FastQCFastQC Report
Thu 26 May 2016
SRR937241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548196
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40000.2583652199075569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.20223537588264015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.13648142741616695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.037.399311
GTATCAA47800.035.0024381
ATCAACG58650.028.1037563
TATCAAC59650.027.9511432
TCAACGC59550.027.6790144
CAACGCA60700.027.2328745
AACGCAG62350.026.5858066
GTACCGT1000.001654630623.7482076
ACGCAGA69600.023.4092667
CGCAGAG70050.023.0554548
GTACATG45950.022.2400881
TACATGG46950.021.0441132
ACATGGG46950.020.4370733
GCAGAGT77250.020.2302079
AGTACTT45900.019.97319612-13
AGAGTAC68450.019.60414510-11
GAGTACT44050.019.4101812-13
TACGATG1250.006031322319.0004065
ATAACGC1250.006031322319.0004063
GTACTTT44750.018.73322114-15
Produced by FastQC (version 0.11.5)