##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937244_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1591122 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24449287986716 33.0 31.0 34.0 31.0 34.0 2 32.45414619369225 34.0 31.0 34.0 31.0 34.0 3 32.51347602509424 34.0 31.0 34.0 31.0 34.0 4 36.13983779999271 37.0 35.0 37.0 35.0 37.0 5 36.067812524746685 37.0 35.0 37.0 35.0 37.0 6 36.10912111076335 37.0 35.0 37.0 35.0 37.0 7 36.05832425169157 37.0 35.0 37.0 35.0 37.0 8 36.035801780127485 37.0 35.0 37.0 35.0 37.0 9 37.7538164892447 39.0 37.0 39.0 35.0 39.0 10-11 37.67933320009402 39.0 37.0 39.0 35.0 39.0 12-13 37.584056407993856 39.0 37.0 39.0 35.0 39.0 14-15 38.80233508178505 40.0 38.0 41.0 35.0 41.0 16-17 38.78058313567407 40.0 38.0 41.0 35.0 41.0 18-19 38.77463733139257 40.0 38.0 41.0 35.0 41.0 20-21 38.688307684765846 40.0 38.0 41.0 34.0 41.0 22-23 38.54248951368908 40.0 38.0 41.0 34.0 41.0 24-25 38.49079863140601 40.0 38.0 41.0 34.0 41.0 26-27 38.44748737054732 40.0 38.0 41.0 34.0 41.0 28-29 38.33779402208002 40.0 38.0 41.0 34.0 41.0 30-31 38.39842136555211 40.0 38.0 41.0 34.0 41.0 32-33 38.349664262074185 40.0 38.0 41.0 34.0 41.0 34-35 38.22771101147492 40.0 38.0 41.0 34.0 41.0 36-37 38.06968478847003 40.0 37.5 41.0 33.5 41.0 38-39 37.95209135440274 40.0 37.0 41.0 33.0 41.0 40-41 37.78736514233353 40.0 37.0 41.0 33.0 41.0 42-43 37.58430937413976 40.0 37.0 41.0 33.0 41.0 44-45 37.31101229195498 40.0 37.0 41.0 32.5 41.0 46-47 36.97104024707093 40.0 36.5 41.0 31.5 41.0 48-49 36.65755988541419 40.0 36.0 41.0 31.0 41.0 50-51 36.42257256200342 39.0 36.0 41.0 30.0 41.0 52-53 36.19122857958095 39.0 35.5 41.0 30.0 41.0 54-55 35.968646967360144 39.0 35.0 40.0 29.5 41.0 56-57 35.70704163477093 39.0 35.0 40.0 28.5 41.0 58-59 35.45018358114588 38.0 35.0 40.0 28.0 41.0 60-61 35.12267569677247 38.0 34.0 40.0 28.0 41.0 62-63 34.76763975358269 37.5 34.0 40.0 27.0 41.0 64-65 34.37587909663746 37.0 34.0 40.0 26.0 41.0 66-67 33.96839494394521 36.5 33.5 39.0 26.0 41.0 68-69 33.539657235585956 36.0 33.0 39.0 26.0 40.0 70-71 33.133293047296185 35.5 32.5 38.5 25.0 40.0 72-73 32.66498797703758 35.0 32.0 38.0 24.0 40.0 74-75 32.201809477840165 35.0 32.0 37.0 22.5 39.0 76-77 30.923678699684878 34.0 30.5 36.0 21.5 38.5 78-79 31.527228898852506 34.5 31.0 36.0 22.0 38.5 80-81 31.339224144974423 34.5 31.0 36.0 21.5 37.5 82-83 31.008324628783967 34.0 31.0 35.0 20.0 37.0 84-85 30.658688020151818 34.0 31.0 35.0 19.0 36.5 86-87 30.263967502177707 34.0 31.0 35.0 18.0 36.0 88-89 29.8223568023068 34.0 30.0 35.0 12.5 36.0 90-91 29.32590367049164 34.0 29.0 35.0 7.0 35.0 92-93 28.995751425723483 34.0 29.0 35.0 2.0 35.0 94-95 28.580136846828843 34.0 29.0 35.0 2.0 35.0 96-97 28.13201281862736 34.0 29.0 35.0 2.0 35.0 98-99 27.421690165807526 33.0 27.5 35.0 2.0 35.0 100-101 24.93873631311741 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.022372408009786682 1101 2 0.032175690311106564 1101 3 0.058663146871943184 1101 4 0.003872498711928074 1101 5 0.09510942276332202 1101 6 -0.01416253590848271 1101 7 0.0684727831604377 1101 8 -0.014624207749911022 1101 9 -9.413056221063698E-5 1101 10-11 -0.005063507038961745 1101 12-13 -0.09519658224490257 1101 14-15 -0.07199148766510888 1101 16-17 -0.18128209966111797 1101 18-19 -0.10175354487235211 1101 20-21 -0.012758150788371836 1101 22-23 0.03011025686146951 1101 24-25 0.0016540022838995583 1101 26-27 -0.12626245791111046 1101 28-29 -0.19688057907806922 1101 30-31 -0.05811264394696991 1101 32-33 0.0168402110835828 1101 34-35 -0.005854386303767001 1101 36-37 -0.021083893021916822 1101 38-39 0.017570489744677786 1101 40-41 0.004512198506787968 1101 42-43 0.07458379768162615 1101 44-45 -0.09652534432425597 1101 46-47 -0.09775813705471847 1101 48-49 0.13536133287314556 1101 50-51 0.22680735578073552 1101 52-53 0.1442212722399674 1101 54-55 0.10487190465060792 1101 56-57 0.197166382311309 1101 58-59 0.3033270662729777 1101 60-61 0.21880128568099622 1101 62-63 0.1644175006645412 1101 64-65 0.04693336242920054 1101 66-67 0.0029997250238054107 1101 68-69 0.19590809527938546 1101 70-71 0.16736180005282364 1101 72-73 0.14794753715310094 1101 74-75 0.10170545349988913 1101 76-77 0.06577141164133238 1101 78-79 -0.13731957958542296 1101 80-81 0.025818737881479592 1101 82-83 0.08670526680926471 1101 84-85 0.038234887170727205 1101 86-87 0.092143487757955 1101 88-89 0.1656935393883252 1101 90-91 -0.03986751982025183 1101 92-93 0.12141855986814676 1101 94-95 0.22443142180910058 1101 96-97 0.1740917095100194 1101 98-99 0.21617602245833467 1101 100-101 0.9455187158418674 1102 1 0.022479157798962035 1102 2 -0.004945125541951256 1102 3 0.011255209669286614 1102 4 0.010257559434769803 1102 5 -0.033453262492038505 1102 6 -0.048048477317081506 1102 7 -0.04979459659482188 1102 8 -0.03340042816687827 1102 9 -0.0777371663838835 1102 10-11 -0.05521402424169253 1102 12-13 -0.04743405782588894 1102 14-15 -0.06682141745933023 1102 16-17 -0.11131472951333876 1102 18-19 -0.08080603374368422 1102 20-21 0.020165263354549268 1102 22-23 0.09909108104100284 1102 24-25 -0.22056738966218603 1102 26-27 -0.14877581053057298 1102 28-29 -0.13571917695517044 1102 30-31 -0.13354026581075118 1102 32-33 -0.051444426554255074 1102 34-35 -0.14226816271988696 1102 36-37 -0.13309520138292186 1102 38-39 -0.19118975378717096 1102 40-41 -0.0985666433291641 1102 42-43 -0.1039144818676121 1102 44-45 -0.17516026360362247 1102 46-47 -0.21622442725045943 1102 48-49 -0.11259476449689743 1102 50-51 -0.15236522660341478 1102 52-53 -0.166733746957199 1102 54-55 -0.15547339533408433 1102 56-57 -0.11902684904038807 1102 58-59 -0.019368472653333413 1102 60-61 -0.16476084803379365 1102 62-63 -0.17014096441582893 1102 64-65 -0.21131483794727046 1102 66-67 -0.17596042527988942 1102 68-69 -0.04730350771103531 1102 70-71 -0.1765118295467616 1102 72-73 -0.15744884260571013 1102 74-75 -0.1417242748930576 1102 76-77 -0.1918667136900396 1102 78-79 -0.13302058765926716 1102 80-81 -0.1967958491213757 1102 82-83 -0.22306180515077045 1102 84-85 -0.09986731507742164 1102 86-87 -0.12765165939662992 1102 88-89 0.027937918122340477 1102 90-91 -0.06291978095870476 1102 92-93 -0.1561910276862548 1102 94-95 0.041357668379767176 1102 96-97 0.06633011025584423 1102 98-99 0.3937998433179537 1102 100-101 1.0893930571067116 1103 1 0.029558803816655654 1103 2 0.11776873876483762 1103 3 -0.026502902425107777 1103 4 -0.006851585108002212 1103 5 -0.002184825913872146 1103 6 -0.017370011240380734 1103 7 -0.051564508099247064 1103 8 -0.022780959140327184 1103 9 -0.041748965793914294 1103 10-11 -0.025715499167944245 1103 12-13 -0.06542815812087355 1103 14-15 -0.05236714017319599 1103 16-17 -0.13196369706495403 1103 18-19 -0.09083553226875551 1103 20-21 -0.08543945640946049 1103 22-23 -0.07023045288229213 1103 24-25 -0.08988892358549094 1103 26-27 -0.07591445359842197 1103 28-29 -0.13276932444779277 1103 30-31 -0.18309778793464915 1103 32-33 -0.08359781888463402 1103 34-35 -0.007459903132868817 1103 36-37 -0.06996835772510224 1103 38-39 -0.002694178565931793 1103 40-41 -0.026590242149225674 1103 42-43 0.005525046155987923 1103 44-45 0.02631466265005855 1103 46-47 -0.15103268683748183 1103 48-49 -0.33383370254999534 1103 50-51 -0.19425313220813223 1103 52-53 -0.1764682602315375 1103 54-55 -0.2242049587559194 1103 56-57 -0.30575251275720206 1103 58-59 -0.33854251395126767 1103 60-61 -0.38688474183910415 1103 62-63 -0.2742707579261463 1103 64-65 -0.5378635105136453 1103 66-67 -0.13377753442443918 1103 68-69 -0.18860144281427438 1103 70-71 -0.09450592984174477 1103 72-73 -0.058628783608661195 1103 74-75 0.17685979590340395 1103 76-77 0.2057734043040611 1103 78-79 -0.05661940771826224 1103 80-81 -0.1141999789148862 1103 82-83 -0.11863702638970608 1103 84-85 -0.1948525658148874 1103 86-87 -0.10906758860016552 1103 88-89 0.02616800661791885 1103 90-91 0.0798530803982267 1103 92-93 -0.0019137415505596778 1103 94-95 0.008024335046435738 1103 96-97 0.04774603945938338 1103 98-99 0.20412432709376915 1103 100-101 0.8197765379326682 1104 1 0.02982480207743521 1104 2 -0.0064780257755501225 1104 3 0.007473260038445062 1104 4 0.009031580271297912 1104 5 -0.01732285513957521 1104 6 0.01954406791114849 1104 7 -0.038859680912857186 1104 8 -0.07335643614682397 1104 9 0.023190417294735255 1104 10-11 -0.018449995391108587 1104 12-13 -0.03832361739415546 1104 14-15 -0.019969063545239862 1104 16-17 -0.08914309243045437 1104 18-19 -0.017728638480505765 1104 20-21 -0.16277418793877985 1104 22-23 -0.12654620645877657 1104 24-25 -0.03611976233257508 1104 26-27 -0.1752162081638673 1104 28-29 -0.08815323155302224 1104 30-31 -0.20294449791128955 1104 32-33 -0.019169118354348313 1104 34-35 -0.019741861289304552 1104 36-37 0.027082642326050177 1104 38-39 0.00613321243445597 1104 40-41 -0.03589932871801693 1104 42-43 0.04057457980648138 1104 44-45 0.17071250056163478 1104 46-47 0.12080730497795855 1104 48-49 0.24978779556585096 1104 50-51 0.2764367581528404 1104 52-53 0.43610612550003935 1104 54-55 0.40633198260510994 1104 56-57 0.41847208526144897 1104 58-59 0.20507267523147021 1104 60-61 0.3953061630665218 1104 62-63 0.418861883129658 1104 64-65 0.42864543282531997 1104 66-67 0.44533222396047023 1104 68-69 0.4990475417885136 1104 70-71 0.44847666091310856 1104 72-73 0.5281838410780253 1104 74-75 0.5079909705585379 1104 76-77 0.562917844769899 1104 78-79 0.4215329274395465 1104 80-81 0.38624079384416277 1104 82-83 0.41475598604059627 1104 84-85 0.3990379997724425 1104 86-87 0.4418966550951602 1104 88-89 0.40121617617475636 1104 90-91 0.35803440254599295 1104 92-93 0.515404279831607 1104 94-95 0.5862448748864928 1104 96-97 0.5597935440205717 1104 98-99 0.7496194078310587 1104 100-101 1.0775672177628373 1105 1 0.04376976470535965 1105 2 0.10366719427136672 1105 3 0.04242334788516189 1105 4 0.07917097695793984 1105 5 -0.017211681897919107 1105 6 -0.008018624201831415 1105 7 -0.027540855246400042 1105 8 -0.003168070294215397 1105 9 0.06893787536488816 1105 10-11 0.08042735339176943 1105 12-13 0.05569089468435351 1105 14-15 0.1197570069108096 1105 16-17 0.13732474177583498 1105 18-19 0.10653459063894388 1105 20-21 0.05501303441763383 1105 22-23 0.06577142475708087 1105 24-25 -0.023929748733699796 1105 26-27 0.002788860806553828 1105 28-29 -0.0034756174101886472 1105 30-31 -0.05611128093487139 1105 32-33 0.07456729518364824 1105 34-35 0.1111616529100985 1105 36-37 0.09994264014229515 1105 38-39 0.05218540430094265 1105 40-41 0.15340901489994252 1105 42-43 0.21101292001645078 1105 44-45 0.1458752072330185 1105 46-47 0.22794720789989498 1105 48-49 0.3531413562528769 1105 50-51 0.2489599275166725 1105 52-53 0.3665310322766473 1105 54-55 0.41437715335199243 1105 56-57 0.5102462579968687 1105 58-59 0.38325194870110124 1105 60-61 0.3908140692707178 1105 62-63 0.34045182592176104 1105 64-65 0.36397056492626234 1105 66-67 0.43488243555359674 1105 68-69 0.5384228871369388 1105 70-71 0.34999834465832436 1105 72-73 0.3850324105497194 1105 74-75 0.4407253690815267 1105 76-77 0.6003635402809486 1105 78-79 0.549866553043799 1105 80-81 0.3613278960937656 1105 82-83 0.4300674530972515 1105 84-85 0.501122498053558 1105 86-87 0.4606640879528108 1105 88-89 0.5294116624087941 1105 90-91 0.7411694879607928 1105 92-93 0.8532245232877713 1105 94-95 0.9849925215541084 1105 96-97 0.9982864517633274 1105 98-99 1.1054447533449228 1105 100-101 1.5848488996551673 1106 1 -0.09584650181609078 1106 2 0.044380988685361444 1106 3 0.011589161685478189 1106 4 0.030804666494248067 1106 5 -0.030538616799063334 1106 6 1.3871355513117578E-4 1106 7 -0.001748916607674289 1106 8 -0.07981394758360949 1106 9 -0.13609263215636247 1106 10-11 -0.17884993109061043 1106 12-13 -0.15583344666293897 1106 14-15 -0.09664774190208902 1106 16-17 -0.015394105498188537 1106 18-19 -0.12489077620413269 1106 20-21 9.707132326965962E-4 1106 22-23 -0.022750132807921375 1106 24-25 0.017363851087480953 1106 26-27 -0.08363756396665423 1106 28-29 -0.08557082979547204 1106 30-31 -0.05798404941531743 1106 32-33 0.006168961606000778 1106 34-35 0.028797646677929833 1106 36-37 -0.04169745666992242 1106 38-39 -0.004135777980749822 1106 40-41 0.07498654459660514 1106 42-43 -0.09070560291410601 1106 44-45 0.1260176265169335 1106 46-47 0.08680506785647424 1106 48-49 -0.07262494664944086 1106 50-51 0.18762236555811285 1106 52-53 0.18602742437009567 1106 54-55 0.19538113899596965 1106 56-57 0.1776801174652789 1106 58-59 0.09992930521559629 1106 60-61 0.07112549206403429 1106 62-63 0.15652221575077618 1106 64-65 0.11936003908523674 1106 66-67 0.16583604515150796 1106 68-69 0.0920659143161231 1106 70-71 0.09085504254995413 1106 72-73 0.17199960675628745 1106 74-75 0.1912624593160004 1106 76-77 0.17583889581511514 1106 78-79 0.11835675077393049 1106 80-81 0.05473957816287722 1106 82-83 -0.09678026001597573 1106 84-85 -0.13042033551446863 1106 86-87 0.015335860984013294 1106 88-89 0.12504788301807324 1106 90-91 -0.014719601164681961 1106 92-93 0.11879914516894985 1106 94-95 0.2325727136529956 1106 96-97 0.20532934801122238 1106 98-99 0.06888845852798298 1106 100-101 0.8324617582487086 1107 1 -0.055296181168024816 1107 2 -0.0739015029767387 1107 3 -0.039958819257222444 1107 4 -0.025006646915230135 1107 5 0.01926928176406051 1107 6 -0.09971745531124299 1107 7 -0.05440927003251517 1107 8 -0.05779707191980776 1107 9 0.09091757449801463 1107 10-11 0.02205348780547922 1107 12-13 0.08796816304389665 1107 14-15 -0.02796151716235329 1107 16-17 0.1345331951141091 1107 18-19 0.06377099332378577 1107 20-21 0.03826262511555001 1107 22-23 0.0906622183804231 1107 24-25 0.12146767600279418 1107 26-27 -0.01073601578975314 1107 28-29 0.11025179676221342 1107 30-31 0.16981814293171738 1107 32-33 0.10959706759426524 1107 34-35 0.12142437418651753 1107 36-37 0.07379006032919477 1107 38-39 0.03824541763350453 1107 40-41 0.16941116127552647 1107 42-43 0.11384715541692714 1107 44-45 0.12960589324072913 1107 46-47 -0.0282533072044302 1107 48-49 0.13836069505819637 1107 50-51 0.1717186396655066 1107 52-53 0.10436667772844288 1107 54-55 0.15498627615809824 1107 56-57 0.19083753090858835 1107 58-59 0.15279083933597093 1107 60-61 0.10804247628702512 1107 62-63 0.12138777087232455 1107 64-65 0.2023728767650752 1107 66-67 0.11501342512566737 1107 68-69 0.22365474539097363 1107 70-71 0.2634733047929174 1107 72-73 0.300175877623893 1107 74-75 0.33009216246996687 1107 76-77 0.4361314438992636 1107 78-79 0.39030569529377956 1107 80-81 0.35573463878489164 1107 82-83 0.181682236113911 1107 84-85 0.22840437731315077 1107 86-87 0.39831336140531803 1107 88-89 0.6344919449869515 1107 90-91 0.5198762934427599 1107 92-93 0.6182481844004926 1107 94-95 0.4979244990010301 1107 96-97 0.6913365121341712 1107 98-99 0.8887726228638719 1107 100-101 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2316 72-73 -0.4546406349984693 2316 74-75 -0.444471406258959 2316 76-77 -0.5702249323429847 2316 78-79 -0.5194005858399713 2316 80-81 -0.5381652564219372 2316 82-83 -0.4977723427516416 2316 84-85 -0.5410119098300648 2316 86-87 -0.7916140780157868 2316 88-89 -0.5428481460986774 2316 90-91 -0.47746762184860003 2316 92-93 -0.16082372556677882 2316 94-95 -0.16038495374022688 2316 96-97 -0.312143763407299 2316 98-99 -0.3186103291249651 2316 100-101 -0.7177970459432039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 17.0 5 31.0 6 69.0 7 127.0 8 321.0 9 605.0 10 1060.0 11 1794.0 12 2808.0 13 4130.0 14 5349.0 15 7502.0 16 10409.0 17 14699.0 18 13426.0 19 7918.0 20 6869.0 21 7095.0 22 7285.0 23 8097.0 24 9384.0 25 11124.0 26 13447.0 27 16943.0 28 21556.0 29 27120.0 30 34408.0 31 43343.0 32 56379.0 33 74312.0 34 103507.0 35 149918.0 36 232348.0 37 362746.0 38 305516.0 39 29447.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.994466617945065 19.838350777931378 15.234222188119894 21.932960416003663 2 15.783893378383304 20.975387179612877 39.416147850384824 23.824571591618994 3 19.696729729084257 24.842092561098394 29.85465602260543 25.60652168721192 4 13.032941534338663 16.94552649011201 37.43622424930332 32.58530772624601 5 13.66739447144906 37.80097367458453 33.89552796014797 14.636103893818436 6 31.820815751400584 36.69379217935519 17.121188695775686 14.364203373468534 7 28.764230524120716 32.99344739121199 20.312458755519692 17.929863329147608 8 26.805612643153697 34.72027914892762 19.41937827520454 19.05472993271415 9 26.50978366209505 16.922209610576687 20.21837420386369 36.34963252346458 10-11 25.037780003324706 27.108460426762417 26.936600299348978 20.9171592705639 12-13 26.244253736878033 24.127894187050686 27.879008242438562 21.74884383363272 14-15 22.862231808748795 25.47441365275573 26.587810362750307 25.07554417574517 16-17 21.93650141221101 29.09965420627708 26.905416429412703 22.058427952099212 18-19 21.84672199869023 27.406917885617823 29.65599118106594 21.090368934626007 20-21 22.67796645322183 26.163558219783972 29.874368487887317 21.28410683910688 22-23 22.271276027427394 26.188038539133057 29.889699643645002 21.650985789794547 24-25 22.27935215038558 26.30361822878368 29.94239870279239 21.47463091803835 26-27 22.05653462182302 26.578870709097746 29.92398091022419 21.44061375885504 28-29 21.70988313280386 26.80830844718947 30.009374138752964 21.4724342812537 30-31 21.898270805437566 26.42270380578669 29.997237788684867 21.68178760009088 32-33 21.798171353296606 26.58759039218866 29.834073062907812 21.78016519160693 34-35 22.403876006993805 26.428645949210683 29.694108936963982 21.47336910683153 36-37 22.265207276754165 26.26832708878612 29.771296535179747 21.695169099279973 38-39 22.09243203665354 26.602853973471436 29.814626849724878 21.490087140150145 40-41 22.241401578321693 26.54453261082363 29.676884937375892 21.537180873478786 42-43 21.957925959821704 26.645488324936274 29.415151544674874 21.981434170567155 44-45 22.231893958938972 26.631783470694693 29.161700299694647 21.974622270671688 46-47 22.36255296576881 26.961666044464224 28.775009081641763 21.90077190812521 48-49 22.48165131272146 27.0656178470287 28.483422389986433 21.9693084502634 50-51 22.28587751284942 27.198134398242246 28.506550723326058 22.009437365582272 52-53 22.61630472081965 27.353716434063507 28.08078198906181 21.949196856055035 54-55 22.265985889202714 27.259411911845856 28.177600460555507 22.297001738395924 56-57 22.42395617683622 27.431491739791163 28.136120297500756 22.008431785871856 58-59 22.767738740335435 27.08695499150914 28.066766721847852 22.078539546307574 60-61 22.55509005594794 27.07875323199604 28.62624613323177 21.73991057882425 62-63 22.39140053371143 27.214003703047286 28.509756008653014 21.884839754588274 64-65 22.264917460760394 27.244045396896027 28.433583345588836 22.057453796754743 66-67 22.223468722071598 27.33426475154011 28.183162573328758 22.25910395305954 68-69 22.205871077139278 27.280591934496538 28.341792772647228 22.17174421571696 70-71 22.34310128324541 27.19492911291529 28.229262118178237 22.232707485661063 72-73 22.369592023741735 27.101535897310203 28.269485306594973 22.259386772353093 74-75 22.20546256038192 27.571927231224258 27.968345607690676 22.254264600703152 76-77 22.35312565598364 27.248319110665303 28.107649821949543 22.290905411401514 78-79 22.237515413651497 27.325151685414443 28.137722940164235 22.29960996076982 80-81 22.283276292853817 27.533692430150687 27.96263516016847 22.22039611682703 82-83 22.275308801425922 27.396382968761145 28.135677480805082 22.192630749007847 84-85 22.199617500454085 27.342212652401184 27.89317917709176 22.564990670052975 86-87 22.19157299063177 27.282320274623817 27.9585412055141 22.567565529230315 88-89 22.23145051102304 27.21504070712365 27.89971479245463 22.653793989398675 90-91 22.2258921758544 27.17486131346488 27.969834028703993 22.629412481976722 92-93 22.328121872204136 27.04734813190943 27.969931049826958 22.654598946059476 94-95 22.099436749664704 26.93382405623202 28.04165865345335 22.92508054064993 96-97 21.947939881417014 26.99393886829545 28.191301484109953 22.866819766177578 98-99 21.918711347981297 27.031807783196744 27.841551862838752 23.207929005983207 100-101 21.661583794479323 27.05036066183773 28.15560251520462 23.13245302847833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 322.0 1 240.0 2 210.0 3 309.0 4 275.5 5 241.0 6 513.5 7 1118.5 8 1909.5 9 2330.0 10 2151.0 11 1919.5 12 1914.0 13 2064.0 14 2243.5 15 2377.0 16 2521.0 17 2686.5 18 2782.0 19 2934.5 20 3181.0 21 3491.5 22 3950.0 23 4619.0 24 5552.0 25 6611.5 26 8136.0 27 10136.0 28 12075.0 29 14637.0 30 17783.5 31 20875.0 32 24037.0 33 27050.0 34 30746.0 35 33814.0 36 37411.5 37 41811.0 38 44714.0 39 46323.0 40 47785.5 41 50351.0 42 53078.0 43 55968.5 44 59078.0 45 61430.0 46 61601.5 47 64041.5 48 71021.0 49 70047.0 50 62724.5 51 58364.5 52 54624.5 53 52137.5 54 49710.0 55 45538.5 56 41735.0 57 37431.5 58 33041.0 59 29363.0 60 24742.5 61 19795.5 62 15498.0 63 11711.0 64 8626.0 65 6653.5 66 4952.5 67 3705.0 68 2605.0 69 1618.0 70 1118.0 71 820.5 72 642.0 73 511.0 74 405.0 75 314.0 76 197.5 77 104.5 78 65.0 79 38.0 80 27.0 81 17.5 82 8.0 83 6.5 84 4.5 85 3.0 86 3.0 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07139615943969099 2 0.0 3 0.0 4 0.0 5 0.0022625543484409115 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.571218297528411E-4 12-13 0.005530688407300006 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.142436595056821E-5 22-23 7.541847828136372E-4 24-25 7.541847828136372E-4 26-27 5.342142211596597E-4 28-29 0.002671071105798298 30-31 3.456680254562504E-4 32-33 0.0 34-35 0.0 36-37 3.142436595056821E-5 38-39 4.39941123307955E-4 40-41 9.427309785170465E-5 42-43 0.011846985963364217 44-45 1.571218297528411E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.142436595056821E-5 82-83 6.599116849619326E-4 84-85 5.656385871102279E-4 86-87 0.0 88-89 0.0 90-91 0.007793242755740918 92-93 0.0028596173015017078 94-95 0.0 96-97 0.0 98-99 1.2569746380227285E-4 100-101 0.009930099640379556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1591122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.086770095190516 #Duplication Level Percentage of deduplicated Percentage of total 1 77.63056112057471 44.31677995050885 2 12.87353505353454 14.69817071827005 3 4.052471319132275 6.94027495537973 4 1.749990985913262 3.996053323259448 5 0.9480208652215242 2.7059724589172376 6 0.5922130906926215 2.028451953343914 7 0.3840237191873824 1.5345871637845074 8 0.28206976824619084 1.2881961608539176 9 0.21715134177077666 1.1156821851177425 >10 1.148501700982359 11.926824102573116 >50 0.08233176251118193 3.2403507472615627 >100 0.036373836839414156 3.760456094759478 >500 0.0017634786519914207 0.6500220026024406 >1k 7.715219102462466E-4 0.8906264104963263 >5k 2.2043483149892758E-4 0.9075517728717067 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8109 0.5096403669863153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6315 0.3968897419556766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4403 0.27672296656070366 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3267 0.20532680712101273 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.10131215582463192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.284873190113644E-5 0.0 0.0 0.0 0.0 7 6.284873190113644E-5 0.0 0.0 0.0 0.0 8 1.8854619570340928E-4 0.0 0.0 0.0 0.0 9 1.8854619570340928E-4 0.0 0.0 0.0 0.0 10-11 3.1424365950568213E-4 0.0 0.0 0.0 0.0 12-13 3.1424365950568213E-4 0.0 0.0 0.0 3.142436595056822E-5 14-15 3.7709239140681856E-4 0.0 0.0 0.0 1.2569746380227287E-4 16-17 3.7709239140681856E-4 0.0 0.0 3.142436595056822E-5 1.2569746380227287E-4 18-19 3.7709239140681856E-4 0.0 0.0 6.284873190113644E-5 1.2569746380227287E-4 20-21 3.7709239140681856E-4 0.0 0.0 9.427309785170465E-5 1.2569746380227287E-4 22-23 3.7709239140681856E-4 0.0 0.0 1.2569746380227287E-4 1.2569746380227287E-4 24-25 4.39941123307955E-4 0.0 0.0 1.8854619570340928E-4 1.2569746380227287E-4 26-27 5.027898552090915E-4 0.0 0.0 2.199705616539775E-4 1.2569746380227287E-4 28-29 5.970629530607961E-4 0.0 0.0 5.970629530607961E-4 1.2569746380227287E-4 30-31 6.284873190113643E-4 0.0 0.0 0.0016969157613306836 1.2569746380227287E-4 32-33 6.284873190113643E-4 0.0 0.0 0.0032995584248096625 1.2569746380227287E-4 34-35 6.284873190113643E-4 0.0 0.0 0.005122171649942619 1.2569746380227287E-4 36-37 6.284873190113643E-4 0.0 0.0 0.007133331070778985 1.2569746380227287E-4 38-39 7.541847828136371E-4 0.0 0.0 0.010872830618896602 1.2569746380227287E-4 40-41 7.541847828136371E-4 0.0 0.0 0.015020846924371608 1.2569746380227287E-4 42-43 7.541847828136371E-4 0.0 0.0 0.01904316576604434 1.2569746380227287E-4 44-45 7.541847828136371E-4 0.0 0.0 0.021274295748534684 1.2569746380227287E-4 46-47 7.541847828136371E-4 0.0 0.0 0.024322459245739798 1.2569746380227287E-4 48-49 7.541847828136371E-4 0.0 0.0 0.02891041667452276 1.2569746380227287E-4 50-51 7.541847828136371E-4 0.0 0.0 0.0351952898646364 1.2569746380227287E-4 52-53 7.541847828136371E-4 0.0 0.0 0.04965049820189778 1.2569746380227287E-4 54-55 7.541847828136371E-4 0.0 0.0 0.060177660795338137 1.2569746380227287E-4 56-57 7.541847828136371E-4 0.0 0.0 0.08732831297662907 1.2569746380227287E-4 58-59 7.541847828136371E-4 0.0 0.0 0.13490480302578936 1.2569746380227287E-4 60-61 7.541847828136371E-4 0.0 0.0 0.177359121425007 1.2569746380227287E-4 62-63 7.541847828136371E-4 0.0 0.0 0.2065209330271343 1.2569746380227287E-4 64-65 7.541847828136371E-4 0.0 0.0 0.253123267731827 1.2569746380227287E-4 66-67 7.541847828136371E-4 0.0 0.0 0.3416142822486271 1.2569746380227287E-4 68-69 7.541847828136371E-4 0.0 0.0 0.4636979439665846 1.2569746380227287E-4 70-71 7.541847828136371E-4 0.0 0.0 0.5777683923671473 2.5139492760454575E-4 72-73 7.856091487642054E-4 0.0 0.0 0.707111082619686 2.5139492760454575E-4 74-75 8.7988224661591E-4 0.0 0.0 0.8608390808498657 2.5139492760454575E-4 76-77 8.7988224661591E-4 0.0 0.0 0.9761350795225004 3.1424365950568213E-4 78-79 8.7988224661591E-4 0.0 0.0 1.1199644024782511 3.7709239140681856E-4 80-81 8.7988224661591E-4 0.0 0.0 1.3093904804282763 3.7709239140681856E-4 82-83 8.7988224661591E-4 0.0 0.0 1.4875666353679982 3.7709239140681856E-4 84-85 8.7988224661591E-4 0.0 0.0 1.703074937056995 3.7709239140681856E-4 86-87 8.7988224661591E-4 0.0 0.0 1.9965470906693517 3.7709239140681856E-4 88-89 8.7988224661591E-4 0.0 0.0 2.3668518190308476 3.7709239140681856E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11005 0.0 26.218746 1 GTATAGG 400 0.0 26.144236 1 GGTATCA 7980 0.0 24.661001 1 GTACATG 7150 0.0 24.199623 1 TACATGG 7475 0.0 22.810852 2 GAGTACT 6970 0.0 21.910942 12-13 ACATGGG 7480 0.0 21.843143 3 ATCAACG 13180 0.0 21.730022 3 TCAACGC 13210 0.0 21.716627 4 TATCAAC 13245 0.0 21.695103 2 CAACGCA 13260 0.0 21.5996 5 AACGCAG 13630 0.0 21.152649 6 GTACTTT 7340 0.0 20.70676 14-15 AGTACTT 7285 0.0 20.213661 12-13 AGAGTAC 11520 0.0 19.195248 10-11 ATTCGCG 200 1.8189894E-12 18.99965 80-81 TGTACCG 175 9.943639E-5 18.99905 5 ACGCAGA 15130 0.0 18.806778 7 CGCAGAG 15280 0.0 18.559978 8 GTGTACG 180 1.226758E-4 18.485823 1 >>END_MODULE