##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937243_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1499352 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27152996761267 33.0 31.0 34.0 31.0 34.0 2 32.48521694705446 34.0 31.0 34.0 31.0 34.0 3 32.56446785011125 34.0 31.0 34.0 31.0 34.0 4 36.17274729349746 37.0 35.0 37.0 35.0 37.0 5 36.09482096265587 37.0 35.0 37.0 35.0 37.0 6 36.12294844706246 37.0 35.0 37.0 35.0 37.0 7 36.06320196991767 37.0 35.0 37.0 35.0 37.0 8 36.037459515844176 37.0 35.0 37.0 35.0 37.0 9 37.7618291101756 39.0 37.0 39.0 35.0 39.0 10-11 37.67846743126364 39.0 37.0 39.0 35.0 39.0 12-13 37.574343449703605 39.0 37.0 39.0 35.0 39.0 14-15 38.85539286305017 40.0 38.0 41.0 35.0 41.0 16-17 38.80610723832696 40.0 38.0 41.0 35.0 41.0 18-19 38.76304563571463 40.0 38.0 41.0 35.0 41.0 20-21 38.64705686189767 40.0 38.0 41.0 34.0 41.0 22-23 38.59798299532065 40.0 38.0 41.0 34.0 41.0 24-25 38.6336684114204 40.0 38.0 41.0 34.0 41.0 26-27 38.49795244879121 40.0 38.0 41.0 34.0 41.0 28-29 38.35799965585133 40.0 38.0 41.0 34.0 41.0 30-31 38.4509161291011 40.0 38.0 41.0 34.0 41.0 32-33 38.403816115228445 40.0 38.0 41.0 34.0 41.0 34-35 38.29358582907817 40.0 38.0 41.0 34.0 41.0 36-37 38.161848251778096 40.0 38.0 41.0 34.0 41.0 38-39 38.07166696012678 40.0 38.0 41.0 33.5 41.0 40-41 37.900375962415765 40.0 37.5 41.0 33.0 41.0 42-43 37.7382916086416 40.0 37.0 41.0 33.0 41.0 44-45 37.50255943901099 40.0 37.0 41.0 33.0 41.0 46-47 37.242264324855 40.0 37.0 41.0 32.0 41.0 48-49 36.99903058121109 40.0 36.5 41.0 31.0 41.0 50-51 36.7946742992973 39.0 36.0 41.0 31.0 41.0 52-53 36.574093341656926 39.0 36.0 40.5 31.0 41.0 54-55 36.36832878470166 39.0 35.5 40.0 30.0 41.0 56-57 36.09517478217256 39.0 35.0 40.0 30.0 41.0 58-59 35.84354307727605 38.0 35.0 40.0 29.0 41.0 60-61 35.506312727098106 38.0 34.5 40.0 28.5 41.0 62-63 35.150671089910844 38.0 34.0 40.0 28.0 41.0 64-65 34.74720145769639 37.0 34.0 40.0 28.0 41.0 66-67 34.32741444303939 36.5 34.0 39.0 26.5 40.5 68-69 33.88514404889579 36.0 33.0 39.0 26.0 40.0 70-71 33.44856477998495 35.5 33.0 38.5 26.0 40.0 72-73 32.96546374700537 35.0 32.0 38.0 25.5 40.0 74-75 32.48783941329321 35.0 32.0 37.0 24.5 39.0 76-77 31.13857686520577 34.0 30.5 36.0 23.0 38.5 78-79 31.809478027841358 34.0 31.0 36.0 24.0 38.5 80-81 31.630322632710666 34.0 31.0 36.0 24.0 37.5 82-83 31.23375498215229 34.0 31.0 35.5 22.5 37.0 84-85 30.809285277906724 34.0 31.0 35.0 20.5 36.5 86-87 30.436979441785518 34.0 30.5 35.0 19.5 36.0 88-89 30.00702670220202 34.0 30.0 35.0 18.0 36.0 90-91 29.475707172165045 34.0 29.0 35.0 11.0 35.0 92-93 28.993398814954727 34.0 29.0 35.0 4.5 35.0 94-95 28.52566141906637 33.5 29.0 35.0 2.0 35.0 96-97 27.880195244345558 33.0 28.0 35.0 2.0 35.0 98-99 26.91208535420635 33.0 26.0 34.5 2.0 35.0 100-101 23.88572129826752 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25762315789570067 1101 2 0.26487957533388595 1101 3 0.24210291306328457 1101 4 0.10279221486668177 1101 5 0.15270361539296573 1101 6 0.167988997638723 1101 7 0.19035285164757454 1101 8 0.20063207410119333 1101 9 0.2736410844381538 1101 10-11 0.19726273903216907 1101 12-13 0.24545824233329938 1101 14-15 0.3690060119053058 1101 16-17 0.3117209568384993 1101 18-19 0.321965029774951 1101 20-21 0.46618582764959626 1101 22-23 0.4245788455824382 1101 24-25 0.32058372474285335 1101 26-27 0.30465834243377543 1101 28-29 0.22167988171956665 1101 30-31 0.34463523986375577 1101 32-33 0.34166979082183246 1101 34-35 0.3703409252759968 1101 36-37 0.4360030154631147 1101 38-39 0.40894885720416596 1101 40-41 0.36880029480107623 1101 42-43 0.3846493829457316 1101 44-45 0.43636958574531803 1101 46-47 0.5229273330016966 1101 48-49 0.5432753224484372 1101 50-51 0.5809870759700075 1101 52-53 0.4861267195214083 1101 54-55 0.3749175996355163 1101 56-57 0.43742330212520386 1101 58-59 0.38173023256793925 1101 60-61 0.4992487041419622 1101 62-63 0.5817552510431199 1101 64-65 0.5604416406086656 1101 66-67 0.449879266123709 1101 68-69 0.5514369350322994 1101 70-71 0.45161898676536794 1101 72-73 0.5513697661503016 1101 74-75 0.6030135270521981 1101 76-77 0.674389821951916 1101 78-79 0.7161721250364188 1101 80-81 0.614195224532061 1101 82-83 0.6709012483929548 1101 84-85 0.7290251804167198 1101 86-87 0.8485268910500778 1101 88-89 1.0492917872151502 1101 90-91 1.1927005692576849 1101 92-93 1.266216384664066 1101 94-95 1.1816423709073298 1101 96-97 1.566853870892615 1101 98-99 2.0328611833235932 1101 100-101 2.591704085254822 1102 1 0.21375388712699106 1102 2 0.24531393853927597 1102 3 0.21073281579440817 1102 4 0.14090179837399575 1102 5 0.13458469356584146 1102 6 0.1827534609389474 1102 7 0.13489310469702076 1102 8 0.13612022548523584 1102 9 0.31039633984708814 1102 10-11 0.14730544531528267 1102 12-13 0.1787129185127938 1102 14-15 0.26470327462729415 1102 16-17 0.22171644618548214 1102 18-19 0.2275639910567051 1102 20-21 0.2129984806653482 1102 22-23 0.23417969807774597 1102 24-25 0.09456672193592652 1102 26-27 0.19975192036888956 1102 28-29 0.02150686013455072 1102 30-31 0.1522378866569838 1102 32-33 0.176835992082097 1102 34-35 0.16083524484217548 1102 36-37 0.16125375070861736 1102 38-39 0.29051455196700715 1102 40-41 0.34415507849352167 1102 42-43 0.4396933889852974 1102 44-45 0.26751627888414475 1102 46-47 0.23499052199714754 1102 48-49 0.4992363360954286 1102 50-51 0.4493636394139813 1102 52-53 0.420785879905921 1102 54-55 0.5098512260103405 1102 56-57 0.5093494234255331 1102 58-59 0.3734693066884702 1102 60-61 0.4912501071543147 1102 62-63 0.37528950619209667 1102 64-65 0.413306720455374 1102 66-67 0.4536649765923215 1102 68-69 0.4909351973439726 1102 70-71 0.4946844011974676 1102 72-73 0.5634345175707551 1102 74-75 0.5482054698303003 1102 76-77 0.5716172421935148 1102 78-79 0.5921649442217181 1102 80-81 0.6444976647228842 1102 82-83 0.641207361414704 1102 84-85 0.687776141647749 1102 86-87 0.7615071782283209 1102 88-89 0.9190958183622051 1102 90-91 0.9667333828997791 1102 92-93 0.9790090449801916 1102 94-95 1.0301647018099231 1102 96-97 1.1916755506489274 1102 98-99 1.4463480132131004 1102 100-101 2.007061636024577 1103 1 0.15245166712496427 1103 2 0.17214899539345652 1103 3 0.13674056350387787 1103 4 0.15439521530909417 1103 5 0.1764051947553682 1103 6 0.09595011839946466 1103 7 0.10810053775148987 1103 8 0.07802675916997259 1103 9 0.11265892748252782 1103 10-11 0.19756028906917322 1103 12-13 0.14657442246416252 1103 14-15 0.16836458412034006 1103 16-17 0.2480070771606222 1103 18-19 0.24941806310197734 1103 20-21 0.23967046814039605 1103 22-23 0.16713464154893387 1103 24-25 0.1352005680191013 1103 26-27 0.06789635543493233 1103 28-29 0.021424320616070247 1103 30-31 0.07050677696219765 1103 32-33 0.03902370560825119 1103 34-35 0.12217684456809508 1103 36-37 0.13485641647189794 1103 38-39 0.22925874549132175 1103 40-41 0.2008552298877504 1103 42-43 0.2639545526346012 1103 44-45 0.19921805714424323 1103 46-47 0.2229402307881614 1103 48-49 0.2276006947179141 1103 50-51 0.23568772640728497 1103 52-53 0.21429401519225166 1103 54-55 0.1744874205222544 1103 56-57 0.17109242838078131 1103 58-59 0.17185325469000645 1103 60-61 0.11044315297259999 1103 62-63 0.18449809336436118 1103 64-65 0.335924845272352 1103 66-67 0.2758612168095951 1103 68-69 0.2800242385840761 1103 70-71 0.16969210488586128 1103 72-73 0.07638317368384406 1103 74-75 -0.03937637587773679 1103 76-77 0.030113262114010553 1103 78-79 0.17587389217541372 1103 80-81 0.42658328763068454 1103 82-83 0.2621894812017942 1103 84-85 0.23339513540249612 1103 86-87 0.44560786591409496 1103 88-89 0.5606766917786423 1103 90-91 0.7239887261971916 1103 92-93 0.7675817955510276 1103 94-95 0.5237024556249246 1103 96-97 0.6933842383040805 1103 98-99 0.9871347368392129 1103 100-101 1.5329311958329548 1104 1 0.1957141076476816 1104 2 0.20465410324102606 1104 3 0.23241843315435062 1104 4 0.16155305808337772 1104 5 0.15177782766225079 1104 6 0.2085832995257988 1104 7 0.2067471295487664 1104 8 0.18174691768464868 1104 9 0.20474416340337598 1104 10-11 0.22177628588791265 1104 12-13 0.20210940950600076 1104 14-15 0.25297659338988865 1104 16-17 0.21553625016925082 1104 18-19 0.27796716014423595 1104 20-21 0.2877562251302592 1104 22-23 0.1750500912452111 1104 24-25 0.0013132560495989765 1104 26-27 0.19816703707547134 1104 28-29 0.18486515871443743 1104 30-31 0.3145453360692514 1104 32-33 0.36197679568462604 1104 34-35 0.29338229659546755 1104 36-37 0.3922885060957597 1104 38-39 0.48413005515715213 1104 40-41 0.4522847415912352 1104 42-43 0.5376117714973034 1104 44-45 0.6174890678810101 1104 46-47 0.526120123009143 1104 48-49 0.6093214565756639 1104 50-51 0.5855032954009332 1104 52-53 0.5822167324300409 1104 54-55 0.6562431488533207 1104 56-57 0.5845092698798098 1104 58-59 0.5859557133130124 1104 60-61 0.7947404639822366 1104 62-63 0.8864980385149934 1104 64-65 0.8235494561846295 1104 66-67 0.8914734043395924 1104 68-69 0.9862750099033377 1104 70-71 1.0759613878674728 1104 72-73 1.103574748820627 1104 74-75 1.0229530768626702 1104 76-77 0.9421931502212999 1104 78-79 0.8426982507805967 1104 80-81 0.9745113526820433 1104 82-83 0.8841847641559788 1104 84-85 0.8571455981940552 1104 86-87 0.9299620831450248 1104 88-89 1.0453051558591646 1104 90-91 1.143720101408018 1104 92-93 1.3534073471300054 1104 94-95 1.5313505146428454 1104 96-97 1.6506017297681481 1104 98-99 1.9967059518898829 1104 100-101 2.623398375341228 1105 1 0.07888946856276391 1105 2 0.06352576177022229 1105 3 0.05268897945229156 1105 4 0.008856819770699076 1105 5 0.018334786684960136 1105 6 -0.006983984534635113 1105 7 -0.002251697600740954 1105 8 0.01607519721840589 1105 9 0.18192299674659296 1105 10-11 0.013727105235986414 1105 12-13 -0.02466337377363459 1105 14-15 0.020878624134383017 1105 16-17 0.10731443646798056 1105 18-19 0.17808049176441187 1105 20-21 0.11766778363770669 1105 22-23 0.10511970453400465 1105 24-25 0.04386491418345173 1105 26-27 -0.05291439037581114 1105 28-29 0.0727166844084266 1105 30-31 0.13378840274382497 1105 32-33 0.08802725461179506 1105 34-35 0.13070241559366735 1105 36-37 0.12270709182256923 1105 38-39 0.04476906100163802 1105 40-41 0.10666201069452796 1105 42-43 -0.1216022704222226 1105 44-45 -0.0271162316900444 1105 46-47 0.07397308182100915 1105 48-49 0.15873231334953175 1105 50-51 0.3026865434060966 1105 52-53 0.26773219363043665 1105 54-55 0.3459657170005457 1105 56-57 0.2865764188647688 1105 58-59 0.23488219271894906 1105 60-61 0.2348719649014086 1105 62-63 0.2865961329623943 1105 64-65 0.3512811106286122 1105 66-67 0.54498586093424 1105 68-69 0.46454886060869427 1105 70-71 0.33298201817576256 1105 72-73 0.09816225796292599 1105 74-75 0.28410051009915094 1105 76-77 0.3768829213836682 1105 78-79 0.39644511274662975 1105 80-81 0.4163506523980516 1105 82-83 0.3863558553681692 1105 84-85 0.5487575757649381 1105 86-87 0.623464293770521 1105 88-89 0.6405910216929733 1105 90-91 0.8474270996355635 1105 92-93 0.827112105081337 1105 94-95 0.8179374373599053 1105 96-97 1.178550410970253 1105 98-99 1.4380873127917901 1105 100-101 1.9242275246292841 1106 1 -0.02779833515893415 1106 2 0.08357280512235121 1106 3 0.03382930805024387 1106 4 0.07704145998923906 1106 5 0.03625330884077016 1106 6 -0.03317248529940997 1106 7 0.011612263297116954 1106 8 0.01205661692863913 1106 9 -0.08899252840621585 1106 10-11 -0.16188259579743658 1106 12-13 -0.31579244244507265 1106 14-15 -0.058845676988219964 1106 16-17 -0.1845222579110839 1106 18-19 -0.0779177472846726 1106 20-21 0.08642889540730181 1106 22-23 0.1018496694843023 1106 24-25 -0.07140858350530266 1106 26-27 0.05558939397543128 1106 28-29 0.031923085352119074 1106 30-31 0.09580978531605666 1106 32-33 0.03585626176283796 1106 34-35 -0.4208981730831951 1106 36-37 -0.06334308681235967 1106 38-39 0.018529763575898528 1106 40-41 -0.16951872946926017 1106 42-43 -0.051148359925939246 1106 44-45 0.08365823395730132 1106 46-47 0.0844823465675617 1106 48-49 0.17638944435412185 1106 50-51 0.21280467386321078 1106 52-53 0.19665708230108692 1106 54-55 0.2572246558018634 1106 56-57 0.31663040442722945 1106 58-59 0.218461466552462 1106 60-61 0.1762720196489198 1106 62-63 0.05329518250042753 1106 64-65 0.005710015057523776 1106 66-67 0.04475233739562867 1106 68-69 0.22253487177266607 1106 70-71 0.3151876656356549 1106 72-73 0.04511396444853233 1106 74-75 0.13760047059063396 1106 76-77 -0.05127190401691806 1106 78-79 0.06731520310824735 1106 80-81 0.09477886260592072 1106 82-83 0.21857448419696723 1106 84-85 0.2484427775687834 1106 86-87 0.4000055491253356 1106 88-89 0.4978792137269288 1106 90-91 0.5977347016380818 1106 92-93 0.6854373956100197 1106 94-95 0.26591565970253583 1106 96-97 0.49220391847969225 1106 98-99 0.812894003276444 1106 100-101 1.2959500817331957 1107 1 0.04263683944381569 1107 2 0.008930506982167685 1107 3 -0.015695934690981517 1107 4 0.03532884817074944 1107 5 0.0059320223541732275 1107 6 0.0348009229315025 1107 7 0.019533821935418416 1107 8 0.06184412409272966 1107 9 0.07367006715690394 1107 10-11 0.0112454395152497 1107 12-13 -0.033574556849337966 1107 14-15 -0.02247471276008639 1107 16-17 0.07223586893439915 1107 18-19 0.11565769605112308 1107 20-21 0.09594833984242968 1107 22-23 0.07772678896628094 1107 24-25 -0.003181708859322896 1107 26-27 0.02580059643685928 1107 28-29 0.20725732585781032 1107 30-31 0.1971967702974382 1107 32-33 0.2154713614903514 1107 34-35 0.08992672812775027 1107 36-37 0.18000310557744825 1107 38-39 0.12878262669900664 1107 40-41 0.15713831245617627 1107 42-43 0.242073738658668 1107 44-45 0.3971894861568046 1107 46-47 0.43405526613198475 1107 48-49 0.4899366167582073 1107 50-51 0.4751080593366126 1107 52-53 0.49882340319207685 1107 54-55 0.4859322200775864 1107 56-57 0.3971899912392658 1107 58-59 0.4170119738204505 1107 60-61 0.5091982705580449 1107 62-63 0.44665453000666133 1107 64-65 0.6122455104104958 1107 66-67 0.8110596543216602 1107 68-69 0.7705497898422706 1107 70-71 0.9192157287668081 1107 72-73 0.7864496025806105 1107 74-75 0.6233104111663792 1107 76-77 0.49189992023742946 1107 78-79 0.6256472516531453 1107 80-81 0.7402928841166272 1107 82-83 0.6172926563563834 1107 84-85 0.5889524922452019 1107 86-87 0.8229685170536669 1107 88-89 1.099679705074717 1107 90-91 1.0349931753764459 1107 92-93 1.1982129408351305 1107 94-95 0.950933993074969 1107 96-97 1.271222680859747 1107 98-99 1.497007398704163 1107 100-101 2.1028845038967994 1108 1 -0.039278406506952024 1108 2 -0.04411174387342243 1108 3 0.06150965775149331 1108 4 -0.033175023641511814 1108 5 -0.02602422853295394 1108 6 -0.029419115865472634 1108 7 -0.022860945203852623 1108 8 0.01247654638049056 1108 9 0.0484973732902958 1108 10-11 -0.05762511105487533 1108 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-0.19040205912132535 2316 56-57 0.15240981133750608 2316 58-59 -0.1688891270359818 2316 60-61 0.0860047801735675 2316 62-63 0.10440980835804226 2316 64-65 0.1112165304001067 2316 66-67 0.27408895274225387 2316 68-69 0.3132747021624027 2316 70-71 0.36601137497562064 2316 72-73 0.3158972779520468 2316 74-75 0.4389585163915797 2316 76-77 0.34840286319049696 2316 78-79 0.38324519790737455 2316 80-81 0.3915564575218866 2316 82-83 0.5178878024738829 2316 84-85 0.61567085374379 2316 86-87 0.6091525581473114 2316 88-89 0.6682268649025893 2316 90-91 0.751023958150455 2316 92-93 0.6415090014454492 2316 94-95 1.005467007061604 2316 96-97 1.1779220548091125 2316 98-99 0.9977560138539339 2316 100-101 1.7444008622206546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 23.0 6 47.0 7 107.0 8 255.0 9 556.0 10 884.0 11 1382.0 12 2209.0 13 3156.0 14 4135.0 15 5583.0 16 7390.0 17 10229.0 18 9085.0 19 6101.0 20 5830.0 21 6034.0 22 6578.0 23 7319.0 24 8856.0 25 10600.0 26 12934.0 27 16439.0 28 20823.0 29 26180.0 30 33318.0 31 42663.0 32 55046.0 33 73536.0 34 103121.0 35 150180.0 36 235158.0 37 352836.0 38 261912.0 39 18833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91323657366137 19.685980345580425 15.220698527253026 23.180084553505182 2 15.374241672402478 20.568618976731283 39.87335862425901 24.183780726607225 3 18.908101633238893 24.163972169310473 30.308559964571362 26.619366232879273 4 12.938322688734866 17.125664953926766 37.62378680923492 32.31222554810345 5 13.798997964448548 37.21557637052906 34.90657272361053 14.078852941411867 6 32.255601086335965 36.64756508144852 17.414589769447065 13.68224406276845 7 28.586082520982398 32.48470005709133 21.06469995037856 17.864517471547707 8 26.146161808567967 35.46632145086677 20.06340072244543 18.324116018119827 9 25.87371077638873 16.91617445403081 20.416553284352172 36.79356148522829 10-11 24.5192256388093 26.86994114790923 28.129618661928617 20.48121455135285 12-13 25.3991207021843 23.802271046903332 28.61016184936014 22.188446401552227 14-15 22.273822291229813 25.576115548583655 26.3804630266942 25.76959913349234 16-17 22.12745906231492 28.917592399916764 27.090403054119378 21.864545483648936 18-19 21.92717253853665 27.590285670076142 29.177237900106178 21.30530389128103 20-21 22.414738915283653 26.642869695911664 29.485100381798652 21.45729100700603 22-23 22.18970419974929 26.566398070617097 29.217432075924098 22.02646565370952 24-25 22.23205240133898 26.767314658142087 29.278034296172144 21.72259864434679 26-27 22.115729323508862 27.12767642610721 28.996760192554277 21.759834057829647 28-29 21.873290859952565 27.167856561630423 29.242802602828533 21.71604997558848 30-31 22.070393264272205 26.96338675210825 29.115222083198834 21.850997900420715 32-33 22.038016473805317 26.94260846366759 29.02157601627372 21.997799046253377 34-35 22.379367886927152 26.854067623880184 28.94100251308565 21.82556197610701 36-37 22.346820493119697 26.781536290344093 28.944870850874242 21.926772365661968 38-39 22.12840017834377 27.03595521130302 29.04032176577674 21.79532284457647 40-41 22.348054359483296 26.786805233194073 29.05915355433547 21.805986852987157 42-43 22.186903644650418 26.85822742762693 28.805184860569994 22.149684067152656 44-45 22.22506789599774 27.03124416414558 28.588050037616252 22.155637902240436 46-47 22.124691199931704 27.15759874932638 28.403403603690126 22.314306447051795 48-49 22.4282223253779 27.221693104754586 28.296790880326967 22.05329368954055 50-51 22.469073306335 27.26447825460599 28.056387025861838 22.210061413197167 52-53 22.59841091245479 27.339395511791437 27.92174747607465 22.140446099679128 54-55 22.29556501742086 27.17297205726207 28.13308682684253 22.39837609847454 56-57 22.309871197690736 27.369590329689093 28.133987215810563 22.186551256809608 58-59 22.462103628767625 27.08360011524979 28.184142216104025 22.270154039878562 60-61 22.36709591877024 27.070427758124843 28.51038315218841 22.052093170916503 62-63 22.30910419968093 27.144059567066307 28.42541311179763 22.121423121455138 64-65 22.225434721132864 27.039681142253453 28.255439683276506 22.479444453337173 66-67 21.99596892524237 27.44105453555936 28.17683906114108 22.38613747805719 68-69 22.168109956834687 27.109511308885438 28.282584743275763 22.439793991004116 70-71 22.196222101281087 27.165268729424447 28.237965467748733 22.400543701545732 72-73 22.12983385433792 27.082756693413867 28.266071829936635 22.52133762231158 74-75 22.03795372934441 27.222493450503947 27.998195220335187 22.741357599816453 76-77 22.195588494229508 27.13845714682076 28.060188668171314 22.605765690778416 78-79 22.07930492639487 27.108710963136073 28.12028129485271 22.691702815616345 80-81 21.944413319887524 27.38849849801781 27.935768251884813 22.73131993020985 82-83 21.971350213559493 27.216296683625146 28.096312530513263 22.71604057230209 84-85 22.115757533517037 27.00611165041906 27.847876695860442 23.030254120203463 86-87 22.069267256788265 26.84946563582134 27.96578121748595 23.115485889904438 88-89 21.96115388514505 26.708104567839975 28.09200241170852 23.238739135306453 90-91 21.799166822027356 26.773955034003144 28.356799745464162 23.070078398505338 92-93 21.669809731997404 26.537209058030825 28.408975327354007 23.384005882617764 94-95 21.367497425554507 26.68986335430239 28.475234634695525 23.46740458544758 96-97 21.482847256681552 26.721843836537385 28.347079271578657 23.44822963520241 98-99 21.372380187602044 26.724887684213893 28.118530504007083 23.784201624176973 100-101 21.005278635019092 26.58391568858888 28.542850596318665 23.86795508007336 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 70.0 1 54.0 2 53.5 3 78.0 4 80.0 5 81.5 6 145.0 7 314.5 8 555.0 9 710.0 10 695.5 11 709.5 12 807.0 13 935.0 14 1040.5 15 1122.5 16 1218.5 17 1343.5 18 1450.0 19 1569.0 20 1772.5 21 2066.0 22 2468.0 23 2890.5 24 3579.0 25 4649.0 26 6181.0 27 8377.5 28 10497.0 29 13128.5 30 16442.5 31 19664.0 32 23222.5 33 26962.0 34 31155.0 35 34763.5 36 38353.0 37 42063.0 38 45285.0 39 47631.5 40 49542.0 41 52829.5 42 56069.5 43 58495.5 44 60046.5 45 61488.5 46 61229.0 47 60513.0 48 62274.0 49 62132.5 50 60873.5 51 59151.5 52 54836.0 53 50806.0 54 47207.5 55 42722.5 56 37583.0 57 32289.0 58 28147.0 59 24409.5 60 20203.0 61 16233.5 62 12569.0 63 9156.5 64 6472.0 65 4740.0 66 3563.0 67 2800.5 68 1981.0 69 1288.0 70 931.0 71 701.0 72 538.0 73 406.5 74 319.0 75 252.5 76 162.0 77 87.0 78 56.5 79 34.5 80 20.5 81 13.5 82 7.5 83 6.0 84 5.0 85 2.5 86 1.5 87 0.5 88 1.5 89 2.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04375223429855031 2 0.0 3 0.0 4 0.0 5 2.6678191645457505E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00583585442244383 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0004321867046565E-4 22-23 8.33693488920547E-4 24-25 6.669547911364376E-5 26-27 0.0023009940294207096 28-29 0.004001728746818626 30-31 2.6678191645457505E-4 32-33 1.3339095822728752E-4 34-35 0.0 36-37 0.0 38-39 3.334773955682188E-5 40-41 0.0 42-43 0.009404062555023771 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.669547911364376E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.334773955682188E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.6678191645457505E-4 84-85 1.667386977841094E-4 86-87 0.0 88-89 0.0 90-91 0.010171060564830673 92-93 0.0013005618427160534 94-95 0.0 96-97 0.0 98-99 5.335638329091501E-4 100-101 0.013038966166717354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1499352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.59606620582392 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3751734598133 44.75284720532506 2 13.498957840616274 15.819716546767543 3 4.498018704257007 7.9069860536903365 4 1.9644987580024107 4.604475971406726 5 1.0297846146229988 3.0170663728094054 6 0.6119324311613001 2.1514099949890997 7 0.4125994857605971 1.6923694748881797 8 0.2826348038287783 1.3249030141776919 9 0.20095781796065731 1.059780384522053 >10 1.024388972064686 10.727522395988277 >50 0.06749050293587679 2.751265370604994 >100 0.032535227064833694 3.3220938810258276 >500 6.849212742111546E-4 0.2934441804155251 >1k 3.424606371055773E-4 0.5761191533893134 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3775 0.2517754336540052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2856 0.19048228834856656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.13219043960324195 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.669547911364376E-5 0.0 8 0.0 0.0 0.0 6.669547911364376E-5 0.0 9 0.0 0.0 0.0 6.669547911364376E-5 0.0 10-11 0.0 0.0 0.0 6.669547911364376E-5 0.0 12-13 0.0 0.0 0.0 6.669547911364376E-5 0.0 14-15 0.0 0.0 0.0 6.669547911364376E-5 0.0 16-17 0.0 0.0 0.0 6.669547911364376E-5 0.0 18-19 0.0 0.0 0.0 6.669547911364376E-5 0.0 20-21 0.0 0.0 0.0 1.3339095822728752E-4 0.0 22-23 0.0 0.0 0.0 2.3343417689775315E-4 0.0 24-25 0.0 0.0 0.0 5.66911572465972E-4 0.0 26-27 0.0 0.0 0.0 7.336502702500814E-4 0.0 28-29 0.0 0.0 0.0 0.0013672573218296972 0.0 30-31 0.0 0.0 0.0 0.003034644299670791 0.0 32-33 0.0 0.0 0.0 0.005802506682887007 0.0 34-35 0.0 0.0 0.0 0.008036805233194073 0.0 36-37 0.0 0.0 0.0 0.012372011375580918 0.0 38-39 0.0 0.0 0.0 0.01824121353758157 0.0 40-41 0.0 0.0 0.0 0.026411409729002928 0.0 42-43 0.0 0.0 0.0 0.03878342110458385 0.0 44-45 0.0 0.0 0.0 0.050321738991244216 0.0 46-47 0.0 0.0 0.0 0.07149755360982611 0.0 48-49 0.0 0.0 0.0 0.09937626387932921 0.0 50-51 0.0 0.0 0.0 0.11528313564793324 0.0 52-53 0.0 0.0 0.0 0.14222810920984533 0.0 54-55 0.0 0.0 0.0 0.1833458720834067 0.0 56-57 0.0 0.0 0.0 0.256677551368858 0.0 58-59 0.0 0.0 0.0 0.35405295087477795 0.0 60-61 0.0 0.0 0.0 0.4638337094958355 0.0 62-63 0.0 0.0 0.0 0.5967911471088844 0.0 64-65 0.0 0.0 0.0 0.7525250908392426 0.0 66-67 0.0 0.0 0.0 0.9533785261899808 0.0 68-69 0.0 0.0 0.0 1.2078884744876452 0.0 70-71 0.0 0.0 0.0 1.3620884221983898 0.0 72-73 0.0 0.0 0.0 1.5492025888517174 0.0 74-75 0.0 0.0 0.0 1.781402899385868 0.0 76-77 0.0 0.0 0.0 2.0516529807543527 0.0 78-79 0.0 0.0 0.0 2.3289727829088833 0.0 80-81 0.0 0.0 0.0 2.7169070371733923 0.0 82-83 0.0 0.0 0.0 3.1326866539678475 0.0 84-85 0.0 0.0 0.0 3.6795895826997262 0.0 86-87 0.0 0.0 0.0 4.414873892188092 0.0 88-89 0.0 0.0 0.0 5.2168870285296585 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2395 0.0 44.245323 1 GTATCAA 4230 0.0 39.767746 1 ATCAACG 5215 0.0 31.512804 3 TCAACGC 5270 0.0 31.183924 4 TATCAAC 5350 0.0 30.71762 2 CAACGCA 5365 0.0 30.631739 5 AACGCAG 5525 0.0 29.744663 6 ACGCAGA 6290 0.0 26.128813 7 CGCAGAG 6315 0.0 25.950155 8 GTACATG 4330 0.0 23.265696 1 GCAGAGT 7065 0.0 22.590265 9 GAGTACT 3885 0.0 21.640957 12-13 TACATGG 4525 0.0 21.622896 2 ACATGGG 4360 0.0 21.242878 3 AGTACTT 4245 0.0 20.421112 12-13 AGAGTAC 6220 0.0 20.084433 10-11 TAGACTG 675 0.0 19.702417 5 GTACTTT 4315 0.0 19.483086 14-15 AGGATCG 220 1.3461486E-6 19.43055 5 CAGAGTA 6700 0.0 18.858234 10-11 >>END_MODULE