FastQCFastQC Report
Thu 26 May 2016
SRR937246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461926
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57730.3948900286334603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.28106757797590304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.20575596849635341No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25100.17169131679715663No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18670.12770824241445874No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15920.108897440773336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89250.028.6664811
GTACATG74550.026.4728551
TACATGG77300.025.147272
TCAACGC104900.024.1556054
ATCAACG104950.024.1374743
TATCAAC106050.023.887112
CAACGCA105750.023.8723585
ACATGGG77800.023.7027763
GGTATCA62400.023.6253071
AACGCAG107800.023.4880546
CATGGGG31000.021.7767414
GAGTACT51400.021.1642812-13
AGTACTT53850.020.95120812-13
ACGCAGA120800.020.7251247
TATAACG1150.003711180520.664232
CATGGGA45900.020.611364
CGCAGAG121300.020.5613678
AGAGTAC95950.020.00207110-11
GTACTTT54750.019.91199714-15
GTGTAGC7650.019.8924791
Produced by FastQC (version 0.11.5)