##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937242_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1427128 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25042883329316 33.0 31.0 34.0 31.0 34.0 2 32.484463201618915 34.0 31.0 34.0 31.0 34.0 3 32.57713533754506 34.0 31.0 34.0 31.0 34.0 4 36.17069527050131 37.0 35.0 37.0 35.0 37.0 5 36.02565502183406 37.0 35.0 37.0 35.0 37.0 6 36.10146602126789 37.0 35.0 37.0 35.0 37.0 7 36.068771686912456 37.0 35.0 37.0 35.0 37.0 8 36.041794429091155 37.0 35.0 37.0 35.0 37.0 9 37.77171774360814 39.0 37.0 39.0 35.0 39.0 10-11 37.691202541047474 39.0 37.0 39.0 35.0 39.0 12-13 37.61095395787905 39.0 37.0 39.0 35.0 39.0 14-15 38.89425405429646 40.0 38.0 41.0 35.0 41.0 16-17 38.86562838091608 40.0 38.0 41.0 35.0 41.0 18-19 38.84217673537342 40.0 38.0 41.0 35.0 41.0 20-21 38.750244547090375 40.0 38.0 41.0 34.5 41.0 22-23 38.622447320772906 40.0 38.0 41.0 34.0 41.0 24-25 38.4893867263483 40.0 38.0 41.0 34.0 41.0 26-27 38.29096338940866 40.0 38.0 41.0 34.0 41.0 28-29 38.28740309208425 40.0 38.0 41.0 34.0 41.0 30-31 38.4551487322791 40.0 38.0 41.0 34.0 41.0 32-33 38.46040544366028 40.0 38.0 41.0 34.0 41.0 34-35 38.3659121676542 40.0 38.0 41.0 34.0 41.0 36-37 38.24671367950177 40.0 38.0 41.0 34.0 41.0 38-39 38.125369273113556 40.0 38.0 41.0 34.0 41.0 40-41 37.99811124159851 40.0 38.0 41.0 33.0 41.0 42-43 37.832767978765744 40.0 37.5 41.0 33.0 41.0 44-45 37.618058085889984 40.0 37.0 41.0 33.0 41.0 46-47 37.36905414230539 40.0 37.0 41.0 32.5 41.0 48-49 37.131976949509784 40.0 37.0 41.0 32.0 41.0 50-51 36.961793546199075 40.0 36.5 41.0 31.0 41.0 52-53 36.7519784490249 39.0 36.0 41.0 31.0 41.0 54-55 36.56185569899827 39.0 36.0 41.0 31.0 41.0 56-57 36.343681856147455 39.0 35.5 40.5 30.5 41.0 58-59 36.10683064168036 39.0 35.0 40.0 30.0 41.0 60-61 35.79695339170698 38.0 35.0 40.0 29.0 41.0 62-63 35.45275371235096 38.0 34.5 40.0 28.5 41.0 64-65 35.1252869399241 37.5 34.0 40.0 28.0 41.0 66-67 34.75440605187481 37.0 34.0 39.5 28.0 41.0 68-69 34.33009127422348 36.5 34.0 39.0 27.5 40.5 70-71 33.9584094769355 36.0 33.5 39.0 26.5 40.0 72-73 33.52441827222225 35.5 33.0 38.5 26.0 40.0 74-75 33.09717558621231 35.0 33.0 37.5 26.0 39.5 76-77 31.73195676911952 34.0 30.5 36.0 25.0 39.0 78-79 32.36091261610731 35.0 32.0 36.0 26.0 39.0 80-81 32.19122005874736 35.0 32.0 36.0 25.5 38.0 82-83 31.863470200290372 35.0 32.0 36.0 25.0 37.0 84-85 31.533333730401196 34.5 32.0 35.0 24.5 37.0 86-87 31.210305943124936 34.0 31.5 35.0 24.0 36.0 88-89 30.87313541602435 34.0 31.0 35.0 22.5 36.0 90-91 30.463415685208336 34.0 31.0 35.0 20.0 35.5 92-93 30.102599766804378 34.0 31.0 35.0 18.5 35.0 94-95 29.74372726202555 34.0 30.0 35.0 14.0 35.0 96-97 29.3659118173002 34.0 30.0 35.0 4.5 35.0 98-99 28.764596448251314 34.0 29.5 35.0 2.0 35.0 100-101 27.188442452253756 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09915878005666912 1101 2 0.16058219602321344 1101 3 0.11579052812220425 1101 4 0.07956247025713026 1101 5 0.0048921808244699605 1101 6 0.06318844985818828 1101 7 0.10443264165836297 1101 8 0.06362240738003777 1101 9 0.09046363815379976 1101 10-11 0.1512082370659371 1101 12-13 0.1084328193043973 1101 14-15 0.1930892150050667 1101 16-17 0.19979601565049165 1101 18-19 0.2289428235685591 1101 20-21 0.1242969929223321 1101 22-23 0.0101728299752466 1101 24-25 0.07976609802601331 1101 26-27 0.11140233920745146 1101 28-29 0.09364815477429289 1101 30-31 0.25252456641892707 1101 32-33 0.2613285849828202 1101 34-35 0.2791210742679908 1101 36-37 0.2500351495940194 1101 38-39 0.21259981859898858 1101 40-41 0.27226558083575014 1101 42-43 0.29305647560361336 1101 44-45 0.3322858128768047 1101 46-47 0.21771104891634252 1101 48-49 0.3192944598949907 1101 50-51 0.23460340577030792 1101 52-53 0.3444448009172518 1101 54-55 0.21550798799296444 1101 56-57 0.17329378861894895 1101 58-59 0.2977030615685834 1101 60-61 0.3402602109488697 1101 62-63 0.1639794114803621 1101 64-65 0.20090533095500263 1101 66-67 0.21612082018897638 1101 68-69 0.023255847841689103 1101 70-71 0.07013172910037468 1101 72-73 0.039458307713509555 1101 74-75 0.09302620688424668 1101 76-77 0.07193892493826226 1101 78-79 0.06503375482266449 1101 80-81 0.016421508756728542 1101 82-83 0.15045576028181173 1101 84-85 0.13821841961686587 1101 86-87 0.034974065262552756 1101 88-89 0.15673722184836336 1101 90-91 0.2507555995444797 1101 92-93 0.3670180420235454 1101 94-95 0.05385177710655498 1101 96-97 0.14628410513848777 1101 98-99 0.1519198765765566 1101 100-101 0.17744231854848636 1102 1 0.14242313498412074 1102 2 0.15134607366810116 1102 3 0.12627201560048462 1102 4 0.0879191441172722 1102 5 -0.0021699899613238927 1102 6 0.010508941269918637 1102 7 0.12044255141369575 1102 8 0.18943187457159638 1102 9 0.20384261339390974 1102 10-11 0.17239807227657167 1102 12-13 0.11134937823006652 1102 14-15 0.19879566166259366 1102 16-17 0.18193839810002288 1102 18-19 0.14516271751114118 1102 20-21 0.12799905421910296 1102 22-23 0.23679746355514908 1102 24-25 0.1442292497986415 1102 26-27 0.10786511157066059 1102 28-29 0.09788308090222841 1102 30-31 0.1312841231462727 1102 32-33 0.18716662871237588 1102 34-35 0.16739325501831814 1102 36-37 0.17416947583618736 1102 38-39 0.24670054978781764 1102 40-41 0.1072760488304283 1102 42-43 0.33861621728298275 1102 44-45 0.290384230554217 1102 46-47 0.28121178155513604 1102 48-49 0.3804611398530753 1102 50-51 0.21092686801424776 1102 52-53 0.2943971934924221 1102 54-55 0.38913150106648686 1102 56-57 0.43838880611125575 1102 58-59 0.3033674187515487 1102 60-61 0.3480131324417357 1102 62-63 0.274529430289725 1102 64-65 0.27481464968550284 1102 66-67 0.2163858429092329 1102 68-69 0.23482523747583883 1102 70-71 0.1859135300143393 1102 72-73 0.16507927850220483 1102 74-75 0.051131773730794805 1102 76-77 -0.04135627412279774 1102 78-79 0.10027412398561353 1102 80-81 0.13280686065293423 1102 82-83 0.13451059581869984 1102 84-85 0.18247650905276558 1102 86-87 0.12259277167779103 1102 88-89 0.22816779274274523 1102 90-91 0.18131269905205372 1102 92-93 0.15582453757736303 1102 94-95 0.1454116895604436 1102 96-97 0.1904372729655357 1102 98-99 0.27037848751917437 1102 100-101 0.25316672069670076 1103 1 0.11212934777776695 1103 2 0.05561104644487358 1103 3 0.09418410481608674 1103 4 0.09630640706843252 1103 5 0.04923641652141697 1103 6 0.09522292365658558 1103 7 0.07992501348503822 1103 8 0.09930840836720733 1103 9 0.10697010758578074 1103 10-11 0.05944263601858779 1103 12-13 0.1151356779889312 1103 14-15 0.18454815987906414 1103 16-17 0.05207731876205912 1103 18-19 0.1060146021246311 1103 20-21 0.062955190333561 1103 22-23 0.05751897660078953 1103 24-25 0.11341311209129401 1103 26-27 0.004899152047109112 1103 28-29 -0.0679922226372156 1103 30-31 -0.08658009453111504 1103 32-33 0.030809923900989133 1103 34-35 0.09010698660895855 1103 36-37 0.15887409573617362 1103 38-39 0.21157597364156544 1103 40-41 0.04763573777557184 1103 42-43 0.11154498951783154 1103 44-45 0.10555730522873574 1103 46-47 0.05145000388581167 1103 48-49 -0.05614084516356144 1103 50-51 -0.07555230320657103 1103 52-53 0.16402647291138805 1103 54-55 -0.03251550014174143 1103 56-57 -0.0682322936293076 1103 58-59 -0.09093095395507333 1103 60-61 -0.05914997478542716 1103 62-63 -0.004860516856709296 1103 64-65 0.028488472511668306 1103 66-67 0.08165118679633565 1103 68-69 0.06287342589821066 1103 70-71 0.037617036583334595 1103 72-73 0.059613196122491274 1103 74-75 0.03364319935366922 1103 76-77 -0.23738471574748132 1103 78-79 0.09765008586462898 1103 80-81 0.06707013038914056 1103 82-83 0.03542985644491026 1103 84-85 -0.028962966919106492 1103 86-87 0.07021092964878406 1103 88-89 0.06466763768979789 1103 90-91 0.10122636929934359 1103 92-93 0.04358653892963105 1103 94-95 0.007086244439872047 1103 96-97 0.24135839761624567 1103 98-99 0.03948622135681035 1103 100-101 0.17158302853865237 1104 1 0.06360266352358934 1104 2 0.09761010791836355 1104 3 0.05522057634196642 1104 4 0.06474724813190136 1104 5 -0.039179616245519355 1104 6 0.013993497739491545 1104 7 0.042555709436179256 1104 8 0.08857625691128845 1104 9 0.11031870183342818 1104 10-11 0.13846814580016087 1104 12-13 0.047651042964893975 1104 14-15 0.11456801703200625 1104 16-17 0.057300884803524355 1104 18-19 0.049967108827303264 1104 20-21 -0.032092745937774225 1104 22-23 -0.0880694010934775 1104 24-25 -0.04978534420347103 1104 26-27 -0.07979004977158155 1104 28-29 -0.1240160556022758 1104 30-31 0.010096461278124025 1104 32-33 -0.03569839380118367 1104 34-35 0.06283122412188646 1104 36-37 0.03793419779983509 1104 38-39 0.09423321298028497 1104 40-41 0.1322172631747236 1104 42-43 0.23393857388729344 1104 44-45 0.33472471128078496 1104 46-47 0.35938627083745445 1104 48-49 0.28943386211650335 1104 50-51 0.40353974030565354 1104 52-53 0.3943044905786408 1104 54-55 0.3526935498477144 1104 56-57 0.34503330981213054 1104 58-59 0.21866646549135282 1104 60-61 0.1313008027412863 1104 62-63 0.29953973534490785 1104 64-65 0.22044036667461597 1104 66-67 0.170848996375355 1104 68-69 0.21219148254367326 1104 70-71 0.1048556479671987 1104 72-73 0.2767615915357098 1104 74-75 0.2007804437399301 1104 76-77 0.1744277489537147 1104 78-79 0.0949585039917693 1104 80-81 0.23074672256518625 1104 82-83 0.16978209569819214 1104 84-85 0.15608843515274984 1104 86-87 0.15391428428956289 1104 88-89 0.15480101624065767 1104 90-91 0.31941779102680457 1104 92-93 0.34526530522732557 1104 94-95 -0.04509705653577001 1104 96-97 0.056101829792808644 1104 98-99 0.13176644644543956 1104 100-101 0.3614498559203163 1105 1 -0.0025260575315684264 1105 2 0.023631351013399637 1105 3 0.027161492048470848 1105 4 0.049077904838433994 1105 5 -0.1151523846843503 1105 6 -0.008984918532746633 1105 7 -0.0016183991422806798 1105 8 -0.004414569825762271 1105 9 0.05207241246712613 1105 10-11 0.06278265222811541 1105 12-13 -0.012149889368302524 1105 14-15 0.09638656698471237 1105 16-17 0.07344806672283966 1105 18-19 0.06126611051140429 1105 20-21 0.026747983254445273 1105 22-23 0.0028904923813826144 1105 24-25 0.012307593741816447 1105 26-27 0.22309008324192092 1105 28-29 0.06832399589757898 1105 30-31 0.015198925029324073 1105 32-33 0.06867671190214253 1105 34-35 -0.012220644951277393 1105 36-37 0.10687397250583075 1105 38-39 0.2514293769318172 1105 40-41 0.22706977873544076 1105 42-43 -0.014981763315283558 1105 44-45 0.09093744713320717 1105 46-47 0.18481390782302043 1105 48-49 0.3656491229387413 1105 50-51 0.41711335865884536 1105 52-53 0.39823331977873266 1105 54-55 0.37712656311333603 1105 56-57 0.29882207464931554 1105 58-59 0.3090626475464404 1105 60-61 0.22162087839510036 1105 62-63 0.23396888969440255 1105 64-65 0.3282813507458755 1105 66-67 0.06939237464203529 1105 68-69 0.20728256011027213 1105 70-71 0.11884005032351297 1105 72-73 0.21950408987423486 1105 74-75 0.05757713841152423 1105 76-77 0.15915180072035895 1105 78-79 0.23235009107821725 1105 80-81 0.10745816377507822 1105 82-83 0.20886755639798693 1105 84-85 0.20450544582670105 1105 86-87 0.16121037511225111 1105 88-89 0.22222497534567154 1105 90-91 0.24927150952125388 1105 92-93 0.3826230672465414 1105 94-95 0.3370885668578403 1105 96-97 0.3593343866582188 1105 98-99 0.3029913188272708 1105 100-101 0.34277675137465735 1106 1 -0.27106505577343043 1106 2 -0.1233091143822378 1106 3 -0.16426417206311328 1106 4 -0.020777799771494188 1106 5 -0.19927493517607076 1106 6 -0.09302116586128761 1106 7 -0.11088636754832493 1106 8 -0.16192892210010967 1106 9 -0.23074188703400722 1106 10-11 -0.263582380995679 1106 12-13 -0.4438279610757334 1106 14-15 -0.26026941869362474 1106 16-17 -0.22959470290894757 1106 18-19 -0.10803795360951085 1106 20-21 -0.3505171103232527 1106 22-23 -0.2972552899888683 1106 24-25 -0.21461884738980075 1106 26-27 -0.1379157567998348 1106 28-29 -0.08663887333272413 1106 30-31 -0.11315239298907898 1106 32-33 -0.14614789732820554 1106 34-35 -0.08048382294863643 1106 36-37 -0.19167636760443685 1106 38-39 -0.1524272956730499 1106 40-41 -0.22741332071437625 1106 42-43 -0.30098636420688507 1106 44-45 -0.15525661252676315 1106 46-47 -0.08519632609937133 1106 48-49 -0.22653402636864683 1106 50-51 0.008516572285415691 1106 52-53 0.08474626379538819 1106 54-55 -0.007995908655907158 1106 56-57 -0.04534735945268409 1106 58-59 -0.13974864300656975 1106 60-61 0.01910146747250252 1106 62-63 0.07414067963950544 1106 64-65 0.05406646170083462 1106 66-67 0.026551892627196594 1106 68-69 0.10425028680914039 1106 70-71 0.0058681892366223565 1106 72-73 0.01604030075718299 1106 74-75 -0.14373883904468698 1106 76-77 -0.01828298353631297 1106 78-79 -0.06103824108399891 1106 80-81 -0.055026784195192135 1106 82-83 -0.2698725632018686 1106 84-85 -0.19502950841221534 1106 86-87 -0.056304064631522266 1106 88-89 7.94529572342384E-4 1106 90-91 0.05730353499234653 1106 92-93 0.013006299941444865 1106 94-95 -0.13707063332176617 1106 96-97 0.15948381358772323 1106 98-99 0.09913391731055299 1106 100-101 0.26176577979347115 1107 1 -0.1383889100668867 1107 2 -0.08036969964417295 1107 3 -0.06821683630645481 1107 4 -0.012665942400836627 1107 5 -0.09002578655476157 1107 6 -0.14971500337945542 1107 7 -0.08637592325548837 1107 8 -0.004001026116576156 1107 9 -0.0015404446495494994 1107 10-11 -0.005915998409911083 1107 12-13 -0.0665284437367788 1107 14-15 -0.12065851966399066 1107 16-17 -0.10840396159604637 1107 18-19 -0.07773771950196817 1107 20-21 -0.1769054952162321 1107 22-23 -0.09927129666856871 1107 24-25 -0.10975377199086722 1107 26-27 -0.08559372380359775 1107 28-29 -0.26311082362754945 1107 30-31 -0.22526757915191808 1107 32-33 -0.1929089258531107 1107 34-35 -0.019745979848380557 1107 36-37 -0.07468912497930802 1107 38-39 -0.14137025900311073 1107 40-41 -0.2012839263596362 1107 42-43 -0.23549310369779874 1107 44-45 -0.2890518019520485 1107 46-47 -0.36180627335432547 1107 48-49 -0.39207473737267406 1107 50-51 -0.338780097332652 1107 52-53 -0.34823812004974286 1107 54-55 -0.28663796741987113 1107 56-57 -0.16851560791771902 1107 58-59 -0.32375513055711735 1107 60-61 -0.3609336244667247 1107 62-63 -0.4414139589314914 1107 64-65 -0.41857675550493667 1107 66-67 -0.2579366338915037 1107 68-69 -0.2827969722249364 1107 70-71 -0.44764000245370283 1107 72-73 -0.34754954863847587 1107 74-75 -0.4119675359151671 1107 76-77 -0.27757540092648725 1107 78-79 -0.27523938279889393 1107 80-81 -0.18003015376784504 1107 82-83 -0.1365306244983664 1107 84-85 -0.16549736407147364 1107 86-87 -0.03617855755403454 1107 88-89 -0.09118892876349705 1107 90-91 0.05862388907741334 1107 92-93 -0.004565935056351833 1107 94-95 0.03652750349463574 1107 96-97 0.19744973908664676 1107 98-99 0.17834393050545216 1107 100-101 0.2481326616289543 1108 1 -0.07291086185447426 1108 2 0.040127175477131516 1108 3 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-0.8071526791208967 2316 68-69 -0.7083483078283948 2316 70-71 -0.4820572790590347 2316 72-73 -0.5915947484239865 2316 74-75 -0.5593410141992976 2316 76-77 -0.4259532999140383 2316 78-79 -0.5170227148229785 2316 80-81 -0.7171925813180628 2316 82-83 -0.7342429133252502 2316 84-85 -0.6911867968484309 2316 86-87 -0.5469770051277756 2316 88-89 -0.5019841886036325 2316 90-91 -0.5276097125947814 2316 92-93 -0.5782390124829853 2316 94-95 -0.5995480207452353 2316 96-97 -0.6654270538711948 2316 98-99 -0.7117102837352896 2316 100-101 -0.9669534014858954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 10.0 5 27.0 6 44.0 7 96.0 8 254.0 9 492.0 10 842.0 11 1397.0 12 2101.0 13 3008.0 14 3855.0 15 5168.0 16 6907.0 17 9528.0 18 8416.0 19 5259.0 20 4882.0 21 4987.0 22 5249.0 23 5816.0 24 6890.0 25 8327.0 26 10348.0 27 13168.0 28 16574.0 29 21124.0 30 27394.0 31 34855.0 32 45355.0 33 60638.0 34 85536.0 35 125945.0 36 202779.0 37 342028.0 38 323635.0 39 34188.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87623032186061 19.65830001065578 15.270712871620296 23.194756795863313 2 15.346345947945805 20.591916071999147 39.978264037983976 24.08347394207107 3 18.933830742582305 24.16146274195447 30.422568963680902 26.48213755178232 4 12.928903363958943 17.122010078983806 37.595296287368754 32.353790269688496 5 13.781535807213652 37.21472274838839 34.88064729300646 14.123094151391497 6 32.17826291685119 36.70147316848944 17.43032159694155 13.68994231771782 7 28.576553749908907 32.38364043029077 21.181701991692407 17.85810382810792 8 26.088059375192696 35.50760688599761 19.981739549640956 18.422594189168738 9 25.883732923746155 16.904650458823596 20.443646260181286 36.76797035724896 10-11 24.538023218660136 26.84202117819845 28.114541933169274 20.50541366997214 12-13 25.39045076982964 23.827806787002295 28.658201804929305 22.123540638238754 14-15 22.30339535066231 25.570025954224146 26.40562023868917 25.72095845642437 16-17 22.124644741046353 28.914540251470083 27.093365136133553 21.86744987135001 18-19 21.883881473841168 27.60099304337102 29.265279638546787 21.249845844241023 20-21 22.316183271577604 26.673851259312407 29.5680555633412 21.44190990576879 22-23 22.146279902726253 26.529379552261865 29.355903010742367 21.968437534269516 24-25 22.218344956372 26.777869454093818 29.302319877936196 21.70146571159799 26-27 22.061134361407117 27.03695074460314 29.13504811322242 21.766866780767323 28-29 21.81862008993107 27.13666873847745 29.34805229932457 21.69665887226691 30-31 22.025376947450884 26.91700499326617 29.264386646691733 21.793231412591215 32-33 22.012496426389223 26.88763726869629 29.141884960564152 21.95798134435033 34-35 22.373185867000018 26.811014849403836 29.017754539186395 21.79804474440975 36-37 22.266709456628217 26.713128343257708 29.091474754156255 21.92868744595782 38-39 22.09684985878967 26.988462480375773 29.160834726283902 21.75385293455065 40-41 22.326238431310998 26.72636932356453 29.154707916879214 21.792684328245258 42-43 22.132965279843642 26.879082305015572 28.905198545305723 22.082753869835066 44-45 22.275296960048433 27.021472495809768 28.60374122012882 22.099489324012982 46-47 22.1167267406988 27.145182492390312 28.44762347876294 22.290467288147944 48-49 22.36740502603831 27.258592081439087 28.31017960547337 22.06382328704923 50-51 22.399742700024106 27.22881199163635 28.208892264744296 22.16255304359525 52-53 22.608604560070688 27.277144921229855 27.948143367759144 22.166107150940313 54-55 22.270987605876975 27.189537308496504 28.20629964516147 22.333175440465045 56-57 22.284826588785307 27.425570796733016 28.148701447943004 22.140901166538672 58-59 22.400408372619694 27.12510720832329 28.225113654836846 22.24937076422017 60-61 22.37332600859909 27.141573846214218 28.548140040697124 21.936960104489575 62-63 22.283390137394825 27.186839582714374 28.444890717581046 22.08487956230976 64-65 22.14629661810293 27.060887320548684 28.329484110745497 22.46333195060289 66-67 22.022726763121458 27.40412913207505 28.243051779518026 22.33009232528547 68-69 22.122157227662832 27.107169083642113 28.399975335078565 22.370698353616493 70-71 22.16384935338666 27.147214545576855 28.256330196030067 22.43260590500642 72-73 22.15151689266835 27.089896631556527 28.278472568683398 22.480113907091727 74-75 22.03379794944812 27.20260551260994 28.043420071640384 22.72017646630155 76-77 22.13385905118532 27.108500428833292 28.169442404605615 22.588198115375775 78-79 22.088207925287712 27.0911228705484 28.15532313849914 22.66534606566475 80-81 21.967826291685117 27.404724733871106 27.979270254665312 22.648178719778464 82-83 21.97549748829635 27.184138113219706 28.166466846843186 22.673897551640753 84-85 22.041381040369917 27.01367853036205 27.998454237079113 22.94648619218892 86-87 22.047987286354132 26.915315234512953 27.98102202465371 23.055675454479204 88-89 21.944948175636664 26.737860934688406 28.140888553794753 23.176302335880173 90-91 21.69280264382379 26.76812022783079 28.503199665878082 23.035877462467344 92-93 21.58880691978676 26.527507609859462 28.530771580888743 23.35291388946504 94-95 21.337644556059445 26.692349950389875 28.560192218217285 23.409813275333395 96-97 21.45711526926807 26.833157222057167 28.383158343189958 23.326569165484806 98-99 21.2692210804832 26.794542682894786 28.173236639183415 23.762999597438604 100-101 20.77131424406789 26.963972865772472 28.351203240409816 23.91350964974982 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 59.0 1 49.5 2 52.5 3 73.0 4 74.0 5 81.5 6 148.5 7 323.5 8 540.0 9 698.5 10 736.0 11 734.0 12 800.0 13 912.0 14 1042.5 15 1142.5 16 1236.0 17 1362.5 18 1468.0 19 1542.5 20 1711.0 21 2012.0 22 2387.5 23 2848.5 24 3560.0 25 4568.5 26 5907.0 27 7904.0 28 10067.5 29 12536.5 30 15693.0 31 18942.0 32 22576.5 33 26023.5 34 29682.5 35 33037.5 36 36542.5 37 40257.0 38 43530.0 39 45556.5 40 47172.0 41 50360.0 42 53488.5 43 55819.5 44 57090.5 45 58388.0 46 58283.5 47 57639.5 48 59106.0 49 58940.0 50 57736.0 51 56135.5 52 51841.0 53 48169.5 54 45057.5 55 40502.0 56 35260.0 57 30306.0 58 26786.5 59 23148.5 60 18966.5 61 15178.5 62 11562.0 63 8484.5 64 6163.5 65 4524.0 66 3360.5 67 2675.5 68 1939.0 69 1231.0 70 887.5 71 675.0 72 519.5 73 402.0 74 318.0 75 238.0 76 163.5 77 94.5 78 52.0 79 32.0 80 16.0 81 8.5 82 6.5 83 4.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04708757728809189 2 0.0 3 0.0 4 0.0 5 0.009739841135483292 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006411478157530369 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001506522190020797 24-25 3.8538939744718067E-4 26-27 0.001786805388164201 28-29 0.0024524779837547857 30-31 4.204247972151062E-4 32-33 0.0 34-35 0.0 36-37 1.4014159907170203E-4 38-39 1.0510619930377655E-4 40-41 0.0 42-43 0.01131643412503994 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.007079953585102E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.306371958226592E-4 84-85 7.007079953585102E-5 86-87 0.0 88-89 0.0 90-91 0.0072873631517285055 92-93 0.0022422655851472325 94-95 0.0 96-97 0.0 98-99 0.0011561681923415417 100-101 0.01037047833130595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1427128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.42950833314662 #Duplication Level Percentage of deduplicated Percentage of total 1 77.13520619185081 45.8410737915758 2 13.162771369663837 15.645140616014814 3 4.294256309166193 7.65616623330779 4 1.889215435193245 4.491005777957047 5 0.9975674352738587 2.964247110374174 6 0.6064881746573636 2.1625976417852804 7 0.3742884578460345 1.557064531719305 8 0.26451187316366553 1.2575848456317027 9 0.19190494087564572 1.0264334654647076 >10 0.984735864711447 10.49701427927804 >50 0.06574310551582715 2.666476218302204 >100 0.032365879208439086 3.3504155014075985 >500 5.906017959942703E-4 0.26352460106944925 >1k 3.543610775965622E-4 0.6212553861120473 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3823 0.2678806666255585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.2107729650038399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2019 0.14147294426288323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.0510619930377652E-4 0.0 22-23 0.0 0.0 0.0 1.4014159907170203E-4 0.0 24-25 0.0 0.0 0.0 2.1021239860755306E-4 0.0 26-27 0.0 0.0 0.0 4.5546019698303165E-4 0.0 28-29 0.0 0.0 0.0 0.0011211327925736165 0.0 30-31 0.0 0.0 0.0 0.002872902780969892 0.0 32-33 0.0 0.0 0.0 0.005570628563100156 0.0 34-35 0.0 0.0 0.0 0.009074168539892707 0.0 36-37 0.0 0.0 0.0 0.013243381112275843 0.0 38-39 0.0 0.0 0.0 0.01919939907282318 0.0 40-41 0.0 0.0 0.0 0.025961231228032806 0.0 42-43 0.0 0.0 0.0 0.0361214971607312 0.0 44-45 0.0 0.0 0.0 0.0460014798952862 0.0 46-47 0.0 0.0 0.0 0.06628697636091507 0.0 48-49 0.0 0.0 0.0 0.09130225179521388 0.0 50-51 0.0 0.0 0.0 0.10500109310447275 0.0 52-53 0.0 0.0 0.0 0.12872005874735834 0.0 54-55 0.0 0.0 0.0 0.1661729010992707 0.0 56-57 0.0 0.0 0.0 0.22944683308014419 0.0 58-59 0.0 0.0 0.0 0.3143025713180597 0.0 60-61 0.0 0.0 0.0 0.4180423900308872 0.0 62-63 0.0 0.0 0.0 0.537618209438817 0.0 64-65 0.0 0.0 0.0 0.6828749768766362 0.0 66-67 0.0 0.0 0.0 0.8732573392155434 0.0 68-69 0.0 0.0 0.0 1.1046661546826915 0.0 70-71 0.0 0.0 0.0 1.243161089965301 0.0 72-73 0.0 0.0 0.0 1.4105251946566812 0.0 74-75 0.0 0.0 0.0 1.6413734437275425 0.0 76-77 0.0 0.0 0.0 1.9020017826011402 0.0 78-79 0.0 0.0 0.0 2.157059492911638 0.0 80-81 0.0 0.0 0.0 2.5459524303356114 0.0 82-83 0.0 0.0 0.0 2.9644152451637136 0.0 84-85 0.0 0.0 0.0 3.5227043404656064 0.0 86-87 0.0 0.0 0.0 4.307252047468762 0.0 88-89 0.0 0.0 0.0 5.13703045557231 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2395 0.0 43.652065 1 GTATCAA 4205 0.0 41.362114 1 ATCAACG 5160 0.0 33.141563 3 TCAACGC 5245 0.0 32.604473 4 TATCAAC 5340 0.0 32.38026 2 CAACGCA 5365 0.0 31.875204 5 AACGCAG 5455 0.0 31.346012 6 GTACCGT 120 5.3847107E-6 27.707117 6 ACGCAGA 6270 0.0 27.04711 7 CGCAGAG 6440 0.0 26.111849 8 GTACATG 3870 0.0 23.453629 1 GCAGAGT 7035 0.0 23.430716 9 TACATGG 4070 0.0 21.242086 2 GAGTACT 4180 0.0 21.194479 12-13 GTACTTT 4380 0.0 20.170347 14-15 AGAGTAC 6335 0.0 20.133434 10-11 AGTACTT 4355 0.0 20.015575 12-13 GTATAGA 475 0.0 20.008936 1 ACATGGG 3975 0.0 19.71819 3 CAGCGTA 145 6.144501E-4 19.656376 9 >>END_MODULE