0 0.018776880098327474 6.742147252541284E-5 44-45 1.348429450508257E-4 0.0 0.0 0.020664681329039036 6.742147252541284E-5 46-47 2.0226441757623852E-4 0.0 0.0 0.02336154023005555 6.742147252541284E-5 48-49 2.0226441757623852E-4 0.0 0.0 0.027305696372792197 6.742147252541284E-5 50-51 2.0226441757623852E-4 0.0 0.0 0.03283425711987605 6.742147252541284E-5 52-53 2.0226441757623852E-4 0.0 0.0 0.04577917984475532 1.348429450508257E-4 54-55 2.0226441757623852E-4 0.0 0.0 0.055420450415889354 2.0226441757623852E-4 56-57 2.0226441757623852E-4 0.0 0.0 0.08013042009645316 2.0226441757623852E-4 58-59 2.0226441757623852E-4 0.0 0.0 0.12563991405110683 2.0226441757623852E-4 60-61 2.0226441757623852E-4 0.0 0.0 0.16926160677504892 2.0226441757623852E-4 62-63 2.0226441757623852E-4 0.0 0.0 0.19393786571935004 3.371073626270642E-4 64-65 2.0226441757623852E-4 0.0 0.0 0.23971704556410534 3.371073626270642E-4 66-67 2.696858901016514E-4 0.0 0.0 0.329556157704218 3.371073626270642E-4 68-69 3.371073626270642E-4 0.0 0.0 0.45549946838168914 3.371073626270642E-4 70-71 4.7195030767788985E-4 0.0 0.0 0.5690709388507471 3.371073626270642E-4 72-73 5.056610439405963E-4 0.0 0.0 0.7020935041433867 4.0452883515247704E-4 74-75 5.393717802033028E-4 0.0 0.0 0.8570280480067853 4.0452883515247704E-4 76-77 5.393717802033028E-4 0.0 0.0 0.9713411546736228 4.0452883515247704E-4 78-79 5.393717802033028E-4 0.0 0.0 1.1192975761306414 4.0452883515247704E-4 80-81 6.067932527287156E-4 0.0 0.0 1.31508953234444 4.3823957141518344E-4 82-83 6.067932527287156E-4 0.0 0.0 1.4947003351521397 4.7195030767788985E-4 84-85 6.742147252541284E-4 0.0 0.0 1.7082915601126478 4.7195030767788985E-4 86-87 7.416361977795412E-4 0.0 0.0 2.005316857323354 4.7195030767788985E-4 88-89 7.416361977795412E-4 0.0 0.0 2.373370675839583 4.7195030767788985E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10315 0.0 28.741041 1 GGTATCA 7250 0.0 25.229574 1 GTACATG 6790 0.0 25.18952 1 ATCAACG 12225 0.0 23.972115 3 TCAACGC 12270 0.0 23.922905 4 TACATGG 6945 0.0 23.868397 2 TATCAAC 12390 0.0 23.767878 2 CAACGCA 12360 0.0 23.74871 5 ACATGGG 6865 0.0 23.593037 3 AACGCAG 12635 0.0 23.307005 6 GAGTACT 6515 0.0 22.82456 12-13 GGATCGC 125 2.2535573E-4 22.804144 8 AGTACTT 6860 0.0 21.953697 12-13 GGGCGTA 245 7.506969E-9 21.330406 9 GTACTTT 7000 0.0 21.0685 14-15 ACGCAGA 13875 0.0 21.023642 7 GTGTAGG 590 0.0 20.936712 1 GGACCGA 250 9.500582E-9 20.898865 6 CGCAGAG 13920 0.0 20.887417 8 AGAGTAC 10785 0.0 20.813934 10-11 >>END_MODULE