FastQCFastQC Report
Thu 26 May 2016
SRR937245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529522
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59620.38979498170016513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42160.27564167105801685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.19496287075308497No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27610.18051391218955987No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22710.1484777597183957No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16550.10820373946893212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101300.025.8636131
GTACATG71150.024.1924951
GGTATCA73750.023.0172391
TACATGG73750.022.6859342
GAGTACT52650.022.5988512-13
ATCAACG117500.022.1271513
TCAACGC118250.021.9466154
TATCAAC119050.021.9189132
CAACGCA119000.021.8068665
ACATGGG74300.021.7503453
AACGCAG120600.021.5850456
AGTACTT54950.021.3071912-13
GTACTTT55550.020.86397214-15
ATTCGCG1850.020.53638880-81
AGAGTAC96900.019.7544710-11
ACGCAGA133950.019.3628657
CGCAGAG134600.019.2334378
CATGGGG32600.018.5166384
TACTTTT62400.018.45943314-15
CATGGGA43050.018.4382344
Produced by FastQC (version 0.11.5)