##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937241_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548196 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2093404194301 33.0 31.0 34.0 31.0 34.0 2 32.442967815444554 34.0 31.0 34.0 31.0 34.0 3 32.53302230466944 34.0 31.0 34.0 31.0 34.0 4 36.128501171686274 37.0 35.0 37.0 35.0 37.0 5 36.01375601022093 37.0 35.0 37.0 35.0 37.0 6 36.083289195941596 37.0 35.0 37.0 35.0 37.0 7 36.03753336140902 37.0 35.0 37.0 35.0 37.0 8 36.011442995589704 37.0 35.0 37.0 35.0 37.0 9 37.73685889900245 39.0 37.0 39.0 35.0 39.0 10-11 37.658651746936435 39.0 37.0 39.0 35.0 39.0 12-13 37.584783838738765 39.0 37.0 39.0 35.0 39.0 14-15 38.856166790251365 40.0 38.0 41.0 35.0 41.0 16-17 38.82787644458453 40.0 38.0 41.0 35.0 41.0 18-19 38.795824301315854 40.0 38.0 41.0 35.0 41.0 20-21 38.685257874325984 40.0 38.0 41.0 34.0 41.0 22-23 38.565291797679365 40.0 38.0 41.0 34.0 41.0 24-25 38.433484197091325 40.0 38.0 41.0 34.0 41.0 26-27 38.39900697327728 40.0 38.0 41.0 34.0 41.0 28-29 38.317257634046335 40.0 38.0 41.0 34.0 41.0 30-31 38.44179677508532 40.0 38.0 41.0 34.0 41.0 32-33 38.418006505636235 40.0 38.0 41.0 34.0 41.0 34-35 38.328968037638646 40.0 38.0 41.0 34.0 41.0 36-37 38.19933522628918 40.0 38.0 41.0 34.0 41.0 38-39 38.093844707000926 40.0 38.0 41.0 33.5 41.0 40-41 37.961340166232176 40.0 38.0 41.0 33.0 41.0 42-43 37.79736867941785 40.0 37.0 41.0 33.0 41.0 44-45 37.56939689806717 40.0 37.0 41.0 33.0 41.0 46-47 37.31269264356709 40.0 37.0 41.0 32.0 41.0 48-49 37.09302665812339 40.0 37.0 41.0 31.5 41.0 50-51 36.91633908109826 40.0 36.5 41.0 31.0 41.0 52-53 36.707250244801045 39.0 36.0 41.0 31.0 41.0 54-55 36.49297924810554 39.0 36.0 41.0 31.0 41.0 56-57 36.2673401817341 39.0 35.0 40.0 30.0 41.0 58-59 36.04146794075169 39.0 35.0 40.0 30.0 41.0 60-61 35.73848724580092 38.0 35.0 40.0 29.0 41.0 62-63 35.38921137892102 38.0 34.5 40.0 28.5 41.0 64-65 35.04708060219765 37.5 34.0 40.0 28.0 41.0 66-67 34.637299153337175 37.0 34.0 39.5 28.0 41.0 68-69 34.24848791755049 36.0 34.0 39.0 27.0 40.5 70-71 33.852468615084916 36.0 33.0 39.0 26.0 40.0 72-73 33.4095857372064 35.0 33.0 38.0 26.0 40.0 74-75 32.9689767962196 35.0 32.5 37.5 26.0 39.5 76-77 31.582913274546634 34.0 30.5 36.0 24.5 38.5 78-79 32.24546956586892 35.0 32.0 36.0 26.0 39.0 80-81 32.10004224271346 35.0 32.0 36.0 25.0 37.5 82-83 31.778632033670156 35.0 32.0 36.0 25.0 37.0 84-85 31.41889269834052 34.0 31.5 35.0 24.0 37.0 86-87 31.053677958087995 34.0 31.0 35.0 23.0 36.0 88-89 30.673666964647886 34.0 31.0 35.0 20.5 36.0 90-91 30.26431666274813 34.0 31.0 35.0 19.5 35.5 92-93 29.929464357226088 34.0 30.0 35.0 18.0 35.0 94-95 29.539432022818815 34.0 30.0 35.0 10.5 35.0 96-97 29.097135633989495 34.0 29.5 35.0 2.0 35.0 98-99 28.616281142697694 34.0 29.0 35.0 2.0 35.0 100-101 26.716916333590838 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2613963129924599 1101 2 0.21671522547621436 1101 3 0.21724379078388267 1101 4 0.1602673348991459 1101 5 0.13195105311878308 1101 6 0.1710505577359669 1101 7 0.12683687872574723 1101 8 0.20567273704403277 1101 9 0.2599365846936337 1101 10-11 0.1974628640511824 1101 12-13 0.16441355603266317 1101 14-15 0.23940767831415855 1101 16-17 0.24381672881784056 1101 18-19 0.18576061035665248 1101 20-21 0.26992878210044324 1101 22-23 0.26959860965438764 1101 24-25 0.36683653001251315 1101 26-27 0.3231949266053036 1101 28-29 0.2519605534034426 1101 30-31 0.39570108495975376 1101 32-33 0.28986036751020805 1101 34-35 0.2978763803367457 1101 36-37 0.28062920909539457 1101 38-39 0.15844202117565942 1101 40-41 0.20362256916756394 1101 42-43 0.32553533198477425 1101 44-45 0.2856861737766252 1101 46-47 0.3432732962222147 1101 48-49 0.5068372528802243 1101 50-51 0.2900421468863925 1101 52-53 0.27007470504695164 1101 54-55 0.3080983413885292 1101 56-57 0.38929985023749225 1101 58-59 0.2932804406669689 1101 60-61 0.305051258133183 1101 62-63 0.3251386358468693 1101 64-65 0.3599242143414756 1101 66-67 0.38265771495061074 1101 68-69 0.46209198491938963 1101 70-71 0.2599721345992094 1101 72-73 0.1920769380409979 1101 74-75 0.19092913091927244 1101 76-77 0.23143482699767404 1101 78-79 0.237253730448316 1101 80-81 0.2885349356152034 1101 82-83 0.19890364348936984 1101 84-85 0.2309803555368859 1101 86-87 0.4156502512740232 1101 88-89 0.3185856307599444 1101 90-91 0.29341591475288453 1101 92-93 0.34638797710792346 1101 94-95 0.4159531846331639 1101 96-97 0.459809058455793 1101 98-99 0.5471725466406419 1101 100-101 1.0607797371215 1102 1 0.1618352654624715 1102 2 0.2018641897480009 1102 3 0.16772307215871507 1102 4 0.12271786679129093 1102 5 0.11008500789084508 1102 6 0.12986381767051824 1102 7 0.15651314112695758 1102 8 0.1748103409999331 1102 9 0.2533350615262151 1102 10-11 0.1776597384121672 1102 12-13 0.22850601309261975 1102 14-15 0.20141350033166816 1102 16-17 0.11484481899648102 1102 18-19 0.20917240141304205 1102 20-21 0.18081507769064586 1102 22-23 0.21870757458313506 1102 24-25 0.20276764401977232 1102 26-27 0.2081717282352784 1102 28-29 0.16263496206474315 1102 30-31 0.2532378976064038 1102 32-33 0.27666219421376326 1102 34-35 0.3137169135843152 1102 36-37 0.24398612612974802 1102 38-39 0.236600593815794 1102 40-41 0.3300474140109202 1102 42-43 0.31677884294757064 1102 44-45 0.2501847354181237 1102 46-47 0.39925340772817464 1102 48-49 0.4313766289255554 1102 50-51 0.3188171679081364 1102 52-53 0.32971356597602863 1102 54-55 0.30843403958935056 1102 56-57 0.2508371566853711 1102 58-59 0.3146808400034402 1102 60-61 0.27054978530244256 1102 62-63 0.12522629639317984 1102 64-65 0.36463499861007875 1102 66-67 0.27658583691229666 1102 68-69 0.45354990957174834 1102 70-71 0.39075672446354304 1102 72-73 0.3364647950790527 1102 74-75 0.32535929495476523 1102 76-77 0.3588629763025146 1102 78-79 0.3359599207050863 1102 80-81 0.404136401208568 1102 82-83 0.39194463783465494 1102 84-85 0.3176468020144121 1102 86-87 0.250660812231132 1102 88-89 0.344404793026893 1102 90-91 0.5270041982359359 1102 92-93 0.506847385510234 1102 94-95 0.5802335965890002 1102 96-97 0.5965818003394325 1102 98-99 0.6658100956394968 1102 100-101 1.196085694447035 1103 1 0.19312549580459404 1103 2 0.15622807569219077 1103 3 0.18100665877674516 1103 4 0.15000678420260982 1103 5 0.1102573070735744 1103 6 0.07158458450301453 1103 7 0.18695768956794012 1103 8 0.13459218118094896 1103 9 0.18281026616597273 1103 10-11 0.16725862334040187 1103 12-13 0.14534664922047114 1103 14-15 0.13435850158667506 1103 16-17 0.21661736752573546 1103 18-19 0.17136357995232032 1103 20-21 0.23435691081051857 1103 22-23 0.2005098755219521 1103 24-25 0.21730228452221212 1103 26-27 0.11720677006426428 1103 28-29 0.052511051921435126 1103 30-31 0.09337082725335932 1103 32-33 0.10651564796651058 1103 34-35 0.12629843160078735 1103 36-37 0.11791369243597671 1103 38-39 0.2361240587762623 1103 40-41 0.2493571507535819 1103 42-43 0.26912993609118274 1103 44-45 0.22938599906805734 1103 46-47 0.1984820149090183 1103 48-49 0.2940843372452946 1103 50-51 0.14822415163349945 1103 52-53 0.2960151925207697 1103 54-55 0.21580199028640834 1103 56-57 0.1338814092572207 1103 58-59 0.23388024642631677 1103 60-61 0.029217657021376908 1103 62-63 0.13844727055473527 1103 64-65 0.07488560473727546 1103 66-67 0.20552005521738437 1103 68-69 0.23441440279241021 1103 70-71 0.1344499215879722 1103 72-73 0.17978820174833743 1103 74-75 0.19118493811157578 1103 76-77 0.16606717841662544 1103 78-79 0.13059005209930064 1103 80-81 0.22827262973208917 1103 82-83 0.06836033673586073 1103 84-85 0.15469967690074427 1103 86-87 0.17509815058572187 1103 88-89 0.2969997161772291 1103 90-91 0.07610707482319867 1103 92-93 0.18024012559130753 1103 94-95 0.2543925807409728 1103 96-97 0.3720824931218978 1103 98-99 0.3354680624539732 1103 100-101 0.48822824489787564 1104 1 0.15550319492135856 1104 2 0.1227621839821893 1104 3 0.042240859809055564 1104 4 0.02133969995277596 1104 5 0.019995249359702427 1104 6 -0.07178628275894994 1104 7 -0.09933829920586845 1104 8 -0.030725109189880584 1104 9 -0.015466960499139759 1104 10-11 0.004835766974444766 1104 12-13 0.0161070875856808 1104 14-15 -0.06796636840415005 1104 16-17 -0.021075339821301498 1104 18-19 -0.04160868961218256 1104 20-21 -0.11672856263653131 1104 22-23 -0.11946613772416725 1104 24-25 -0.07269417087650254 1104 26-27 -0.0696820896909145 1104 28-29 -0.07974773310578342 1104 30-31 -0.03686704948191988 1104 32-33 -0.03904218972959228 1104 34-35 -0.06208450187237702 1104 36-37 -0.13031376414265594 1104 38-39 -0.16597114047402783 1104 40-41 -0.054453052842490024 1104 42-43 0.05617903124673518 1104 44-45 -0.0045343785048217455 1104 46-47 0.03805530318221173 1104 48-49 0.13671375160917876 1104 50-51 -0.08106930736323648 1104 52-53 0.07642762873008024 1104 54-55 0.16570414957981683 1104 56-57 0.196216378933876 1104 58-59 0.1781731955831347 1104 60-61 0.07866047483373251 1104 62-63 0.040570146828727616 1104 64-65 0.006210168824146933 1104 66-67 0.11334973955364092 1104 68-69 0.11685260291185529 1104 70-71 0.12530485028321436 1104 72-73 -0.022330304469285522 1104 74-75 -0.09967144726913091 1104 76-77 -0.036141156737560465 1104 78-79 -0.05347380899117127 1104 80-81 0.123455856484604 1104 82-83 0.19637385477691183 1104 84-85 0.14158562328501034 1104 86-87 0.10246829921619138 1104 88-89 0.10658664873754375 1104 90-91 0.019880472833364138 1104 92-93 0.06511390379638016 1104 94-95 0.04349948688672711 1104 96-97 -0.024466619028313374 1104 98-99 0.03604685214257941 1104 100-101 0.41543912925161663 1105 1 -0.0037717885429344733 1105 2 0.06246419975782658 1105 3 0.06722059329010222 1105 4 0.01807783376365535 1105 5 -0.03080017198787033 1105 6 0.039322551925693006 1105 7 0.05694267758343585 1105 8 0.07790014422064928 1105 9 0.07597987292841424 1105 10-11 0.03717076083441384 1105 12-13 0.041290308299139156 1105 14-15 0.12587475979280782 1105 16-17 0.14798751863681758 1105 18-19 0.10580826192396842 1105 20-21 0.007844575784112351 1105 22-23 0.040934032754869065 1105 24-25 -0.07683587589463059 1105 26-27 0.06986563495760123 1105 28-29 0.02252350816633708 1105 30-31 -0.006361417223438082 1105 32-33 -0.010694989165038749 1105 34-35 0.10154692950663957 1105 36-37 0.169271040963622 1105 38-39 0.13234443040946076 1105 40-41 0.20295184628020024 1105 42-43 0.03498537438261451 1105 44-45 0.14299626788647402 1105 46-47 0.26070018191824573 1105 48-49 0.24607644072646906 1105 50-51 0.19085615737554917 1105 52-53 0.28198501807362675 1105 54-55 0.2014966558368343 1105 56-57 0.3807995336309702 1105 58-59 0.38391219317139047 1105 60-61 0.3574282451878261 1105 62-63 0.40456795640059795 1105 64-65 0.3347736299029478 1105 66-67 0.2633017448956494 1105 68-69 0.31516311506987194 1105 70-71 0.303039881830685 1105 72-73 0.338989162897505 1105 74-75 0.31329925132821046 1105 76-77 0.34329398597540717 1105 78-79 0.37748369392075887 1105 80-81 0.36370712337916444 1105 82-83 0.4353697989437606 1105 84-85 0.39192677108452756 1105 86-87 0.3733792365600834 1105 88-89 0.3524265426054818 1105 90-91 0.3578095317966046 1105 92-93 0.28269626300888717 1105 94-95 0.18746373181503984 1105 96-97 0.35623535501283854 1105 98-99 0.43573713167510775 1105 100-101 0.736806047463407 1106 1 -0.2434985627669377 1106 2 -0.13730741537535351 1106 3 -0.07197120436286042 1106 4 0.04611106592160752 1106 5 -0.08821687996423577 1106 6 -0.15603011562418345 1106 7 -0.04545219344147 1106 8 -0.09355885397240371 1106 9 -0.2715775829700675 1106 10-11 -0.3174841832834048 1106 12-13 -0.41859585459473436 1106 14-15 -0.31519577859420167 1106 16-17 -0.12564235505129773 1106 18-19 -0.21978247838648457 1106 20-21 -0.20068516005792958 1106 22-23 -0.15669272938102807 1106 24-25 -0.18440128137009282 1106 26-27 -0.09200388120160596 1106 28-29 -0.04927089110935157 1106 30-31 0.013578887793677552 1106 32-33 -0.13300769598206585 1106 34-35 -0.13288633915945525 1106 36-37 0.02891310795116908 1106 38-39 -0.00813682207042632 1106 40-41 -0.177264192705465 1106 42-43 -0.5651258221541298 1106 44-45 -0.10622209918959413 1106 46-47 -0.010045444508072876 1106 48-49 -0.02350357288047178 1106 50-51 -0.007993522812483889 1106 52-53 0.13412654423215997 1106 54-55 0.0506126747221316 1106 56-57 0.158679748624877 1106 58-59 0.094850838949462 1106 60-61 0.15943779513099088 1106 62-63 0.26919157249663783 1106 64-65 0.1516639030973792 1106 66-67 -0.046514206024589555 1106 68-69 0.19507242300729644 1106 70-71 0.08748877444625691 1106 72-73 0.12903271835800467 1106 74-75 0.00798533186976158 1106 76-77 0.06266486160981799 1106 78-79 0.12214899478367869 1106 80-81 0.08343141031220824 1106 82-83 0.009421397341718318 1106 84-85 -0.19915946092397974 1106 86-87 -0.010066100344172213 1106 88-89 -0.1634088614382101 1106 90-91 -0.19400328179278148 1106 92-93 -0.2035264442853908 1106 94-95 -0.7663595722897263 1106 96-97 -0.35273296616679417 1106 98-99 -0.16483581614438592 1106 100-101 0.1087154404006867 1107 1 -0.08322427465407856 1107 2 -0.013512412329461654 1107 3 -0.07792177802763689 1107 4 0.01308931723843898 1107 5 -0.07909001785404257 1107 6 0.05391433308549409 1107 7 -0.05477928556540945 1107 8 0.002860192338602019 1107 9 -0.03845699241884404 1107 10-11 -0.14913780927920328 1107 12-13 -0.08189635509260995 1107 14-15 -0.13262709371959858 1107 16-17 -0.17984527563174169 1107 18-19 -0.02498915318323469 1107 20-21 -0.027474817078193325 1107 22-23 0.02878121969110481 1107 24-25 -0.1440775028792629 1107 26-27 -0.047721219693336536 1107 28-29 -0.05026143168795727 1107 30-31 -0.18966692174706878 1107 32-33 -0.18149595937827456 1107 34-35 0.03275442196151346 1107 36-37 -0.19639021737167184 1107 38-39 -0.031566627176019324 1107 40-41 0.008280164801170997 1107 42-43 -0.020287223362991824 1107 44-45 -0.2067655365640917 1107 46-47 -0.047436567026856835 1107 48-49 -0.07912102784511887 1107 50-51 -0.05562878320216669 1107 52-53 -0.0027088724835380162 1107 54-55 -0.13730994467225344 1107 56-57 -0.07857545668033339 1107 58-59 -0.014198708912651625 1107 60-61 0.06429786753015065 1107 62-63 -0.0272744256306936 1107 64-65 0.09276271448725737 1107 66-67 0.23637646723050665 1107 68-69 0.15900594355633046 1107 70-71 0.12858183742149265 1107 72-73 0.2014318790123042 1107 74-75 0.06871501787900058 1107 76-77 0.10423187944417478 1107 78-79 0.09573310045575312 1107 80-81 0.20594343665152337 1107 82-83 0.3053926074292974 1107 84-85 0.32947420264098426 1107 86-87 0.373427809229792 1107 88-89 0.2501600591599811 1107 90-91 0.21238571458417255 1107 92-93 0.3387236318135898 1107 94-95 0.39406189838622296 1107 96-97 0.2979588304596952 1107 98-99 0.4141670769671215 1107 100-101 1.0058934703456828 1108 1 -0.102621964828856 1108 2 -0.105070583043954 1108 3 -0.18636034971991222 1108 4 -0.0684447281238647 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-0.1657475240020787 2316 76-77 -0.17900759591773507 2316 78-79 -0.32763495254826935 2316 80-81 -0.3106990166114727 2316 82-83 -0.5168526255258215 2316 84-85 -0.5871002776562868 2316 86-87 -0.5261191700694852 2316 88-89 -0.3918522981166497 2316 90-91 -0.46842858069157955 2316 92-93 -0.3863964752748643 2316 94-95 -0.27486184478420483 2316 96-97 -0.3176890526963163 2316 98-99 -0.4886268651471184 2316 100-101 -0.8030900830764018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 5.0 5 31.0 6 60.0 7 109.0 8 259.0 9 543.0 10 942.0 11 1495.0 12 2260.0 13 3312.0 14 4167.0 15 5640.0 16 7511.0 17 10217.0 18 9148.0 19 5985.0 20 5297.0 21 5666.0 22 5796.0 23 6453.0 24 7859.0 25 9326.0 26 11848.0 27 14785.0 28 18974.0 29 24009.0 30 30747.0 31 39602.0 32 51257.0 33 68863.0 34 95878.0 35 139147.0 36 222279.0 37 367902.0 38 336890.0 39 33928.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89434595360332 19.68840961849663 15.280362839224686 23.13688158867536 2 15.390816149893165 20.53809724350147 39.878154962291596 24.19293164431377 3 18.92809437564753 24.19583825303773 30.37012109577857 26.505946275536175 4 13.06145991851161 17.160424132345 37.564623600629375 32.21349234851401 5 13.804515863535672 37.22160239443744 34.80765491262151 14.166226829405376 6 32.28893499272702 36.65550098307966 17.377450917067346 13.678113107125972 7 28.58546333926712 32.472891029301195 21.0947451097923 17.846900521639377 8 26.146754028559695 35.45177742353036 19.984291394629622 18.417177153280335 9 25.943549783102398 16.829199920423513 20.399355120411112 36.82789517606298 10-11 24.489276551547736 26.884580505310694 28.08781963007268 20.538323313068886 12-13 25.376298453724615 23.848303076047088 28.61043852960279 22.164959940625508 14-15 22.35136894811768 25.508010613643233 26.389585039620307 25.751035398618775 16-17 22.12920715464967 28.8510304896796 27.078871150681177 21.940891204989548 18-19 21.92387139612814 27.571218372867516 29.24513433699609 21.25977589400825 20-21 22.45474733173319 26.600540241674825 29.523329087531554 21.42138333906043 22-23 22.23678771202315 26.48848971049878 29.27350178919764 22.00122078828043 24-25 22.2137105043462 26.749419080637583 29.310502408453733 21.726368006562492 26-27 22.142723575410912 27.05392706054776 29.044497244981105 21.758852119060222 28-29 21.915787685467752 27.105929574494404 29.276363703305858 21.70191903673199 30-31 22.073077310624754 26.923852018736643 29.173825536301607 21.829245134336997 32-33 22.029575066722817 26.89924273154045 29.02132546525117 22.049856736485562 34-35 22.40659451387292 26.84049693966397 28.91497588160672 21.83793266485639 36-37 22.316909486912508 26.74519246917057 29.00117297809838 21.936725065818543 38-39 22.142642146084864 27.01282654134231 29.044255378517963 21.80027593405486 40-41 22.299631312831192 26.787047634795595 29.079166978857973 21.83415407351524 42-43 22.205350581813192 26.854203509451942 28.841008745366892 22.099437163367977 44-45 22.246246599267792 27.03275295892768 28.53043154742681 22.190568894377716 46-47 22.173645972473768 27.15150407312769 28.36791982410496 22.30693013029358 48-49 22.352467000302287 27.329711483558928 28.25239827515379 22.06542324098499 50-51 22.458429036116875 27.227043604298164 28.054102968874744 22.26042439071022 52-53 22.631339959540007 27.28110652656382 27.95295298528093 22.134600528615238 54-55 22.40472136602859 27.14682120351687 28.155737387255876 22.292720043198667 56-57 22.351853382905006 27.408868127808105 28.06217688199685 22.177101607290034 58-59 22.438857870708876 27.138456629522363 28.11271957814127 22.309965921627494 60-61 22.37048797439084 27.104933742239357 28.44966012055321 22.074918162816594 62-63 22.332508287064428 27.154572160114093 28.389138067789865 22.12378148503161 64-65 22.26026291244778 26.982662401918105 28.29028107552274 22.46679361011138 66-67 22.03374120589383 27.418201571377267 28.15541443073099 22.392642791997915 68-69 22.189147885668223 27.08807541164039 28.321510971479064 22.401265731212327 70-71 22.15165263312914 27.199237047505616 28.167428413456697 22.481681905908555 72-73 22.1407832992614 27.04885366507449 28.26029666805538 22.550066367608732 74-75 22.085769502052713 27.178212577735632 27.981599229038185 22.75441869117347 76-77 22.21805249464538 27.12954302943555 28.04153996005674 22.610864515862332 78-79 22.063937640970526 27.1308025598826 28.10106084759294 22.704198951553938 80-81 22.00690351867593 27.423013623598045 27.87760722802539 22.69247562970063 82-83 22.042116196107333 27.171289367669278 28.064201297900198 22.722393138323195 84-85 22.075605574159844 27.015762707102116 27.900576128284865 23.008055590453175 86-87 22.074239954114336 26.8747949226067 27.949981785252 23.100983338026968 88-89 21.973122266173018 26.7227469906911 28.038730238290242 23.26540050484564 90-91 21.75408190422544 26.797840124233552 28.399142922847552 23.048935048693455 92-93 21.708210568334525 26.494858645449515 28.42202287005755 23.374907916158406 94-95 21.41185612157634 26.664647111864387 28.461544920668956 23.461951845890315 96-97 21.561223514335396 26.710248573178074 28.338014049900657 23.390513862585873 98-99 21.328812368213427 26.631311505111277 28.215350203091404 23.82452592358389 100-101 20.83783510545694 26.8081135580295 28.439248928376358 23.914802408137202 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 68.0 1 55.0 2 62.5 3 89.0 4 88.0 5 93.5 6 159.0 7 341.5 8 558.0 9 692.5 10 711.5 11 722.5 12 853.5 13 992.5 14 1093.5 15 1166.0 16 1239.5 17 1398.5 18 1548.5 19 1631.0 20 1797.0 21 2096.5 22 2555.5 23 3092.0 24 3757.5 25 4748.0 26 6220.0 27 8443.0 28 10663.5 29 13338.0 30 16818.5 31 20436.0 32 24278.0 33 27854.0 34 32319.0 35 35943.0 36 39262.5 37 43303.0 38 47080.5 39 49104.5 40 50760.5 41 54186.0 42 57587.5 43 60451.5 44 61976.0 45 63268.0 46 63050.0 47 62566.0 48 64533.5 49 64522.5 50 63088.0 51 61231.5 52 56593.0 53 52471.0 54 48958.0 55 44170.5 56 38749.0 57 33524.0 58 29256.0 59 25243.5 60 20972.0 61 16818.5 62 12982.0 63 9416.5 64 6625.0 65 4890.5 66 3707.0 67 2923.0 68 2059.0 69 1328.0 70 971.0 71 728.5 72 557.0 73 415.0 74 316.0 75 248.0 76 166.0 77 93.0 78 53.0 79 36.5 80 19.0 81 14.0 82 10.0 83 6.5 84 5.5 85 3.0 86 1.5 87 0.5 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05451506140049451 2 0.0 3 0.0 4 0.0 5 0.017762608868644537 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0036494087311942417 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010334608796302277 24-25 2.260695674191123E-4 26-27 0.0013887130570031186 28-29 0.003197269596356017 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015082069712103635 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.459130497688923E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.937739149306677E-4 84-85 0.0010657565321186724 86-87 0.0 88-89 0.0 90-91 0.00930114791667205 92-93 9.365739221648938E-4 94-95 0.0 96-97 0.0 98-99 9.688695746533384E-5 100-101 0.012563008818004956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.475322238818286 #Duplication Level Percentage of deduplicated Percentage of total 1 76.36439153348546 44.654324024918495 2 13.523874811817574 15.816258750769412 3 4.458826122511734 7.821938829622028 4 1.987726546183076 4.649318012428347 5 1.033475349245958 3.0216402036516334 6 0.6214611733027892 2.1804085420676813 7 0.39995067450436467 1.637107119989281 8 0.28969046408943266 1.3551794589713915 9 0.19975502286672625 1.05126653978589 >10 1.0172066517707092 10.708541663707608 >50 0.06969126735374379 2.830515941502255 >100 0.033065729557623096 3.4269977853993048 >500 5.529083192920589E-4 0.2487585005184398 >1k 3.317449915752353E-4 0.597744626668172 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4000 0.2583652199075569 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.20223537588264015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2113 0.13648142741616695 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.229565248844462E-5 0.0 22-23 0.0 0.0 0.0 1.2918260995377847E-4 0.0 24-25 0.0 0.0 0.0 1.937739149306677E-4 0.0 26-27 0.0 0.0 0.0 4.8443478732666925E-4 0.0 28-29 0.0 0.0 0.0 0.0012918260995377845 0.0 30-31 0.0 0.0 0.0 0.0032618609013329064 0.0 32-33 0.0 0.0 0.0 0.006362243540223589 0.0 34-35 0.0 0.0 0.0 0.010011652271417832 0.0 36-37 0.0 0.0 0.0 0.014759113187219189 0.0 38-39 0.0 0.0 0.0 0.02176726977721167 0.0 40-41 0.0 0.0 0.0 0.029421339416973044 0.0 42-43 0.0 0.0 0.0 0.040531043872997996 0.0 44-45 0.0 0.0 0.0 0.05034892222948516 0.0 46-47 0.0 0.0 0.0 0.07424770507093417 0.0 48-49 0.0 0.0 0.0 0.10043947923906275 0.0 50-51 0.0 0.0 0.0 0.11403594893669794 0.0 52-53 0.0 0.0 0.0 0.13945262744510384 0.0 54-55 0.0 0.0 0.0 0.17985448870814807 0.0 56-57 0.0 0.0 0.0 0.25077574157277244 0.0 58-59 0.0 0.0 0.0 0.34207555115760535 0.0 60-61 0.0 0.0 0.0 0.44471113476588237 0.0 62-63 0.0 0.0 0.0 0.5685972577115559 0.0 64-65 0.0 0.0 0.0 0.7130557112923687 0.0 66-67 0.0 0.0 0.0 0.9022759392221656 0.0 68-69 0.0 0.0 0.0 1.1411991763316789 0.0 70-71 0.0 0.0 0.0 1.2851086038201882 0.0 72-73 0.0 0.0 0.0 1.4575673881084823 0.0 74-75 0.0 0.0 0.0 1.6792447467891662 0.0 76-77 0.0 0.0 0.0 1.9321520014261762 0.0 78-79 0.0 0.0 0.0 2.186674038687608 0.0 80-81 0.0 0.0 0.0 2.547319590026069 0.0 82-83 0.0 0.0 0.0 2.9512736113515343 0.0 84-85 0.0 0.0 0.0 3.4881565383194375 0.0 86-87 0.0 0.0 0.0 4.226532041162747 0.0 88-89 0.0 0.0 0.0 5.031371996827275 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2885 0.0 37.39931 1 GTATCAA 4780 0.0 35.002438 1 ATCAACG 5865 0.0 28.103756 3 TATCAAC 5965 0.0 27.951143 2 TCAACGC 5955 0.0 27.679014 4 CAACGCA 6070 0.0 27.232874 5 AACGCAG 6235 0.0 26.585806 6 GTACCGT 100 0.0016546306 23.748207 6 ACGCAGA 6960 0.0 23.409266 7 CGCAGAG 7005 0.0 23.055454 8 GTACATG 4595 0.0 22.240088 1 TACATGG 4695 0.0 21.044113 2 ACATGGG 4695 0.0 20.437073 3 GCAGAGT 7725 0.0 20.230207 9 AGTACTT 4590 0.0 19.973196 12-13 AGAGTAC 6845 0.0 19.604145 10-11 GAGTACT 4405 0.0 19.41018 12-13 TACGATG 125 0.0060313223 19.000406 5 ATAACGC 125 0.0060313223 19.000406 3 GTACTTT 4475 0.0 18.733221 14-15 >>END_MODULE