FastQCFastQC Report
Thu 26 May 2016
SRR937243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499352
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.19194958888906674No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23350.15573394373035818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.1331908717899466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.040.529021
GTATCAA40350.034.633621
ATCAACG47300.029.03163
TAGGCCG1153.8558555E-628.9224115
TCAACGC48200.028.3909384
CAACGCA49350.027.7293435
TATCAAC50550.027.5429082
AACGCAG50650.027.1060056
ACGCAGA57750.023.3629677
CGCAGAG58450.022.9206148
GTACATG44650.022.1429771
TATACCG1100.002872362721.5979045
ACATGGG45050.021.1999843
GTATAGG4050.021.1257231
TACATGG46150.021.1079222
GCAGAGT65150.020.1988669
TCGCGCT1652.7284841E-1120.14557660-61
GAGTACT30450.019.8886212-13
GTGTAGC6800.019.5723611
AGAGTAC54750.019.52295310-11
Produced by FastQC (version 0.11.5)