FastQCFastQC Report
Thu 26 May 2016
SRR937242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427128
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38230.2678806666255585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.2107729650038399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.14147294426288323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.043.6520651
GTATCAA42050.041.3621141
ATCAACG51600.033.1415633
TCAACGC52450.032.6044734
TATCAAC53400.032.380262
CAACGCA53650.031.8752045
AACGCAG54550.031.3460126
GTACCGT1205.3847107E-627.7071176
ACGCAGA62700.027.047117
CGCAGAG64400.026.1118498
GTACATG38700.023.4536291
GCAGAGT70350.023.4307169
TACATGG40700.021.2420862
GAGTACT41800.021.19447912-13
GTACTTT43800.020.17034714-15
AGAGTAC63350.020.13343410-11
AGTACTT43550.020.01557512-13
GTATAGA4750.020.0089361
ACATGGG39750.019.718193
CAGCGTA1456.144501E-419.6563769
Produced by FastQC (version 0.11.5)