FastQCFastQC Report
Thu 26 May 2016
SRR937242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427128
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.19626830949991872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.1399313866730945No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18860.13215352792461502No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG502.816249E-547.5315935
GGTATCA24200.039.2905921
GTATCAA41600.037.2561721
ATCAACG47750.031.9498123
TCAACGC48100.031.6229614
TATCAAC49800.030.9209142
CAACGCA49450.030.7585645
AACGCAG50950.029.8362456
ACGCAGA58150.025.6518297
CGCAGAG58800.025.206688
GTACATG42250.023.9677241
GCAGAGT63350.023.4712439
TATGCCG1050.002188005322.6340925
TACATGG43200.022.3330962
GAGTACT34600.022.31004312-13
ACATGGG43050.021.0861283
CATGGGG23550.020.7894974
GTACTTT36800.020.71630314-15
GTCTAGG5800.020.4920751
CGCGGAA1552.6011548E-1019.91441782-83
Produced by FastQC (version 0.11.5)