FastQCFastQC Report
Thu 26 May 2016
SRR937246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461926
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79310.5425035193299798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60210.41185395156800003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.2832564712577791No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29800.20384068687471185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.10007346473077297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG702.0534218E-433.9290852
GTATCAA110200.025.3538841
GTATTCG950.001224162325.0089321
GTACATG67150.022.7855011
CCATGCG1050.002196312222.6193899
GGTATCA80500.022.0762081
AGTACTT65950.021.96773112-13
GCACCGT2606.2573235E-1021.9219076
TACATGG69300.021.7283232
GAGTACT65050.021.6874912-13
TCAACGC130000.021.44844
CAACGCA130100.021.4319135
ATCAACG130150.021.4236813
GTACTTT65750.021.41901214-15
AACGCAG132700.021.0105616
TATCAAC133400.020.8661332
ACATGGG70150.020.3816343
GTACCGT1404.8543492E-420.3560586
AGAGTAC110500.020.01048310-11
GGACCGT1707.958999E-519.5577836
Produced by FastQC (version 0.11.5)