FastQCFastQC Report
Thu 26 May 2016
SRR937243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499352
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37750.2517754336540052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.19048228834856656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.13219043960324195No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.044.2453231
GTATCAA42300.039.7677461
ATCAACG52150.031.5128043
TCAACGC52700.031.1839244
TATCAAC53500.030.717622
CAACGCA53650.030.6317395
AACGCAG55250.029.7446636
ACGCAGA62900.026.1288137
CGCAGAG63150.025.9501558
GTACATG43300.023.2656961
GCAGAGT70650.022.5902659
GAGTACT38850.021.64095712-13
TACATGG45250.021.6228962
ACATGGG43600.021.2428783
AGTACTT42450.020.42111212-13
AGAGTAC62200.020.08443310-11
TAGACTG6750.019.7024175
GTACTTT43150.019.48308614-15
AGGATCG2201.3461486E-619.430555
CAGAGTA67000.018.85823410-11
Produced by FastQC (version 0.11.5)