FastQCFastQC Report
Thu 26 May 2016
SRR937244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591122
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61170.3844456930392516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.2774143026116162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.1973450181695684No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27910.1754108107360718No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22230.13971273101622628No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17360.10910539858037284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100800.027.059031
GTACATG77250.026.573341
TACATGG78900.025.5293792
ACATGGG78650.024.040073
TCAACGC118050.023.0999454
ATCAACG118550.022.9617213
GGTATCA72100.022.8694171
CAACGCA119550.022.810115
TATCAAC119750.022.6919542
GAGTACT51400.022.55092812-13
AACGCAG121250.022.4888826
CATGGGG32400.022.1409874
AGTACTT54450.020.98238812-13
TCGTATG2056.8218105E-720.857099
GTACTTT55750.020.74808914-15
GTACCGT1656.319721E-520.153566
ACGCAGA133950.020.0735917
CGCAGAG134800.019.9470148
AGAGTAC97900.019.8701910-11
CATGGGA48150.019.1412584
Produced by FastQC (version 0.11.5)