FastQCFastQC Report
Thu 26 May 2016
SRR937241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548196
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.19041516707186945No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22640.14623471446767722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22040.14235923616906385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.039.3346981
GTATCAA40250.036.9731451
TCAACGC48800.030.0938474
ATCAACG49150.029.9752753
TATCAAC50950.029.1970672
CAACGCA51050.028.7656175
AACGCAG52600.027.9956156
GTACATG45300.024.4548871
ACGCAGA58750.024.3381047
CGCAGAG59600.023.830828
TACATGG47550.022.6889512
ACATGGG46000.021.9029733
GCAGAGT66100.021.0541659
GAGTACT33800.019.95559112-13
CGTAACA1707.921632E-519.5698812
CGCGGAA1101.349199E-619.42738582-83
TGCACCG2952.9995135E-919.3317055
AGAGTAC58000.019.12281410-11
CATGGGG26150.019.083444
GTACTTT35500.018.93276214-15
Produced by FastQC (version 0.11.5)