##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937241_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548196 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80656841898571 33.0 31.0 34.0 30.0 34.0 2 31.916063599182532 33.0 31.0 34.0 30.0 34.0 3 31.97457363279585 33.0 31.0 34.0 30.0 34.0 4 35.58990915878868 37.0 35.0 37.0 33.0 37.0 5 35.56673379856297 37.0 35.0 37.0 33.0 37.0 6 35.62222741823387 37.0 35.0 37.0 33.0 37.0 7 35.570472989208085 37.0 35.0 37.0 33.0 37.0 8 35.55516032853721 37.0 35.0 37.0 33.0 37.0 9 37.212761174941676 39.0 37.0 39.0 34.0 39.0 10-11 37.115154670338896 39.0 37.0 39.0 33.0 39.0 12-13 37.009830473660955 39.0 37.0 39.0 33.0 39.0 14-15 38.24043854912427 40.0 38.0 41.0 33.0 41.0 16-17 38.18301429534762 40.0 38.0 41.0 33.0 41.0 18-19 38.10948129306625 40.0 38.0 41.0 33.0 41.0 20-21 38.033733454937234 40.0 38.0 41.0 33.0 41.0 22-23 37.90482309733393 40.0 38.0 41.0 33.0 41.0 24-25 37.772832703352805 40.0 37.5 41.0 33.0 41.0 26-27 37.62538141165589 40.0 37.0 41.0 32.0 41.0 28-29 37.45255187327703 40.0 37.0 41.0 32.0 41.0 30-31 37.48078667042157 40.0 37.0 41.0 32.0 41.0 32-33 37.38660608863477 40.0 37.0 41.0 32.0 41.0 34-35 37.2230247333025 39.5 37.0 41.0 31.5 41.0 36-37 37.28840340628706 40.0 37.0 41.0 31.5 41.0 38-39 37.30735223447161 40.0 37.0 41.0 32.0 41.0 40-41 37.18210032838219 40.0 37.0 41.0 31.5 41.0 42-43 37.00926336200326 40.0 37.0 41.0 31.0 41.0 44-45 36.85658146642932 40.0 36.0 41.0 31.0 41.0 46-47 36.640397598236916 39.5 36.0 41.0 30.0 41.0 48-49 36.427025066593636 39.0 36.0 41.0 30.0 41.0 50-51 35.52389361553705 38.5 35.0 40.0 29.0 40.5 52-53 35.65390299419453 38.5 35.0 40.0 29.0 40.5 54-55 36.03559917478149 39.0 35.0 40.0 29.0 41.0 56-57 35.851520737684375 39.0 35.0 40.5 28.5 41.0 58-59 35.63347114964772 39.0 35.0 40.0 28.0 41.0 60-61 35.37551479270066 38.0 35.0 40.0 28.0 41.0 62-63 35.06959648519955 38.0 34.0 40.0 27.5 41.0 64-65 34.73096494242331 37.5 34.0 40.0 26.5 41.0 66-67 34.36823955106459 37.0 34.0 40.0 26.0 41.0 68-69 33.90461091489708 36.5 33.5 39.0 26.0 41.0 70-71 33.48464503202437 36.0 33.0 39.0 25.0 40.5 72-73 33.07585215308656 35.5 33.0 38.5 24.5 40.0 74-75 32.63656959454746 35.0 32.0 37.5 23.5 39.5 76-77 32.20295233936788 35.0 32.0 37.0 22.5 39.0 78-79 31.74626823735496 35.0 31.5 36.5 21.5 39.0 80-81 31.301889424853183 35.0 31.0 36.0 20.0 38.0 82-83 30.87815205568287 34.0 31.0 35.5 18.5 37.0 84-85 30.438777454534183 34.0 30.5 35.0 17.5 37.0 86-87 30.030480313862068 34.0 30.0 35.0 13.0 36.0 88-89 29.680150639841468 34.0 30.0 35.0 7.5 36.0 90-91 29.290054037085746 34.0 29.0 35.0 4.5 35.5 92-93 28.885758004800426 34.0 29.0 35.0 2.0 35.0 94-95 28.401269606690626 34.0 29.0 35.0 2.0 35.0 96-97 27.826389875700492 34.0 28.0 35.0 2.0 35.0 98-99 27.164509532384788 33.0 27.0 35.0 2.0 35.0 100-101 25.339995711137348 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15920982686209229 1101 2 0.0978228324085606 1101 3 0.2053344352909825 1101 4 0.2228593018426679 1101 5 0.1504400937566288 1101 6 0.016244342971560854 1101 7 0.1393009711520321 1101 8 0.12300049477781272 1101 9 0.25801611126877333 1101 10-11 0.188100269836319 1101 12-13 0.23835117285376128 1101 14-15 0.35630427592081304 1101 16-17 0.337864953004285 1101 18-19 0.4198167149119101 1101 20-21 0.3769035680474033 1101 22-23 0.3061965407434002 1101 24-25 0.3845866986562285 1101 26-27 0.39467217377307406 1101 28-29 0.43931263612783056 1101 30-31 0.31314068116101623 1101 32-33 0.20340928105208889 1101 34-35 0.19520988397609784 1101 36-37 0.26575958087196483 1101 38-39 0.23943317729707303 1101 40-41 0.3296704659801435 1101 42-43 0.4060872035528149 1101 44-45 0.26450603434758335 1101 46-47 0.316845689533622 1101 48-49 0.39714190674978767 1101 50-51 0.6012772358638827 1101 52-53 0.6440578661984233 1101 54-55 0.7018390362427667 1101 56-57 0.7481760933788166 1101 58-59 0.6964050866470757 1101 60-61 0.6995577848739103 1101 62-63 0.6333175303659857 1101 64-65 0.5534922319231868 1101 66-67 0.665515927023371 1101 68-69 0.702780118207528 1101 70-71 0.626577200568093 1101 72-73 0.5902784905921976 1101 74-75 0.539935822628415 1101 76-77 0.5663619061869269 1101 78-79 0.7008948326178022 1101 80-81 0.7721725136987558 1101 82-83 0.7329883687912435 1101 84-85 0.71466609331247 1101 86-87 0.8678208681702557 1101 88-89 0.6852270926531503 1101 90-91 0.6689549946756053 1101 92-93 0.7461475831437809 1101 94-95 0.6626971154325396 1101 96-97 0.5966057844867017 1101 98-99 0.7700521139630112 1101 100-101 1.1187810285053708 1102 1 0.05735682693843813 1102 2 0.016175795964912254 1102 3 -0.018742489410289664 1102 4 -0.04351361084998473 1102 5 -0.002793140148675377 1102 6 0.04817122718149136 1102 7 0.06381580152246613 1102 8 0.08562609508651065 1102 9 0.11667471540316399 1102 10-11 0.10518137037053066 1102 12-13 0.22074813381043867 1102 14-15 0.20308511968199383 1102 16-17 0.31138016978862026 1102 18-19 0.1995801956763117 1102 20-21 0.362631160508343 1102 22-23 0.23908027311540536 1102 24-25 0.3243966429393552 1102 26-27 0.3502035325873436 1102 28-29 0.21244138487054443 1102 30-31 0.1981164901350212 1102 32-33 0.17781454927585116 1102 34-35 0.2661909222617638 1102 36-37 0.26328263350205106 1102 38-39 0.44414997164636816 1102 40-41 0.37120788432741847 1102 42-43 0.20677677458284904 1102 44-45 0.35513038066106617 1102 46-47 0.3989162291107249 1102 48-49 0.4846722346457142 1102 50-51 0.43302899751238755 1102 52-53 0.49162399107346033 1102 54-55 0.5956798947205257 1102 56-57 0.5381978614218212 1102 58-59 0.5567812563342116 1102 60-61 0.46369442414868445 1102 62-63 0.6444625301503137 1102 64-65 0.5004057125702772 1102 66-67 0.49379429519080986 1102 68-69 0.699163240818045 1102 70-71 0.4766222581249977 1102 72-73 0.40070024123665604 1102 74-75 0.3969414740895729 1102 76-77 0.3205256016537916 1102 78-79 0.43255980003793937 1102 80-81 0.3146710035080602 1102 82-83 0.32102819992422127 1102 84-85 0.26289049348205396 1102 86-87 0.27283307693627634 1102 88-89 0.43718618945172594 1102 90-91 0.4157613948159451 1102 92-93 0.23972888915777446 1102 94-95 -0.044926446321756686 1102 96-97 0.162423748347166 1102 98-99 0.4728838546249605 1102 100-101 0.8537548792398724 1103 1 0.07619922283488378 1103 2 0.09695892057473188 1103 3 0.1994542902315608 1103 4 0.05663377206885656 1103 5 0.04804412906558042 1103 6 0.0032228387856676477 1103 7 0.07381559005602156 1103 8 0.035206242248769115 1103 9 0.16345421933966264 1103 10-11 0.07170457697417021 1103 12-13 0.1962450569736447 1103 14-15 0.1579206677432623 1103 16-17 0.19328204384104453 1103 18-19 0.07190273165549144 1103 20-21 0.16389646539239067 1103 22-23 0.15510611983165745 1103 24-25 0.06710459030811222 1103 26-27 -0.08841106797552811 1103 28-29 -0.09347415910850287 1103 30-31 -0.03355070213303435 1103 32-33 -0.03854228839974638 1103 34-35 0.0737953476108899 1103 36-37 0.13351266302459663 1103 38-39 0.12396044254536775 1103 40-41 0.1761423159377955 1103 42-43 0.1562410749093317 1103 44-45 0.10101841561370861 1103 46-47 0.16862522369810762 1103 48-49 0.15426188860385537 1103 50-51 0.010678465214560617 1103 52-53 0.08716866887674968 1103 54-55 0.1773528333594001 1103 56-57 0.20718483600815318 1103 58-59 0.14494391632466375 1103 60-61 0.24119090047409486 1103 62-63 0.23315560315312922 1103 64-65 0.180095279827178 1103 66-67 0.2878855549148085 1103 68-69 0.20572964174188257 1103 70-71 0.3610715673192786 1103 72-73 0.41061921281097113 1103 74-75 0.35104084309208616 1103 76-77 0.3495797122397093 1103 78-79 0.3873282304880057 1103 80-81 0.05439157358482305 1103 82-83 0.23399881620180452 1103 84-85 0.27370543919493784 1103 86-87 0.516806145305214 1103 88-89 0.5445826421480611 1103 90-91 0.586160413611637 1103 92-93 0.5889419121809567 1103 94-95 0.5803122579221913 1103 96-97 0.8396272976735553 1103 98-99 0.9931988844647144 1103 100-101 1.2971465987654902 1104 1 -0.049946824449126126 1104 2 -0.07270900369704592 1104 3 -0.1335342942100688 1104 4 -0.21756298729156498 1104 5 -0.11522117705686696 1104 6 -0.028143483617668608 1104 7 0.0068740953996311305 1104 8 0.05709731131022977 1104 9 0.031675654951733634 1104 10-11 0.01817235751965285 1104 12-13 0.10650934827188507 1104 14-15 0.13497592953391546 1104 16-17 0.029303932967856383 1104 18-19 0.02367187614412103 1104 20-21 0.013775803280296373 1104 22-23 0.01707546145838279 1104 24-25 -0.01200993915821158 1104 26-27 0.06701921263064747 1104 28-29 -0.16452107894710366 1104 30-31 -0.26016628769630756 1104 32-33 -0.1716216729015798 1104 34-35 0.036124004983896896 1104 36-37 -0.06471455077312527 1104 38-39 -0.026093697597488585 1104 40-41 -0.1940767209988934 1104 42-43 -0.17069061139830666 1104 44-45 -0.05412295570997827 1104 46-47 0.01497501272149293 1104 48-49 -0.03165341970362334 1104 50-51 -0.338069028072546 1104 52-53 -0.23022075655794794 1104 54-55 -0.14465137160595276 1104 56-57 -0.19373627476306154 1104 58-59 -0.22474682935261825 1104 60-61 -0.1642216571256725 1104 62-63 -0.05751723985987667 1104 64-65 -0.1725585582962168 1104 66-67 -0.20410316062850598 1104 68-69 -0.15278201416959547 1104 70-71 -0.2114694262373149 1104 72-73 -0.28054987438137147 1104 74-75 -0.24757336347562386 1104 76-77 -0.11872815337620324 1104 78-79 -0.13559222963037953 1104 80-81 -0.1487223343623647 1104 82-83 -0.023303790779607425 1104 84-85 0.08259122048818313 1104 86-87 -0.021797476014832284 1104 88-89 -0.18447434824267006 1104 90-91 -0.15508757737633871 1104 92-93 -0.06149096618095129 1104 94-95 0.08133096661908823 1104 96-97 0.10509074215198666 1104 98-99 0.2308571169225786 1104 100-101 0.5581661814736627 1105 1 0.09636138093006963 1105 2 0.11146047372396595 1105 3 0.1308799749368923 1105 4 0.08193177250118566 1105 5 0.12846799967349654 1105 6 0.10632376303187385 1105 7 0.20609732810226689 1105 8 0.18894186173496763 1105 9 0.18645014329327125 1105 10-11 0.19183460918365114 1105 12-13 0.32170285084487915 1105 14-15 0.24026874753488414 1105 16-17 0.2310576666234354 1105 18-19 0.32218726668404685 1105 20-21 0.3134022164036452 1105 22-23 0.3242904686733965 1105 24-25 0.3420989473783749 1105 26-27 0.2646383234837657 1105 28-29 0.288910823652202 1105 30-31 0.18458155380876207 1105 32-33 0.16011272377583907 1105 34-35 0.2790748607305531 1105 36-37 0.25583740676393774 1105 38-39 0.3267868039245627 1105 40-41 0.4166868556717773 1105 42-43 0.3913334099596284 1105 44-45 0.2528224214369743 1105 46-47 0.267201294570917 1105 48-49 0.36804950664259906 1105 50-51 0.2567910539041023 1105 52-53 0.159787501811401 1105 54-55 0.286832238755828 1105 56-57 0.36899002415955806 1105 58-59 0.41383101115335563 1105 60-61 0.5136581804386466 1105 62-63 0.48832733332912426 1105 64-65 0.5105121639506578 1105 66-67 0.6312866710803462 1105 68-69 0.5379581153084843 1105 70-71 0.50029046641653 1105 72-73 0.470398320475681 1105 74-75 0.3189895993187193 1105 76-77 0.3453478135043184 1105 78-79 0.3896171699874351 1105 80-81 0.3679637451633262 1105 82-83 0.49266749083584926 1105 84-85 0.4250439182432473 1105 86-87 0.34710957369852835 1105 88-89 0.5551352193248285 1105 90-91 0.5834881131713097 1105 92-93 0.35647446501848634 1105 94-95 0.4283257675139751 1105 96-97 0.4330247713130113 1105 98-99 0.404982874308633 1105 100-101 0.7009996747891911 1106 1 -0.09190529428303051 1106 2 0.004139791202966592 1106 3 -0.15764960762671265 1106 4 0.025137932343419322 1106 5 0.1675944101399054 1106 6 0.03243798403080689 1106 7 -0.019927747552834774 1106 8 -0.02954156660196361 1106 9 0.09340351494853394 1106 10-11 -0.06642683714468234 1106 12-13 -0.15596535220584684 1106 14-15 -0.24203786994798548 1106 16-17 -0.16127834199917856 1106 18-19 -0.14089788680166038 1106 20-21 -0.22282235461705113 1106 22-23 -0.05865201045897095 1106 24-25 -0.10089854477742932 1106 26-27 -0.03713904446773597 1106 28-29 0.07541399564488671 1106 30-31 0.025352258677060036 1106 32-33 0.028497583628848133 1106 34-35 -0.13861967137483333 1106 36-37 -0.37596602200496676 1106 38-39 -0.16226510973926622 1106 40-41 0.0054009297324313366 1106 42-43 0.02560096373684928 1106 44-45 -0.05691967714683699 1106 46-47 -0.08879204896472714 1106 48-49 0.050353999128994076 1106 50-51 -0.003549927906981054 1106 52-53 0.07901865021870691 1106 54-55 0.07140392829872155 1106 56-57 0.023771632001555076 1106 58-59 -0.10175949575584298 1106 60-61 0.048983808279309926 1106 62-63 0.1909354703934909 1106 64-65 0.10422607602388467 1106 66-67 0.18868805121329757 1106 68-69 0.020098739214418515 1106 70-71 0.06930477809358848 1106 72-73 0.25380648735966815 1106 74-75 0.22981307725515165 1106 76-77 0.09603033798619975 1106 78-79 0.13849780196835226 1106 80-81 0.20138736181675299 1106 82-83 -0.1211424432091377 1106 84-85 0.2020837145501453 1106 86-87 0.13448834874604998 1106 88-89 0.27967192654873685 1106 90-91 0.24431812285920174 1106 92-93 0.24449324720042398 1106 94-95 0.4039837587373931 1106 96-97 0.6031098373603392 1106 98-99 0.7775774915183895 1106 100-101 0.8193267939593376 1107 1 -0.0921043668978534 1107 2 -0.08041707354223959 1107 3 -0.09899070119122655 1107 4 -0.06547560096787208 1107 5 0.04717939774995017 1107 6 0.09508142915987605 1107 7 -0.09432187815141191 1107 8 -0.0030548780398831354 1107 9 0.08050006015132993 1107 10-11 0.059319926285674285 1107 12-13 0.14492210270864092 1107 14-15 0.17002836104800423 1107 16-17 0.11804869997080658 1107 18-19 0.11496867899126784 1107 20-21 0.13320088441012956 1107 22-23 0.07607327511956896 1107 24-25 0.08985160018679039 1107 26-27 0.1317781201055226 1107 28-29 -0.014130101061198275 1107 30-31 0.027687696804633788 1107 32-33 -0.030113869450779873 1107 34-35 0.1300990378630118 1107 36-37 0.12926426372982291 1107 38-39 0.12401519916278403 1107 40-41 0.2272355304818916 1107 42-43 0.21136908503534357 1107 44-45 0.21569464396732485 1107 46-47 0.3050252803063884 1107 48-49 0.15090573119577755 1107 50-51 0.11120791144528397 1107 52-53 0.08038823894472102 1107 54-55 0.25642919238822515 1107 56-57 0.40333520773151577 1107 58-59 0.3778162712427786 1107 60-61 0.36234242949311835 1107 62-63 0.4145046263294603 1107 64-65 0.41852913475917575 1107 66-67 0.34594635063162826 1107 68-69 0.3052876209317219 1107 70-71 0.40247286058063736 1107 72-73 0.4200365574324536 1107 74-75 0.49693746947604467 1107 76-77 0.2254713258801928 1107 78-79 0.365456400651464 1107 80-81 0.35505116597867925 1107 82-83 0.44298247254186407 1107 84-85 0.5401647625671551 1107 86-87 0.48008535247171835 1107 88-89 0.3924868789082687 1107 90-91 0.4631143585349413 1107 92-93 0.5676257165225991 1107 94-95 0.5412393112622773 1107 96-97 0.651830352140518 1107 98-99 0.9822237537564327 1107 100-101 0.9120251885312918 1108 1 -0.12601207992049623 1108 2 -0.24462180880530227 1108 3 -0.11089560757374528 1108 4 -0.10930428222387434 1108 5 -0.010590824398747145 1108 6 -0.08247145403368705 1108 7 -0.12422754208404285 1108 8 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-0.709854869954853 2316 52-53 -0.7114243205130109 2316 54-55 -0.8503742609760607 2316 56-57 -0.9389960614215624 2316 58-59 -0.7926821706917266 2316 60-61 -0.8747146538956585 2316 62-63 -0.9471348875620436 2316 64-65 -0.9905544168754474 2316 66-67 -1.019516828775295 2316 68-69 -0.6740029677171435 2316 70-71 -0.7734733489210797 2316 72-73 -1.162398165680738 2316 74-75 -1.4042909594100301 2316 76-77 -0.8368113244795836 2316 78-79 -0.8505255877539035 2316 80-81 -0.9287737011041308 2316 82-83 -0.8312670940001397 2316 84-85 -0.9098579778406624 2316 86-87 -1.335107623224843 2316 88-89 -1.1158990188611853 2316 90-91 -1.0763546479773538 2316 92-93 -1.1398214194769523 2316 94-95 -1.117290625640539 2316 96-97 -1.3595165155420865 2316 98-99 -1.6134130670352498 2316 100-101 -1.8716385592865201 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5351.0 3 933.0 4 799.0 5 927.0 6 1041.0 7 1252.0 8 1507.0 9 1867.0 10 2377.0 11 2918.0 12 3733.0 13 4592.0 14 5575.0 15 6471.0 16 7903.0 17 9979.0 18 8816.0 19 6890.0 20 7122.0 21 7750.0 22 8317.0 23 9668.0 24 11013.0 25 12910.0 26 14897.0 27 18096.0 28 21970.0 29 26829.0 30 33143.0 31 42233.0 32 54622.0 33 72608.0 34 100450.0 35 144824.0 36 222272.0 37 331232.0 38 294906.0 39 40398.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.701487509070176 19.288768529076396 14.62546646625894 23.384277495594485 2 14.998377640058886 20.24446147040861 40.65368476477724 24.103476124755268 3 18.697891671022184 24.207215066653973 29.96990322010286 27.124990042220983 4 12.632043906906373 16.639054296843124 37.81329461124849 32.91560718500202 5 13.870365897173706 37.16219060379736 34.84043603809667 14.127007460932264 6 32.131558372296524 36.4185449254651 17.70313931994346 13.74675738229492 7 28.558307226264144 32.3919037878984 21.139217564663543 17.910571421173913 8 25.93627746694387 35.653368268705485 20.08391220934297 18.32644205500768 9 25.850438762842824 17.19854552609471 20.418426615474434 36.53258909558804 10-11 24.520217769913614 26.96406071431924 28.088577371853358 20.427144143913793 12-13 25.33319394624168 23.972810454808645 28.37483174025323 22.319163858696445 14-15 22.235633787046932 25.747184164659785 26.17540859788454 25.841773450408745 16-17 22.289657681169555 29.169207228269943 26.704247560089943 21.83688753047056 18-19 22.135759901755044 27.92680815536682 28.653743161927814 21.283688780950317 20-21 22.657041585102977 27.145797850634327 28.708616911764945 21.48854365249775 22-23 22.370186417411126 26.956450826849853 28.553486839848524 22.119875915890493 24-25 22.29352620788204 27.16288157268002 28.624908101559427 21.918684117878517 26-27 22.196095107444254 27.476803555703988 28.377962479866643 21.949138856985115 28-29 22.07230997757303 27.570153040585367 28.533735918421506 21.823801063420095 30-31 22.188164573205444 27.309207313343425 28.49084699334177 22.011781120109365 32-33 22.0391144804748 27.394020717185114 28.419102583272327 22.14776221906776 34-35 22.558268904729374 27.402104712675555 28.109651653515584 21.929974729079486 36-37 22.40038081763825 27.189110841944174 28.267878414066693 22.142629926350878 38-39 22.15049831736992 27.458063681076883 28.397780222624903 21.993657778928295 40-41 22.45476720082476 27.256430186134494 28.359316400170076 21.929486212870664 42-43 22.319634715016686 27.300205247913787 28.099516924782446 22.28064311228708 44-45 22.353113267661634 27.564993978997528 27.91295322970715 22.16893952363369 46-47 22.178842014893956 27.633778141358928 27.779300489572517 22.4080793541746 48-49 22.435658867565 27.692172268475158 27.636638539501764 22.23553032445808 50-51 22.374291785848598 27.718852979499736 27.669626715394518 22.237228519257147 52-53 22.61852436126408 27.68232189222098 27.48275029469663 22.216403451818316 54-55 22.37290329889066 27.545033053638328 27.619830564703406 22.462233082767604 56-57 22.30778365264367 27.927626225220166 27.547178236226554 22.21741188590961 58-59 22.505360953622088 27.580084299944225 27.658411811827616 22.25614293460607 60-61 22.496935881954773 27.47475257327675 27.92081309462333 22.10749845014515 62-63 22.349597457585986 27.594782382484212 27.934715715946968 22.12090444398283 64-65 22.278570460228924 27.504989968295217 27.783405771053232 22.43303380042262 66-67 22.123487742898735 27.74822502832221 27.587562626786582 22.540724601992473 68-69 22.159556025219487 27.596545165930785 27.797087062620975 22.446811746228757 70-71 22.20536034972027 27.58463922963174 27.701805874187425 22.508194546460572 72-73 22.224627747638596 27.48036638201642 27.7551464220668 22.539859448278182 74-75 22.076645392099632 27.633552809550128 27.65160879475328 22.63819300359696 76-77 22.239175211026957 27.546942070936126 27.634281424288364 22.579601293748556 78-79 22.135561354998732 27.534328802540823 27.66069050766341 22.669419334797038 80-81 22.097748735042636 27.753769634422632 27.51556484819541 22.632916782339326 82-83 22.14245356104778 27.574521404213208 27.665422081389274 22.61760295334973 84-85 22.237623269040434 27.334981504184004 27.49232455182468 22.93507067495088 86-87 22.301154058586583 27.279457420651394 27.676115513929307 22.743273006832716 88-89 22.233982655966866 27.001391405643282 27.75119725601864 23.013428682371213 90-91 22.023118469537124 27.15106474129239 27.980732020154562 22.84508476901592 92-93 22.059606827926544 26.976166976781812 28.023040207102984 22.941185988188657 94-95 21.831972637226446 27.09225228373669 27.964748101335456 23.11102697770141 96-97 21.862572053977186 27.194574454832694 27.85047394246683 23.09237954872329 98-99 21.774832114105134 27.20749767906022 27.69006547169683 23.327604735137815 100-101 21.71391908930577 27.186069618903687 28.037418858454977 23.062592433335567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1946.0 1 1096.0 2 219.0 3 169.0 4 125.0 5 140.5 6 207.5 7 331.5 8 518.0 9 639.0 10 645.5 11 667.5 12 756.5 13 853.5 14 955.0 15 1042.0 16 1098.0 17 1190.5 18 1307.0 19 1411.0 20 1575.0 21 1850.5 22 2285.5 23 2824.5 24 3431.5 25 4387.0 26 5912.0 27 8050.0 28 10342.5 29 13006.5 30 16621.0 31 20519.5 32 24375.0 33 28091.0 34 32419.0 35 36126.0 36 39523.5 37 43624.0 38 46954.5 39 49122.5 40 51106.5 41 54663.5 42 58047.0 43 60416.5 44 62341.0 45 63482.0 46 63042.0 47 62600.5 48 64649.0 49 64723.5 50 62836.5 51 60457.5 52 56301.5 53 52416.0 54 48899.0 55 44362.5 56 38806.0 57 33471.5 58 29260.5 59 25360.0 60 20737.0 61 16400.5 62 12764.5 63 9396.0 64 6862.5 65 5149.0 66 3733.0 67 2916.0 68 2128.0 69 1388.0 70 1058.0 71 808.5 72 620.0 73 485.5 74 343.0 75 244.5 76 177.0 77 113.5 78 68.0 79 45.5 80 30.5 81 22.0 82 15.0 83 11.5 84 11.0 85 7.0 86 8.5 87 8.0 88 3.0 89 2.5 90 3.0 91 3.0 92 2.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30202894207193404 2 0.26798932434911343 3 0.2697978808884663 4 0.27141266351288856 5 0.25959245470211784 6 0.2033334280672473 7 0.21160111510428914 8 0.20979255856493623 9 0.20475443677673888 10-11 0.21667153254497493 12-13 0.21557348036036783 14-15 0.1999100889034722 16-17 0.21069683683461268 18-19 0.19884433237135352 20-21 0.20252603675503617 22-23 0.20120191500301 24-25 0.19454901059039037 26-27 0.20575560200388066 28-29 0.20546494113148464 30-31 0.21098749770700867 32-33 0.21282834989885002 34-35 0.20278440197494374 36-37 0.20020074977586816 38-39 0.19351554971076015 40-41 0.19525951494513616 42-43 0.19322488883836414 44-45 0.20588478461383442 46-47 0.19845678454149218 48-49 0.20598167157129973 50-51 0.2124408020689887 52-53 0.21877074995672383 54-55 0.21692989776488247 56-57 0.18812217574518988 58-59 0.17840118434616806 60-61 0.18666887138320987 62-63 0.18686264529814053 64-65 0.1837945583117383 66-67 0.1957439497324628 68-69 0.18621673224837165 70-71 0.19125485403656903 72-73 0.20294588023738597 74-75 0.1939030975406215 76-77 0.19852137584646903 78-79 0.19561476712250903 80-81 0.1864750974682792 82-83 0.2004268193432873 84-85 0.1838591496167152 86-87 0.18799299313523612 88-89 0.19351554971076015 90-91 0.18976925402210057 92-93 0.19835989758402683 94-95 0.19577624538495128 96-97 0.1881867670501668 98-99 0.19535640190260148 100-101 0.1825027322122005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.131859878017444 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42734300423339 46.55850140257757 2 13.113934856590278 15.771305864918709 3 4.200289821834483 7.577137170408419 4 1.8574681697559108 4.467720628465598 5 0.9564922602818549 2.8757829284838343 6 0.5977030517636941 2.1564597694390715 7 0.3516963264512051 1.480370795524414 8 0.2555892924861712 1.2295247617680047 9 0.1815412075166111 0.982476940722745 >10 0.9628619689548025 10.385342620895457 >50 0.06342156784311813 2.6382773952194993 >100 0.03058173327266681 3.079036585898167 >500 6.460434094816074E-4 0.22454023645409374 >1k 4.3069560632107157E-4 0.5735228992244412 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2948 0.19041516707186945 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2264 0.14623471446767722 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2204 0.14235923616906385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.229565248844462E-5 0.0 22-23 0.0 0.0 0.0 1.2918260995377847E-4 0.0 24-25 0.0 0.0 0.0 1.937739149306677E-4 0.0 26-27 0.0 0.0 0.0 4.8443478732666925E-4 0.0 28-29 0.0 0.0 0.0 0.0012918260995377845 0.0 30-31 0.0 0.0 0.0 0.0032618609013329064 0.0 32-33 0.0 0.0 0.0 0.006329947887735145 0.0 34-35 0.0 0.0 0.0 0.009753287051510275 0.0 36-37 0.0 0.0 0.0 0.014306974052380964 0.0 38-39 0.0 0.0 0.0 0.021218243684908114 0.0 40-41 0.0 0.0 0.0 0.028840017672181043 0.0 42-43 0.0 0.0 0.0 0.0398851308232291 0.0 44-45 0.0 0.0 0.0 0.04996137439962382 0.0 46-47 0.0 0.0 0.0 0.07389245289356128 0.0 48-49 0.0 0.0 0.0 0.09979356618929386 0.0 50-51 0.0 0.0 0.0 0.11326085327697527 0.0 52-53 0.0 0.0 0.0 0.1383222796080083 0.0 54-55 0.0 0.0 0.0 0.17862725391358716 0.0 56-57 0.0 0.0 0.0 0.24883800242346577 0.0 58-59 0.0 0.0 0.0 0.3393950120010645 0.0 60-61 0.0 0.0 0.0 0.44161075212699163 0.0 62-63 0.0 0.0 0.0 0.564172753320639 0.0 64-65 0.0 0.0 0.0 0.7083405460290557 0.0 66-67 0.0 0.0 0.0 0.8974961826538759 0.0 68-69 0.0 0.0 0.0 1.1358380980185971 0.0 70-71 0.0 0.0 0.0 1.279069316804849 0.0 72-73 0.0 0.0 0.0 1.4509144836958627 0.0 74-75 0.0 0.0 0.0 1.6714937901919393 0.0 76-77 0.0 0.0 3.229565248844462E-5 1.9232706969918538 0.0 78-79 0.0 0.0 6.459130497688923E-5 2.1754351516216293 0.0 80-81 0.0 0.0 6.459130497688923E-5 2.533367868151061 0.0 82-83 0.0 0.0 6.459130497688923E-5 2.9327036111706786 0.0 84-85 0.0 0.0 6.459130497688923E-5 3.4641285728680344 0.0 86-87 0.0 3.229565248844462E-5 6.459130497688923E-5 4.192847675617299 0.0 88-89 0.0 6.459130497688923E-5 6.459130497688923E-5 4.98744990944299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2490 0.0 39.334698 1 GTATCAA 4025 0.0 36.973145 1 TCAACGC 4880 0.0 30.093847 4 ATCAACG 4915 0.0 29.975275 3 TATCAAC 5095 0.0 29.197067 2 CAACGCA 5105 0.0 28.765617 5 AACGCAG 5260 0.0 27.995615 6 GTACATG 4530 0.0 24.454887 1 ACGCAGA 5875 0.0 24.338104 7 CGCAGAG 5960 0.0 23.83082 8 TACATGG 4755 0.0 22.688951 2 ACATGGG 4600 0.0 21.902973 3 GCAGAGT 6610 0.0 21.054165 9 GAGTACT 3380 0.0 19.955591 12-13 CGTAACA 170 7.921632E-5 19.569881 2 CGCGGAA 110 1.349199E-6 19.427385 82-83 TGCACCG 295 2.9995135E-9 19.331705 5 AGAGTAC 5800 0.0 19.122814 10-11 CATGGGG 2615 0.0 19.08344 4 GTACTTT 3550 0.0 18.932762 14-15 >>END_MODULE