##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937245_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1529522 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14681776398117 33.0 31.0 34.0 31.0 34.0 2 32.375465014560106 34.0 31.0 34.0 31.0 34.0 3 32.45875443439192 34.0 31.0 34.0 31.0 34.0 4 36.06721054028644 37.0 35.0 37.0 35.0 37.0 5 35.96229671753659 37.0 35.0 37.0 35.0 37.0 6 36.04021517833676 37.0 35.0 37.0 35.0 37.0 7 35.99586995152734 37.0 35.0 37.0 35.0 37.0 8 35.9796263146264 37.0 35.0 37.0 35.0 37.0 9 37.67491412349741 39.0 37.0 39.0 35.0 39.0 10-11 37.60388605067466 39.0 37.0 39.0 35.0 39.0 12-13 37.523815610367166 39.0 37.0 39.0 35.0 39.0 14-15 38.736009027657005 40.0 38.0 41.0 35.0 41.0 16-17 38.726111491040996 40.0 38.0 41.0 35.0 41.0 18-19 38.72725073585081 40.0 38.0 41.0 34.5 41.0 20-21 38.62167821057821 40.0 38.0 41.0 34.0 41.0 22-23 38.503700502509936 40.0 38.0 41.0 34.0 41.0 24-25 38.36276529530141 40.0 38.0 41.0 34.0 41.0 26-27 38.32119642607298 40.0 38.0 41.0 34.0 41.0 28-29 38.23196397305825 40.0 38.0 41.0 34.0 41.0 30-31 38.30654577050869 40.0 38.0 41.0 34.0 41.0 32-33 38.23604465970415 40.0 38.0 41.0 34.0 41.0 34-35 38.12631789539477 40.0 37.5 41.0 34.0 41.0 36-37 37.97286112916323 40.0 37.0 41.0 33.0 41.0 38-39 37.84713492189063 40.0 37.0 41.0 33.0 41.0 40-41 37.690436620068226 40.0 37.0 41.0 33.0 41.0 42-43 37.489148570599184 40.0 37.0 41.0 33.0 41.0 44-45 37.20901399260684 40.0 37.0 41.0 32.0 41.0 46-47 36.861928105643464 40.0 36.0 41.0 31.0 41.0 48-49 36.55787363633867 39.5 36.0 41.0 30.5 41.0 50-51 36.34293262862515 39.0 36.0 41.0 30.0 41.0 52-53 36.124004754426544 39.0 35.0 41.0 30.0 41.0 54-55 35.90032278058112 39.0 35.0 40.0 29.0 41.0 56-57 35.65432174234826 39.0 35.0 40.0 28.5 41.0 58-59 35.40894540908859 38.0 35.0 40.0 28.0 41.0 60-61 35.07808289125622 38.0 34.0 40.0 28.0 41.0 62-63 34.715428414890404 37.5 34.0 40.0 27.0 41.0 64-65 34.36809114219998 37.0 34.0 40.0 26.0 41.0 66-67 33.93527781882183 36.5 33.5 39.0 26.0 41.0 68-69 33.54127498656443 36.0 33.0 39.0 26.0 40.0 70-71 33.134101372847205 35.5 33.0 38.5 25.0 40.0 72-73 32.69366834867364 35.0 32.0 38.0 24.0 40.0 74-75 32.25203200738531 35.0 32.0 37.0 23.0 39.0 76-77 30.915016259981876 34.0 30.5 35.5 21.5 38.5 78-79 31.562927502840758 34.5 31.0 36.0 22.0 38.5 80-81 31.41949870613172 35.0 31.0 36.0 22.0 37.5 82-83 31.097035544438064 34.0 31.0 35.5 20.5 37.0 84-85 30.737641563834977 34.0 31.0 35.0 19.5 36.5 86-87 30.371861928105645 34.0 31.0 35.0 18.0 36.0 88-89 29.982571025457627 34.0 30.0 35.0 15.5 36.0 90-91 29.537145918790316 34.0 30.0 35.0 8.5 35.0 92-93 29.17513216547392 34.0 29.0 35.0 4.5 35.0 94-95 28.76372781823341 34.0 29.0 35.0 2.0 35.0 96-97 28.292452478617506 34.0 29.0 35.0 2.0 35.0 98-99 27.802734449063173 33.5 29.0 35.0 2.0 35.0 100-101 25.86798130396294 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23579147065949968 1101 2 0.22122170440800915 1101 3 0.1730521131527425 1101 4 0.17059748012944453 1101 5 0.07425751174920947 1101 6 0.14894038832937895 1101 7 0.1469138663535432 1101 8 0.17598551382844363 1101 9 0.3039021185005737 1101 10-11 0.2705684623063007 1101 12-13 0.2546902985289563 1101 14-15 0.2759479161734575 1101 16-17 0.24207062156887815 1101 18-19 0.18829380405867369 1101 20-21 0.31121567315987164 1101 22-23 0.21547025419317833 1101 24-25 0.2852664591904812 1101 26-27 0.31456154427326766 1101 28-29 0.3602067501446271 1101 30-31 0.3145127468673792 1101 32-33 0.32347454733137937 1101 34-35 0.2946364508189845 1101 36-37 0.35284591245427777 1101 38-39 0.2140155101011061 1101 40-41 0.22037438382031382 1101 42-43 0.41847180657290295 1101 44-45 0.2837972814390213 1101 46-47 0.3203601124626232 1101 48-49 0.5220031911682241 1101 50-51 0.591189265337448 1101 52-53 0.5239204517552807 1101 54-55 0.3888625843336868 1101 56-57 0.5121536365041308 1101 58-59 0.5476896215600604 1101 60-61 0.542855734789903 1101 62-63 0.5247729261139185 1101 64-65 0.5811292856664068 1101 66-67 0.6397438874691161 1101 68-69 0.7233697751862636 1101 70-71 0.47601397072708806 1101 72-73 0.4803869368546074 1101 74-75 0.426348008617488 1101 76-77 0.4277234389191129 1101 78-79 0.4093552751066234 1101 80-81 0.6671435267396681 1101 82-83 0.8335850607977058 1101 84-85 0.6519782232297793 1101 86-87 0.4569557578809871 1101 88-89 0.42534124353506897 1101 90-91 0.532350746761761 1101 92-93 0.5043657274826749 1101 94-95 0.5345418714883188 1101 96-97 0.5310444923150932 1101 98-99 0.6113015829936046 1101 100-101 1.0479274674698438 1102 1 0.1027021298950288 1102 2 0.19412106506648286 1102 3 0.1166214915791457 1102 4 0.06876829517599248 1102 5 0.015163220264149402 1102 6 0.06671970160672913 1102 7 0.13732374349670096 1102 8 0.11432018861469828 1102 9 0.13395192943163892 1102 10-11 0.10105487819720338 1102 12-13 0.08544095533468266 1102 14-15 0.22688946426075063 1102 16-17 0.1734511284862279 1102 18-19 0.12430687942807594 1102 20-21 0.028871598079000194 1102 22-23 0.12018217085098826 1102 24-25 0.12084065166112623 1102 26-27 0.1282925804317543 1102 28-29 0.13401273049360896 1102 30-31 0.16501275446051267 1102 32-33 0.21331076352296208 1102 34-35 0.22111597214808398 1102 36-37 0.2661418622788787 1102 38-39 0.250312188866161 1102 40-41 0.358294853379455 1102 42-43 0.36495960441342135 1102 44-45 0.44106770316145116 1102 46-47 0.5367783572347449 1102 48-49 0.6029319068662673 1102 50-51 0.5052069421434737 1102 52-53 0.6593412630052669 1102 54-55 0.4805169754406151 1102 56-57 0.5006454890759287 1102 58-59 0.6200286092439953 1102 60-61 0.6745737563240155 1102 62-63 0.43791397609189886 1102 64-65 0.6118286888464084 1102 66-67 0.4693267209214085 1102 68-69 0.2943936327998884 1102 70-71 0.24203129824914527 1102 72-73 0.3603167763358428 1102 74-75 0.27119948699984064 1102 76-77 0.31607102202591975 1102 78-79 0.415372830422573 1102 80-81 0.47144275679631065 1102 82-83 0.45662337573035927 1102 84-85 0.33328241919327084 1102 86-87 0.13383733542962162 1102 88-89 0.24688067465779184 1102 90-91 0.3624707941428902 1102 92-93 0.4422865766983044 1102 94-95 0.507558166344733 1102 96-97 0.29307846397753323 1102 98-99 0.4662982420165207 1102 100-101 0.8787248220234467 1103 1 0.276401722981646 1103 2 0.23427462866980164 1103 3 0.15493960808685614 1103 4 0.12168325065687213 1103 5 0.1116812636104143 1103 6 0.05424906989161826 1103 7 0.03245963898897486 1103 8 0.10338481581189285 1103 9 0.18748574019994635 1103 10-11 0.1588171563087286 1103 12-13 0.18670902413660428 1103 14-15 0.16088615313983468 1103 16-17 0.12028161644287394 1103 18-19 0.19926266201037635 1103 20-21 0.2094412013855944 1103 22-23 0.16606929104317203 1103 24-25 0.19990101739291077 1103 26-27 0.21995651297238084 1103 28-29 -0.011847425558997315 1103 30-31 0.07274787987203979 1103 32-33 0.1871777982595617 1103 34-35 0.24363574270777377 1103 36-37 0.2296855913251008 1103 38-39 0.09082382870067818 1103 40-41 0.12246743141518124 1103 42-43 0.24927609175913545 1103 44-45 0.029903040892008903 1103 46-47 0.15602245623650646 1103 48-49 0.12306695383666266 1103 50-51 0.16649511148580842 1103 52-53 0.26327633034019016 1103 54-55 0.18074060809475156 1103 56-57 0.019283366338179064 1103 58-59 0.14991222975484675 1103 60-61 0.0590220474009584 1103 62-63 -0.0579118619648753 1103 64-65 -0.07954014664216658 1103 66-67 -0.01780157935728255 1103 68-69 0.02636046879381837 1103 70-71 -0.181093751247964 1103 72-73 -0.0755085714638426 1103 74-75 0.17402864749115565 1103 76-77 0.1591900753017761 1103 78-79 0.035200146793012976 1103 80-81 0.08949326836072302 1103 82-83 0.24538253505259533 1103 84-85 0.37373579385904776 1103 86-87 0.28616567945433147 1103 88-89 0.25436690031935427 1103 90-91 0.12104520196890078 1103 92-93 0.1818310901558604 1103 94-95 0.3769915800048018 1103 96-97 0.20115280957860904 1103 98-99 0.20907864078408878 1103 100-101 0.34163347958641666 1104 1 0.17031368232144217 1104 2 0.16664442610746022 1104 3 0.11048219158098505 1104 4 0.06234147467356621 1104 5 0.10119082960488157 1104 6 0.039910257664011795 1104 7 -0.03036153213593451 1104 8 0.0038736102930059246 1104 9 0.055930660286648504 1104 10-11 0.09694204483007951 1104 12-13 0.09462594528989143 1104 14-15 0.12459482946708533 1104 16-17 -0.0417026765527595 1104 18-19 0.010398996574252806 1104 20-21 -0.08379404874998642 1104 22-23 0.029103600343958647 1104 24-25 -0.04002338431580199 1104 26-27 -0.11146542314381946 1104 28-29 -0.110086648388382 1104 30-31 0.10187988681209248 1104 32-33 -0.027944742679004264 1104 34-35 0.040311651968202966 1104 36-37 -0.019498700229185317 1104 38-39 -0.049347762845144416 1104 40-41 -0.006001919387195187 1104 42-43 0.08283103114176527 1104 44-45 0.02118455444330891 1104 46-47 0.042073526521662075 1104 48-49 0.07604413201693916 1104 50-51 -0.17908817185103487 1104 52-53 -0.1346585895483159 1104 54-55 -0.1300512461713481 1104 56-57 -0.11609612951706794 1104 58-59 -0.13282447883680248 1104 60-61 -0.005449097869913544 1104 62-63 0.06054792194219516 1104 64-65 -0.1150673798636177 1104 66-67 -0.0018742301079512913 1104 68-69 0.03819351115659941 1104 70-71 0.025052843856165907 1104 72-73 -0.043024727986178846 1104 74-75 -0.07483512604547826 1104 76-77 0.04399276484251047 1104 78-79 0.20247956319438032 1104 80-81 0.18688408233956366 1104 82-83 0.23030972034948505 1104 84-85 0.1524099050476302 1104 86-87 0.3316376192639652 1104 88-89 0.2851393325268212 1104 90-91 0.2632872786517986 1104 92-93 0.07134642151095605 1104 94-95 0.22219867356038847 1104 96-97 0.3341063497802885 1104 98-99 0.44935941384688505 1104 100-101 0.9155808958940987 1105 1 0.038033865119537325 1105 2 0.049381421500918066 1105 3 -0.002228789111050844 1105 4 0.0357395215585683 1105 5 -0.14987189473075802 1105 6 -0.044345542589915965 1105 7 -0.024530726485146204 1105 8 -0.008969822184027976 1105 9 -0.08964519397248694 1105 10-11 -0.05208249533903597 1105 12-13 -0.07004839528681828 1105 14-15 -0.037548657527395335 1105 16-17 -0.0030134003907917872 1105 18-19 0.022845988418595198 1105 20-21 0.14745688884034536 1105 22-23 0.08754666985919357 1105 24-25 -0.005404073430966605 1105 26-27 0.029526391921287143 1105 28-29 -0.04516679635385401 1105 30-31 -0.1549629675604578 1105 32-33 -0.23379311099741074 1105 34-35 -0.1928944130063499 1105 36-37 -0.2607438933636459 1105 38-39 -0.22895311506810145 1105 40-41 -0.17332932044168814 1105 42-43 -0.35538679716120214 1105 44-45 -0.24852569812980363 1105 46-47 -0.4008988452068394 1105 48-49 -0.355187047572322 1105 50-51 -0.41547490910987506 1105 52-53 -0.460277947998712 1105 54-55 -0.4606847462280612 1105 56-57 -0.45726668131413106 1105 58-59 -0.5087810039166598 1105 60-61 -0.5874694998354713 1105 62-63 -0.5539712817371836 1105 64-65 -0.61931260592808 1105 66-67 -0.5519283816664498 1105 68-69 -0.43282680665450357 1105 70-71 -0.5798043314082832 1105 72-73 -0.5375012690187901 1105 74-75 -0.5560822218022636 1105 76-77 -0.5573767573431603 1105 78-79 -0.40505628477145805 1105 80-81 -0.48668117577676995 1105 82-83 -0.4919796831418246 1105 84-85 -0.4421696337128118 1105 86-87 -0.37985751258410616 1105 88-89 -0.5310635089664437 1105 90-91 -0.598638870330845 1105 92-93 -0.6958918855942393 1105 94-95 -0.6678012166729381 1105 96-97 -0.6472907604291116 1105 98-99 -0.6244087296693834 1105 100-101 0.02260669472105903 1106 1 -0.1564620849900109 1106 2 0.03177498246868993 1106 3 0.04763925337493902 1106 4 0.06803820909121328 1106 5 -0.037697191635167826 1106 6 -0.053368562531879604 1106 7 0.023152445686960732 1106 8 0.011023904425961462 1106 9 -0.0901790501913311 1106 10-11 -0.2208204623280281 1106 12-13 -0.2341678492855621 1106 14-15 -0.10561571172268458 1106 16-17 0.0951384990866373 1106 18-19 -0.01588871826179883 1106 20-21 -0.13157299165620628 1106 22-23 0.04385003616644667 1106 24-25 0.09216297921915384 1106 26-27 0.031842215388714123 1106 28-29 0.051191547100337687 1106 30-31 0.025236683849193753 1106 32-33 -0.023174471303228472 1106 34-35 -0.053510812204102365 1106 36-37 -0.03959530673060385 1106 38-39 -0.07768715732541409 1106 40-41 -0.06316913267262692 1106 42-43 -0.27535858235786037 1106 44-45 -0.07630453177371521 1106 46-47 0.020580253279206318 1106 48-49 0.20088330710598967 1106 50-51 0.17155215299668924 1106 52-53 0.24237765834143232 1106 54-55 0.0023323835260455894 1106 56-57 0.2565597312029766 1106 58-59 0.17010216269283518 1106 60-61 0.21809288022071627 1106 62-63 0.21990700213526537 1106 64-65 0.17186192410847667 1106 66-67 0.1824982366672927 1106 68-69 0.31641335602099474 1106 70-71 0.24371485496702405 1106 72-73 0.4006077210890311 1106 74-75 0.16050154649641257 1106 76-77 0.15886823244326465 1106 78-79 0.35998980764824395 1106 80-81 0.42492428670804827 1106 82-83 0.21768635927617908 1106 84-85 0.1765345713126969 1106 86-87 0.3028005042211639 1106 88-89 0.16117456154892906 1106 90-91 0.134185406761393 1106 92-93 0.1285729447924524 1106 94-95 -0.49092445324772527 1106 96-97 0.10035208280721619 1106 98-99 0.22015026337426136 1106 100-101 0.47901358741518507 1107 1 -0.059137580375001164 1107 2 -0.014567081149571948 1107 3 0.004593194118037047 1107 4 0.03982719818024805 1107 5 -0.056030789981484475 1107 6 -0.007784436052538979 1107 7 -0.02746768469943106 1107 8 -0.09574717735704752 1107 9 -0.007606682716755131 1107 10-11 -0.009191692518307093 1107 12-13 -0.03088168826367621 1107 14-15 -0.08569052110262732 1107 16-17 -0.10251291797565187 1107 18-19 -0.03748216562642881 1107 20-21 0.05714654131519126 1107 22-23 -0.01237398309621085 1107 24-25 0.01079017409455929 1107 26-27 0.01590868821730851 1107 28-29 -0.133349488267541 1107 30-31 -0.11361403055009589 1107 32-33 -0.12174018316597568 1107 34-35 0.0023285596394089225 1107 36-37 -0.03916484992393521 1107 38-39 0.06133562621955946 1107 40-41 0.022582444154636505 1107 42-43 0.0893511527048716 1107 44-45 -0.02008145898601299 1107 46-47 0.009559877566509556 1107 48-49 0.05345544742657893 1107 50-51 0.12549554622295744 1107 52-53 0.1950912020193556 1107 54-55 0.19593421287282098 1107 56-57 0.3123519685940366 1107 58-59 0.44133708534690896 1107 60-61 0.3851458979614435 1107 62-63 0.47868915388927036 1107 64-65 0.19503598463744964 1107 66-67 0.3909072879965265 1107 68-69 0.3784913228413629 1107 70-71 0.488517571728714 1107 72-73 0.4944688845717522 1107 74-75 0.34585749679511935 1107 76-77 0.35572593561357735 1107 78-79 0.36906763817622945 1107 80-81 0.3480054033970532 1107 82-83 0.17505050226204233 1107 84-85 0.3139095419719773 1107 86-87 0.1964210737719263 1107 88-89 0.33315227774273737 1107 90-91 0.35728980673413346 1107 92-93 0.34487237917902647 1107 94-95 0.3163204338945995 1107 96-97 0.3224314504105159 1107 98-99 0.47492352583554975 1107 100-101 1.0516320671492636 1108 1 -0.11442928378611583 1108 2 -0.04798949531845409 1108 3 -0.092258910458618 1108 4 -0.01622767203780029 1108 5 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2316 96-97 -0.16844352652516292 2316 98-99 -0.2295072689775921 2316 100-101 -0.6136394026759326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 14.0 5 38.0 6 66.0 7 145.0 8 323.0 9 669.0 10 1146.0 11 1787.0 12 2741.0 13 4003.0 14 5439.0 15 7558.0 16 10156.0 17 14389.0 18 12691.0 19 7619.0 20 6439.0 21 6466.0 22 6732.0 23 7570.0 24 8942.0 25 10517.0 26 13039.0 27 16081.0 28 20336.0 29 26005.0 30 32822.0 31 41768.0 32 54158.0 33 72167.0 34 99454.0 35 143371.0 36 220897.0 37 346830.0 38 296920.0 39 30213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99471558490324 19.87163803784526 15.322841259519862 21.81080511773164 2 15.765775189895928 21.00937417049248 39.419112637804496 23.8057380018071 3 19.806776234666778 24.883852602316278 29.871358502852523 25.438012660164418 4 13.060289423754611 16.978441630783998 37.514792203054284 32.44647674240711 5 13.65849862645357 37.85456036378545 33.8858253016342 14.601115708126777 6 31.735601057062272 36.71146933486409 17.118093103597072 14.43483650447656 7 28.536497023253016 33.10910205933619 20.378981145743573 17.975419771667227 8 26.790395953768563 34.75321048013693 19.43208401056016 19.024309555534344 9 26.480233693925292 16.855919692557546 20.262016499272324 36.401830114244845 10-11 25.054690288861487 27.128050462824334 26.907360600239816 20.909898648074364 12-13 26.215588907377317 24.19056087506579 27.883895559616473 21.70995465794042 14-15 22.8646269880394 25.5090479247765 26.64267006293469 24.98365502424941 16-17 21.844046702172314 29.11527261458155 26.98150141024451 22.059179273001632 18-19 21.741302184604077 27.40179611669528 29.833601608868655 21.023300089831988 20-21 22.609743436184637 26.135256635733256 30.084431606737265 21.170568321344838 22-23 22.2389814733997 26.182418019965304 29.99166069250577 21.586939814129224 24-25 22.173350632895257 26.348259272444235 30.084108168681844 21.394281925978667 26-27 22.00474338794277 26.58271752650789 30.05523055774854 21.357308527800797 28-29 21.60771985893042 26.842744292422545 30.115178056540294 21.434357792106745 30-31 21.879351849793597 26.409852228343233 30.109177900023663 21.601618021839503 32-33 21.82498846044712 26.506810624495756 29.93533273794035 21.732868177116774 34-35 22.29745632949379 26.43178718580053 29.844421982815543 21.426334501890132 36-37 22.19425415260454 26.28667649108676 29.915195727815618 21.603873628493083 38-39 21.976252050069252 26.70841828637257 29.921383909935233 21.393945753622948 40-41 22.17807262661145 26.548130723193257 29.80163083630049 21.472165813894797 42-43 21.880740918743165 26.668562937388735 29.523561981079794 21.927134162788303 44-45 22.22233482094406 26.617662250036283 29.21236177054008 21.947641158479577 46-47 22.318149068794042 26.956297457637092 28.891902175973932 21.833651297594937 48-49 22.377383260914193 27.10431755803447 28.61004941413069 21.908249766920644 50-51 22.229559300225823 27.258417989411072 28.59713688328772 21.914885827075388 52-53 22.473687858036694 27.451157943462075 28.14836922907941 21.92678496942182 54-55 22.177582277338935 27.21108293963735 28.330288809183525 22.281045973840193 56-57 22.381240675191336 27.451942502298106 28.14336766649973 22.023449156010834 58-59 22.679111513270158 27.098596816521763 28.14836922907941 22.073922441128666 60-61 22.47221681021914 27.134588453124568 28.700437130031474 21.692757606624816 62-63 22.39327057734377 27.197287780103853 28.60377294344246 21.80566869910992 64-65 22.23250139586093 27.254952854551945 28.482852812839564 22.02969293674756 66-67 22.179837883992516 27.384862721817665 28.232513164243468 22.20278622994635 68-69 22.168494470821603 27.27466489530716 28.424207039846433 22.1326335940248 70-71 22.280915214034188 27.21984384663967 28.30792888235671 22.19131205696943 72-73 22.30508767123825 27.152095329191294 28.343939050199374 22.198877949371077 74-75 22.085919653329604 27.615359569852544 28.089004277153258 22.2097164996646 76-77 22.291474068369073 27.21703251081057 28.225680964379723 22.26581245644064 78-79 22.152018735265006 27.332035760191747 28.19956169313027 22.316383811412976 80-81 22.25067700889559 27.5535755615153 28.05922373133567 22.13652369825344 82-83 22.190465698541274 27.42070303778409 28.21722769797966 22.171603565694976 84-85 22.15819991023219 27.361463849496058 28.011479557228284 22.468856683043462 86-87 22.15211680511951 27.306799117632828 28.075209117619753 22.46587495962791 88-89 22.13897544461603 27.285452579302554 27.97383757801457 22.601734398066846 90-91 22.19904633778343 27.125560076306055 28.074122145265935 22.60127144064458 92-93 22.320452825501018 27.052400854523434 28.020065282452034 22.607081037523518 94-95 22.040447930791448 26.921744178900337 28.18772793068684 22.85007995962137 96-97 21.857547652142305 27.04037601289815 28.279455934599174 22.822620400360375 98-99 21.832274177519746 27.061300277211153 27.955600449814323 23.15082509545478 100-101 21.475333085731325 27.18137152332777 28.261938514492563 23.081356876448343 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 355.0 1 261.0 2 229.5 3 307.5 4 276.5 5 255.0 6 493.0 7 1114.0 8 1884.0 9 2284.5 10 2124.0 11 1919.0 12 1973.0 13 2162.5 14 2296.0 15 2362.0 16 2529.5 17 2613.0 18 2668.5 19 2926.0 20 3194.0 21 3549.5 22 4024.5 23 4599.5 24 5461.5 25 6586.0 26 8031.5 27 9898.5 28 11868.5 29 14314.5 30 17271.0 31 20095.0 32 23143.5 33 26359.5 34 29884.0 35 32818.5 36 36484.0 37 40741.5 38 43313.5 39 44593.5 40 46093.0 41 48614.0 42 51175.0 43 54045.0 44 56809.0 45 58425.0 46 58798.5 47 61326.0 48 67957.5 49 66825.5 50 59831.0 51 55924.0 52 52100.0 53 49860.0 54 47604.0 55 43331.0 56 39344.5 57 35643.0 58 31815.0 59 28014.0 60 23663.0 61 18996.5 62 14716.5 63 11040.0 64 8164.0 65 6332.5 66 4733.0 67 3469.0 68 2433.0 69 1546.0 70 1018.5 71 743.5 72 565.5 73 439.5 74 341.0 75 269.0 76 180.5 77 101.0 78 59.5 79 36.5 80 17.5 81 10.0 82 8.0 83 4.0 84 1.5 85 2.0 86 1.0 87 1.0 88 1.5 89 1.0 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05727279503008129 2 0.0 3 0.0 4 0.0 5 0.017587193907639118 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0024190564110879087 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012095282055439543 24-25 2.2882966050831566E-4 26-27 0.0016018076235582099 28-29 0.004184313792152058 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.268995150118795E-5 40-41 0.0 42-43 0.013370190163985874 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.53799030023759E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6344975750593978E-4 84-85 0.001340288011548706 86-87 0.0 88-89 0.0 90-91 0.010101195013867076 92-93 9.480085935344506E-4 94-95 0.0 96-97 0.0 98-99 1.307598060047518E-4 100-101 0.01216066195844192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1529522.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.81905071475922 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1143119420409 45.16495363725385 2 12.618934727494102 14.59229653950436 3 3.934663242232643 6.824954806444445 4 1.765911105078778 4.084132149692255 5 0.9296220791059036 2.6874933068692077 6 0.5731472951115207 1.9883299513848058 7 0.3815854761994708 1.5444037000274928 8 0.2567504559495553 1.1876054106867915 9 0.2047315308281349 1.0653644487476 >10 1.1029260450216767 11.566897563002453 >50 0.08030877181104817 3.2241401464118433 >100 0.0343922624120638 3.603755282794819 >500 0.0018100444761205592 0.6998152599418952 >1k 6.787666785452096E-4 0.8335120906102974 >5k 2.262555595150699E-4 0.9323457066278882 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7986 0.522123905376974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6268 0.4098012320188922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4398 0.28754081340444926 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3188 0.20843113077157438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.53799030023759E-5 0.0 0.0 0.0 0.0 6 6.53799030023759E-5 0.0 0.0 0.0 0.0 7 6.53799030023759E-5 0.0 0.0 0.0 0.0 8 6.53799030023759E-5 0.0 0.0 0.0 0.0 9 6.53799030023759E-5 0.0 0.0 0.0 6.53799030023759E-5 10-11 1.307598060047518E-4 0.0 0.0 0.0 6.53799030023759E-5 12-13 1.307598060047518E-4 0.0 0.0 0.0 6.53799030023759E-5 14-15 1.307598060047518E-4 0.0 0.0 0.0 6.53799030023759E-5 16-17 1.307598060047518E-4 0.0 0.0 0.0 1.307598060047518E-4 18-19 1.6344975750593976E-4 0.0 0.0 0.0 1.307598060047518E-4 20-21 1.961397090071277E-4 0.0 0.0 0.0 1.307598060047518E-4 22-23 1.961397090071277E-4 0.0 0.0 0.0 1.307598060047518E-4 24-25 2.615196120095036E-4 0.0 0.0 1.307598060047518E-4 1.307598060047518E-4 26-27 2.615196120095036E-4 0.0 0.0 2.615196120095036E-4 1.307598060047518E-4 28-29 3.268995150118795E-4 0.0 0.0 6.864889815249469E-4 1.307598060047518E-4 30-31 3.268995150118795E-4 0.0 0.0 0.0015037377690546457 1.6344975750593976E-4 32-33 3.268995150118795E-4 0.0 0.0 0.002974785586608104 1.961397090071277E-4 34-35 3.268995150118795E-4 0.0 0.0 0.004347763549657997 2.615196120095036E-4 36-37 3.268995150118795E-4 0.0 0.0 0.006014951076218583 2.615196120095036E-4 38-39 3.922794180142554E-4 0.0 0.0 0.00957815578984807 3.268995150118795E-4 40-41 3.922794180142554E-4 0.0 0.0 0.01340288011548706 3.268995150118795E-4 42-43 3.922794180142554E-4 0.0 0.0 0.016704565217107045 3.268995150118795E-4 44-45 4.576593210166313E-4 0.0 0.0 0.018665962307178322 3.268995150118795E-4 46-47 4.576593210166313E-4 0.0 0.0 0.021150398621268607 3.268995150118795E-4 48-49 4.576593210166313E-4 0.0 0.0 0.02520395260741591 3.268995150118795E-4 50-51 4.576593210166313E-4 0.0 0.0 0.030401654896104795 3.268995150118795E-4 52-53 4.576593210166313E-4 0.0 0.0 0.04429488428410967 3.268995150118795E-4 54-55 4.576593210166313E-4 0.0 0.0 0.052107782692893595 3.268995150118795E-4 56-57 4.576593210166313E-4 0.0 0.0 0.07603682719176318 3.268995150118795E-4 58-59 4.576593210166313E-4 0.0 0.0 0.12127972006940732 3.268995150118795E-4 60-61 4.576593210166313E-4 0.0 0.0 0.16178256997937918 3.268995150118795E-4 62-63 4.576593210166313E-4 0.0 0.0 0.1853193350602345 3.268995150118795E-4 64-65 4.576593210166313E-4 0.0 0.0 0.22879697055681447 3.268995150118795E-4 66-67 4.576593210166313E-4 0.0 0.0 0.3129735956723735 3.268995150118795E-4 68-69 4.576593210166313E-4 0.0 0.0 0.42997093209512516 3.268995150118795E-4 70-71 4.576593210166313E-4 0.0 0.0 0.5465106091968601 3.922794180142554E-4 72-73 4.903492725178192E-4 0.0 0.0 0.6694575167928281 4.576593210166313E-4 74-75 5.230392240190072E-4 0.0 0.0 0.8179352765112238 4.576593210166313E-4 76-77 5.230392240190072E-4 0.0 0.0 0.9215624227699896 4.576593210166313E-4 78-79 5.230392240190072E-4 0.0 0.0 1.0612138955830646 4.576593210166313E-4 80-81 5.230392240190072E-4 0.0 0.0 1.2443430038927195 4.576593210166313E-4 82-83 5.884191270213832E-4 0.0 0.0 1.4121732148998183 4.576593210166313E-4 84-85 5.884191270213832E-4 0.0 0.0 1.616845001248756 4.576593210166313E-4 86-87 5.884191270213832E-4 0.0 0.0 1.8961806368264074 4.576593210166313E-4 88-89 5.884191270213832E-4 0.0 0.0 2.2604774563556456 4.576593210166313E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11145 0.0 25.75986 1 ACCGTCG 95 0.0012265928 25.000515 8 TACCGTG 155 1.4108919E-6 24.516636 7 GGTATCA 8120 0.0 23.707493 1 GTACATG 6835 0.0 22.67074 1 GAGTACT 6745 0.0 22.43004 12-13 ATCAACG 13100 0.0 21.759731 3 TCAACGC 13140 0.0 21.693493 4 CAACGCA 13240 0.0 21.637291 5 TACATGG 7025 0.0 21.573366 2 TATCAAC 13305 0.0 21.567293 2 AACGCAG 13475 0.0 21.290327 6 TGTACGG 180 5.1623574E-6 21.114998 5 GTACTTT 7140 0.0 21.054731 14-15 AGTACTT 6940 0.0 20.944237 12-13 TTCGGCG 115 0.0037198637 20.655975 4 GTACCGT 185 6.5569693E-6 20.539621 6 CCATGCG 140 4.8520707E-4 20.357563 9 ACATGGG 7150 0.0 20.199522 3 ACGGTAC 120 0.004763637 19.792076 8 >>END_MODULE