##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937246_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1461926 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19920433729204 33.0 31.0 34.0 31.0 34.0 2 32.42972831730197 34.0 31.0 34.0 31.0 34.0 3 32.51571967390962 34.0 31.0 34.0 31.0 34.0 4 36.12144390345339 37.0 35.0 37.0 35.0 37.0 5 35.98363870674713 37.0 35.0 37.0 35.0 37.0 6 36.07294213250192 37.0 35.0 37.0 35.0 37.0 7 36.035417661359055 37.0 35.0 37.0 35.0 37.0 8 36.00935751878002 37.0 35.0 37.0 35.0 37.0 9 37.723794501226465 39.0 37.0 39.0 35.0 39.0 10-11 37.647652480358104 39.0 37.0 39.0 35.0 39.0 12-13 37.563532969521034 39.0 37.0 39.0 35.0 39.0 14-15 38.7935774450964 40.0 38.0 41.0 35.0 41.0 16-17 38.78025973954906 40.0 38.0 41.0 35.0 41.0 18-19 38.7920257249683 40.0 38.0 41.0 35.0 41.0 20-21 38.70839290087186 40.0 38.0 41.0 34.0 41.0 22-23 38.58260883245801 40.0 38.0 41.0 34.0 41.0 24-25 38.4418999320075 40.0 38.0 41.0 34.0 41.0 26-27 38.23655916920555 40.0 38.0 41.0 34.0 41.0 28-29 38.231119427385515 40.0 38.0 41.0 34.0 41.0 30-31 38.346055135485656 40.0 38.0 41.0 34.0 41.0 32-33 38.30041500048566 40.0 38.0 41.0 34.0 41.0 34-35 38.18697731622531 40.0 38.0 41.0 34.0 41.0 36-37 38.03834496410899 40.0 37.5 41.0 33.5 41.0 38-39 37.908080846773366 40.0 37.0 41.0 33.0 41.0 40-41 37.758361230322194 40.0 37.0 41.0 33.0 41.0 42-43 37.55639375727636 40.0 37.0 41.0 33.0 41.0 44-45 37.2763956588774 40.0 37.0 41.0 32.5 41.0 46-47 36.94568500731227 40.0 36.5 41.0 31.0 41.0 48-49 36.63103707027578 40.0 36.0 41.0 31.0 41.0 50-51 36.42876486224337 39.0 36.0 41.0 30.0 41.0 52-53 36.201714724274694 39.0 35.5 41.0 30.0 41.0 54-55 36.00281135980891 39.0 35.0 40.5 29.5 41.0 56-57 35.764499707919555 39.0 35.0 40.0 29.0 41.0 58-59 35.508359520249314 38.0 35.0 40.0 28.0 41.0 60-61 35.17184864350179 38.0 34.5 40.0 28.0 41.0 62-63 34.815464325827705 38.0 34.0 40.0 27.0 41.0 64-65 34.478149030799095 37.0 34.0 40.0 26.5 41.0 66-67 34.094738037356194 36.5 34.0 39.0 26.0 41.0 68-69 33.674261214315905 36.0 33.0 39.0 26.0 40.0 70-71 33.28605175638165 35.5 33.0 39.0 25.5 40.0 72-73 32.856184923176684 35.0 32.5 38.0 25.0 40.0 74-75 32.42181957226289 35.0 32.0 37.0 24.0 39.0 76-77 31.10324701797492 34.0 30.5 36.0 22.5 38.5 78-79 31.72377090222077 35.0 31.5 36.0 23.0 39.0 80-81 31.549430682537967 35.0 32.0 36.0 23.0 37.5 82-83 31.223227783075203 34.5 31.0 35.5 21.5 37.0 84-85 30.89414580491762 34.0 31.0 35.0 20.0 37.0 86-87 30.577150963865478 34.0 31.0 35.0 19.5 36.0 88-89 30.226625355866165 34.0 31.0 35.0 18.0 36.0 90-91 29.79554744905009 34.0 30.0 35.0 12.5 35.5 92-93 29.40449379790769 34.0 30.0 35.0 6.0 35.0 94-95 29.02670381400974 34.0 29.0 35.0 2.0 35.0 96-97 28.623310276990765 34.0 29.0 35.0 2.0 35.0 98-99 28.015714543690994 34.0 29.0 35.0 2.0 35.0 100-101 26.427659129121444 32.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16954501504775266 1101 2 0.20866107427480785 1101 3 0.21499198380769968 1101 4 0.14150086888879088 1101 5 0.0155630192781544 1101 6 0.026586045683700377 1101 7 0.08842444119684245 1101 8 0.1791900554244421 1101 9 0.1914780905198583 1101 10-11 0.22636544779570045 1101 12-13 0.23243208245418145 1101 14-15 0.29087440670576115 1101 16-17 0.3091432247042363 1101 18-19 0.31818200323260015 1101 20-21 0.2694184791690688 1101 22-23 0.23984187594303563 1101 24-25 0.18793698174811624 1101 26-27 0.13728731591154997 1101 28-29 0.16143787451043323 1101 30-31 0.22064916669634016 1101 32-33 0.22638805877049606 1101 34-35 0.34937516355500264 1101 36-37 0.34771016351205475 1101 38-39 0.2165834206649393 1101 40-41 0.20500797477831867 1101 42-43 0.05935267724071025 1101 44-45 0.03927048570454161 1101 46-47 -0.002161981053191653 1101 48-49 -0.0710075358753528 1101 50-51 -0.14051714201222865 1101 52-53 0.03177886348534287 1101 54-55 0.08860790572965271 1101 56-57 -0.028597878482003125 1101 58-59 -0.01060023427195489 1101 60-61 0.02808355913347782 1101 62-63 0.07460854747532863 1101 64-65 -0.014727475700986759 1101 66-67 0.030657682555727206 1101 68-69 -0.008708000266011595 1101 70-71 -0.2322695570164086 1101 72-73 -0.08036410099924751 1101 74-75 -0.05561027231791371 1101 76-77 -0.02752641558965152 1101 78-79 0.017650705767096042 1101 80-81 -0.18548872733660104 1101 82-83 0.03001378230450058 1101 84-85 -0.06848489543468261 1101 86-87 -0.11979965247588353 1101 88-89 -0.0729950906773098 1101 90-91 0.06503830862889615 1101 92-93 0.049623444212151924 1101 94-95 0.00792557205397415 1101 96-97 0.159870131458959 1101 98-99 0.11019186061559694 1101 100-101 0.4416040963362846 1102 1 0.16614043165570536 1102 2 0.1255706940855319 1102 3 -0.0049271775828358955 1102 4 0.09044548043101486 1102 5 -0.0855795893583462 1102 6 0.07168908211209413 1102 7 0.1074282361249459 1102 8 0.08938040111437573 1102 9 0.036327402881475734 1102 10-11 0.1068578085390115 1102 12-13 0.11679063756324126 1102 14-15 0.06245532710945412 1102 16-17 0.02665067121809983 1102 18-19 0.07861643584793399 1102 20-21 0.11358893995400621 1102 22-23 0.12355215107937312 1102 24-25 0.13728469304851387 1102 26-27 0.0918868643426407 1102 28-29 0.08453956011934594 1102 30-31 0.0027394302681145177 1102 32-33 0.12491078217806262 1102 34-35 0.11844050661294148 1102 36-37 0.16713442934814537 1102 38-39 0.022498884308618017 1102 40-41 0.08417482365984341 1102 42-43 0.2746243474463981 1102 44-45 0.07949360577360665 1102 46-47 0.08091584655697659 1102 48-49 0.18661775101182343 1102 50-51 0.11792406251706922 1102 52-53 0.043099062771709384 1102 54-55 0.10701444084528333 1102 56-57 0.08736568178576931 1102 58-59 -0.10154812406193514 1102 60-61 -0.16763503957756853 1102 62-63 -0.10119153772023282 1102 64-65 0.0317569518123193 1102 66-67 0.03664283325956319 1102 68-69 -0.0037096442489570336 1102 70-71 -0.09998501191377329 1102 72-73 -0.1180615206748925 1102 74-75 -0.17796005843815976 1102 76-77 -0.12901968659740604 1102 78-79 0.06966029269816332 1102 80-81 0.042342000729181706 1102 82-83 0.019686249072560003 1102 84-85 0.03182883805867576 1102 86-87 0.1264272851969146 1102 88-89 0.12507650923393143 1102 90-91 -0.10534493375897469 1102 92-93 -0.04861850634526377 1102 94-95 0.01554721464398412 1102 96-97 0.10434987159627696 1102 98-99 0.011187442107750911 1102 100-101 0.15703328116369875 1103 1 0.14643726115289013 1103 2 0.155855913563272 1103 3 0.1471906834645793 1103 4 0.21893211439612514 1103 5 0.04536449872300352 1103 6 0.14655677626548425 1103 7 0.21524177921263288 1103 8 0.1314933279035273 1103 9 0.21665035492242168 1103 10-11 0.2475555223326822 1103 12-13 0.25238236155437477 1103 14-15 0.22115098979968906 1103 16-17 0.15163494931676524 1103 18-19 0.2366360567807959 1103 20-21 0.24644747186705018 1103 22-23 0.2679457210636045 1103 24-25 0.3017660154297346 1103 26-27 0.2715797309531922 1103 28-29 0.1676552055880478 1103 30-31 0.1912583304122748 1103 32-33 0.21488238600549892 1103 34-35 0.2693973657156832 1103 36-37 0.2763822935883198 1103 38-39 0.39547680592164625 1103 40-41 0.4007316788925763 1103 42-43 0.3884714725153344 1103 44-45 0.373627401572719 1103 46-47 0.4488446190876729 1103 48-49 0.26511962312745396 1103 50-51 0.3141007379879994 1103 52-53 0.43391287675984813 1103 54-55 0.24484714046506184 1103 56-57 0.4026841349441739 1103 58-59 0.3152612490937656 1103 60-61 0.15914347027643316 1103 62-63 0.35407360609887917 1103 64-65 0.49161893770614995 1103 66-67 0.3819635116716995 1103 68-69 0.34046938225091594 1103 70-71 0.23931417828735846 1103 72-73 0.3399005339115533 1103 74-75 0.2946197362579923 1103 76-77 0.0749248175096433 1103 78-79 0.5042245299682584 1103 80-81 0.4897643345365914 1103 82-83 0.3943077140635154 1103 84-85 0.4412440800792048 1103 86-87 0.5649159509154309 1103 88-89 0.2825688417835899 1103 90-91 0.43962816969597185 1103 92-93 0.3537714645329366 1103 94-95 0.2163340353369101 1103 96-97 0.5125666209843338 1103 98-99 0.42011288643286804 1103 100-101 0.6986273852088232 1104 1 0.10238840759833323 1104 2 0.14452281067123351 1104 3 0.08449452031214832 1104 4 0.05455142537665125 1104 5 -0.1078904845699995 1104 6 0.01280173536215301 1104 7 0.04178400757995604 1104 8 -0.01370773212643428 1104 9 0.03156681995294974 1104 10-11 0.043689869607717924 1104 12-13 -0.003798073979666583 1104 14-15 0.10486916009580938 1104 16-17 0.04903096268236595 1104 18-19 0.09929189270658867 1104 20-21 -0.01552625782531436 1104 22-23 0.05269742056815119 1104 24-25 -0.028976005540428673 1104 26-27 -0.05828315647623583 1104 28-29 0.003954318389062905 1104 30-31 0.053816228787660236 1104 32-33 -0.12679983544857976 1104 34-35 -0.03566312512428027 1104 36-37 0.062049997240812615 1104 38-39 0.03993469277496331 1104 40-41 0.1489307801715185 1104 42-43 0.1430849072428373 1104 44-45 0.1345606888177855 1104 46-47 0.12284968879616542 1104 48-49 -0.06890503409111659 1104 50-51 -0.08367089468858069 1104 52-53 0.08804123256120988 1104 54-55 0.0648932287240811 1104 56-57 -0.05024477391629034 1104 58-59 -0.05553146896131267 1104 60-61 -0.12731119039875693 1104 62-63 -0.13888295571700127 1104 64-65 -0.06423772780831172 1104 66-67 -0.31977632010615054 1104 68-69 -0.25220143235906534 1104 70-71 -0.06490404961907359 1104 72-73 -0.05252601292563952 1104 74-75 -0.2318982957733482 1104 76-77 -0.16040298523261143 1104 78-79 -0.20994530526621702 1104 80-81 -0.16315996549797518 1104 82-83 -0.09608057951869498 1104 84-85 -0.030447359026684495 1104 86-87 0.010461520681534608 1104 88-89 0.0737417879755462 1104 90-91 0.06349036580151335 1104 92-93 0.12509534977317216 1104 94-95 -0.02509470069258768 1104 96-97 0.05535496065479961 1104 98-99 0.09100354203835437 1104 100-101 0.26810082684956527 1105 1 -0.06691258783695986 1105 2 0.014055891021008904 1105 3 -0.08292669730870728 1105 4 -0.012911589527377032 1105 5 -0.09193052196756923 1105 6 0.039267115112465945 1105 7 0.034380388815179685 1105 8 0.02866151387257787 1105 9 -0.060514794742090317 1105 10-11 0.04033233784727486 1105 12-13 3.420569118262051E-4 1105 14-15 0.037315401090147304 1105 16-17 0.020625309049854934 1105 18-19 0.017238326188412145 1105 20-21 0.15263026200089058 1105 22-23 -0.01194922739972526 1105 24-25 -0.038791767515903075 1105 26-27 0.058158574811230324 1105 28-29 -0.03396461688014796 1105 30-31 -0.061829154262447616 1105 32-33 0.03441778540606322 1105 34-35 -0.10489042534975823 1105 36-37 -0.12411950174175246 1105 38-39 -0.015584198497101909 1105 40-41 0.05081770752124015 1105 42-43 -0.17544503831070557 1105 44-45 -0.01945844993819179 1105 46-47 0.13913523023596497 1105 48-49 0.10438870181604898 1105 50-51 0.2769692637011332 1105 52-53 0.22943099222682406 1105 54-55 0.1991197040031878 1105 56-57 0.21318480316119803 1105 58-59 0.17751423306805947 1105 60-61 0.057780928613510696 1105 62-63 0.22685647122025188 1105 64-65 0.20126081478642277 1105 66-67 0.13199456478632499 1105 68-69 0.16631201426690723 1105 70-71 0.01576855666151289 1105 72-73 0.07066542963059419 1105 74-75 0.1934055594760622 1105 76-77 0.08143389537015011 1105 78-79 -0.0030729519691803375 1105 80-81 -0.08972818856347331 1105 82-83 -0.12229132582018565 1105 84-85 -0.0392401874928332 1105 86-87 -0.023527920004820402 1105 88-89 0.007296206938313787 1105 90-91 -0.13404304452681615 1105 92-93 0.06457783341173595 1105 94-95 0.09199103873435277 1105 96-97 -0.05415502119637594 1105 98-99 0.043304042505820206 1105 100-101 0.07994870673737253 1106 1 -0.03890479319395013 1106 2 0.04751683151497588 1106 3 -0.04405067507483551 1106 4 0.010102785033083705 1106 5 -0.0734705367719215 1106 6 0.02874257318448059 1106 7 -0.03211206987140258 1106 8 -0.030297576816458616 1106 9 -0.1006380633331787 1106 10-11 -0.12070638370118303 1106 12-13 -0.23643241988218477 1106 14-15 -0.08263588423880464 1106 16-17 0.11447773338264966 1106 18-19 0.02868490653455069 1106 20-21 -0.2129433733914894 1106 22-23 -0.06119143746852984 1106 24-25 0.04085313497900245 1106 26-27 0.07749033901625779 1106 28-29 0.09891257536320097 1106 30-31 -0.0035847819303924666 1106 32-33 0.02390155845378672 1106 34-35 0.09834735228265856 1106 36-37 0.03335856242842539 1106 38-39 0.00695677677933304 1106 40-41 0.03537993883204393 1106 42-43 -0.10047241305853305 1106 44-45 0.004590677155661638 1106 46-47 0.09465583825655699 1106 48-49 -0.06695208986572254 1106 50-51 0.11928240125189404 1106 52-53 0.23397392540287854 1106 54-55 0.06705312950339959 1106 56-57 0.1488311410056795 1106 58-59 0.003434303608621292 1106 60-61 0.17640606770456912 1106 62-63 0.2907903194907888 1106 64-65 0.1644736992293261 1106 66-67 0.26582650101896377 1106 68-69 0.2912855449275469 1106 70-71 0.3415154909299716 1106 72-73 0.4638126331564436 1106 74-75 0.2681313223381281 1106 76-77 0.34838692980043007 1106 78-79 0.308181349182977 1106 80-81 0.28604071571858825 1106 82-83 0.23434962355048228 1106 84-85 0.263983325336806 1106 86-87 0.41870812848220496 1106 88-89 0.39727041573141975 1106 90-91 0.37713228283378086 1106 92-93 0.41016171285419745 1106 94-95 0.12079097339711709 1106 96-97 0.4131761783294081 1106 98-99 0.48074382822335693 1106 100-101 0.6066674953673648 1107 1 -0.10200715710712416 1107 2 -0.029797989704391625 1107 3 -0.0318987328391529 1107 4 -0.00553164610093404 1107 5 -0.07537341879906023 1107 6 -0.04104736349290761 1107 7 -6.523574723615866E-5 1107 8 -0.07905970236517135 1107 9 -0.05582004030637222 1107 10-11 -0.05531688446459526 1107 12-13 0.10737034441163473 1107 14-15 0.023888194299580334 1107 16-17 -0.10109364453401781 1107 18-19 0.026235223511662298 1107 20-21 -0.10015947919274026 1107 22-23 -0.06313600306026501 1107 24-25 0.005030206761375666 1107 26-27 0.03952953540081694 1107 28-29 -0.05912474398677858 1107 30-31 0.07089391867821604 1107 32-33 -0.06883472546917346 1107 34-35 -0.1402750368518042 1107 36-37 -0.00870270258220529 1107 38-39 0.060269145767350096 1107 40-41 0.056545906404046775 1107 42-43 0.0921631750219376 1107 44-45 0.011373994701415313 1107 46-47 0.05118898160505836 1107 48-49 0.026491722509454974 1107 50-51 0.1549800967514514 1107 52-53 0.10454572925640093 1107 54-55 0.13691114326881149 1107 56-57 0.12249550130312059 1107 58-59 0.18350056773461176 1107 60-61 0.010413012295643398 1107 62-63 -0.0024340807937548448 1107 64-65 -0.001994075552737229 1107 66-67 0.1425327057904795 1107 68-69 0.005143892161470376 1107 70-71 6.205648209132164E-4 1107 72-73 0.11876773504271654 1107 74-75 0.12572260934457802 1107 76-77 0.20514691183099387 1107 78-79 0.12237094423716144 1107 80-81 0.15571495775120425 1107 82-83 0.12469054050152906 1107 84-85 0.16344074730480784 1107 86-87 0.19447676249177093 1107 88-89 0.27440383153389547 1107 90-91 0.24790904604856223 1107 92-93 0.2101186459049309 1107 94-95 0.1971334208084805 1107 96-97 0.24405976796509066 1107 98-99 0.19723643111987244 1107 100-101 0.5411574960444945 1108 1 -0.06123003585452125 1108 2 -0.1143249215295441 1108 3 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-1.3426097690358603 2316 98-99 -1.13013131038878 2316 100-101 -1.2645152734421643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 4.0 5 36.0 6 53.0 7 136.0 8 287.0 9 621.0 10 1009.0 11 1651.0 12 2597.0 13 3687.0 14 5018.0 15 7078.0 16 9522.0 17 13758.0 18 12296.0 19 7090.0 20 5988.0 21 6071.0 22 6295.0 23 6807.0 24 7827.0 25 9375.0 26 11646.0 27 14683.0 28 18696.0 29 23429.0 30 29768.0 31 38369.0 32 49555.0 33 65809.0 34 92086.0 35 133565.0 36 208775.0 37 336697.0 38 299466.0 39 32163.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.938218090912386 19.893611852293887 15.359078817973343 21.809091238820383 2 15.772754571708827 20.995180330604967 39.50726644166668 23.724798656019523 3 19.757429582619093 24.79455184462141 29.970326815447567 25.47769175731193 4 13.092181136391309 16.915835685253562 37.57098512510209 32.42099805325304 5 13.650804509632225 37.80511164623467 33.96104695709282 14.58303688704028 6 31.718910533091275 36.71779556557582 17.14026564956092 14.423028251771978 7 28.5736760957805 33.10318032513273 20.3504828561774 17.972660722909367 8 26.811617003870236 34.7332901938949 19.43545706143813 19.01963574079673 9 26.550454674176393 16.864328290214416 20.259233367489188 36.325983668119996 10-11 25.02571949606204 27.15694912054372 26.932382350406247 20.88494903298799 12-13 26.282072330167487 24.213737095136853 27.881276627182505 21.622913947513155 14-15 22.890590905422027 25.498486243489754 26.620259848993726 24.9906630020945 16-17 21.874294594938455 29.147097732716976 26.96754144874638 22.011066223598185 18-19 21.779624960497316 27.3644835648316 29.823841972849515 21.03204950182157 20-21 22.569986442542238 26.084938635744905 30.154091246752575 21.190983674960293 22-23 22.23135410820293 26.17954382296317 30.020100409703605 21.569001659130297 24-25 22.205672415055027 26.336036861189793 30.134474118571713 21.323816605183467 26-27 22.017579861823656 26.558998563513235 30.06204254736986 21.361379027293246 28-29 21.628706117181828 26.790276015884913 30.188375941973767 21.392641924959495 30-31 21.846375263097052 26.454241805086898 30.09645580824577 21.602927123570282 32-33 21.74053269454131 26.571693779302098 29.95479935372926 21.732974172427333 34-35 22.34740335694146 26.406911156925865 29.843199997811105 21.40248548832157 36-37 22.20401560274228 26.28446446374551 29.882261200576636 21.62925873293557 38-39 21.955351227819005 26.663709371536022 29.94661478061579 21.434324620029187 40-41 22.14979491088978 26.538322233246497 29.824707209772317 21.487175646091405 42-43 21.945646567234427 26.69548340842515 29.505235448081297 21.85363457625913 44-45 22.116885533193884 26.654016687575155 29.2941297986355 21.93496798059546 46-47 22.26897941482674 26.990114410715726 28.911962712202943 21.82894346225459 48-49 22.311252416332973 27.110913958709265 28.644062695375826 21.933770929581936 50-51 22.22123418011582 27.23547566703103 28.65298927579098 21.890300877062177 52-53 22.479691146735398 27.447528052073842 28.209161351752893 21.863619449437863 54-55 22.20926366998056 27.231405693585035 28.3223637858551 22.236966850579304 56-57 22.33341496081197 27.447422099340184 28.255260526182585 21.96390241366526 58-59 22.643758986432967 27.12305547613217 28.211345854714942 22.021839682719918 60-61 22.450828564510104 27.131879452174733 28.79444650413222 21.622845479182942 62-63 22.332252111255972 27.265025726336354 28.628193219082227 21.77452894332545 64-65 22.192504955791197 27.28564920522653 28.49302905892638 22.028816780055898 66-67 22.126803955877385 27.422455035343784 28.279338352283222 22.17140265649561 68-69 22.169931993821848 27.30110826402978 28.44254770761311 22.086412034535265 70-71 22.248868957799505 27.310000642987404 28.267675655265723 22.173454743947367 72-73 22.315185583948846 27.16515747035076 28.365662831087207 22.153994114613187 74-75 22.079708548859518 27.658410890838525 28.074198010022393 22.187682550279565 76-77 22.218019243108063 27.237425150110194 28.28074061204192 22.263814994739818 78-79 22.14383628172698 27.34403109322907 28.274242335111353 22.237890289932597 80-81 22.231973437780024 27.589768565577188 28.13073301931835 22.047524977324436 82-83 22.179363878510618 27.445068888764247 28.308213434095663 22.067353798629476 84-85 22.129760418626127 27.426885784154454 28.020828701882795 22.422525095336628 86-87 22.121707938705516 27.376112060391566 28.097831217175152 22.40434878372777 88-89 22.150197752827435 27.290334804908046 28.016260740967734 22.543206701296782 90-91 22.13380480169871 27.203765727013522 28.132541988868642 22.529887482419127 92-93 22.246414582726246 27.07833126067964 28.12132753811152 22.5539266184826 94-95 22.072115825288012 26.9480808194122 28.186960215496544 22.792843139803246 96-97 21.86266609937849 27.07041943299456 28.306494309561497 22.760420158065457 98-99 21.802002452960416 27.08359697299486 27.959576087945404 23.154824486099322 100-101 21.42494322782037 27.307700595889827 28.240375409565228 23.026980766724574 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 211.0 2 195.5 3 293.0 4 265.0 5 237.0 6 500.0 7 1113.0 8 1808.5 9 2257.0 10 2167.0 11 1878.0 12 1918.5 13 2105.5 14 2262.0 15 2331.0 16 2381.0 17 2522.5 18 2676.5 19 2833.0 20 3019.5 21 3305.5 22 3802.5 23 4437.0 24 5270.5 25 6381.0 26 7688.0 27 9531.0 28 11469.0 29 13779.5 30 16720.5 31 19395.0 32 22126.0 33 25189.5 34 28670.5 35 31470.0 36 35027.0 37 38960.0 38 41422.0 39 42978.0 40 44197.5 41 46432.0 42 49065.0 43 51841.5 44 54548.5 45 56211.0 46 56306.5 47 58545.0 48 64624.0 49 63359.0 50 57023.0 51 53510.0 52 49563.0 53 47137.5 54 45298.5 55 41617.0 56 37636.0 57 33660.5 58 29977.5 59 26446.5 60 22546.0 61 18122.5 62 13843.0 63 10471.0 64 7823.0 65 6055.0 66 4482.5 67 3336.0 68 2320.5 69 1429.5 70 982.5 71 722.0 72 573.0 73 454.5 74 351.0 75 258.0 76 183.5 77 93.5 78 49.5 79 32.0 80 15.5 81 11.5 82 7.5 83 4.5 84 4.5 85 2.0 86 2.0 87 1.5 88 1.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04603516183445674 2 0.0 3 0.0 4 0.0 5 0.011354883899732271 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005061815714338482 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010602451834087361 24-25 6.156262355276532E-4 26-27 0.0017784757915243316 28-29 0.002428303484581299 30-31 8.892378957621658E-4 32-33 0.0 34-35 0.0 36-37 2.3941020270519848E-4 38-39 3.078131177638266E-4 40-41 3.420145752931407E-5 42-43 0.010944466409380502 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.3680583011725628E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7361166023451256E-4 84-85 6.840291505862814E-5 86-87 0.0 88-89 0.0 90-91 0.007524320656449095 92-93 0.0021888932818761005 94-95 0.0 96-97 0.0 98-99 0.0012996553861139345 100-101 0.011115473697027072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1461926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.04540118931144 #Duplication Level Percentage of deduplicated Percentage of total 1 78.11986173107744 45.34498715033928 2 12.661341459431757 14.698652892151568 3 3.9767277806384755 6.924922783435212 4 1.6843098062293178 3.9106575371868875 5 0.9353809511531145 2.7147281287261125 6 0.5839169003586927 2.0336214445523706 7 0.37836575480702606 1.5373674423849328 8 0.26719123698776026 1.2407378036178347 9 0.1965971326680784 1.0270403494548208 >10 1.0836417072754874 11.500158125595613 >50 0.0761588733374282 3.0805915681589173 >100 0.03378939610473086 3.539945359182507 >500 0.0017721325634655293 0.6592220211467246 >1k 7.088530253862118E-4 0.8305936877279858 >5k 2.362843417954039E-4 0.9567737063392386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7931 0.5425035193299798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6021 0.41185395156800003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4141 0.2832564712577791 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2980 0.20384068687471185 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1463 0.10007346473077297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 6.840291505862814E-5 9 0.0 0.0 0.0 0.0 6.840291505862814E-5 10-11 0.0 0.0 0.0 0.0 6.840291505862814E-5 12-13 0.0 0.0 0.0 0.0 6.840291505862814E-5 14-15 0.0 0.0 0.0 0.0 6.840291505862814E-5 16-17 0.0 0.0 0.0 0.0 6.840291505862814E-5 18-19 3.420145752931407E-5 0.0 0.0 0.0 6.840291505862814E-5 20-21 6.840291505862814E-5 0.0 0.0 0.0 6.840291505862814E-5 22-23 6.840291505862814E-5 0.0 0.0 6.840291505862814E-5 1.0260437258794221E-4 24-25 6.840291505862814E-5 0.0 0.0 3.078131177638266E-4 1.3680583011725628E-4 26-27 6.840291505862814E-5 0.0 0.0 5.472233204690251E-4 1.3680583011725628E-4 28-29 1.0260437258794221E-4 0.0 0.0 9.234393532914799E-4 1.3680583011725628E-4 30-31 1.3680583011725628E-4 0.0 0.0 0.0018468787065829598 1.3680583011725628E-4 32-33 1.3680583011725628E-4 0.0 0.0 0.0030097282625796383 1.3680583011725628E-4 34-35 1.3680583011725628E-4 0.0 0.0 0.004446189478810829 1.3680583011725628E-4 36-37 1.3680583011725628E-4 0.0 0.0 0.006327269642923103 1.3680583011725628E-4 38-39 1.3680583011725628E-4 0.0 0.0 0.00882397604256303 1.3680583011725628E-4 40-41 1.7100728764657034E-4 0.0 0.0 0.01193630867773061 1.3680583011725628E-4 42-43 2.0520874517588442E-4 0.0 0.0 0.015766871921013784 1.3680583011725628E-4 44-45 2.7361166023451256E-4 0.0 0.0 0.017545347712538118 1.3680583011725628E-4 46-47 3.078131177638266E-4 0.0 0.0 0.019802643909472846 1.3680583011725628E-4 48-49 3.420145752931407E-4 0.0 0.0 0.024590847963576815 1.3680583011725628E-4 50-51 3.420145752931407E-4 0.0 0.0 0.029379052017680787 1.3680583011725628E-4 52-53 3.420145752931407E-4 0.0 0.0 0.04152056944058728 1.3680583011725628E-4 54-55 3.420145752931407E-4 0.0 0.0 0.04805304782868627 1.3680583011725628E-4 56-57 4.1041749035176884E-4 0.0 0.0 0.06809510194086431 1.3680583011725628E-4 58-59 4.1041749035176884E-4 0.0 0.0 0.10896584368839463 1.3680583011725628E-4 60-61 4.1041749035176884E-4 0.0 0.0 0.14669005134322805 1.3680583011725628E-4 62-63 4.1041749035176884E-4 0.0 0.0 0.17001544537822022 1.3680583011725628E-4 64-65 4.1041749035176884E-4 0.0 0.0 0.2112282017010437 1.3680583011725628E-4 66-67 4.1041749035176884E-4 0.0 0.0 0.29594521200115464 1.3680583011725628E-4 68-69 4.1041749035176884E-4 0.0 0.0 0.40959665537106527 1.3680583011725628E-4 70-71 4.1041749035176884E-4 0.0 0.0 0.5168866276405235 1.3680583011725628E-4 72-73 4.1041749035176884E-4 0.0 0.0 0.637822981464178 2.0520874517588442E-4 74-75 4.1041749035176884E-4 0.0 0.0 0.7868729333769288 2.0520874517588442E-4 76-77 4.1041749035176884E-4 0.0 0.0 0.8874936214281708 2.0520874517588442E-4 78-79 4.1041749035176884E-4 0.0 0.0 1.0208451043349664 2.0520874517588442E-4 80-81 4.7882040541039696E-4 0.0 0.0 1.200710569481629 2.0520874517588442E-4 82-83 4.7882040541039696E-4 0.0 0.0 1.3636463131512813 2.0520874517588442E-4 84-85 5.13021862939711E-4 0.0 0.0 1.5663583519275257 2.0520874517588442E-4 86-87 5.472233204690251E-4 0.0 0.0 1.8535821922587052 2.7361166023451256E-4 88-89 5.472233204690251E-4 0.0 0.0 2.2293536061332793 2.7361166023451256E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 70 2.0534218E-4 33.929085 2 GTATCAA 11020 0.0 25.353884 1 GTATTCG 95 0.0012241623 25.008932 1 GTACATG 6715 0.0 22.785501 1 CCATGCG 105 0.0021963122 22.619389 9 GGTATCA 8050 0.0 22.076208 1 AGTACTT 6595 0.0 21.967731 12-13 GCACCGT 260 6.2573235E-10 21.921907 6 TACATGG 6930 0.0 21.728323 2 GAGTACT 6505 0.0 21.68749 12-13 TCAACGC 13000 0.0 21.4484 4 CAACGCA 13010 0.0 21.431913 5 ATCAACG 13015 0.0 21.423681 3 GTACTTT 6575 0.0 21.419012 14-15 AACGCAG 13270 0.0 21.010561 6 TATCAAC 13340 0.0 20.866133 2 ACATGGG 7015 0.0 20.381634 3 GTACCGT 140 4.8543492E-4 20.356058 6 AGAGTAC 11050 0.0 20.010483 10-11 GGACCGT 170 7.958999E-5 19.557783 6 >>END_MODULE