##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1427128 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85220737032698 33.0 31.0 34.0 30.0 34.0 2 31.971682287783576 33.0 31.0 34.0 30.0 34.0 3 32.02257330807048 34.0 31.0 34.0 30.0 34.0 4 35.63184031145069 37.0 35.0 37.0 33.0 37.0 5 35.63523173814823 37.0 35.0 37.0 33.0 37.0 6 35.668699654130535 37.0 35.0 37.0 33.0 37.0 7 35.61095781177301 37.0 35.0 37.0 33.0 37.0 8 35.597583398265606 37.0 35.0 37.0 33.0 37.0 9 37.252094416198126 39.0 37.0 39.0 34.0 39.0 10-11 37.14284983547377 39.0 37.0 39.0 34.0 39.0 12-13 37.017312392441326 39.0 37.0 39.0 33.0 39.0 14-15 38.26024960620211 40.0 38.0 41.0 33.0 41.0 16-17 38.227717485747604 40.0 38.0 41.0 33.0 41.0 18-19 38.16549987106973 40.0 38.0 41.0 33.5 41.0 20-21 38.10008352439304 40.0 38.0 41.0 33.0 41.0 22-23 37.95465473314237 40.0 38.0 41.0 33.0 41.0 24-25 37.809869892539425 40.0 38.0 41.0 33.0 41.0 26-27 37.67285485254301 40.0 37.0 41.0 32.5 41.0 28-29 37.73734311147984 40.0 38.0 41.0 33.0 41.0 30-31 37.62038233431059 40.0 37.0 41.0 32.5 41.0 32-33 37.47557752352978 40.0 37.0 41.0 32.0 41.0 34-35 37.391215083720596 40.0 37.0 41.0 32.0 41.0 36-37 37.44866087695007 40.0 37.0 41.0 32.0 41.0 38-39 37.39186464003229 40.0 37.0 41.0 32.0 41.0 40-41 37.251544010067775 40.0 37.0 41.0 31.5 41.0 42-43 37.0729009591291 40.0 37.0 41.0 31.0 41.0 44-45 36.92280615333733 40.0 37.0 41.0 31.0 41.0 46-47 36.68705995537891 39.5 36.0 41.0 30.5 41.0 48-49 36.46487525996267 39.0 36.0 41.0 30.0 41.0 50-51 35.60684991115022 38.5 35.0 40.0 29.0 40.5 52-53 35.69545653928729 38.5 35.0 40.0 29.0 40.5 54-55 36.0482027540627 39.0 35.0 40.0 29.0 41.0 56-57 35.87384348145366 39.0 35.0 40.5 28.5 41.0 58-59 35.66256285350718 39.0 35.0 40.0 28.0 41.0 60-61 35.398344437219365 38.0 35.0 40.0 28.0 41.0 62-63 35.0844503786626 38.0 34.0 40.0 27.5 41.0 64-65 34.73455989932228 37.5 34.0 40.0 26.5 41.0 66-67 34.38319583106771 37.0 34.0 40.0 26.0 41.0 68-69 33.93227131693864 36.5 33.5 39.0 26.0 41.0 70-71 33.499190331911365 36.0 33.0 39.0 25.5 40.0 72-73 33.1095262653385 35.5 33.0 38.5 25.0 40.0 74-75 32.66043550403327 35.0 32.0 37.5 23.5 39.5 76-77 32.22669234995039 35.0 32.0 37.0 22.5 39.0 78-79 31.76519625429534 35.0 31.5 36.5 21.5 39.0 80-81 31.286276353627706 35.0 31.0 36.0 20.0 37.5 82-83 30.825459944728152 34.0 31.0 35.5 18.5 37.0 84-85 30.405880902063444 34.0 30.5 35.0 17.5 37.0 86-87 29.99613664646759 34.0 30.0 35.0 12.5 36.0 88-89 29.62664105812513 34.0 30.0 35.0 7.0 36.0 90-91 29.257381958731102 34.0 29.0 35.0 4.5 35.5 92-93 28.847544508971865 34.0 29.0 35.0 2.0 35.0 94-95 28.356185639970626 34.0 29.0 35.0 2.0 35.0 96-97 27.777528364659652 33.5 28.0 35.0 2.0 35.0 98-99 27.063361170126296 33.0 26.5 35.0 2.0 35.0 100-101 25.14592559321939 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13278159963761738 1101 2 0.15863519167254125 1101 3 0.10440612800213955 1101 4 0.04262092344524859 1101 5 0.11438316920962421 1101 6 0.04439547895707818 1101 7 -0.004493591305880784 1101 8 0.13226464210352162 1101 9 0.210420779830919 1101 10-11 0.21001631089608708 1101 12-13 0.12651899739542216 1101 14-15 0.15200731568797465 1101 16-17 0.3381499688613516 1101 18-19 0.19828332264722803 1101 20-21 0.14159342156396093 1101 22-23 0.18311440726616723 1101 24-25 0.14109669672899372 1101 26-27 0.1376919901187037 1101 28-29 0.14875870480111786 1101 30-31 0.09101105274571353 1101 32-33 -0.045588637212425454 1101 34-35 0.10067609099592545 1101 36-37 0.15534272981393826 1101 38-39 0.16252647989360014 1101 40-41 0.05908339371513449 1101 42-43 0.17007086450799136 1101 44-45 0.24408706988830886 1101 46-47 0.25527116293397256 1101 48-49 0.29939732896015414 1101 50-51 0.22812407131844736 1101 52-53 0.1325478893136136 1101 54-55 0.16362193075701725 1101 56-57 0.23775719394777894 1101 58-59 0.2860593614572835 1101 60-61 0.14031187576274817 1101 62-63 0.015597914398107093 1101 64-65 0.12523857856783138 1101 66-67 0.05613875834139748 1101 68-69 0.10431301076961574 1101 70-71 0.1335466245184378 1101 72-73 0.13165563782993672 1101 74-75 0.059455626975776 1101 76-77 -0.08347117225342515 1101 78-79 -0.02626890456799913 1101 80-81 0.10678416579397165 1101 82-83 0.2555318722361122 1101 84-85 0.3107582486981961 1101 86-87 0.23969258437533725 1101 88-89 0.15773539935458203 1101 90-91 0.3068270134127751 1101 92-93 0.4828464309260383 1101 94-95 0.43596567929903784 1101 96-97 0.2791221297798323 1101 98-99 0.41319286482701045 1101 100-101 0.9854267851312954 1102 1 0.031345160382997506 1102 2 0.08214472081013824 1102 3 0.08853678500877038 1102 4 0.07115255257777164 1102 5 0.04909413736420731 1102 6 -9.203854318400317E-4 1102 7 0.11757313480965337 1102 8 0.06923414354664459 1102 9 0.17083311168448034 1102 10-11 0.23222223435527667 1102 12-13 0.2774907205183794 1102 14-15 0.3506765834438923 1102 16-17 0.4701335994792899 1102 18-19 0.4780151059601394 1102 20-21 0.38540616810960415 1102 22-23 0.48171778183509417 1102 24-25 0.395125203184989 1102 26-27 0.29893334365176116 1102 28-29 0.3463773467731457 1102 30-31 0.4423207201140045 1102 32-33 0.3563721885629292 1102 34-35 0.28298735494486493 1102 36-37 0.3357316728082793 1102 38-39 0.37251026436970847 1102 40-41 0.4500695400350594 1102 42-43 0.4692371102799129 1102 44-45 0.45026437492140303 1102 46-47 0.4591134083611266 1102 48-49 0.3807036211150745 1102 50-51 0.24072211562798884 1102 52-53 0.32228411920833366 1102 54-55 0.3276084645417754 1102 56-57 0.3386657023048585 1102 58-59 0.4332456498745003 1102 60-61 0.47540843586450166 1102 62-63 0.28731225696051155 1102 64-65 0.4287802165371346 1102 66-67 0.3992533633487909 1102 68-69 0.4580072784651179 1102 70-71 0.5099525942156262 1102 72-73 0.37880654289460836 1102 74-75 0.44361512232095635 1102 76-77 0.6120433987601714 1102 78-79 0.44071945457081085 1102 80-81 0.4891109565299381 1102 82-83 0.5597170361192347 1102 84-85 0.5583381039104047 1102 86-87 0.43584927160744513 1102 88-89 0.32668899460124834 1102 90-91 0.5312061030395903 1102 92-93 0.5988874143939391 1102 94-95 0.5445496765988693 1102 96-97 0.6849987870780616 1102 98-99 0.9204256087465872 1102 100-101 1.4451383977180186 1103 1 -0.04534504382935722 1103 2 -0.005229732903593742 1103 3 -0.019599088820495325 1103 4 -0.0800558436663863 1103 5 -0.06965777961553243 1103 6 -0.07684591595863566 1103 7 0.009996891971319144 1103 8 0.07879531828877617 1103 9 0.020536392875960985 1103 10-11 0.1043268535774331 1103 12-13 -0.02940693267478167 1103 14-15 0.05898195640977377 1103 16-17 -0.022422799970989615 1103 18-19 9.491737191282823E-4 1103 20-21 -0.13409470544005586 1103 22-23 -0.27622147977395883 1103 24-25 -0.21100557338455417 1103 26-27 0.08473489960384484 1103 28-29 0.011920303217671346 1103 30-31 -0.192413724177932 1103 32-33 -0.12799012865676218 1103 34-35 -0.0620617959919727 1103 36-37 -0.04664689870502059 1103 38-39 -0.08744710030634906 1103 40-41 -0.05269069896677081 1103 42-43 0.0745729227658245 1103 44-45 0.1042029315902937 1103 46-47 0.13504957230038883 1103 48-49 0.12336593104812721 1103 50-51 0.17870940975919325 1103 52-53 0.13030251713011864 1103 54-55 0.07015427294378895 1103 56-57 0.03603518246231374 1103 58-59 0.07534405003310951 1103 60-61 0.02812283680939487 1103 62-63 -0.028031121770290213 1103 64-65 0.09487539023879776 1103 66-67 0.20775111211556663 1103 68-69 0.219065391970652 1103 70-71 0.37082688948726883 1103 72-73 0.3248661222012217 1103 74-75 0.2669797373744842 1103 76-77 0.3401836869383672 1103 78-79 0.31729137795602114 1103 80-81 0.19779633630798443 1103 82-83 0.13529878121896743 1103 84-85 0.18031965515158888 1103 86-87 0.29856429106955673 1103 88-89 0.3135404199868894 1103 90-91 0.17559831243393376 1103 92-93 0.12341437581371295 1103 94-95 0.12363049222820521 1103 96-97 0.3084366074572742 1103 98-99 0.6004386908094226 1103 100-101 0.858973229896506 1104 1 0.08261791986386058 1104 2 0.013629304532749131 1104 3 0.025339330783232583 1104 4 0.03435435025632927 1104 5 0.08289046007765677 1104 6 -0.015688338583871086 1104 7 0.06391616028869151 1104 8 0.09488500146377987 1104 9 0.07111999249757162 1104 10-11 0.07918194499613662 1104 12-13 0.1132837115478722 1104 14-15 0.23834009102917975 1104 16-17 0.21471759036315063 1104 18-19 0.23507758184759098 1104 20-21 0.20320885355588558 1104 22-23 0.2594880957815846 1104 24-25 0.18731162929817913 1104 26-27 0.023563958522892392 1104 28-29 0.07177349523747978 1104 30-31 0.15829242720175074 1104 32-33 0.02309084181996468 1104 34-35 0.19350221594159223 1104 36-37 0.22522390185984875 1104 38-39 0.19233063277968654 1104 40-41 0.15374033269820586 1104 42-43 0.2997380083526977 1104 44-45 0.21018139367745903 1104 46-47 0.13197208602667132 1104 48-49 0.11529085681383577 1104 50-51 0.11986276770803528 1104 52-53 0.21283304224490251 1104 54-55 0.2926742210137334 1104 56-57 0.31072846349755423 1104 58-59 0.18109883434946994 1104 60-61 0.16866345787558856 1104 62-63 0.018133144029881976 1104 64-65 0.08154158808035561 1104 66-67 0.1562776211080319 1104 68-69 0.24703267148373698 1104 70-71 0.24627561426108713 1104 72-73 0.2923678890584469 1104 74-75 0.23303877165611908 1104 76-77 0.17392472284905125 1104 78-79 0.23512572583563696 1104 80-81 0.2900131385615019 1104 82-83 0.3487068724391911 1104 84-85 0.4532251570989665 1104 86-87 0.49626159304681394 1104 88-89 0.10040957090081193 1104 90-91 0.1176453956188439 1104 92-93 0.4541210836493583 1104 94-95 0.4727830579940715 1104 96-97 0.5197044244542433 1104 98-99 0.7643004031777743 1104 100-101 1.0147838569785606 1105 1 0.1987491420683547 1105 2 0.14112193805243933 1105 3 0.042992919217915926 1105 4 0.06401417012592958 1105 5 0.06845072943953312 1105 6 0.12870134524703047 1105 7 0.2703631704624101 1105 8 0.13308160132991276 1105 9 0.1721518952528882 1105 10-11 0.18010379718059255 1105 12-13 0.12913885450642226 1105 14-15 0.10757428674292413 1105 16-17 0.07268756227881568 1105 18-19 0.060504027809663796 1105 20-21 0.11080829806252979 1105 22-23 0.13417086667391942 1105 24-25 0.18834841524981272 1105 26-27 0.13263026756489893 1105 28-29 0.1877214189262233 1105 30-31 0.05788747921374693 1105 32-33 -0.0739287409883218 1105 34-35 -0.0016147068348217886 1105 36-37 -0.0548680213334336 1105 38-39 0.2033231841187586 1105 40-41 0.16759443708426858 1105 42-43 0.14407957483443568 1105 44-45 0.16343217320233805 1105 46-47 0.07879681739131428 1105 48-49 0.15293339255049432 1105 50-51 0.0836850717668085 1105 52-53 0.14456548119899537 1105 54-55 0.11354059532383332 1105 56-57 0.1490890672988172 1105 58-59 0.1831203585260539 1105 60-61 0.2553325701105038 1105 62-63 0.13935146578249658 1105 64-65 0.300423836351591 1105 66-67 0.27350184557308665 1105 68-69 0.2910729090214659 1105 70-71 0.33107017461858135 1105 72-73 0.31678387068809855 1105 74-75 0.4113521394656061 1105 76-77 0.3918804739441981 1105 78-79 0.20435456194365997 1105 80-81 0.32372660689337707 1105 82-83 0.5362168472609525 1105 84-85 0.6221793431892415 1105 86-87 0.5276776961608611 1105 88-89 0.27861765223342516 1105 90-91 0.5467981967869981 1105 92-93 0.7299714148729919 1105 94-95 0.5064910476178746 1105 96-97 0.40294124518377217 1105 98-99 0.6228457585434519 1105 100-101 0.9569003533685674 1106 1 -0.0960306828178652 1106 2 -0.17730262102878314 1106 3 -0.099789541413557 1106 4 -0.04861845538540166 1106 5 -0.16635247893887595 1106 6 -0.15918909638607914 1106 7 -0.07581135435994923 1106 8 -0.05581211457523949 1106 9 0.010680717885307445 1106 10-11 -0.2704109603687499 1106 12-13 -0.23764259147975508 1106 14-15 -0.33383898586718885 1106 16-17 -0.36458170067466966 1106 18-19 -0.25807214493661235 1106 20-21 -0.18564044339827745 1106 22-23 -0.14168859328986372 1106 24-25 -0.10062504361185631 1106 26-27 -0.17407484677220708 1106 28-29 -0.08121279532137748 1106 30-31 -0.20346911321082217 1106 32-33 -0.03442463429895781 1106 34-35 -0.07225078447267919 1106 36-37 -0.07875869038704053 1106 38-39 -0.2131457044656031 1106 40-41 -0.350105146269712 1106 42-43 -0.2978334397408844 1106 44-45 -0.04832794482307179 1106 46-47 -0.03553198599782803 1106 48-49 0.15893058769768942 1106 50-51 0.06898773054567187 1106 52-53 -0.09687140815510986 1106 54-55 0.03162854291215922 1106 56-57 -0.243745291856456 1106 58-59 -0.14395303123477277 1106 60-61 -0.24051496485540014 1106 62-63 -0.2826237687084543 1106 64-65 0.014897984763877048 1106 66-67 0.034042494071378826 1106 68-69 -0.1449152184623017 1106 70-71 -0.12956085894926872 1106 72-73 0.13995422905032484 1106 74-75 -0.18543044362305494 1106 76-77 0.002815463754011205 1106 78-79 0.10249335842272345 1106 80-81 0.025800944239609436 1106 82-83 -0.01245697051691863 1106 84-85 -0.1598604145787732 1106 86-87 0.054502526980424904 1106 88-89 0.09165030456871293 1106 90-91 0.002523070466576627 1106 92-93 0.12869523600385335 1106 94-95 0.21462789061317622 1106 96-97 0.3554001564227143 1106 98-99 0.549131340375471 1106 100-101 0.5703726853325151 1107 1 -0.005537785185829591 1107 2 -0.08008301958529529 1107 3 -0.08356306189468299 1107 4 -0.13815128400858612 1107 5 -0.18619079474697742 1107 6 -0.13636551200403346 1107 7 -0.08977659142925631 1107 8 -0.03132309730850835 1107 9 0.01325887296306405 1107 10-11 -0.06508700101373677 1107 12-13 -0.046313936150056634 1107 14-15 -0.058546282322538445 1107 16-17 -0.008246973202254537 1107 18-19 -0.04490105566264191 1107 20-21 -0.1797098227264442 1107 22-23 -0.029415296410121528 1107 24-25 0.06728456336809785 1107 26-27 0.11506351721214259 1107 28-29 0.0156197372632505 1107 30-31 0.1607920587369236 1107 32-33 0.17327357778851393 1107 34-35 0.1455640943160077 1107 36-37 0.14576664914493165 1107 38-39 0.16020346413121445 1107 40-41 0.19537699983357015 1107 42-43 0.2581339524725337 1107 44-45 0.3335678424162367 1107 46-47 0.2584278044596786 1107 48-49 0.23794136410943878 1107 50-51 0.21754251219391563 1107 52-53 0.1537859177105645 1107 54-55 0.196081659542223 1107 56-57 0.33694664211236613 1107 58-59 0.22275427766207656 1107 60-61 0.43298502235750647 1107 62-63 0.4230958994353031 1107 64-65 0.4593935199937107 1107 66-67 0.5911498386338607 1107 68-69 0.5289970662047239 1107 70-71 0.3933622574250606 1107 72-73 0.512876585110817 1107 74-75 0.38354056146344107 1107 76-77 0.38260414489290184 1107 78-79 0.46010385794200204 1107 80-81 0.43243820749689377 1107 82-83 0.3314134485058595 1107 84-85 0.41478961642129875 1107 86-87 0.28054745738060305 1107 88-89 0.3731737799494077 1107 90-91 0.5470358164367397 1107 92-93 0.5975571014960437 1107 94-95 0.6503877655346422 1107 96-97 0.7281732144947952 1107 98-99 0.8155064266883691 1107 100-101 0.7313107328880726 1108 1 0.026029312364730828 1108 2 1.4600879093151775E-4 1108 3 -0.10134963850273238 1108 4 0.03967488733569269 1108 5 -0.09144447278742263 1108 6 0.022829395025972588 1108 7 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-1.295756300079951 2316 72-73 -1.0871320103851438 2316 74-75 -1.4897154351757713 2316 76-77 -1.6105060812426402 2316 78-79 -1.4257274631344998 2316 80-81 -1.7362196541941763 2316 82-83 -1.6433786941862039 2316 84-85 -1.7548000881099561 2316 86-87 -1.9108318294095419 2316 88-89 -1.8311052970195583 2316 90-91 -2.0056150117478353 2316 92-93 -2.009504354592355 2316 94-95 -2.268614485232977 2316 96-97 -2.2991823783268863 2316 98-99 -2.5278918908713734 2316 100-101 -2.5879430642149615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4736.0 3 944.0 4 731.0 5 923.0 6 990.0 7 1117.0 8 1385.0 9 1804.0 10 2034.0 11 2663.0 12 3367.0 13 4314.0 14 4988.0 15 5936.0 16 7298.0 17 9111.0 18 8173.0 19 6441.0 20 6642.0 21 7075.0 22 7763.0 23 8674.0 24 10158.0 25 11789.0 26 13678.0 27 16486.0 28 19780.0 29 24208.0 30 30261.0 31 38179.0 32 49587.0 33 66004.0 34 91710.0 35 132501.0 36 204679.0 37 309103.0 38 275572.0 39 36321.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.716031856247696 19.29429640478687 14.605043812179701 23.38462792678573 2 14.957574190720136 20.276572157597233 40.66934283903765 24.096510812644983 3 18.700509168975536 24.24067226040677 29.996361071615237 27.062457499002456 4 12.589502495391395 16.659126163391935 37.883652376242075 32.86771896497459 5 13.7943098735223 37.300769348038834 34.88348076067874 14.021440017760131 6 32.107787037752146 36.50246789628517 17.746245497756778 13.643499568205913 7 28.556776371627922 32.36531534472434 21.14089790004191 17.937010383605823 8 25.871687293416574 35.71039425315439 20.155873077387522 18.262045376041517 9 25.828900618977045 17.162028492460582 20.477981981779596 36.53108890678277 10-11 24.53761810789338 26.950805536821505 28.108844807992277 20.402731547292834 12-13 25.35791728874867 23.97757099633403 28.426792780958444 22.23771893395885 14-15 22.24605335396462 25.785502964811208 26.164371155328585 25.804072525895588 16-17 22.276292626077005 29.218132131404772 26.6975924570558 21.80798278546242 18-19 22.07174145248518 27.988756239756903 28.659899770751128 21.27960253700679 20-21 22.613672779500973 27.196666377679723 28.693174140138073 21.496486702681224 22-23 22.355429193211535 26.991523192755224 28.592709594762983 22.060338019270258 24-25 22.263376276592684 27.223494710419043 28.59964214340517 21.913486869583103 26-27 22.177757452362894 27.479321366686825 28.39319288908419 21.949728291866094 28-29 22.016104036329033 27.620574048976238 28.596827282297323 21.76649463239741 30-31 22.171846581424763 27.36376724137023 28.554598242855523 21.90978793434948 32-33 22.07007356562477 27.410768726904333 28.40376600395472 22.115391703516174 34-35 22.469112312035683 27.405630802521706 28.2119720025585 21.91328488288411 36-37 22.38858775864919 27.273987647244844 28.21948551129294 22.11793908281302 38-39 22.194739627865314 27.499512122526742 28.422478024454247 21.883270225153698 40-41 22.417848161465983 27.28878312616592 28.403503507192507 21.88986520517559 42-43 22.231964472998833 27.364945026093473 28.12928660984181 22.27380389106588 44-45 22.372418382830674 27.572139963586466 27.938240911271944 22.117200742310914 46-47 22.2201901975128 27.643578341689985 27.75811667113634 22.37811478966087 48-49 22.388457377234484 27.696811322728514 27.71232748797497 22.202403812062027 50-51 22.397825730563447 27.72428735770587 27.660045025800496 22.21784188593019 52-53 22.582703075061154 27.71891154007256 27.512533482013545 22.185851902852743 54-55 22.287526634862797 27.609374067560406 27.649427284630583 22.45367201294621 56-57 22.25456247995064 27.943140889886276 27.644637932232634 22.15765869793045 58-59 22.4032492201254 27.565267993272442 27.72033036920148 22.311152417400677 60-61 22.433469017019775 27.518799525773936 27.991516435414777 22.056215021791516 62-63 22.38131122833459 27.60143752222217 27.951200901066276 22.06605034837696 64-65 22.245690213391427 27.520829012052555 27.797812142123373 22.435668632432645 66-67 22.094609913446682 27.761286640915696 27.65252468815607 22.49157875748155 68-69 22.1218607501245 27.57777429162337 27.901600601115966 22.398764357136162 70-71 22.119799331784527 27.617677419490704 27.790820969989973 22.471702278734792 72-73 22.140553471719397 27.500307983457688 27.813591213468865 22.545547331354047 74-75 22.01162343387601 27.68483913358455 27.680978227296464 22.622559205242972 76-77 22.14372083472999 27.58104851942655 27.719409701077367 22.55582094476609 78-79 22.138293832275345 27.592781214704036 27.59966052699744 22.669264426023176 80-81 22.031386613568245 27.832269645064017 27.558880173700068 22.577463567667667 82-83 22.13876131091892 27.574495356302283 27.754683883901592 22.53205944887721 84-85 22.060429137916334 27.40575429030876 27.596461975856563 22.93735459591835 86-87 22.214758646926327 27.266218711768186 27.712875088527593 22.80614755277789 88-89 22.211748289641125 26.981170084108875 27.82801443952099 22.979067186729004 90-91 21.926091636655645 27.13483041564548 28.090948048789304 22.848129898909566 92-93 21.980620632509034 27.001680050566467 28.13872367397262 22.878975642951875 94-95 21.732981116236203 27.18915244339205 27.99932897659455 23.078537463777195 96-97 21.78254271740534 27.298499626591198 27.92150273178018 22.99745492422328 98-99 21.71789050152901 27.228262262010013 27.761670095081932 23.292177141379046 100-101 21.710439733406425 27.24649236988016 28.038685618286596 23.004382278426817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1715.0 1 938.5 2 154.0 3 141.0 4 120.5 5 130.5 6 189.5 7 298.0 8 465.0 9 604.0 10 648.5 11 672.5 12 721.0 13 822.5 14 918.0 15 962.0 16 1018.5 17 1119.5 18 1244.5 19 1335.0 20 1447.0 21 1673.5 22 2081.0 23 2570.0 24 3169.5 25 4148.5 26 5543.5 27 7609.0 28 9788.5 29 12379.5 30 15788.5 31 19173.5 32 22514.5 33 26079.0 34 30282.0 35 33336.0 36 36533.5 37 40532.0 38 43714.0 39 45830.0 40 47419.0 41 50360.0 42 53560.0 43 55832.0 44 57336.5 45 58599.0 46 58101.0 47 57417.5 48 59605.0 49 59495.5 50 57444.0 51 55649.5 52 51801.5 53 47929.0 54 44637.5 55 40536.5 56 35714.0 57 30740.0 58 26587.0 59 23007.5 60 18865.5 61 14935.5 62 11463.5 63 8449.5 64 6275.5 65 4665.5 66 3447.0 67 2666.0 68 1885.0 69 1256.0 70 995.5 71 775.0 72 592.0 73 441.5 74 315.0 75 227.5 76 163.0 77 103.0 78 61.0 79 44.5 80 26.0 81 16.5 82 12.5 83 13.0 84 14.0 85 11.0 86 10.5 87 7.5 88 5.5 89 5.5 90 4.5 91 4.5 92 5.0 93 5.0 94 4.5 95 4.0 96 5.5 97 7.0 98 7.0 99 4.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29681990683386494 2 0.25940209988172047 3 0.2544971439142109 4 0.2595422414807922 5 0.26031302027568654 6 0.19886092908274522 7 0.18715910556025808 8 0.19367568991709225 9 0.1878598135556166 10-11 0.20695410642913598 12-13 0.19700405289504516 14-15 0.1929749819217337 16-17 0.19272973412335823 18-19 0.18873569854981473 20-21 0.18298989298787494 22-23 0.18589783116861278 24-25 0.17762947682338234 26-27 0.17724408742593517 28-29 0.18936633574563738 30-31 0.1981952564871546 32-33 0.1929399465219658 34-35 0.20005213267485467 36-37 0.18130819379901453 38-39 0.18148337079785415 40-41 0.1790659282138673 42-43 0.18456648597743158 44-45 0.1862131497665241 46-47 0.19017214994029968 48-49 0.1966887342971338 50-51 0.19854561048483388 52-53 0.21319040758782673 54-55 0.1943063271129149 56-57 0.17650834403080873 58-59 0.1779798308210616 60-61 0.1745463616438049 62-63 0.18172861859622963 64-65 0.17465146784310867 66-67 0.17216395445958596 68-69 0.17128806946538783 70-71 0.18130819379901453 72-73 0.17787472462175782 74-75 0.18162351239692584 76-77 0.18186876019530132 78-79 0.17987174240852957 80-81 0.18242932659158814 82-83 0.17580763603545022 84-85 0.17058736147002931 86-87 0.17034211367165383 88-89 0.17006183047351045 90-91 0.1760528838338257 92-93 0.17296976865424823 94-95 0.17086764466817272 96-97 0.16929105167861608 98-99 0.1761930254328974 100-101 0.1690458038802406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1427128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.92133019908427 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8569259620395 47.431474948190655 2 12.94628302629045 15.77409566190881 3 4.15683518361 7.5971978641162785 4 1.756023332851463 4.279171091917617 5 0.9219935778004729 2.808453759730885 6 0.5332887394249382 1.949319563157604 7 0.36608156548516213 1.561152315150348 8 0.2576594892243391 1.2557567057570689 9 0.18741980152913357 1.0276077253322868 >10 0.9239380475923323 10.029778370541182 >50 0.06523241282544195 2.7477273248995306 >100 0.027397953564424725 2.8185342589539735 >500 4.604538812071649E-4 0.15814012231563374 >1k 4.604538812071649E-4 0.5615902880282018 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2801 0.19626830949991872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.1399313866730945 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1886 0.13215352792461502 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.0510619930377652E-4 7.007079953585102E-5 22-23 0.0 0.0 0.0 1.4014159907170203E-4 7.007079953585102E-5 24-25 0.0 0.0 0.0 2.1021239860755306E-4 7.007079953585102E-5 26-27 0.0 0.0 0.0 4.5546019698303165E-4 7.007079953585102E-5 28-29 0.0 0.0 0.0 0.0011211327925736165 7.007079953585102E-5 30-31 0.0 0.0 0.0 0.0029079381807378176 7.007079953585102E-5 32-33 0.0 0.0 0.0 0.005500557763564305 7.007079953585102E-5 34-35 0.0 0.0 0.0 0.008934026940821006 7.007079953585102E-5 36-37 0.0 0.0 0.0 0.01296309791413244 7.007079953585102E-5 38-39 0.0 0.0 0.0 0.01888408047491185 7.007079953585102E-5 40-41 0.0 0.0 0.0 0.02561087723035355 7.007079953585102E-5 42-43 0.0 0.0 0.0 0.03591128476212365 7.007079953585102E-5 44-45 0.0 0.0 0.0 0.04561609049783902 7.007079953585102E-5 46-47 0.0 0.0 0.0 0.06555123296578863 7.007079953585102E-5 48-49 0.0 0.0 0.0 0.09004097740356856 7.007079953585102E-5 50-51 0.0 0.0 0.0 0.10373981871282745 7.007079953585102E-5 52-53 0.0 0.0 0.0 0.12731864275664131 7.007079953585102E-5 54-55 0.0 0.0 0.0 0.16473644970878576 7.007079953585102E-5 56-57 0.0 0.0 0.0 0.22758995689244413 7.007079953585102E-5 58-59 0.0 0.0 0.0 0.3124106597305918 7.007079953585102E-5 60-61 0.0 0.0 0.0 0.41537969964852484 7.007079953585102E-5 62-63 0.0 0.0 0.0 0.5352007668548302 7.007079953585102E-5 64-65 0.0 0.0 0.0 0.6789159767028605 7.007079953585102E-5 66-67 0.0 0.0 0.0 0.8669159318575489 7.007079953585102E-5 68-69 0.0 0.0 0.0 1.0956270215425667 7.007079953585102E-5 70-71 0.0 0.0 0.0 1.2340168506258724 7.007079953585102E-5 72-73 0.0 0.0 0.0 1.4012057783184129 7.007079953585102E-5 74-75 0.0 0.0 0.0 1.6303372928006459 7.007079953585102E-5 76-77 0.0 0.0 0.0 1.888443082890953 7.007079953585102E-5 78-79 0.0 0.0 0.0 2.1406979612200168 7.007079953585102E-5 80-81 0.0 0.0 0.0 2.5264377126648765 7.007079953585102E-5 82-83 0.0 0.0 0.0 2.9412568459171147 7.007079953585102E-5 84-85 0.0 0.0 0.0 3.493800135657068 7.007079953585102E-5 86-87 0.0 0.0 0.0 4.26794232892915 7.007079953585102E-5 88-89 0.0 0.0 0.0 5.0872451525020885 7.007079953585102E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 50 2.816249E-5 47.531593 5 GGTATCA 2420 0.0 39.290592 1 GTATCAA 4160 0.0 37.256172 1 ATCAACG 4775 0.0 31.949812 3 TCAACGC 4810 0.0 31.622961 4 TATCAAC 4980 0.0 30.920914 2 CAACGCA 4945 0.0 30.758564 5 AACGCAG 5095 0.0 29.836245 6 ACGCAGA 5815 0.0 25.651829 7 CGCAGAG 5880 0.0 25.20668 8 GTACATG 4225 0.0 23.967724 1 GCAGAGT 6335 0.0 23.471243 9 TATGCCG 105 0.0021880053 22.634092 5 TACATGG 4320 0.0 22.333096 2 GAGTACT 3460 0.0 22.310043 12-13 ACATGGG 4305 0.0 21.086128 3 CATGGGG 2355 0.0 20.789497 4 GTACTTT 3680 0.0 20.716303 14-15 GTCTAGG 580 0.0 20.492075 1 CGCGGAA 155 2.6011548E-10 19.914417 82-83 >>END_MODULE