##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937243_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1499352 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82508577038614 33.0 31.0 34.0 30.0 34.0 2 31.94844239378078 33.0 31.0 34.0 30.0 34.0 3 32.00660618720621 34.0 31.0 34.0 30.0 34.0 4 35.61609682049312 37.0 35.0 37.0 33.0 37.0 5 35.61702922329113 37.0 35.0 37.0 33.0 37.0 6 35.64790656230158 37.0 35.0 37.0 33.0 37.0 7 35.5851494512296 37.0 35.0 37.0 33.0 37.0 8 35.56560234021097 37.0 35.0 37.0 33.0 37.0 9 37.19783413101126 39.0 37.0 39.0 34.0 39.0 10-11 37.08684118205731 39.0 37.0 39.0 33.0 39.0 12-13 36.94716750969752 39.0 37.0 39.0 33.0 39.0 14-15 38.123729784600286 40.0 38.0 41.0 33.0 41.0 16-17 38.04053251004434 40.0 38.0 41.0 33.0 41.0 18-19 37.93760371147002 40.0 38.0 41.0 33.0 41.0 20-21 37.79124348385169 40.0 38.0 41.0 33.0 41.0 22-23 37.86997582955837 40.0 38.0 41.0 33.0 41.0 24-25 37.8519293668198 40.0 38.0 41.0 33.0 41.0 26-27 37.78227260843351 40.0 38.0 41.0 33.0 41.0 28-29 37.65611544187089 40.0 37.0 41.0 32.5 41.0 30-31 37.438822904828214 40.0 37.0 41.0 32.0 41.0 32-33 37.2397892556251 39.5 37.0 41.0 31.5 41.0 34-35 37.301234466622915 40.0 37.0 41.0 32.0 41.0 36-37 37.200596324278756 40.0 37.0 41.0 31.0 41.0 38-39 37.078840392382844 40.0 37.0 41.0 31.0 41.0 40-41 36.885774321173415 39.5 36.0 41.0 30.5 41.0 42-43 36.67218805190509 39.0 36.0 41.0 30.0 41.0 44-45 36.49352486941025 39.0 36.0 41.0 30.0 41.0 46-47 36.20027218425026 39.0 35.5 40.5 29.5 41.0 48-49 35.945279027206425 39.0 35.0 40.0 29.0 41.0 50-51 35.00523326076865 38.0 34.0 39.5 27.0 40.5 52-53 35.11865826036848 38.0 34.5 39.5 28.0 40.5 54-55 35.43730524920099 38.0 35.0 40.0 28.0 41.0 56-57 35.192409787694956 38.0 34.5 40.0 27.0 41.0 58-59 34.93138102326872 38.0 34.0 40.0 26.0 41.0 60-61 34.60572967521969 38.0 34.0 40.0 26.0 41.0 62-63 34.229646874116284 37.5 34.0 40.0 25.5 41.0 64-65 33.802011802431984 37.0 33.0 39.5 24.0 41.0 66-67 33.3672569883523 36.0 32.5 39.0 22.5 40.5 68-69 32.878388463816364 36.0 32.0 39.0 22.0 40.0 70-71 32.40759441412024 35.0 31.5 38.5 20.5 40.0 72-73 31.909207444282597 35.0 31.0 38.0 19.0 40.0 74-75 31.355732009561464 35.0 30.5 37.0 17.5 39.0 76-77 30.820848606598048 34.5 30.0 36.5 14.0 39.0 78-79 30.26704202882312 34.0 29.5 36.0 7.5 38.5 80-81 29.675964349932507 34.0 29.0 35.5 6.5 37.0 82-83 29.112225147930573 34.0 29.0 35.0 2.0 37.0 84-85 28.52195048260849 33.5 27.5 35.0 2.0 36.0 86-87 27.910617720188455 33.0 26.5 35.0 2.0 36.0 88-89 27.352877109578003 33.0 26.0 35.0 2.0 35.5 90-91 26.772721148869643 32.0 25.0 35.0 2.0 35.0 92-93 26.09730670316243 32.0 24.5 34.0 2.0 35.0 94-95 25.282884872931774 31.5 21.0 34.0 2.0 35.0 96-97 24.374719545510327 31.0 12.5 34.0 2.0 35.0 98-99 23.134006890976902 31.0 2.0 34.0 2.0 35.0 100-101 19.92003745618107 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18672566897250675 1101 2 0.23256395987972311 1101 3 0.16376265662232825 1101 4 0.08996368616205075 1101 5 0.09808056208176197 1101 6 0.0643692163490428 1101 7 0.13727897411498446 1101 8 0.03957454177005104 1101 9 0.20168732342635565 1101 10-11 0.289265525168112 1101 12-13 0.22929084244585596 1101 14-15 0.40661226844073184 1101 16-17 0.4290756473395234 1101 18-19 0.41982117427988896 1101 20-21 0.42991554068030524 1101 22-23 0.3604266210065603 1101 24-25 0.424524252411139 1101 26-27 0.24938148070691568 1101 28-29 0.4015920903109418 1101 30-31 0.34252020657061877 1101 32-33 0.39743131895195205 1101 34-35 0.4490537915522097 1101 36-37 0.34416306396816765 1101 38-39 0.3497622671781997 1101 40-41 0.5086125544051754 1101 42-43 0.3515911111650567 1101 44-45 0.5072669234223497 1101 46-47 0.7028735794184442 1101 48-49 0.6250194170286676 1101 50-51 0.3829389421854117 1101 52-53 0.3597236105891355 1101 54-55 0.5295362055621311 1101 56-57 0.6275843856691523 1101 58-59 0.6393239074449539 1101 60-61 0.5667483008553234 1101 62-63 0.5571466222715671 1101 64-65 0.4567440854388778 1101 66-67 0.6165171641777718 1101 68-69 0.6794751344654841 1101 70-71 0.5338237503265617 1101 72-73 0.58561398889481 1101 74-75 0.658771689330429 1101 76-77 0.6273214864691745 1101 78-79 0.8711039930581919 1101 80-81 0.9211475568362069 1101 82-83 0.9023674549700438 1101 84-85 0.9753044502024117 1101 86-87 1.065122435979049 1101 88-89 1.113966280101863 1101 90-91 1.1203454698247164 1101 92-93 1.4166975894857536 1101 94-95 1.4560470901634055 1101 96-97 1.5294633673932658 1101 98-99 1.791172292049339 1101 100-101 2.0725833309387696 1102 1 0.19406408479131443 1102 2 0.20795599268974385 1102 3 0.12131768341905413 1102 4 0.11812605699025625 1102 5 0.06037200098913331 1102 6 0.1097227908604097 1102 7 -0.03998742523359766 1102 8 -0.02453130278632898 1102 9 0.17578118486235894 1102 10-11 0.14311368734873042 1102 12-13 0.22248331146682432 1102 14-15 0.300293663265073 1102 16-17 0.28946040694798825 1102 18-19 0.32164948324693654 1102 20-21 0.227816434025101 1102 22-23 0.30616643150479206 1102 24-25 0.3199347308935998 1102 26-27 0.2961771281984795 1102 28-29 0.42515447816732177 1102 30-31 0.34638958602975123 1102 32-33 0.1959593334584966 1102 34-35 0.2820603365278558 1102 36-37 0.5345108099550018 1102 38-39 0.3844450450883201 1102 40-41 0.5150371780419079 1102 42-43 0.4992132135460068 1102 44-45 0.5352616166118978 1102 46-47 0.5863330917788119 1102 48-49 0.6554252263512268 1102 50-51 0.46581981384646554 1102 52-53 0.4638989762645167 1102 54-55 0.5674833195140891 1102 56-57 0.6211208146268277 1102 58-59 0.7340007481401045 1102 60-61 0.7408816984367803 1102 62-63 0.5730960377434755 1102 64-65 0.6299336877496344 1102 66-67 0.6654045364325398 1102 68-69 0.7680238726148261 1102 70-71 0.828889634844785 1102 72-73 0.8078342989704943 1102 74-75 0.8867388928976503 1102 76-77 1.0058433961963331 1102 78-79 0.9802497949354603 1102 80-81 1.1475684125378791 1102 82-83 1.1931933935798753 1102 84-85 1.2263567094689485 1102 86-87 1.2724258897081704 1102 88-89 1.2328980705140964 1102 90-91 1.3306060958997357 1102 92-93 1.50083972306086 1102 94-95 1.3983642841749209 1102 96-97 1.4059579740833392 1102 98-99 1.648473300243694 1102 100-101 1.496321027408051 1103 1 0.06617807258407993 1103 2 0.056882356912478116 1103 3 0.07473094371567512 1103 4 -0.12033946398004502 1103 5 -0.0961176626707001 1103 6 -0.023918977102582062 1103 7 -0.0062347432973623995 1103 8 -0.022102009248044396 1103 9 -0.017297649229860212 1103 10-11 -0.11506538082077356 1103 12-13 -0.1065917004475736 1103 14-15 -0.002040937109676122 1103 16-17 -0.04207930676189875 1103 18-19 0.001616256903965052 1103 20-21 -0.060783590473583615 1103 22-23 -0.1319444805544947 1103 24-25 -0.1335481496925226 1103 26-27 -0.16909478807725975 1103 28-29 -0.32851817153783713 1103 30-31 -0.29094995236140875 1103 32-33 -0.3082657329080618 1103 34-35 -0.0408330635643992 1103 36-37 -0.12392487238125227 1103 38-39 -0.12773180671568696 1103 40-41 -0.05728873295512926 1103 42-43 -0.1874406965782569 1103 44-45 0.028283558247601093 1103 46-47 -0.009646970501442809 1103 48-49 0.06856326879137242 1103 50-51 0.15497957590297773 1103 52-53 -0.08209611060054556 1103 54-55 0.20844000277478614 1103 56-57 0.15444118088218062 1103 58-59 0.13353460321889088 1103 60-61 -0.04128239682482615 1103 62-63 0.07567509863992683 1103 64-65 -0.012611342940708425 1103 66-67 0.2680444612521953 1103 68-69 0.32211955529560043 1103 70-71 0.5319902019510891 1103 72-73 0.4549436454106264 1103 74-75 0.5937338898066038 1103 76-77 0.36299308891008053 1103 78-79 0.368842020047758 1103 80-81 0.3531317674798231 1103 82-83 0.4992561484954017 1103 84-85 0.5645845166260095 1103 86-87 0.7106418303141702 1103 88-89 0.8274429021526188 1103 90-91 0.5354914305651839 1103 92-93 0.7336688715209831 1103 94-95 0.7130018184587286 1103 96-97 0.7381936514433001 1103 98-99 0.8445422181099325 1103 100-101 0.8934014252054645 1104 1 0.20713409710174702 1104 2 0.2206762878296935 1104 3 0.1364913342431926 1104 4 0.15245387730644921 1104 5 0.1942891825306674 1104 6 0.19826210809220868 1104 7 0.18748637508129207 1104 8 0.16907546960066355 1104 9 0.3296109031581409 1104 10-11 0.29217747847688 1104 12-13 0.23047139004017225 1104 14-15 0.5052506010037803 1104 16-17 0.45794126175181304 1104 18-19 0.5063915801724406 1104 20-21 0.38686268593981765 1104 22-23 0.41024839725481854 1104 24-25 0.26239231131642526 1104 26-27 0.2576195975412361 1104 28-29 0.43033657361827693 1104 30-31 0.4510214711168672 1104 32-33 0.4222353160861374 1104 34-35 0.4908561599056469 1104 36-37 0.49451548578512927 1104 38-39 0.6381916509885883 1104 40-41 0.6886078485578864 1104 42-43 0.635886054830685 1104 44-45 0.5751214920718368 1104 46-47 0.8614124450785141 1104 48-49 0.8487304222461987 1104 50-51 0.6882114379019626 1104 52-53 0.7238022535169932 1104 54-55 0.9340183208188577 1104 56-57 1.0473272176038293 1104 58-59 1.0873720022617732 1104 60-61 1.1877445753528235 1104 62-63 1.0514659579924341 1104 64-65 1.0839509727996912 1104 66-67 1.100325372300233 1104 68-69 1.1221078998044902 1104 70-71 0.9768768694404955 1104 72-73 1.2978996150789293 1104 74-75 1.3133027737590446 1104 76-77 1.310690786607779 1104 78-79 1.2328258669083212 1104 80-81 1.3837137192946471 1104 82-83 1.3655333846356505 1104 84-85 1.2795631939334555 1104 86-87 1.2647815865087217 1104 88-89 1.2655522443715483 1104 90-91 1.3680191637778485 1104 92-93 1.6054756097660778 1104 94-95 1.6114413538845618 1104 96-97 1.7896903536249376 1104 98-99 1.8022225011326825 1104 100-101 1.7882311864735811 1105 1 0.08338683979284411 1105 2 -0.0775637775336584 1105 3 0.07800694699168176 1105 4 0.023827430186855736 1105 5 -0.03042534697838306 1105 6 0.026642948459347338 1105 7 0.0929846059219912 1105 8 0.02645141430537734 1105 9 0.17202641509420147 1105 10-11 0.1003797721243771 1105 12-13 0.09383924998336823 1105 14-15 0.10775229768356098 1105 16-17 0.08286219317960075 1105 18-19 0.21272184300955388 1105 20-21 0.25643866424866957 1105 22-23 0.2788664927564781 1105 24-25 0.12751079886641747 1105 26-27 0.24292437394190358 1105 28-29 0.1710991405794715 1105 30-31 0.3152479663365071 1105 32-33 0.24860526146293438 1105 34-35 0.4366184138870821 1105 36-37 0.42963436867798777 1105 38-39 0.3696080656241847 1105 40-41 0.3685835679171703 1105 42-43 0.3541569075193465 1105 44-45 0.3109542159182652 1105 46-47 0.4720091986547317 1105 48-49 0.5509750637031701 1105 50-51 0.5428764012998002 1105 52-53 0.506635478131038 1105 54-55 0.7274842093189946 1105 56-57 0.7745361134771116 1105 58-59 0.7267390089232961 1105 60-61 0.7587481532057154 1105 62-63 0.6862564592212905 1105 64-65 0.7324112770819156 1105 66-67 0.6157948090025442 1105 68-69 0.5130978712739136 1105 70-71 0.6398293722902579 1105 72-73 0.6682559837229682 1105 74-75 0.6390389351116532 1105 76-77 0.5897913157083075 1105 78-79 0.6874512136569528 1105 80-81 0.8273291791772337 1105 82-83 0.9357112724505257 1105 84-85 0.9029772925187878 1105 86-87 0.7407327979051388 1105 88-89 1.0416489913587057 1105 90-91 1.1589948040685556 1105 92-93 1.1793190671711784 1105 94-95 1.2611749916319006 1105 96-97 1.5000382882879393 1105 98-99 1.8455578441255582 1105 100-101 1.7193076886117282 1106 1 0.023961801554246875 1106 2 -0.5395791632778568 1106 3 -0.059206122424779295 1106 4 0.05425886316065487 1106 5 -0.040614310557181454 1106 6 -0.04962329941707111 1106 7 -0.003132553754490175 1106 8 0.004848017701981178 1106 9 0.06976920902344119 1106 10-11 -0.37859781808202797 1106 12-13 -0.20110309580643104 1106 14-15 -0.11308124730252445 1106 16-17 -0.08580882506769427 1106 18-19 0.019235244861931733 1106 20-21 -0.08185616239361337 1106 22-23 -0.010228240194194882 1106 24-25 -0.04059404826384849 1106 26-27 0.09479002241771894 1106 28-29 0.28189406599304334 1106 30-31 0.19358608302208324 1106 32-33 0.21383459427701723 1106 34-35 0.32573918012649017 1106 36-37 0.2529745172300011 1106 38-39 0.33756595519223964 1106 40-41 0.37295278626943684 1106 42-43 0.19763440459853854 1106 44-45 0.26947944774858 1106 46-47 0.3283742319265386 1106 48-49 0.5728292688200867 1106 50-51 0.26695987432395185 1106 52-53 0.2962300643357949 1106 54-55 0.46473671669862426 1106 56-57 0.23111237619743719 1106 58-59 0.19347883689194134 1106 60-61 0.32756069659453146 1106 62-63 0.39595071976301455 1106 64-65 0.5267673447430639 1106 66-67 0.7940600465898129 1106 68-69 0.8763723087008941 1106 70-71 0.7219905021208817 1106 72-73 0.7272485448341719 1106 74-75 0.4569794691538789 1106 76-77 0.6122504949471477 1106 78-79 0.8144633258385596 1106 80-81 0.6073821064759244 1106 82-83 0.576279390772882 1106 84-85 0.47519003740102406 1106 86-87 0.2666486193718036 1106 88-89 0.6757641699654116 1106 90-91 0.6705834978606369 1106 92-93 0.9236495954000112 1106 94-95 0.841846438828771 1106 96-97 0.7167657698459315 1106 98-99 0.8111692101606565 1106 100-101 0.4269542087582501 1107 1 0.1090075344366781 1107 2 0.16290740509690593 1107 3 0.0554159989508598 1107 4 0.025872300483648303 1107 5 -0.06576404427080718 1107 6 0.09086161325641484 1107 7 0.05268535932454199 1107 8 -0.10687830225056416 1107 9 0.09227786458729526 1107 10-11 0.022672614243695932 1107 12-13 0.14290361749247182 1107 14-15 0.1988839595093097 1107 16-17 0.23723181670089133 1107 18-19 0.09681942922795628 1107 20-21 0.17331329561558562 1107 22-23 0.2912602151437724 1107 24-25 0.2591301970346578 1107 26-27 0.30977772196491316 1107 28-29 0.20881865114603215 1107 30-31 0.24754258902316195 1107 32-33 0.17508104574720562 1107 34-35 0.31866139233108726 1107 36-37 0.4353940866806383 1107 38-39 0.32477532766804984 1107 40-41 0.40483341489966307 1107 42-43 0.4857109254808165 1107 44-45 0.4044228723689187 1107 46-47 0.6861133785365396 1107 48-49 0.5639474704737708 1107 50-51 0.5811078249746728 1107 52-53 0.5275070658406733 1107 54-55 0.797290099658305 1107 56-57 0.9535519883477406 1107 58-59 0.8216230772970832 1107 60-61 0.9000361711004246 1107 62-63 1.1274087088568336 1107 64-65 0.8479130632135181 1107 66-67 1.0939786859653466 1107 68-69 1.1061561625969887 1107 70-71 1.120506739053745 1107 72-73 1.142308388045297 1107 74-75 1.1686519955730397 1107 76-77 1.146606715390032 1107 78-79 1.040916086402035 1107 80-81 1.2782947196350634 1107 82-83 1.3196440170837853 1107 84-85 1.29994712859531 1107 86-87 1.1361293358145268 1107 88-89 1.2023827520281998 1107 90-91 0.9947096462101328 1107 92-93 1.0581556333470736 1107 94-95 1.0764063540997668 1107 96-97 0.8389386766741893 1107 98-99 1.1893561837465185 1107 100-101 1.1001300046846865 1108 1 -2.6168466789044942E-5 1108 2 -0.08335680863343597 1108 3 0.03773459153527625 1108 4 -0.08980893344951113 1108 5 -0.09057991633540752 1108 6 -0.06059445626272009 1108 7 0.012200870230280714 1108 8 -0.21076076002336208 1108 9 -0.05137543867574834 1108 10-11 -0.016169706004859563 1108 12-13 -0.08729671527836302 1108 14-15 -0.1315596429320678 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72-73 0.21105221299460553 2316 74-75 0.4018780995901601 2316 76-77 0.3354327416776002 2316 78-79 0.240644150046613 2316 80-81 0.30021819817280715 2316 82-83 0.42768457692383066 2316 84-85 0.33835418793666605 2316 86-87 0.5869156451125725 2316 88-89 0.7615204222957104 2316 90-91 0.8755692132659156 2316 92-93 1.065517113205292 2316 94-95 1.3178689807029365 2316 96-97 1.3354917692332222 2316 98-99 1.862084350406164 2316 100-101 2.339347371151412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5526.0 3 878.0 4 851.0 5 956.0 6 1113.0 7 1358.0 8 1737.0 9 2163.0 10 2768.0 11 3402.0 12 4262.0 13 5395.0 14 6298.0 15 7400.0 16 9171.0 17 11319.0 18 10298.0 19 8761.0 20 9257.0 21 10188.0 22 11372.0 23 13064.0 24 15057.0 25 17456.0 26 20375.0 27 24349.0 28 28902.0 29 35192.0 30 43224.0 31 53004.0 32 67908.0 33 87904.0 34 117709.0 35 159620.0 36 221557.0 37 277898.0 38 185571.0 39 16088.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73749985786362 19.28780643767437 14.635632553712503 23.33906115074951 2 14.919698853986976 20.24284243323839 40.691619529024756 24.145839183749885 3 18.709017859471082 24.26209581170631 29.981058035424752 27.047828293397856 4 12.565191227600964 16.67604974592137 37.83250869216368 32.92625033431399 5 13.737764108050282 37.32883123829901 34.7689890345012 14.164415619149507 6 32.04063189725587 36.60694113762361 17.704165457941027 13.648261507179496 7 28.527101314424446 32.411618592314525 21.09268884824761 17.96859124501342 8 25.914807573402847 35.61952507478151 20.147524272655872 18.318143079159775 9 25.867443565946303 17.1156277220945 20.442741671853202 36.574187040106 10-11 24.563366364434984 26.984306326808678 28.02681843769263 20.425508871063712 12-13 25.33590265115494 23.93667177521923 28.437668695046153 22.289756878579677 14-15 22.17982376248544 25.818010451255603 26.128606928176445 25.87355885808251 16-17 22.200710262307773 29.210222536824887 26.6767464291372 21.912320771730144 18-19 22.081627510863182 27.94365280320754 28.680641628740627 21.29407805718865 20-21 22.62917962080509 27.194650780489972 28.70256095087136 21.47360864783358 22-23 22.350467038301115 26.99170923887344 28.55264745266874 22.1051762701567 24-25 22.22677464599985 27.194055678222213 28.625963765008933 21.953205910769004 26-27 22.10031562713558 27.557941556347547 28.375965553366715 21.965777263150155 28-29 22.034938905376862 27.62462902061442 28.556288601909042 21.784143472099675 30-31 22.199441063109866 27.328912065208634 28.47880722693834 21.99283964474316 32-33 21.992195920822855 27.463540578705057 28.369248251602176 22.17501524886991 34-35 22.51608196076426 27.39473071175732 28.183629972690667 21.905557354787756 36-37 22.353093333894783 27.27676800488728 28.246303709875185 22.12383495134275 38-39 22.187487468755428 27.462640182855917 28.403385775199496 21.94648657318915 40-41 22.422342378226762 27.317899438981136 28.311185858675643 21.948572324116462 42-43 22.291507855373304 27.338958246930524 28.122607387068673 22.246926510627503 44-45 22.329463448182292 27.55576333960807 27.938596916278215 22.176176295931423 46-47 22.217068010089037 27.664672900069814 27.742843879620033 22.375415210221114 48-49 22.46934863083198 27.6918125316674 27.626205032292393 22.212633805208227 50-51 22.42198347582576 27.704197359528145 27.610572850964893 22.26324631368121 52-53 22.608951451961744 27.71861325702748 27.472680655329988 22.199754635680787 54-55 22.395030501214567 27.55667423793755 27.606373236470976 22.441922024376908 56-57 22.295704163508848 27.974999431926783 27.52688991275732 22.202406491807047 58-59 22.503787380121985 27.533100733284904 27.65589404557687 22.307217841016243 60-61 22.45130700063153 27.484637772201303 27.97609540453031 22.087959822636858 62-63 22.304103146573315 27.63235587492569 27.892434463236626 22.17110651526437 64-65 22.284843049567094 27.469299570434565 27.804672025319498 22.44118535467884 66-67 22.101543586821958 27.74570163113304 27.656985904056697 22.495768877988308 68-69 22.14882434375469 27.538762221949703 27.818211770162353 22.494201664133254 70-71 22.18011508936451 27.495638453254013 27.784681773329222 22.53956468405226 72-73 22.196473110575855 27.431536695101705 27.803493473129286 22.56849672119315 74-75 22.04071539325242 27.634336737421805 27.635573170079663 22.68937469924611 76-77 22.211520562837475 27.52658770089701 27.626177744165208 22.635713992100303 78-79 22.14910353203266 27.514543437815714 27.622021339844395 22.714331690307237 80-81 22.070872641115354 27.711409866963415 27.55040251837148 22.66731497354975 82-83 22.184733152975898 27.54175666716568 27.6397658739494 22.633744305909023 84-85 22.232316482341542 27.295399111229912 27.48835577533496 22.98392863109359 86-87 22.32703540920023 27.213033248040823 27.711790614794385 22.74814072796456 88-89 22.262948729044496 26.983774261166992 27.717419453577186 23.035857556211322 90-91 22.069710565719596 27.087803340939796 27.966829188095694 22.875656905244917 92-93 22.094629208143957 26.916381982829744 27.985153555504244 23.003835253522055 94-95 21.87429200786736 27.042509267804977 27.90529897233793 23.17789975198973 96-97 21.970600265739165 27.160534258697343 27.833804748550335 23.035060727013164 98-99 21.889738570981244 27.16650831901632 27.60669584986571 23.337057260136728 100-101 22.258075726075223 26.545593427280874 28.074128873464865 23.122201973179042 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2060.0 1 1139.0 2 205.5 3 163.5 4 144.5 5 146.0 6 172.5 7 308.5 8 478.5 9 594.5 10 624.5 11 643.5 12 704.0 13 785.0 14 880.0 15 944.5 16 1046.5 17 1165.5 18 1268.5 19 1394.5 20 1523.0 21 1784.0 22 2184.0 23 2651.0 24 3358.0 25 4374.0 26 5823.0 27 7900.0 28 10219.5 29 12935.0 30 16296.5 31 19844.5 32 23497.0 33 27303.0 34 31480.0 35 34898.0 36 38521.0 37 42217.0 38 45482.0 39 47621.0 40 49321.5 41 53046.0 42 56123.5 43 58527.5 44 60528.5 45 61519.0 46 60845.0 47 60317.0 48 62480.5 49 62441.5 50 60138.0 51 58104.0 52 54348.5 53 50526.0 54 47365.5 55 43040.0 56 37771.5 57 32537.0 58 28227.5 59 24361.0 60 20124.0 61 16047.5 62 12416.0 63 9173.0 64 6771.0 65 5101.0 66 3765.0 67 2948.0 68 2170.0 69 1433.5 70 1043.0 71 785.5 72 616.0 73 469.0 74 337.5 75 250.0 76 192.0 77 123.0 78 67.5 79 53.0 80 37.5 81 25.0 82 18.5 83 16.5 84 10.5 85 5.5 86 7.5 87 7.0 88 4.5 89 1.0 90 2.5 91 4.0 92 2.0 93 1.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28739081950069095 2 0.24890752805211852 3 0.24884083257300485 4 0.2502414376343914 5 0.2502414376343914 6 0.2191613443674334 7 0.2224961183231156 8 0.2218958590110928 9 0.23283391758573035 10-11 0.23943677001798108 12-13 0.23306735176262813 14-15 0.22406346208228622 16-17 0.21909464888831975 18-19 0.2086568064070345 20-21 0.213992444736126 22-23 0.21132462557158027 24-25 0.2135255763823305 26-27 0.20839002449057992 28-29 0.22089542682438815 30-31 0.23186683313858253 32-33 0.22272955250001333 34-35 0.2229963344164679 36-37 0.21499287692283067 38-39 0.2062224214193865 40-41 0.20385473191085215 42-43 0.21412583569435328 44-45 0.20945715215639824 46-47 0.21826095539939921 48-49 0.22236272736488827 50-51 0.2323003537528212 52-53 0.2412375479540495 54-55 0.22316307311425204 56-57 0.20448833896243176 58-59 0.19575123119854443 60-61 0.1992193961124539 62-63 0.20498855505578412 64-65 0.19715183625993096 66-67 0.20005308960137444 68-69 0.20125360822542004 70-71 0.20252082232857926 72-73 0.20515529375356822 74-75 0.20755633100165938 76-77 0.21465939952726243 78-79 0.21545974527662617 80-81 0.20989067277063694 82-83 0.20435494800420448 84-85 0.19355028038779418 86-87 0.19561784024031714 88-89 0.20385473191085215 90-91 0.20252082232857926 92-93 0.20685602847096612 94-95 0.20295434294281797 96-97 0.20995736824975056 98-99 0.21152471200892117 100-101 0.19908600515422661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1499352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.33054659891415 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62413769508031 46.83106656413571 2 12.929578295055439 15.600970516683022 3 4.1948909329219815 7.592401887180363 4 1.8854468260409651 4.550001503929569 5 0.959304394912862 2.893767924991679 6 0.5537681779467336 2.004548211876672 7 0.3834044768732861 1.6191701160765262 8 0.23984105719852064 1.1575793662118548 9 0.18542900717583702 1.0068330022390999 >10 0.9483516994758141 10.281524361823957 >50 0.06550506012757969 2.7335451754982696 >100 0.029456518269293408 2.998140675666748 >500 4.4292946064667857E-4 0.16073389144044176 >1k 4.4292946064667857E-4 0.5697168022460878 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2878 0.19194958888906674 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2335 0.15573394373035818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.1331908717899466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.669547911364376E-5 0.0 8 0.0 0.0 0.0 6.669547911364376E-5 0.0 9 0.0 0.0 0.0 6.669547911364376E-5 0.0 10-11 0.0 0.0 0.0 6.669547911364376E-5 0.0 12-13 0.0 0.0 0.0 6.669547911364376E-5 0.0 14-15 0.0 0.0 0.0 6.669547911364376E-5 0.0 16-17 0.0 0.0 0.0 6.669547911364376E-5 0.0 18-19 0.0 0.0 0.0 6.669547911364376E-5 0.0 20-21 0.0 0.0 0.0 6.669547911364376E-5 0.0 22-23 0.0 0.0 0.0 1.667386977841094E-4 0.0 24-25 0.0 0.0 0.0 5.002160933523282E-4 0.0 26-27 0.0 0.0 0.0 6.669547911364376E-4 0.0 28-29 0.0 0.0 0.0 0.0013005618427160534 0.0 30-31 0.0 0.0 0.0 0.0028679056018866816 0.0 32-33 0.0 0.0 0.0 0.005602420245546076 0.0 34-35 0.0 0.0 0.0 0.007770023316739498 0.0 36-37 0.0 0.0 0.0 0.012138577198683165 0.0 38-39 0.0 0.0 0.0 0.018174518058467926 0.0 40-41 0.0 0.0 0.0 0.026511452947673396 0.0 42-43 0.0 0.0 0.0 0.03881676884414067 0.0 44-45 0.0 0.0 0.0 0.05038843447035786 0.0 46-47 0.0 0.0 0.0 0.0717976832658375 0.0 48-49 0.0 0.0 0.0 0.09967639353534061 0.0 50-51 0.0 0.0 0.0 0.11514974468970596 0.0 52-53 0.0 0.0 0.0 0.1423281524285158 0.0 54-55 0.0 0.0 0.0 0.18341256756252033 0.0 56-57 0.0 0.0 0.0 0.25644411719196025 0.0 58-59 0.0 0.0 0.0 0.35305251868807325 0.0 60-61 0.0 0.0 0.0 0.4620329315597671 0.0 62-63 0.0 0.0 0.0 0.5950904123914864 0.0 64-65 0.0 0.0 0.0 0.7507576606427311 0.0 66-67 0.0 0.0 0.0 0.9508107502441054 0.0 68-69 0.0 0.0 0.0 1.2036866593034858 0.0 70-71 0.0 0.0 0.0 1.3578199115351164 0.0 72-73 0.0 0.0 0.0 1.54266643189858 0.0 74-75 0.0 0.0 0.0 1.7719988368308441 0.0 76-77 0.0 0.0 0.0 2.038280537192067 0.0 78-79 0.0 0.0 0.0 2.312332260870029 0.0 80-81 0.0 0.0 0.0 2.6962647863877196 0.0 82-83 0.0 6.669547911364376E-5 0.0 3.106241896499288 0.0 84-85 0.0 6.669547911364376E-5 0.0 3.6423401576147563 0.0 86-87 0.0 6.669547911364376E-5 0.0 4.361684247594961 0.0 88-89 0.0 6.669547911364376E-5 0.0 5.142254787401491 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2545 0.0 40.52902 1 GTATCAA 4035 0.0 34.63362 1 ATCAACG 4730 0.0 29.0316 3 TAGGCCG 115 3.8558555E-6 28.922411 5 TCAACGC 4820 0.0 28.390938 4 CAACGCA 4935 0.0 27.729343 5 TATCAAC 5055 0.0 27.542908 2 AACGCAG 5065 0.0 27.106005 6 ACGCAGA 5775 0.0 23.362967 7 CGCAGAG 5845 0.0 22.920614 8 GTACATG 4465 0.0 22.142977 1 TATACCG 110 0.0028723627 21.597904 5 ACATGGG 4505 0.0 21.199984 3 GTATAGG 405 0.0 21.125723 1 TACATGG 4615 0.0 21.107922 2 GCAGAGT 6515 0.0 20.198866 9 TCGCGCT 165 2.7284841E-11 20.145576 60-61 GAGTACT 3045 0.0 19.88862 12-13 GTGTAGC 680 0.0 19.572361 1 AGAGTAC 5475 0.0 19.522953 10-11 >>END_MODULE