FastQCFastQC Report
Thu 26 May 2016
SRR937240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.17255275384237173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27500.14262701324512242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23980.12437075554974673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49850.036.699421
GGTATCA31300.035.8287241
ATCAACG59700.030.3984243
TCAACGC60450.030.1014274
CAACGCA60900.029.9570185
TATCAAC62600.028.9901922
AACGCAG63700.028.78646
ACGCAGA72650.025.043947
CGCAGAG74100.024.5519628
GTACATG60250.023.502981
TACATGG60600.022.656212
GTATAGG5200.021.9316641
GTGTAGC10000.021.3833711
GCAGAGT82900.021.2575749
ACATGGG60650.021.2275833
GAGTACT39650.020.36520412-13
CATGGGG33800.019.2569964
GTACTTT43400.018.7694614-15
AGAGTAC71450.018.6804410-11
AGTACTT43300.018.42911112-13
Produced by FastQC (version 0.11.5)