##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937245_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1529522 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52227754814903 31.0 31.0 34.0 30.0 34.0 2 31.61088823828621 33.0 31.0 34.0 30.0 34.0 3 31.65963941675896 33.0 31.0 34.0 30.0 34.0 4 35.30336863412229 37.0 35.0 37.0 33.0 37.0 5 35.281166926660745 37.0 35.0 37.0 33.0 37.0 6 35.347976688141785 37.0 35.0 37.0 33.0 37.0 7 35.283275428532576 37.0 35.0 37.0 33.0 37.0 8 35.27303497432531 37.0 35.0 37.0 33.0 37.0 9 36.85881602226055 39.0 37.0 39.0 33.0 39.0 10-11 36.756024104262636 39.0 37.0 39.0 33.0 39.0 12-13 36.63531776594256 39.0 37.0 39.0 32.0 39.0 14-15 37.7547776364119 40.0 37.5 41.0 32.5 41.0 16-17 37.698954967630414 40.0 37.0 41.0 32.0 41.0 18-19 37.645030931232114 40.0 37.0 41.0 32.0 41.0 20-21 37.572248388712296 40.0 37.0 41.0 32.0 41.0 22-23 37.43760632406726 40.0 37.0 41.0 32.0 41.0 24-25 37.28554705326239 39.5 37.0 41.0 31.5 41.0 26-27 37.11414448435524 39.0 36.5 41.0 31.0 41.0 28-29 36.93422487548398 39.0 36.0 40.5 31.0 41.0 30-31 36.94387168017198 39.0 36.0 41.0 31.0 41.0 32-33 36.80846695895842 39.0 36.0 41.0 30.0 41.0 34-35 36.60539338433837 39.0 35.5 40.5 30.0 41.0 36-37 36.61397645800453 39.0 36.0 41.0 30.0 41.0 38-39 36.59265476403739 39.0 36.0 41.0 30.0 41.0 40-41 36.44699683953549 39.0 35.5 41.0 30.0 41.0 42-43 36.220127268519185 39.0 35.0 41.0 30.0 41.0 44-45 36.00227685512206 39.0 35.0 41.0 29.0 41.0 46-47 35.69623222156987 39.0 35.0 40.5 27.5 41.0 48-49 35.40826643879591 39.0 35.0 40.0 26.5 41.0 50-51 34.47789701619199 38.0 34.0 39.5 25.0 40.5 52-53 34.620530793280516 38.0 34.0 39.5 26.0 40.5 54-55 34.972448581975286 38.0 34.0 40.0 26.0 41.0 56-57 34.75338896727213 38.0 34.0 40.0 25.5 41.0 58-59 34.502724707457624 38.0 34.0 40.0 24.5 41.0 60-61 34.20614839145824 38.0 34.0 40.0 23.5 41.0 62-63 33.88748837872224 37.0 33.0 40.0 23.0 41.0 64-65 33.52499506381733 37.0 33.0 40.0 22.0 41.0 66-67 33.12522735861269 36.0 32.5 39.0 21.5 41.0 68-69 32.63469959895968 36.0 32.0 39.0 19.5 40.5 70-71 32.19618122524553 35.0 31.5 38.5 18.5 40.0 72-73 31.77592574673656 35.0 31.0 37.5 17.5 40.0 74-75 31.317263498007875 35.0 31.0 37.0 13.5 39.0 76-77 30.879337793114452 35.0 30.5 36.5 9.5 39.0 78-79 30.404353778500735 34.0 30.0 36.0 7.0 38.5 80-81 29.94464479752498 34.0 29.5 36.0 4.5 37.5 82-83 29.50273091854841 34.0 29.0 35.0 2.0 37.0 84-85 29.03579811208992 34.0 29.0 35.0 2.0 36.5 86-87 28.5951460652413 34.0 29.0 35.0 2.0 36.0 88-89 28.210484386625367 34.0 27.5 35.0 2.0 36.0 90-91 27.759023734212388 33.5 27.0 35.0 2.0 35.0 92-93 27.301466732743954 33.0 26.0 35.0 2.0 35.0 94-95 26.746757810610113 33.0 25.0 35.0 2.0 35.0 96-97 26.085946459089833 32.5 24.5 35.0 2.0 35.0 98-99 25.34356256399058 32.0 21.5 34.0 2.0 35.0 100-101 23.46902888614874 30.5 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12916921878813525 1101 2 0.17244781967319156 1101 3 0.2790251196712248 1101 4 0.15464323754015652 1101 5 0.22589129362622629 1101 6 0.12126402959006555 1101 7 0.19285778153420807 1101 8 0.17521858213860497 1101 9 0.2236863350343299 1101 10-11 0.24813378375070982 1101 12-13 0.32482422898257113 1101 14-15 0.40380315663310284 1101 16-17 0.25889330545283684 1101 18-19 0.46192064890001916 1101 20-21 0.5698768236317022 1101 22-23 0.368826014854136 1101 24-25 0.5644993926715713 1101 26-27 0.5439726494894188 1101 28-29 0.5418206893930133 1101 30-31 0.4757101176649172 1101 32-33 0.2550609691558918 1101 34-35 0.24367185610856268 1101 36-37 0.5509733672193988 1101 38-39 0.5492465525051315 1101 40-41 0.481148812729181 1101 42-43 0.3824224249093362 1101 44-45 0.27922587090793627 1101 46-47 0.3843354124653757 1101 48-49 0.3983568097353327 1101 50-51 0.5667056599635387 1101 52-53 0.4911273118769728 1101 54-55 0.4272204002598059 1101 56-57 0.5383074599215476 1101 58-59 0.4720188654417612 1101 60-61 0.36603791034416844 1101 62-63 0.5398580442437293 1101 64-65 0.4968568665959836 1101 66-67 0.459697923807191 1101 68-69 0.4320505869763309 1101 70-71 0.4146446799707135 1101 72-73 0.39769682594780065 1101 74-75 0.47367535870375477 1101 76-77 0.6320104045796313 1101 78-79 0.6125450976102726 1101 80-81 0.7328135647466283 1101 82-83 0.6047074171715892 1101 84-85 0.5347020889090928 1101 86-87 0.7993584465638861 1101 88-89 0.9240410514577349 1101 90-91 0.7947718930492691 1101 92-93 0.681034551914621 1101 94-95 0.716392519024712 1101 96-97 0.6377041730140114 1101 98-99 0.7459807308699915 1101 100-101 1.2181097907420053 1102 1 -0.01465149223253448 1102 2 -0.10165732998739685 1102 3 0.017404593922922373 1102 4 -0.08943791783046606 1102 5 -0.10365661142927962 1102 6 -0.08996620928979837 1102 7 -0.033781954832328154 1102 8 0.15541038459573997 1102 9 0.10911587838351267 1102 10-11 -0.030122529936669196 1102 12-13 0.1815714840661684 1102 14-15 0.22214360219795992 1102 16-17 0.2913277387775608 1102 18-19 0.2777162570337808 1102 20-21 0.2192181196270866 1102 22-23 0.23964371899543124 1102 24-25 0.30565225755941583 1102 26-27 0.3325878904194255 1102 28-29 0.41664946431745875 1102 30-31 0.37183344527809936 1102 32-33 0.103646748742527 1102 34-35 0.1415089531031981 1102 36-37 0.21687869607784194 1102 38-39 0.35324661325016393 1102 40-41 0.20499541632224094 1102 42-43 -0.0019991304673538934 1102 44-45 0.14758795689253645 1102 46-47 0.38364102075099993 1102 48-49 0.3006537314805371 1102 50-51 0.2560179502033222 1102 52-53 0.1822699260396874 1102 54-55 0.42198721524560767 1102 56-57 0.3855021781405057 1102 58-59 0.2954865718609909 1102 60-61 0.4289242108195026 1102 62-63 0.3905311751948446 1102 64-65 0.3751074854360539 1102 66-67 0.5333850716757951 1102 68-69 0.40832964968039676 1102 70-71 0.2689245475465256 1102 72-73 0.3588545504386822 1102 74-75 0.48125671908887924 1102 76-77 0.44553111104442067 1102 78-79 0.3975289242439324 1102 80-81 0.4297250592264241 1102 82-83 0.334669907110694 1102 84-85 0.4925207345984717 1102 86-87 0.4820381379790959 1102 88-89 0.517452113129135 1102 90-91 0.5039360413279077 1102 92-93 0.35643470985172243 1102 94-95 0.23429446134730014 1102 96-97 0.5901377710944686 1102 98-99 0.6976671649844022 1102 100-101 0.9377558749650028 1103 1 0.25548430897953267 1103 2 0.21899870941418698 1103 3 0.3214869269432441 1103 4 0.049806407443796274 1103 5 0.19294779258780892 1103 6 0.2681718697321074 1103 7 0.2128192436576768 1103 8 0.3454068773719925 1103 9 0.42669046345631045 1103 10-11 0.3851759423468408 1103 12-13 0.3455847285498308 1103 14-15 0.44114822947057775 1103 16-17 0.5087201744878911 1103 18-19 0.48115748463751373 1103 20-21 0.4098694827303362 1103 22-23 0.4584018978034976 1103 24-25 0.26942169342436273 1103 26-27 0.16519828311534468 1103 28-29 -0.07072000473412032 1103 30-31 0.01982659676376386 1103 32-33 0.12099543843618932 1103 34-35 0.22552242385936694 1103 36-37 0.43711405718990193 1103 38-39 0.20362184847927978 1103 40-41 0.34442415368398116 1103 42-43 0.44758999638477803 1103 44-45 0.22150571438121602 1103 46-47 0.35648285250231027 1103 48-49 0.18668574149687345 1103 50-51 0.19985636146868302 1103 52-53 0.24217500472594367 1103 54-55 0.19447040508446634 1103 56-57 0.20616934260667108 1103 58-59 0.3183685669810501 1103 60-61 0.41259770714446375 1103 62-63 0.4962841535066289 1103 64-65 0.49850745602063284 1103 66-67 0.7116828990826747 1103 68-69 0.413884639554702 1103 70-71 0.458610481316569 1103 72-73 0.496814025033931 1103 74-75 0.43595489042995794 1103 76-77 0.38096067052957494 1103 78-79 0.7294227873566115 1103 80-81 0.543207694697653 1103 82-83 0.7358401130563799 1103 84-85 0.5257471541755692 1103 86-87 0.8656267821165642 1103 88-89 0.8129227033609503 1103 90-91 0.9070320148087738 1103 92-93 0.8597782631596367 1103 94-95 0.9550889040947013 1103 96-97 0.9377228778385209 1103 98-99 1.0396646609020692 1103 100-101 1.2110589254954185 1104 1 0.08822002664982165 1104 2 0.08573600174860019 1104 3 0.006718415181008908 1104 4 0.0835535855700158 1104 5 0.08320775630207322 1104 6 0.027813984970855188 1104 7 -0.11742277537161527 1104 8 0.04528634530127107 1104 9 0.1827930076018589 1104 10-11 0.2130765042893259 1104 12-13 0.14505269386398112 1104 14-15 0.18798764075519614 1104 16-17 0.22613246443508217 1104 18-19 0.3508588930070218 1104 20-21 0.36714834061239543 1104 22-23 0.31884179481974684 1104 24-25 0.2629803442579117 1104 26-27 0.32915054592930915 1104 28-29 0.24619772537133855 1104 30-31 0.3304083757018219 1104 32-33 0.12131451511368141 1104 34-35 0.19553516170301322 1104 36-37 0.12972772938231003 1104 38-39 0.2949195771073576 1104 40-41 0.3128137623421168 1104 42-43 0.20468940741715613 1104 44-45 0.3175485149772044 1104 46-47 0.4257821269677109 1104 48-49 0.5246589289665735 1104 50-51 0.25444027359016985 1104 52-53 0.28380613334933713 1104 54-55 0.39392539833719553 1104 56-57 0.45950829716662156 1104 58-59 0.3329554698833661 1104 60-61 0.37232414078246023 1104 62-63 0.505137585427633 1104 64-65 0.3294820361852686 1104 66-67 0.4035308593160991 1104 68-69 0.5319671526754064 1104 70-71 0.26857897001736575 1104 72-73 0.4540256804311191 1104 74-75 0.33913772983403234 1104 76-77 0.5470378290715878 1104 78-79 0.47765867708688603 1104 80-81 0.6148873424341197 1104 82-83 0.5668224404243674 1104 84-85 0.6106022724645541 1104 86-87 0.47907538662903804 1104 88-89 0.3884282083795192 1104 90-91 0.6992352653911631 1104 92-93 0.6513422793819181 1104 94-95 0.6692392610966387 1104 96-97 0.8680742585523085 1104 98-99 0.9377624522284442 1104 100-101 1.0018148903915822 1105 1 -0.0059374592543086635 1105 2 0.16912025462174185 1105 3 0.1062819097127985 1105 4 0.24400136041661824 1105 5 0.1658986496930197 1105 6 0.16159577614341458 1105 7 0.1740666486094753 1105 8 0.19986818916184035 1105 9 0.3117443334003198 1105 10-11 0.27386418993221895 1105 12-13 0.2928381512434086 1105 14-15 0.33869819701610027 1105 16-17 0.36047900513839437 1105 18-19 0.3167540219279772 1105 20-21 0.1964573228192137 1105 22-23 0.34187047685131233 1105 24-25 0.49664834397982816 1105 26-27 0.4984777128149034 1105 28-29 0.32799731338042193 1105 30-31 0.2506939885840396 1105 32-33 0.1430938694661137 1105 34-35 0.3496011022021577 1105 36-37 0.354575428124015 1105 38-39 0.11588226741532992 1105 40-41 0.18141207852135466 1105 42-43 0.17487092490481615 1105 44-45 0.1631744960168504 1105 46-47 0.07297594198158919 1105 48-49 0.18586445974332833 1105 50-51 0.07263611196972164 1105 52-53 0.0260641069833909 1105 54-55 0.01161393174467662 1105 56-57 0.25330245538430773 1105 58-59 0.26598078573696426 1105 60-61 0.22109475404885615 1105 62-63 0.2207641518895187 1105 64-65 0.09563731212038817 1105 66-67 0.1465732987706332 1105 68-69 0.3487566970339415 1105 70-71 0.40061803231726856 1105 72-73 0.2732183831427264 1105 74-75 0.009644339617171482 1105 76-77 0.1485559021542393 1105 78-79 0.27553322841560757 1105 80-81 0.3031774337965807 1105 82-83 0.20252953903508342 1105 84-85 0.24698114311184582 1105 86-87 0.22686011223064995 1105 88-89 0.522862196532035 1105 90-91 0.6730930516900848 1105 92-93 0.672628964318637 1105 94-95 0.4981792443389317 1105 96-97 0.4961375719690899 1105 98-99 0.5168716590910556 1105 100-101 0.7636808506487291 1106 1 0.13186697785572932 1106 2 0.13002513704975982 1106 3 -0.06395641730079049 1106 4 0.11201680593546826 1106 5 0.20716936655917095 1106 6 0.23425739319467453 1106 7 0.20265152015949894 1106 8 0.14081411130381838 1106 9 0.09234024364448601 1106 10-11 -0.017065430666754366 1106 12-13 -0.0031947794186351075 1106 14-15 -0.00789617114845953 1106 16-17 -0.07851807140004752 1106 18-19 0.13922056127263716 1106 20-21 0.09760430247551 1106 22-23 0.13686775735219214 1106 24-25 0.12007690613738475 1106 26-27 0.3064273804488451 1106 28-29 0.41441017043056405 1106 30-31 0.3430549773691922 1106 32-33 0.5367730427383819 1106 34-35 0.43655090581105327 1106 36-37 0.04066642627290662 1106 38-39 0.03954932655516785 1106 40-41 0.3537804223154879 1106 42-43 0.3657762863355174 1106 44-45 0.3126391718865449 1106 46-47 0.3322407810212411 1106 48-49 0.3034542109400604 1106 50-51 0.06477081061522227 1106 52-53 0.2846154637971168 1106 54-55 0.3538876184274784 1106 56-57 0.21920055451622744 1106 58-59 0.14469382913461004 1106 60-61 0.485492261380692 1106 62-63 0.6949416579234082 1106 64-65 0.44623367046384743 1106 66-67 0.45202133625027585 1106 68-69 0.49734294378522037 1106 70-71 0.41598513379475577 1106 72-73 0.6029348599080073 1106 74-75 0.4116751564867194 1106 76-77 0.27305372191943533 1106 78-79 0.17939391280228278 1106 80-81 0.2664336786009436 1106 82-83 0.22682965430476543 1106 84-85 0.13990550086195697 1106 86-87 0.1552619583997128 1106 88-89 0.274404276145372 1106 90-91 0.498024523402659 1106 92-93 0.4553205113118928 1106 94-95 0.5148763508536724 1106 96-97 0.8293063447024025 1106 98-99 0.9600159717840135 1106 100-101 1.0752817548821874 1107 1 -0.025525748673583593 1107 2 -0.08579348806286902 1107 3 -0.1496067213085439 1107 4 -0.06714522300394776 1107 5 -0.01222466231967445 1107 6 0.08035148037637896 1107 7 -0.1544833865937889 1107 8 -0.044792276266321096 1107 9 -0.07850492062570424 1107 10-11 -0.05466982685359767 1107 12-13 0.014972996516675607 1107 14-15 0.13003986986041127 1107 16-17 0.0012900213393507443 1107 18-19 0.13840943224160185 1107 20-21 0.14795374102656922 1107 22-23 0.14820520217270428 1107 24-25 0.19831612200182036 1107 26-27 0.1398536576221403 1107 28-29 0.13131610596426668 1107 30-31 0.0038088257363355638 1107 32-33 -0.1404244827975134 1107 34-35 0.07910441950465952 1107 36-37 0.03910852850734159 1107 38-39 -0.013078845082382884 1107 40-41 0.07104033947999966 1107 42-43 0.17326498542463753 1107 44-45 0.2022285153358041 1107 46-47 0.1839459502888232 1107 48-49 0.06963817538289163 1107 50-51 0.05644391331154708 1107 52-53 0.11384279950799936 1107 54-55 0.0757814769408256 1107 56-57 0.24533310643983697 1107 58-59 0.11287693796408149 1107 60-61 0.10135764235578648 1107 62-63 0.22875901854550307 1107 64-65 0.3318281324496226 1107 66-67 0.2679575135515293 1107 68-69 0.37231606389668315 1107 70-71 0.3593478835694981 1107 72-73 0.30001347033133996 1107 74-75 0.2561314634171872 1107 76-77 0.19660258652808338 1107 78-79 0.43209761314074413 1107 80-81 0.35232268359076 1107 82-83 0.40687909815608236 1107 84-85 0.35196510606832376 1107 86-87 0.3466935206891222 1107 88-89 0.6000242172367685 1107 90-91 0.5136928482934344 1107 92-93 0.44812749099675386 1107 94-95 0.38192539339961584 1107 96-97 0.6517654380551186 1107 98-99 0.8181725035969265 1107 100-101 1.1486704759295172 1108 1 -0.13776659553390402 1108 2 -0.26801699936786605 1108 3 -0.15265972384684545 1108 4 -0.15302496052284909 1108 5 -0.01902945457802474 1108 6 0.03186322798141816 1108 7 -0.01320095787242792 1108 8 0.03929992072192334 1108 9 0.02843533709398116 1108 10-11 -0.1141606242556108 1108 12-13 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pass #Quality Count 2 6068.0 3 1180.0 4 1109.0 5 1311.0 6 1527.0 7 1781.0 8 2221.0 9 2754.0 10 3416.0 11 4224.0 12 5579.0 13 6926.0 14 7974.0 15 9418.0 16 11505.0 17 14709.0 18 12713.0 19 9648.0 20 9878.0 21 10475.0 22 11308.0 23 12971.0 24 14418.0 25 16294.0 26 19242.0 27 22815.0 28 26946.0 29 32542.0 30 39861.0 31 48842.0 32 61664.0 33 79802.0 34 107100.0 35 148081.0 36 212502.0 37 290824.0 38 230627.0 39 29264.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62752942966657 19.329460099747354 14.651802509687457 22.39120796089862 2 15.042794331504941 20.28872241185102 40.20129792643859 24.46718533020544 3 19.34742969467646 24.86380197866597 29.51974502497756 26.26902330168001 4 12.520743983671018 16.30813151046362 37.80850426288455 33.36262024298081 5 13.669143041456769 37.752368639812026 33.88138313355379 14.697105185177413 6 31.591630233315403 36.344225229197605 17.647282354814216 14.416862182672771 7 28.566046389726118 32.88867776176124 20.507338487747344 18.037937360765298 8 26.561842748397694 34.64601288655403 19.68265845128099 19.10948591376728 9 26.38188468990026 17.003381452917477 20.416414690221306 36.19831916696096 10-11 24.85057651272135 27.01273265359011 27.184639694702007 20.952051138986533 12-13 25.993235149961603 24.13627595136437 27.834107844447765 22.03638105422626 14-15 22.812496621910462 25.477690523431086 26.431188029459236 25.27862482519922 16-17 21.976693177950153 29.150727080986144 26.71950140397214 22.153078337091564 18-19 21.883756322896335 27.66213508019211 29.18346275575751 21.27064584115404 20-21 22.75565933403567 26.44572464109942 29.214264561745573 21.58435146311933 22-23 22.345522866471367 26.489164841234043 29.19794634325471 21.967365949039884 24-25 22.29303827735524 26.611376425656246 29.31715897254486 21.778426324443657 26-27 22.04718966773953 26.915571279984928 29.2011170896702 21.836121962605343 28-29 21.767534165484875 27.1745208707813 29.282810642445757 21.77513432128807 30-31 21.949945009816986 26.80683784988876 29.25766037656727 21.985556763726986 32-33 21.769577565750524 26.951875827440848 29.206053205859256 22.072493400949373 34-35 22.34618442505208 26.833589232639493 29.007122703580812 21.813103638727615 36-37 22.160917537100016 26.671785642452207 29.127976796888255 22.03932002355952 38-39 22.009355452494106 27.017447548771216 29.1372131940639 21.835983804670782 40-41 22.190508134368738 26.945219281408413 29.01772436825493 21.846548215967914 42-43 21.91773777404665 26.988827029737706 28.746610293570935 22.346824902644716 44-45 22.08338110853762 27.128803042761813 28.537554586583415 22.250261262117156 46-47 22.259048497562215 27.295585782364356 28.23528910773553 22.2100766123379 48-49 22.3268297508922 27.305939184439097 28.147130913124464 22.220100151544237 50-51 22.17963502932215 27.471480522884384 28.119156046260198 22.22972840153327 52-53 22.40209402538308 27.621135790560764 27.76300476522487 22.213765418831287 54-55 22.145861100517532 27.40232831525436 27.959801635822977 22.492008948405132 56-57 22.268552651015053 27.67427761596154 27.776400347308993 22.28076938571441 58-59 22.619738457124967 27.308673252178107 27.803601814530655 22.267986476166275 60-61 22.400019912992015 27.358846199320535 28.32069611627877 21.920437771408675 62-63 22.286095893259933 27.456719055668238 28.178245534510943 22.078939516560883 64-65 22.167738884345578 27.53529017017164 28.073489623948156 22.223481321534628 66-67 22.04860974631899 27.62040086892493 27.788270299590433 22.54271908516565 68-69 22.00819317767955 27.55751862264482 28.051087489093668 22.38320071058196 70-71 22.228441670184896 27.461075408422854 27.901276944739312 22.40920597665294 72-73 22.172839037058463 27.35124907829641 28.016513521649678 22.459398362995454 74-75 21.962604271888416 27.753190552740907 27.871562974521435 22.41264220084924 76-77 22.10529419086865 27.53070971376909 27.83692241170343 22.52707368365883 78-79 22.06480882461327 27.59493256246264 27.857246976161427 22.48301163676267 80-81 22.127251074934954 27.750025546900183 27.750484081005943 22.37223929715892 82-83 22.187580049515233 27.671991399365435 27.874723450421545 22.265705100697787 84-85 22.100027805698105 27.607455595217157 27.627859540940698 22.664657058144037 86-87 22.046661253761222 27.550727974644285 27.841570655283626 22.56104011631087 88-89 22.168493402852157 27.465209470334944 27.646081573908297 22.720215552904598 90-91 22.151112840533198 27.377210212892518 27.828595928717675 22.643081017856613 92-93 22.33279548019934 27.275344773030564 27.837240793769023 22.55461895300107 94-95 22.063610138551645 27.17507478853997 27.882425503801088 22.878889569107297 96-97 21.88507778584965 27.267757701450908 27.975851635340305 22.87131287735914 98-99 21.869108061187724 27.292574273641456 27.712568377608175 23.125749287562645 100-101 21.809123662434118 27.125163055884244 28.109213300185598 22.95649998149604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2096.0 1 1203.5 2 326.0 3 343.0 4 294.5 5 302.5 6 504.5 7 937.5 8 1540.0 9 1872.5 10 1769.0 11 1638.0 12 1729.5 13 1876.5 14 1948.5 15 2031.0 16 2079.5 17 2187.5 18 2389.0 19 2476.5 20 2681.5 21 3105.0 22 3683.5 23 4427.5 24 5298.0 25 6495.5 26 7948.0 27 9832.0 28 11825.5 29 14271.0 30 17337.0 31 20378.5 32 23478.5 33 26506.0 34 30143.0 35 33088.0 36 36407.5 37 40354.0 38 43244.0 39 44741.0 40 46043.5 41 48668.5 42 50964.5 43 53576.0 44 56395.5 45 58386.5 46 58945.5 47 61177.0 48 67358.5 49 66761.5 50 60005.5 51 55720.5 52 52088.5 53 49453.0 54 47328.5 55 43868.5 56 39917.5 57 35999.5 58 32342.5 59 28484.5 60 23874.0 61 19199.5 62 14980.5 63 11257.0 64 8462.0 65 6645.5 66 4986.0 67 3710.0 68 2668.0 69 1775.5 70 1271.5 71 939.5 72 692.0 73 558.0 74 424.5 75 294.0 76 188.5 77 114.0 78 75.0 79 51.0 80 31.5 81 20.0 82 15.0 83 11.5 84 6.5 85 5.0 86 5.5 87 5.5 88 6.5 89 5.0 90 2.5 91 3.0 92 3.0 93 1.5 94 1.5 95 1.5 96 0.5 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31840012762157066 2 0.28244118097026394 3 0.2845987177693423 4 0.28642935505340883 5 0.27289571513191707 6 0.21045790776464804 7 0.2171920377738928 8 0.21444608184779299 9 0.21281158427273356 10-11 0.2227166395775935 12-13 0.22216091040207334 14-15 0.2053909652819639 16-17 0.2168324483073797 18-19 0.20431219688242472 20-21 0.20882341018958864 22-23 0.20735236237203516 24-25 0.19823186590320374 26-27 0.21147129626118485 28-29 0.211667435970192 30-31 0.21840156597943672 32-33 0.21938226452447232 34-35 0.209575279074116 36-37 0.20758119203254352 38-39 0.19826455585470493 40-41 0.19849338551521326 42-43 0.19780689653368827 44-45 0.21424994213878587 46-47 0.20503137581545086 48-49 0.21150398621268604 50-51 0.22046103292401156 52-53 0.2266067438062349 54-55 0.2249068663281731 56-57 0.19139966603945546 58-59 0.17887941461450046 60-61 0.1883268105983438 62-63 0.18943826894938418 64-65 0.18682307282928914 66-67 0.19862414532121803 68-69 0.18868640006485687 70-71 0.19277264400250535 72-73 0.20506406576695202 74-75 0.1950936305590897 76-77 0.20476985620344135 78-79 0.2032988083858879 80-81 0.19077855696093288 82-83 0.20418143707641995 84-85 0.18675769292628677 86-87 0.19113814642744595 88-89 0.19803572619419663 90-91 0.1961723989586289 92-93 0.20156624095632492 94-95 0.19950677401175007 96-97 0.1934591329840303 98-99 0.20084706202329877 100-101 0.18446939632120363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1529522.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.795294544962594 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44115995205057 47.502075583263505 2 11.945570189411852 14.285777759668125 3 3.662271875306284 6.569598764630154 4 1.6307801060768918 3.900519071237323 5 0.869887112677995 2.600757806172389 6 0.532721161920694 1.9112531272429563 7 0.347122871335141 1.4529420034344538 8 0.23094356228522397 1.1047470664086305 9 0.19190207852422494 1.0327357178231913 >10 1.041014638138554 11.286049107544924 >50 0.07192144224540344 2.9352798222205267 >100 0.03262500341200892 3.440380598181181 >500 0.001094740323795851 0.4406788749914301 >1k 8.757922590366809E-4 1.146930268675705 >5k 1.0947403237958511E-4 0.3902744285054822 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5962 0.38979498170016513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4216 0.27564167105801685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2982 0.19496287075308497 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2761 0.18051391218955987 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2271 0.1484777597183957 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1655 0.10820373946893212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 6.53799030023759E-5 9 0.0 0.0 0.0 0.0 6.53799030023759E-5 10-11 6.53799030023759E-5 0.0 0.0 0.0 6.53799030023759E-5 12-13 6.53799030023759E-5 0.0 0.0 0.0 6.53799030023759E-5 14-15 6.53799030023759E-5 0.0 0.0 0.0 6.53799030023759E-5 16-17 6.53799030023759E-5 0.0 0.0 0.0 1.307598060047518E-4 18-19 9.806985450356385E-5 0.0 0.0 0.0 1.961397090071277E-4 20-21 1.307598060047518E-4 0.0 0.0 0.0 1.961397090071277E-4 22-23 1.307598060047518E-4 0.0 0.0 0.0 1.961397090071277E-4 24-25 1.307598060047518E-4 0.0 0.0 1.307598060047518E-4 1.961397090071277E-4 26-27 1.307598060047518E-4 0.0 0.0 2.615196120095036E-4 1.961397090071277E-4 28-29 1.307598060047518E-4 0.0 0.0 6.53799030023759E-4 1.961397090071277E-4 30-31 1.307598060047518E-4 0.0 0.0 0.0014383578660522697 1.961397090071277E-4 32-33 1.6344975750593976E-4 0.0 0.0 0.002909405683605728 1.961397090071277E-4 34-35 1.961397090071277E-4 0.0 0.0 0.00431507359815681 1.961397090071277E-4 36-37 2.615196120095036E-4 0.0 0.0 0.006047641027719771 1.961397090071277E-4 38-39 2.942095635106916E-4 0.0 0.0 0.009545465838346883 1.961397090071277E-4 40-41 3.268995150118795E-4 0.0 0.0 0.01340288011548706 1.961397090071277E-4 42-43 3.268995150118795E-4 0.0 0.0 0.016639185314104667 1.961397090071277E-4 44-45 3.268995150118795E-4 0.0 0.0 0.018469822598171193 1.961397090071277E-4 46-47 3.922794180142554E-4 0.0 0.0 0.020954258912261478 1.961397090071277E-4 48-49 4.576593210166313E-4 0.0 0.0 0.02504050284990997 2.615196120095036E-4 50-51 4.576593210166313E-4 0.0 0.0 0.03023820513859886 2.615196120095036E-4 52-53 4.576593210166313E-4 0.0 0.0 0.04413143452660374 2.615196120095036E-4 54-55 5.230392240190072E-4 0.0 0.0 0.05171550327487934 2.615196120095036E-4 56-57 5.884191270213832E-4 0.0 0.0 0.0754157181132406 2.615196120095036E-4 58-59 5.884191270213832E-4 0.0 0.0 0.1204297813303764 2.942095635106916E-4 60-61 5.884191270213832E-4 0.0 0.0 0.16070380157984 3.268995150118795E-4 62-63 5.884191270213832E-4 0.0 0.0 0.18443670636970244 3.268995150118795E-4 64-65 5.884191270213832E-4 0.0 0.0 0.2276528222542729 3.268995150118795E-4 66-67 5.884191270213832E-4 0.0 0.0 0.3117640674668295 3.5958946651306744E-4 68-69 6.53799030023759E-4 0.0 0.0 0.42836912447156694 3.922794180142554E-4 70-71 6.53799030023759E-4 0.0 0.0 0.5447126618642948 3.922794180142554E-4 72-73 6.86488981524947E-4 0.0 0.0 0.6667442508182295 4.576593210166313E-4 74-75 7.19178933026135E-4 0.0 0.0 0.8140778622340836 4.576593210166313E-4 76-77 7.19178933026135E-4 0.0 0.0 0.9168550697538185 4.576593210166313E-4 78-79 7.19178933026135E-4 0.0 0.0 1.054512455525321 4.576593210166313E-4 80-81 8.499387390308868E-4 0.0 0.0 1.2353532672298928 4.576593210166313E-4 82-83 8.499387390308868E-4 0.0 0.0 1.4005682821168968 4.576593210166313E-4 84-85 9.153186420332626E-4 0.0 0.0 1.602559492442737 4.576593210166313E-4 86-87 9.806985450356385E-4 0.0 0.0 1.8792145519972907 4.576593210166313E-4 88-89 9.806985450356385E-4 0.0 0.0 2.2395558873948853 4.576593210166313E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10130 0.0 25.863613 1 GTACATG 7115 0.0 24.192495 1 GGTATCA 7375 0.0 23.017239 1 TACATGG 7375 0.0 22.685934 2 GAGTACT 5265 0.0 22.59885 12-13 ATCAACG 11750 0.0 22.127151 3 TCAACGC 11825 0.0 21.946615 4 TATCAAC 11905 0.0 21.918913 2 CAACGCA 11900 0.0 21.806866 5 ACATGGG 7430 0.0 21.750345 3 AACGCAG 12060 0.0 21.585045 6 AGTACTT 5495 0.0 21.30719 12-13 GTACTTT 5555 0.0 20.863972 14-15 ATTCGCG 185 0.0 20.536388 80-81 AGAGTAC 9690 0.0 19.75447 10-11 ACGCAGA 13395 0.0 19.362865 7 CGCAGAG 13460 0.0 19.233437 8 CATGGGG 3260 0.0 18.516638 4 TACTTTT 6240 0.0 18.459433 14-15 CATGGGA 4305 0.0 18.438234 4 >>END_MODULE