FastQCFastQC Report
Thu 26 May 2016
SRR937244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591122
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81090.5096403669863153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.3968897419556766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44030.27672296656070366No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32670.20532680712101273No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.10131215582463192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110050.026.2187461
GTATAGG4000.026.1442361
GGTATCA79800.024.6610011
GTACATG71500.024.1996231
TACATGG74750.022.8108522
GAGTACT69700.021.91094212-13
ACATGGG74800.021.8431433
ATCAACG131800.021.7300223
TCAACGC132100.021.7166274
TATCAAC132450.021.6951032
CAACGCA132600.021.59965
AACGCAG136300.021.1526496
GTACTTT73400.020.7067614-15
AGTACTT72850.020.21366112-13
AGAGTAC115200.019.19524810-11
ATTCGCG2001.8189894E-1218.9996580-81
TGTACCG1759.943639E-518.999055
ACGCAGA151300.018.8067787
CGCAGAG152800.018.5599788
GTGTACG1801.226758E-418.4858231
Produced by FastQC (version 0.11.5)