##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937246_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1461926 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58433190188833 31.0 31.0 34.0 30.0 34.0 2 31.688411725354086 33.0 31.0 34.0 30.0 34.0 3 31.72875097645161 33.0 31.0 34.0 30.0 34.0 4 35.357194550202955 37.0 35.0 37.0 33.0 37.0 5 35.36332139930475 37.0 35.0 37.0 33.0 37.0 6 35.404558780677 37.0 35.0 37.0 33.0 37.0 7 35.338706610320905 37.0 35.0 37.0 33.0 37.0 8 35.32786064410921 37.0 35.0 37.0 33.0 37.0 9 36.92459057435192 39.0 37.0 39.0 33.0 39.0 10-11 36.79632553220888 39.0 37.0 39.0 33.0 39.0 12-13 36.65554446668299 39.0 37.0 39.0 32.0 39.0 14-15 37.795945896030304 40.0 37.5 41.0 33.0 41.0 16-17 37.757555785997376 40.0 37.5 41.0 32.5 41.0 18-19 37.72874584623298 40.0 37.5 41.0 32.0 41.0 20-21 37.6597830533146 40.0 37.0 41.0 32.0 41.0 22-23 37.5110327061698 40.0 37.0 41.0 32.0 41.0 24-25 37.34277213757741 40.0 37.0 41.0 32.0 41.0 26-27 37.17672987552038 39.0 36.5 41.0 31.0 41.0 28-29 37.24992817693919 39.0 37.0 41.0 31.0 41.0 30-31 37.11034108429565 39.0 36.5 41.0 31.0 41.0 32-33 36.92495174174343 39.0 36.0 41.0 31.0 41.0 34-35 36.796841632203 39.0 36.0 41.0 30.0 41.0 36-37 36.796005064551835 39.0 36.0 41.0 30.0 41.0 38-39 36.71252922514546 39.0 36.0 41.0 30.0 41.0 40-41 36.5473662825615 39.0 35.5 41.0 30.0 41.0 42-43 36.30896604889714 39.0 35.0 41.0 30.0 41.0 44-45 36.085951682916914 39.0 35.0 41.0 29.5 41.0 46-47 35.75863621004073 39.0 35.0 40.5 27.5 41.0 48-49 35.45079709916918 39.0 35.0 40.0 27.0 41.0 50-51 34.573054655297184 38.0 34.0 39.5 25.0 40.5 52-53 34.66714662712066 38.0 34.0 39.5 26.0 40.5 54-55 34.98784822213983 38.0 34.5 40.0 26.0 41.0 56-57 34.78724743933688 38.0 34.0 40.0 25.5 41.0 58-59 34.538270062917 38.0 34.0 40.0 25.0 41.0 60-61 34.23935719044603 38.0 34.0 40.0 23.5 41.0 62-63 33.90822312483669 37.5 33.5 40.0 23.0 41.0 64-65 33.53494123505567 37.0 33.0 40.0 22.0 41.0 66-67 33.14830436013861 36.0 32.5 39.0 21.5 41.0 68-69 32.665456733104136 36.0 32.0 39.0 20.0 40.0 70-71 32.22152249840279 35.0 31.5 38.5 18.5 40.0 72-73 31.83255308408223 35.0 31.0 38.0 18.0 40.0 74-75 31.37318133749588 35.0 31.0 37.0 14.5 39.0 76-77 30.925963762871717 35.0 31.0 36.5 10.0 39.0 78-79 30.444324473331754 34.5 30.0 36.0 7.0 38.5 80-81 29.953013695631654 34.0 29.5 35.5 4.5 37.5 82-83 29.469605506708273 34.0 29.0 35.0 2.0 37.0 84-85 29.023933495949862 34.0 29.0 35.0 2.0 36.5 86-87 28.58572800538468 34.0 29.0 35.0 2.0 36.0 88-89 28.161936377080647 34.0 27.5 35.0 2.0 36.0 90-91 27.717981621504784 33.0 26.5 35.0 2.0 35.0 92-93 27.24440703565023 33.0 26.0 35.0 2.0 35.0 94-95 26.690495962175923 33.0 25.0 35.0 2.0 35.0 96-97 26.021785986431595 32.0 24.5 35.0 2.0 35.0 98-99 25.21843308074417 32.0 21.0 34.0 2.0 35.0 100-101 23.242547160389787 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09659006477948395 1101 2 0.04685113484030623 1101 3 0.18859512708615966 1101 4 0.12589945369594346 1101 5 0.13245630106852246 1101 6 0.14121707134569306 1101 7 0.11252420677271147 1101 8 0.29709228374328234 1101 9 0.34610352899593266 1101 10-11 0.3568086911631383 1101 12-13 0.25473101894896644 1101 14-15 0.30056871696077536 1101 16-17 0.49646286238568393 1101 18-19 0.4363306717881059 1101 20-21 0.3905727563622676 1101 22-23 0.41898691844026104 1101 24-25 0.5764878701884371 1101 26-27 0.593894444576911 1101 28-29 0.36741203143374435 1101 30-31 0.26676319536178283 1101 32-33 0.18384327916951548 1101 34-35 0.20490258313652276 1101 36-37 0.3207577705788225 1101 38-39 0.3804560082359387 1101 40-41 0.35961034876660136 1101 42-43 0.4552687971490812 1101 44-45 0.44604367768423003 1101 46-47 0.2997109946140739 1101 48-49 0.31165491584986427 1101 50-51 0.3528410925951633 1101 52-53 0.3307893721143529 1101 54-55 0.5506370675898253 1101 56-57 0.3852051717332259 1101 58-59 0.5615981564129626 1101 60-61 0.5356318671409213 1101 62-63 0.5389011605577849 1101 64-65 0.6251348388777203 1101 66-67 0.5848559482653073 1101 68-69 0.7168938288719175 1101 70-71 0.7861446915085892 1101 72-73 0.7343741625332036 1101 74-75 0.5304033268065496 1101 76-77 0.5140535055826767 1101 78-79 0.6349238901982837 1101 80-81 0.5310298992990106 1101 82-83 0.6948313468892309 1101 84-85 0.739849169752798 1101 86-87 0.7706422985453365 1101 88-89 0.6877600556926673 1101 90-91 0.720778763533108 1101 92-93 1.0094972217581493 1101 94-95 1.1594714315117045 1101 96-97 1.246431714152589 1101 98-99 1.4010561833698745 1101 100-101 1.5331939796077485 1102 1 0.13178952121231902 1102 2 0.048298325735068204 1102 3 0.19056669185017938 1102 4 0.15803729559175395 1102 5 0.13023287487849444 1102 6 0.02191209550333184 1102 7 0.2935437353464323 1102 8 0.11094927828331436 1102 9 0.12774250710261015 1102 10-11 0.26607217060849564 1102 12-13 0.3346298046691487 1102 14-15 0.404209086379133 1102 16-17 0.41424893566359344 1102 18-19 0.42191788143885844 1102 20-21 0.3142198717795779 1102 22-23 0.37642962603967334 1102 24-25 0.4792250208015716 1102 26-27 0.41974563091711303 1102 28-29 0.4258732391052362 1102 30-31 0.3116985941207844 1102 32-33 0.4385600120004156 1102 34-35 0.3105120877874512 1102 36-37 0.5201471888356153 1102 38-39 0.48697921916948417 1102 40-41 0.6354625879037599 1102 42-43 0.4300034923157696 1102 44-45 0.33728003384058525 1102 46-47 0.48260085747512704 1102 48-49 0.3707002233718626 1102 50-51 0.21680919770641083 1102 52-53 0.5060920107474658 1102 54-55 0.35599576361170904 1102 56-57 0.33202779002306926 1102 58-59 0.4136753248102707 1102 60-61 0.4674292506975277 1102 62-63 0.3408234868179463 1102 64-65 0.5077218606447005 1102 66-67 0.5853928747136692 1102 68-69 0.5980686854890749 1102 70-71 0.652295730619187 1102 72-73 0.5747162891506683 1102 74-75 0.7220040721867989 1102 76-77 0.7568398218137276 1102 78-79 0.5640024256119425 1102 80-81 0.556858248043671 1102 82-83 0.7339190659317332 1102 84-85 0.8065375807258555 1102 86-87 0.7176722368514383 1102 88-89 0.7760154172636362 1102 90-91 0.8671825306025909 1102 92-93 0.7788012662801229 1102 94-95 0.5616896747013236 1102 96-97 0.8548157437086452 1102 98-99 1.083016750454906 1102 100-101 1.612345279488057 1103 1 0.2393877951894261 1103 2 0.18431937857033276 1103 3 0.2619126801047109 1103 4 0.11616866296179751 1103 5 0.17271828979156822 1103 6 0.31115879874156605 1103 7 0.16888598904471053 1103 8 0.2512636195598361 1103 9 0.22452895046990307 1103 10-11 0.39390332222567537 1103 12-13 0.4275486603082328 1103 14-15 0.44566290268927133 1103 16-17 0.32239312243010687 1103 18-19 0.2962176521409745 1103 20-21 0.2909611328211881 1103 22-23 0.39693340413349887 1103 24-25 0.5161073919545416 1103 26-27 0.5141520202978143 1103 28-29 0.5621567267550773 1103 30-31 0.49804908125785374 1103 32-33 0.33555616134396615 1103 34-35 0.4616370502967655 1103 36-37 0.47733645150263015 1103 38-39 0.26967109892837726 1103 40-41 0.2436268503289014 1103 42-43 0.25341872466128024 1103 44-45 0.3960847823385407 1103 46-47 0.43375411940684216 1103 48-49 0.5662395571502401 1103 50-51 0.46792666207816325 1103 52-53 0.4508972526712469 1103 54-55 0.2896529681125273 1103 56-57 0.30493653527020825 1103 58-59 0.25757861334827226 1103 60-61 0.19279182192983058 1103 62-63 0.1617486118675302 1103 64-65 0.31460732780285383 1103 66-67 0.3607801172373044 1103 68-69 0.30367971113754066 1103 70-71 0.4262752582848961 1103 72-73 0.3104988528364565 1103 74-75 0.3379917945912325 1103 76-77 0.6180589706058015 1103 78-79 0.6752155275188869 1103 80-81 0.43579235150829376 1103 82-83 0.44015533950249264 1103 84-85 0.5810020685788722 1103 86-87 0.7197027808776006 1103 88-89 0.7854546049492761 1103 90-91 0.9777667564561696 1103 92-93 1.0706009644196186 1103 94-95 0.9047246305348722 1103 96-97 1.0025520317919288 1103 98-99 1.2732324065661302 1103 100-101 1.4878713584942354 1104 1 0.1174083044646892 1104 2 0.14062310019724933 1104 3 0.199410087640139 1104 4 0.21992024724569603 1104 5 0.21965457277347866 1104 6 0.1174314107936425 1104 7 0.12007160950789597 1104 8 0.2651361855236587 1104 9 0.48500135512482245 1104 10-11 0.3280892883508031 1104 12-13 0.3874403528995458 1104 14-15 0.4850476244314095 1104 16-17 0.35920035828677044 1104 18-19 0.42730303064080033 1104 20-21 0.4120200105720855 1104 22-23 0.4683172041104555 1104 24-25 0.3362583310028171 1104 26-27 0.3898381592539195 1104 28-29 0.3057032182863608 1104 30-31 0.4112706505630612 1104 32-33 0.23278971359653156 1104 34-35 0.3766802580395918 1104 36-37 0.30122630056359156 1104 38-39 0.44130723212344947 1104 40-41 0.2856939087457633 1104 42-43 0.34154293251933865 1104 44-45 0.33474707062059395 1104 46-47 0.4743935940006878 1104 48-49 0.49996924951111765 1104 50-51 0.4116114183864923 1104 52-53 0.3986585154895437 1104 54-55 0.45299206247823776 1104 56-57 0.32975506275033695 1104 58-59 0.28630081636705995 1104 60-61 0.19505821206694662 1104 62-63 0.18807076879805606 1104 64-65 0.10890275354315548 1104 66-67 0.25405584024687045 1104 68-69 0.3504350977028068 1104 70-71 0.39345696124606633 1104 72-73 0.399166902150899 1104 74-75 0.3688962766744197 1104 76-77 0.41929413594742826 1104 78-79 0.45244216653259883 1104 80-81 0.31319412590626783 1104 82-83 0.486483257465391 1104 84-85 0.569788795160278 1104 86-87 0.5459477399742703 1104 88-89 0.453370263435275 1104 90-91 0.5284096876694413 1104 92-93 0.7733339981792682 1104 94-95 0.9256406346828143 1104 96-97 0.921580721733168 1104 98-99 1.0685221864946932 1104 100-101 1.1393404217503722 1105 1 -0.035079183848569784 1105 2 0.06896165819097888 1105 3 0.1027769687928668 1105 4 0.04465103974418838 1105 5 -0.08146295458724495 1105 6 0.028761774320010147 1105 7 0.13015355418149 1105 8 0.12251395596857861 1105 9 0.06805805643923435 1105 10-11 0.09195663697314416 1105 12-13 -0.08115943811404236 1105 14-15 0.043556121379104695 1105 16-17 0.09162075096124767 1105 18-19 0.1520800201364807 1105 20-21 0.170250775572363 1105 22-23 0.2134181225507561 1105 24-25 0.2228458028171545 1105 26-27 0.2082449128149335 1105 28-29 0.1347341812737639 1105 30-31 -0.11777846124466151 1105 32-33 -0.0366614220796464 1105 34-35 -0.021360219329380925 1105 36-37 -0.038834745468420806 1105 38-39 0.14861570707257954 1105 40-41 -0.07145012689191077 1105 42-43 0.11721746367481245 1105 44-45 -0.0812211414456172 1105 46-47 0.04032495846199424 1105 48-49 0.18411488004512222 1105 50-51 0.15710281144595228 1105 52-53 0.04416585751231139 1105 54-55 -0.0482998035504707 1105 56-57 0.07499512214647552 1105 58-59 0.01601758477710291 1105 60-61 0.11391978606463482 1105 62-63 -0.001331420059671018 1105 64-65 0.14691188294973756 1105 66-67 0.1872225344117453 1105 68-69 0.20144538203432916 1105 70-71 0.2524686754846428 1105 72-73 0.2567344102628688 1105 74-75 0.07462745414788685 1105 76-77 -0.04424818380178408 1105 78-79 0.059267222903933714 1105 80-81 0.3053054386709384 1105 82-83 0.29620057583970194 1105 84-85 0.36259746259689507 1105 86-87 0.18833568475785611 1105 88-89 0.30933847550149096 1105 90-91 0.18026526705678236 1105 92-93 0.3844933228231646 1105 94-95 0.38333133478731085 1105 96-97 0.7858357951533925 1105 98-99 0.9019303149663429 1105 100-101 1.1429454374243555 1106 1 0.19372751366111274 1106 2 0.18622129349244787 1106 3 0.10768723911827038 1106 4 0.13223138550674207 1106 5 0.041628619903292474 1106 6 0.13197464398073322 1106 7 0.10588897682801246 1106 8 0.10728831154872154 1106 9 0.16382329474089374 1106 10-11 -0.15577254315916633 1106 12-13 -0.2442556542087999 1106 14-15 -0.06785180356881426 1106 16-17 0.01731849752891179 1106 18-19 0.18229534844313378 1106 20-21 0.09902450882277236 1106 22-23 0.036638597642600246 1106 24-25 0.13988729011131085 1106 26-27 0.1545006053396989 1106 28-29 0.3508984201523049 1106 30-31 0.27081786368389515 1106 32-33 0.39573727102790457 1106 34-35 0.26488873252173306 1106 36-37 0.4319678640296516 1106 38-39 0.3184500099320786 1106 40-41 0.17801078551591587 1106 42-43 0.3069328734682202 1106 44-45 0.6632424705576625 1106 46-47 0.9073402258660792 1106 48-49 0.8138349683982113 1106 50-51 0.7886497923521745 1106 52-53 0.5901048048955104 1106 54-55 0.5549592604197855 1106 56-57 0.47667755585487015 1106 58-59 0.4493259362278508 1106 60-61 0.31531551941038316 1106 62-63 0.25884324616796306 1106 64-65 0.42517679511957596 1106 66-67 0.5749031033482552 1106 68-69 0.3697061219341222 1106 70-71 0.44185795345280354 1106 72-73 0.5005173029209651 1106 74-75 0.4509656571005536 1106 76-77 0.7355354124880371 1106 78-79 0.7453430754383952 1106 80-81 0.629528884611215 1106 82-83 0.5655882351592503 1106 84-85 0.34312101781442905 1106 86-87 0.7527743444429014 1106 88-89 0.8289758612583462 1106 90-91 1.0269385930951103 1106 92-93 1.075369688003594 1106 94-95 1.1059877219266667 1106 96-97 1.1664438305241909 1106 98-99 1.1742832794030065 1106 100-101 1.4804179656684795 1107 1 -0.016825090269300347 1107 2 -0.0077237773987341996 1107 3 0.07692950108327068 1107 4 0.10069895205300838 1107 5 0.0710801515649635 1107 6 0.04135166217515973 1107 7 0.025640287949038054 1107 8 0.05990629423894234 1107 9 0.193423358230973 1107 10-11 0.14450493468302028 1107 12-13 0.21080691482391245 1107 14-15 0.1427765224701929 1107 16-17 0.18137863690127887 1107 18-19 -0.016761692875654433 1107 20-21 -0.17042879211509643 1107 22-23 0.11367892621830578 1107 24-25 0.13220877706053358 1107 26-27 0.06735865623806347 1107 28-29 0.10543883427942546 1107 30-31 0.15908370362046043 1107 32-33 0.07351749197627555 1107 34-35 0.15480789917080529 1107 36-37 0.22099478396734895 1107 38-39 0.17403775547375488 1107 40-41 0.23353766213183036 1107 42-43 0.05346585663534853 1107 44-45 0.01935717644644086 1107 46-47 0.027247641026363567 1107 48-49 0.11180801860852085 1107 50-51 0.13823510459302213 1107 52-53 0.2062989243456883 1107 54-55 0.25686716631758344 1107 56-57 0.2105041507699852 1107 58-59 0.2956792250064879 1107 60-61 0.4429900934553217 1107 62-63 0.3750411881284208 1107 64-65 0.45747536884997686 1107 66-67 0.4518483060250347 1107 68-69 0.4687624715605736 1107 70-71 0.5093683998273519 1107 72-73 0.3982043565518829 1107 74-75 0.4159884137784786 1107 76-77 0.37616884248840776 1107 78-79 0.41783567251013665 1107 80-81 0.5840284524855299 1107 82-83 0.6437054194908924 1107 84-85 0.6479287164229639 1107 86-87 0.6018245052431688 1107 88-89 0.45379392797098106 1107 90-91 0.507548272564879 1107 92-93 0.5238103950442259 1107 94-95 0.71681194251682 1107 96-97 0.7989509676969782 1107 98-99 0.9180378436623151 1107 100-101 1.3869277370969257 1108 1 -0.10978163156025644 1108 2 -0.13883893589400031 1108 3 -0.34486674335460776 1108 4 -0.13579037763110335 1108 5 -0.24567919064426036 1108 6 -0.2755130545576847 1108 7 -0.07758586410463408 1108 8 -0.06187669908828042 1108 9 -0.013224188304747031 1108 10-11 -0.04061131914341587 1108 12-13 -0.1278195793621606 1108 14-15 -0.10024778979363447 1108 16-17 -0.18705880340704795 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-1.1826160663869416 2316 46-47 -0.8921340414167958 2316 48-49 -1.0343428217653994 2316 50-51 -1.0760279413864282 2316 52-53 -1.2916413609855297 2316 54-55 -1.407713080889522 2316 56-57 -1.486308434319234 2316 58-59 -1.3867965055073768 2316 60-61 -1.4581526476139928 2316 62-63 -2.0074586800705134 2316 64-65 -2.314502329376243 2316 66-67 -2.1088233418604148 2316 68-69 -2.0406721587296843 2316 70-71 -2.349053222990051 2316 72-73 -2.278592040265462 2316 74-75 -2.104534562506636 2316 76-77 -2.2703500071312313 2316 78-79 -2.199238560950384 2316 80-81 -2.170795466461467 2316 82-83 -2.245224014736241 2316 84-85 -2.6332541231818603 2316 86-87 -2.6782599921606085 2316 88-89 -2.649833237313757 2316 90-91 -2.754789082337652 2316 92-93 -2.588603766282347 2316 94-95 -2.796728678571146 2316 96-97 -2.8552268159156533 2316 98-99 -3.148432792611196 2316 100-101 -3.6779964333575137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5432.0 3 1294.0 4 1081.0 5 1260.0 6 1420.0 7 1748.0 8 1984.0 9 2527.0 10 3230.0 11 4175.0 12 5395.0 13 6484.0 14 7545.0 15 9232.0 16 11093.0 17 14024.0 18 12291.0 19 9213.0 20 9111.0 21 9704.0 22 10769.0 23 11985.0 24 13688.0 25 15550.0 26 17919.0 27 21297.0 28 25361.0 29 30650.0 30 37147.0 31 46071.0 32 58122.0 33 75230.0 34 101446.0 35 141592.0 36 204422.0 37 281450.0 38 223713.0 39 27271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64710379191248 19.272072482574853 14.677405740880149 22.40341798463252 2 14.991343642309971 20.323577668517746 40.23772745231458 24.4473512368577 3 19.35971072027743 24.84077132114192 29.601924873419545 26.19759308516111 4 12.520303454330946 16.3477789667188 37.81676132466321 33.31515625428705 5 13.594253518498956 37.8246011636186 33.90510513311923 14.67604018476321 6 31.50033688403855 36.43954353754972 17.580274702716114 14.479844875695616 7 28.658641055619594 32.86585039906467 20.47538244554066 18.000126099775077 8 26.53831497453162 34.6701035732497 19.74313669585433 19.048444756364354 9 26.4278826813307 16.94633459683615 20.43735792446533 36.188424797367816 10-11 24.83367012875913 27.00281317861765 27.19296321481059 20.970553477812633 12-13 25.98786241326807 24.17993840392345 27.845614338102624 21.986584844705853 14-15 22.750543833605423 25.58084307573371 26.447515773573322 25.221097317087548 16-17 22.000112398977993 29.115173313741188 26.72909379009354 22.155620497187282 18-19 21.832308310157803 27.654586150605077 29.22929999163899 21.283805547598128 20-21 22.744214700748806 26.433929823287023 29.312685410883194 21.509170065080976 22-23 22.32057715469315 26.504468974166123 29.263223178017135 21.911730693123587 24-25 22.218350707185326 26.631192625346294 29.345638584135557 21.80481808333282 26-27 22.034320477030374 26.889969726496922 29.2400377699296 21.835672026543108 28-29 21.7119680405401 27.136481875623886 29.343872260851477 21.807677822984534 30-31 21.906204589696486 26.789782853069394 29.350615523812134 21.953397033421986 32-33 21.717467851237547 26.907464681339583 29.253977365215082 22.12109010220779 34-35 22.264704864258704 26.814719207291265 29.11114667832845 21.809429250121582 36-37 22.144935223170066 26.694269329487604 29.132835759016697 22.027959688325645 38-39 21.955754704762974 26.983613589626394 29.202079874242713 21.85855183136792 40-41 22.150615441436962 26.904106528962373 29.125629814185544 21.819648215415125 42-43 21.889815095275754 26.980074532050292 28.850365738830813 22.279744633843144 44-45 22.074113213443507 27.10555910779599 28.650749431355983 22.169578247404523 46-47 22.23791684999911 27.26146564521369 28.29316468119345 22.207452823593755 48-49 22.24247602186588 27.362780507414225 28.103397874756126 22.291345595963772 50-51 22.101455732383226 27.479981794777242 28.22988480535628 22.18867766748325 52-53 22.45699949232715 27.632897337991167 27.811594068763107 22.098509100918577 54-55 22.117553521430846 27.43511800931463 27.977443377119183 22.46988509213534 56-57 22.262253812003948 27.700813277758098 27.814798242843136 22.22213466739482 58-59 22.579388572321303 27.334822982532597 27.828140802683837 22.257647642462267 60-61 22.403910488780266 27.33765479617626 28.390784396552288 21.867650318491194 62-63 22.246407598007302 27.481892375268103 28.237581629926062 22.03411839679853 64-65 22.10736057808593 27.512664130072075 28.103097290256446 22.276878001585544 66-67 22.08222577675702 27.645605648307292 27.821352994196914 22.450815580738777 68-69 21.999495044135678 27.57151514507889 28.110214667905876 22.318775142879556 70-71 22.183609379186343 27.51584771122116 27.906978337749656 22.393564571842845 72-73 22.188772047393954 27.350571422404446 28.037025197951603 22.42363133225 74-75 21.956808502619378 27.778135632128777 27.895862099683754 22.36919376556809 76-77 22.111471334170734 27.491655137016107 27.882875196757855 22.51399833205531 78-79 22.101975381566533 27.60799088338143 27.88315460768183 22.406879127370207 80-81 22.088577018354755 27.809318883433438 27.772211924251096 22.329892173960715 82-83 22.080503158444344 27.74052175486032 27.896234191504714 22.282740895190628 84-85 22.107777341558016 27.62360492466987 27.69054524501048 22.57807248876163 86-87 22.033547013231892 27.56888641620715 27.88944052225962 22.508126048301342 88-89 22.117447713523312 27.438268401115874 27.727411786429922 22.71687209893089 90-91 22.12278260833815 27.37877642223639 27.866339637405908 22.632101332019552 92-93 22.245541333410074 27.31652301774422 27.88601408328143 22.551921565564275 94-95 22.025311598785603 27.19558009686952 27.894112254357516 22.884996049987357 96-97 21.882154645691408 27.19716888860721 28.075416367619678 22.845260098081702 98-99 21.850456895958054 27.21473167833675 27.827124041145474 23.10768738455972 100-101 21.742833172724964 27.055961253930565 28.257320983902968 22.943884589441506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1797.0 1 1028.0 2 278.5 3 295.5 4 263.5 5 283.0 6 468.5 7 928.5 8 1533.5 9 1823.0 10 1707.5 11 1622.5 12 1695.0 13 1817.5 14 1951.5 15 2018.5 16 2046.5 17 2135.5 18 2283.5 19 2500.5 20 2724.0 21 2983.5 22 3460.5 23 4202.0 24 5134.5 25 6164.5 26 7630.5 27 9553.0 28 11501.0 29 13830.0 30 16647.0 31 19606.0 32 22514.0 33 25411.5 34 28826.0 35 31542.0 36 34813.5 37 38732.5 38 41433.5 39 43076.0 40 44367.0 41 46579.0 42 48706.0 43 51419.0 44 54591.0 45 56145.5 46 56363.5 47 58293.0 48 63584.5 49 63231.0 50 57484.0 51 53302.0 52 49458.0 53 47274.0 54 45043.0 55 41398.5 56 37782.0 57 34148.0 58 30861.0 59 27183.5 60 22786.0 61 18333.5 62 14310.0 63 10737.0 64 8011.5 65 6338.0 66 4767.5 67 3446.0 68 2422.5 69 1649.5 70 1187.5 71 879.0 72 677.5 73 521.0 74 381.5 75 279.0 76 194.5 77 125.0 78 73.5 79 44.5 80 28.5 81 19.5 82 13.0 83 11.5 84 12.0 85 14.0 86 12.5 87 6.5 88 2.5 89 3.0 90 5.5 91 5.0 92 5.0 93 4.0 94 4.5 95 4.5 96 4.5 97 7.5 98 9.0 99 8.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32101488037014186 2 0.2762109710067404 3 0.2708755436321674 4 0.27675819432720944 5 0.2777158351380303 6 0.20295144897894968 7 0.18906565722204818 8 0.19576914289779374 9 0.18845003098652052 10-11 0.2105441725504574 12-13 0.1986762667877854 14-15 0.19426427876650393 16-17 0.19412747293638666 18-19 0.1896128805425172 20-21 0.18243057446136127 22-23 0.18458526628570804 24-25 0.17435903048444312 26-27 0.17538507421032254 28-29 0.19340924232827106 30-31 0.2056191626662362 32-33 0.19758182014684736 34-35 0.20722663117011395 36-37 0.18102831470265937 38-39 0.17832639955784357 40-41 0.17559028295549844 42-43 0.18386703567759244 44-45 0.18831322515640325 46-47 0.1934434437858004 48-49 0.20113877172989605 50-51 0.20548235683611893 52-53 0.22637944738652982 54-55 0.2004889440368391 56-57 0.17258055469291878 58-59 0.17251215177786017 60-61 0.16871578999210632 62-63 0.17757396749219864 64-65 0.17025485558092546 66-67 0.16721092586081648 68-69 0.16365397427776782 70-71 0.17559028295549844 72-73 0.1738460086215034 74-75 0.1786000112180781 76-77 0.18092571033007143 78-79 0.17873681704819533 80-81 0.17996806951925062 82-83 0.17134930222186348 84-85 0.16532984569670423 86-87 0.16567186027199735 88-89 0.1670741200306992 90-91 0.17275156198056535 92-93 0.16861318561951835 94-95 0.1666979039978768 96-97 0.16611647921987843 98-99 0.17562448441302775 100-101 0.16221751306153664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1461926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.153362312662416 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5012886101756 47.822698180914344 2 11.954229408882307 14.381741856023634 3 3.6849004472220197 6.649774550635137 4 1.5814582761494316 3.8052013067030113 5 0.8507082831352951 2.55864817889102 6 0.5355519051161031 1.932914867141133 7 0.3364318532896386 1.4166255015116482 8 0.25602454673874736 1.2320589856728832 9 0.19309803104087864 1.0453946240757335 >10 1.0001944287875015 10.940427882394118 >50 0.07230650562502819 2.943694019285604 >100 0.03187264117879531 3.382050348060709 >500 9.106177217281225E-4 0.3639228185079991 >1k 9.106177217281225E-4 1.129564390766973 >5k 1.1382721521601532E-4 0.3952824894161202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5773 0.3948900286334603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.28106757797590304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.20575596849635341 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2510 0.17169131679715663 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1867 0.12770824241445874 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1592 0.108897440773336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.840291505862814E-5 0.0 0.0 6.840291505862814E-5 0.0 8 6.840291505862814E-5 0.0 0.0 6.840291505862814E-5 0.0 9 6.840291505862814E-5 0.0 0.0 6.840291505862814E-5 0.0 10-11 6.840291505862814E-5 0.0 0.0 6.840291505862814E-5 0.0 12-13 6.840291505862814E-5 0.0 0.0 6.840291505862814E-5 0.0 14-15 2.0520874517588442E-4 0.0 0.0 6.840291505862814E-5 6.840291505862814E-5 16-17 2.0520874517588442E-4 0.0 0.0 6.840291505862814E-5 6.840291505862814E-5 18-19 2.394102027051985E-4 0.0 0.0 6.840291505862814E-5 6.840291505862814E-5 20-21 2.7361166023451256E-4 0.0 0.0 6.840291505862814E-5 6.840291505862814E-5 22-23 2.7361166023451256E-4 0.0 0.0 1.3680583011725628E-4 1.0260437258794221E-4 24-25 2.7361166023451256E-4 0.0 0.0 3.762160328224548E-4 1.3680583011725628E-4 26-27 2.7361166023451256E-4 0.0 0.0 6.156262355276532E-4 1.3680583011725628E-4 28-29 3.420145752931407E-4 0.0 0.0 9.234393532914799E-4 1.3680583011725628E-4 30-31 3.420145752931407E-4 0.0 0.0 0.001881080164112274 1.3680583011725628E-4 32-33 3.420145752931407E-4 0.0 0.0 0.0030781311776382665 1.3680583011725628E-4 34-35 3.762160328224548E-4 0.0 0.0 0.0045145923938694574 1.3680583011725628E-4 36-37 5.472233204690251E-4 0.0 0.0 0.006429874015511045 1.3680583011725628E-4 38-39 6.498276930569672E-4 0.0 0.0 0.00882397604256303 1.3680583011725628E-4 40-41 6.840291505862814E-4 0.0 0.0 0.011902107220201297 1.3680583011725628E-4 42-43 6.840291505862814E-4 0.0 0.0 0.015664267548425842 1.3680583011725628E-4 44-45 6.840291505862814E-4 0.0 0.0 0.017511146255008804 1.3680583011725628E-4 46-47 6.840291505862814E-4 0.0 0.0 0.019871046824531474 1.3680583011725628E-4 48-49 6.840291505862814E-4 0.0 0.0 0.024830258166282017 1.3680583011725628E-4 50-51 6.840291505862814E-4 0.0 0.0 0.02968686513544461 1.3680583011725628E-4 52-53 6.840291505862814E-4 0.0 0.0 0.041828382558351106 2.0520874517588442E-4 54-55 6.840291505862814E-4 0.0 0.0 0.048429263861508726 2.7361166023451256E-4 56-57 6.840291505862814E-4 0.0 0.0 0.06847131797368676 2.7361166023451256E-4 58-59 6.840291505862814E-4 0.0 0.0 0.1093762611787464 2.7361166023451256E-4 60-61 6.840291505862814E-4 0.0 0.0 0.1471004688335798 2.7361166023451256E-4 62-63 7.182306081155955E-4 0.0 0.0 0.17004964683574955 2.7361166023451256E-4 64-65 7.524320656449096E-4 0.0 0.0 0.21068097838057467 2.7361166023451256E-4 66-67 7.524320656449096E-4 0.0 0.0 0.2953979886806856 2.7361166023451256E-4 68-69 7.524320656449096E-4 0.0 0.0 0.40812599269730476 2.7361166023451256E-4 70-71 7.524320656449096E-4 0.0 0.0 0.514766137273706 3.762160328224548E-4 72-73 7.866335231742237E-4 0.0 0.0 0.6341976269660707 4.1041749035176884E-4 74-75 8.208349807035377E-4 0.0 0.0 0.7822899380680006 4.1041749035176884E-4 76-77 8.208349807035377E-4 0.0 0.0 0.8825686115439495 4.1041749035176884E-4 78-79 8.208349807035377E-4 0.0 0.0 1.0146546405221606 4.1041749035176884E-4 80-81 9.91842268350108E-4 0.0 0.0 1.1917155861514195 4.446189478810829E-4 82-83 0.0011286480984673644 0.0 0.0 1.3524624365391955 4.7882040541039696E-4 84-85 0.0011628495559966783 0.0 0.0 1.5525409630856828 4.7882040541039696E-4 86-87 0.0011628495559966783 0.0 0.0 1.8360710460036964 4.7882040541039696E-4 88-89 0.0011628495559966783 0.0 0.0 2.2057203989805227 4.7882040541039696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8925 0.0 28.666481 1 GTACATG 7455 0.0 26.472855 1 TACATGG 7730 0.0 25.14727 2 TCAACGC 10490 0.0 24.155605 4 ATCAACG 10495 0.0 24.137474 3 TATCAAC 10605 0.0 23.88711 2 CAACGCA 10575 0.0 23.872358 5 ACATGGG 7780 0.0 23.702776 3 GGTATCA 6240 0.0 23.625307 1 AACGCAG 10780 0.0 23.488054 6 CATGGGG 3100 0.0 21.776741 4 GAGTACT 5140 0.0 21.16428 12-13 AGTACTT 5385 0.0 20.951208 12-13 ACGCAGA 12080 0.0 20.725124 7 TATAACG 115 0.0037111805 20.66423 2 CATGGGA 4590 0.0 20.61136 4 CGCAGAG 12130 0.0 20.561367 8 AGAGTAC 9595 0.0 20.002071 10-11 GTACTTT 5475 0.0 19.911997 14-15 GTGTAGC 765 0.0 19.892479 1 >>END_MODULE