FastQCFastQC Report
Thu 26 May 2016
SRR937245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529522
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79860.522123905376974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62680.4098012320188922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.28754081340444926No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31880.20843113077157438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111450.025.759861
ACCGTCG950.001226592825.0005158
TACCGTG1551.4108919E-624.5166367
GGTATCA81200.023.7074931
GTACATG68350.022.670741
GAGTACT67450.022.4300412-13
ATCAACG131000.021.7597313
TCAACGC131400.021.6934934
CAACGCA132400.021.6372915
TACATGG70250.021.5733662
TATCAAC133050.021.5672932
AACGCAG134750.021.2903276
TGTACGG1805.1623574E-621.1149985
GTACTTT71400.021.05473114-15
AGTACTT69400.020.94423712-13
TTCGGCG1150.003719863720.6559754
GTACCGT1856.5569693E-620.5396216
CCATGCG1404.8520707E-420.3575639
ACATGGG71500.020.1995223
ACGGTAC1200.00476363719.7920768
Produced by FastQC (version 0.11.5)