##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1928106 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85857260959719 33.0 31.0 34.0 30.0 34.0 2 31.964488466920386 33.0 31.0 34.0 30.0 34.0 3 32.02689426826119 34.0 31.0 34.0 30.0 34.0 4 35.62384329492258 37.0 35.0 37.0 33.0 37.0 5 35.635323991523286 37.0 35.0 37.0 33.0 37.0 6 35.65435614017072 37.0 35.0 37.0 33.0 37.0 7 35.536232447801105 37.0 35.0 37.0 33.0 37.0 8 35.54639682673048 37.0 35.0 37.0 33.0 37.0 9 37.20538238042929 39.0 37.0 39.0 34.0 39.0 10-11 37.09452488607992 39.0 37.0 39.0 33.0 39.0 12-13 36.96198315860228 39.0 37.0 39.0 33.0 39.0 14-15 38.200610599209796 40.0 38.0 41.0 33.0 41.0 16-17 38.13110456582781 40.0 38.0 41.0 33.0 41.0 18-19 38.06760572292187 40.0 38.0 41.0 33.0 41.0 20-21 37.939509809108 40.0 38.0 41.0 33.0 41.0 22-23 37.79342084926866 40.0 37.5 41.0 33.0 41.0 24-25 37.74808957598805 40.0 37.5 41.0 32.5 41.0 26-27 37.78560670419573 40.0 38.0 41.0 33.0 41.0 28-29 37.71266154454164 40.0 37.5 41.0 33.0 41.0 30-31 37.541430035485604 40.0 37.0 41.0 32.0 41.0 32-33 37.36516405218386 40.0 37.0 41.0 32.0 41.0 34-35 37.457014552104496 40.0 37.0 41.0 32.0 41.0 36-37 37.37351992058528 40.0 37.0 41.0 32.0 41.0 38-39 37.295235842842665 40.0 37.0 41.0 31.5 41.0 40-41 37.11778605533098 40.0 37.0 41.0 31.0 41.0 42-43 36.91369561631985 40.0 36.0 41.0 31.0 41.0 44-45 36.760901890248775 39.0 36.0 41.0 30.5 41.0 46-47 36.53406166465952 39.0 36.0 41.0 30.0 41.0 48-49 36.3109038092304 39.0 36.0 41.0 30.0 41.0 50-51 35.36265511335995 38.0 34.0 40.0 28.0 40.5 52-53 35.48728570939565 38.5 35.0 39.5 28.5 40.5 54-55 35.890820836613756 39.0 35.0 40.0 29.0 41.0 56-57 35.69917784603129 39.0 35.0 40.0 28.0 41.0 58-59 35.45235324198981 38.5 35.0 40.0 28.0 41.0 60-61 35.1660502586476 38.0 34.5 40.0 27.5 41.0 62-63 34.86781095022784 38.0 34.0 40.0 26.5 41.0 64-65 34.4546614657078 37.0 34.0 40.0 26.0 41.0 66-67 34.110846602832 37.0 34.0 39.5 26.0 41.0 68-69 33.658043696767706 36.0 33.0 39.0 25.5 41.0 70-71 33.193932543127815 36.0 32.5 39.0 24.5 40.0 72-73 32.743953392603935 35.0 32.0 38.0 23.0 40.0 74-75 32.26658544706567 35.0 32.0 37.5 22.0 39.5 76-77 31.78799376175376 35.0 31.0 37.0 20.5 39.0 78-79 31.284994445326138 35.0 31.0 36.0 19.5 39.0 80-81 30.800035630821128 34.0 30.5 36.0 18.0 37.5 82-83 30.334002643008215 34.0 30.0 35.0 15.0 37.0 84-85 29.826165936935002 34.0 29.5 35.0 8.5 36.5 86-87 29.38420501777392 34.0 29.0 35.0 7.0 36.0 88-89 28.953101904148426 34.0 29.0 35.0 2.0 36.0 90-91 28.475854024623125 34.0 29.0 35.0 2.0 35.0 92-93 27.997477317118456 33.0 27.0 35.0 2.0 35.0 94-95 27.481508537393694 33.0 26.5 35.0 2.0 35.0 96-97 26.77460367842847 33.0 25.5 35.0 2.0 35.0 98-99 25.871227256177825 32.0 24.5 34.5 2.0 35.0 100-101 23.26018460603307 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0012254020752173744 1101 2 -0.07926655235522162 1101 3 -0.039442137395873544 1101 4 -0.05144695846485092 1101 5 -0.05083822375959812 1101 6 -0.0201430014611077 1101 7 -0.04359385502388591 1101 8 -0.10384103710210724 1101 9 -0.1171480824042348 1101 10-11 -0.18896631612592074 1101 12-13 -0.08372290537227656 1101 14-15 -0.1784844625057005 1101 16-17 -0.28615238181483704 1101 18-19 -0.12290960829886899 1101 20-21 -0.009746764320119894 1101 22-23 -0.08083527156576054 1101 24-25 -0.06833172312327918 1101 26-27 -0.11047192884453949 1101 28-29 -0.19514381012279358 1101 30-31 -0.19434178061236906 1101 32-33 -0.3108499457497018 1101 34-35 -0.30801061646110384 1101 36-37 -0.4123566177033027 1101 38-39 -0.45647317562313106 1101 40-41 -0.3389361162522775 1101 42-43 -0.28860849672241784 1101 44-45 -0.26762896961638916 1101 46-47 -0.2967068686235379 1101 48-49 -0.29136844695054265 1101 50-51 -0.3391851481363872 1101 52-53 -0.3113413013273316 1101 54-55 -0.35884001849391467 1101 56-57 -0.3293092267592783 1101 58-59 -0.3398789193457077 1101 60-61 -0.27432846376558473 1101 62-63 -0.35912302139811914 1101 64-65 -0.356666936030237 1101 66-67 -0.4564756239673997 1101 68-69 -0.24375418709249175 1101 70-71 -0.3142864056735064 1101 72-73 -0.3896251113062803 1101 74-75 -0.25454727401636035 1101 76-77 -0.21519951111500646 1101 78-79 -0.3855762976009025 1101 80-81 -0.32472768748465697 1101 82-83 -0.45988836919833176 1101 84-85 -0.3329315458777202 1101 86-87 -0.25639711518815034 1101 88-89 -0.48707887527289273 1101 90-91 -0.5513820781710521 1101 92-93 -0.3868856247896524 1101 94-95 -0.4395395348680502 1101 96-97 -0.0863004810421728 1101 98-99 -0.14349683632541854 1101 100-101 0.12002730810193185 1102 1 -0.10576480946410172 1102 2 0.01753149751130323 1102 3 -0.07303263685130545 1102 4 -0.11505219730123173 1102 5 -0.05728541451604485 1102 6 -0.08609485968818831 1102 7 -0.13390004934321098 1102 8 -0.12928107118477783 1102 9 -0.15794765087364482 1102 10-11 -0.08649626730386473 1102 12-13 -0.011492637761463698 1102 14-15 0.05663099597838084 1102 16-17 -0.008028595065162847 1102 18-19 -0.06919504379775532 1102 20-21 -0.03198682382736706 1102 22-23 -0.21697312428876359 1102 24-25 -0.06736369967769917 1102 26-27 -0.14580349620076305 1102 28-29 -0.22859902679856958 1102 30-31 -0.23153810298220634 1102 32-33 -0.3334340100395181 1102 34-35 -0.13563458702356002 1102 36-37 -0.2369579844459011 1102 38-39 -0.11892417319405268 1102 40-41 -0.22879102439741672 1102 42-43 -0.258282821682819 1102 44-45 -0.12499926134618988 1102 46-47 -0.17973622369832043 1102 48-49 -0.22526197983241047 1102 50-51 -0.36526680911688203 1102 52-53 -0.3543590779140402 1102 54-55 -0.27990874386385656 1102 56-57 -0.284518601555277 1102 58-59 -0.3052267721717854 1102 60-61 -0.19016539605032534 1102 62-63 -0.24327372040209383 1102 64-65 -0.3452628124393229 1102 66-67 -0.5617453821247338 1102 68-69 -0.5690203720665679 1102 70-71 -0.6485834677610072 1102 72-73 -0.6417694507312035 1102 74-75 -0.7433999729722416 1102 76-77 -0.6520795724088444 1102 78-79 -0.6736929723415912 1102 80-81 -0.683521239950327 1102 82-83 -0.6754496681878734 1102 84-85 -0.6153787984777281 1102 86-87 -0.656375899412545 1102 88-89 -0.938099208059942 1102 90-91 -0.7938272864979403 1102 92-93 -0.7471934854492481 1102 94-95 -0.9206192718267907 1102 96-97 -0.6903428163922953 1102 98-99 -0.6726144146691801 1102 100-101 -0.4315678261844802 1103 1 0.05444323501807702 1103 2 -0.010086255235695063 1103 3 -0.059833258799336875 1103 4 -0.04705797969823777 1103 5 0.008776859105260826 1103 6 0.06553632083079464 1103 7 0.1764980547184365 1103 8 0.19101320768152874 1103 9 0.0967088983895863 1103 10-11 0.07825323381212712 1103 12-13 0.06024858385051601 1103 14-15 -0.03959181618686358 1103 16-17 -0.034373163588718114 1103 18-19 -0.07172439465741576 1103 20-21 -0.09589726832674472 1103 22-23 -0.06016008845868015 1103 24-25 -0.06334486210429446 1103 26-27 -0.03042604611602684 1103 28-29 -0.061398993121663636 1103 30-31 -0.14193961056130178 1103 32-33 -0.1349363942930566 1103 34-35 -0.10387260395516762 1103 36-37 -0.01348213329784187 1103 38-39 -0.1209940491985364 1103 40-41 -0.07473528552960573 1103 42-43 -0.07623404748650131 1103 44-45 -0.2504175357270171 1103 46-47 -0.03848711488249279 1103 48-49 -0.050230721211661944 1103 50-51 -0.14163511269630646 1103 52-53 -0.16051039829967806 1103 54-55 0.004598444723256989 1103 56-57 -0.05088817697543391 1103 58-59 -0.18207407604857195 1103 60-61 -0.1471323962617035 1103 62-63 -0.11442550653222128 1103 64-65 -9.513440185955346E-4 1103 66-67 0.057401015699880986 1103 68-69 0.18979115546788705 1103 70-71 -0.03861940173992906 1103 72-73 -0.09424873405791345 1103 74-75 -0.006675482335495531 1103 76-77 0.040594445855507644 1103 78-79 0.014270309079147836 1103 80-81 0.0909979684273452 1103 82-83 -0.15525203130377463 1103 84-85 -0.1285369760553543 1103 86-87 0.07533780744787322 1103 88-89 0.057907480747502404 1103 90-91 0.22050555242969594 1103 92-93 0.04035850775804661 1103 94-95 -0.2855645647621614 1103 96-97 -0.1781367280369821 1103 98-99 0.3390126278891188 1103 100-101 0.6758395712711227 1104 1 -0.05174950980858739 1104 2 -0.011719892545642097 1104 3 -0.11317316459999205 1104 4 -0.09942104351527092 1104 5 0.022840855797355175 1104 6 -0.038773159321351613 1104 7 -0.07605983580845077 1104 8 -0.0124646928485177 1104 9 -0.036430962451056814 1104 10-11 0.02247099734955782 1104 12-13 -0.029659473831920025 1104 14-15 -0.030190163500229517 1104 16-17 -0.10422436220633813 1104 18-19 -0.020885727965470835 1104 20-21 -0.023082591140521913 1104 22-23 -0.1991339168577042 1104 24-25 -0.16880474974038862 1104 26-27 -0.05395353807875125 1104 28-29 -0.027639487645657823 1104 30-31 -0.19730993930203766 1104 32-33 -0.25295732326797093 1104 34-35 -0.1916823021362717 1104 36-37 -0.1130507248487902 1104 38-39 -0.08469944918463312 1104 40-41 0.04605027779314241 1104 42-43 0.0418473698552404 1104 44-45 -0.05704706073083088 1104 46-47 -0.046688254049968236 1104 48-49 -0.11721504208529154 1104 50-51 -0.18346688100600517 1104 52-53 -0.18166417327032036 1104 54-55 -0.27416535362116434 1104 56-57 -0.322169022656972 1104 58-59 -0.22736767484457943 1104 60-61 -0.19873619565338174 1104 62-63 -0.06333766388142692 1104 64-65 0.07854504067297796 1104 66-67 -0.03507847468111436 1104 68-69 0.00805791999931671 1104 70-71 0.04980556244102985 1104 72-73 0.08181710256391739 1104 74-75 -0.05488064804202253 1104 76-77 -0.16540184720069817 1104 78-79 -0.034563664798906046 1104 80-81 0.08794939988003136 1104 82-83 0.040280677103474716 1104 84-85 -0.023793695003831772 1104 86-87 0.12300512323596635 1104 88-89 -0.06565445347185062 1104 90-91 0.13963937707302065 1104 92-93 0.3183935115634 1104 94-95 0.3793077641573248 1104 96-97 0.3670708779688816 1104 98-99 0.46100840995404724 1104 100-101 0.528194690347437 1105 1 -0.13317212866708772 1105 2 -0.04627337666509135 1105 3 -0.2034406705101155 1105 4 -0.08446951888574006 1105 5 -0.20841266703649097 1105 6 -0.20664935679067753 1105 7 -0.09995347476196059 1105 8 -0.19961527774189847 1105 9 -0.2320426734980785 1105 10-11 -0.23191283474056235 1105 12-13 -0.19994524552522108 1105 14-15 -0.3303014085623843 1105 16-17 -0.2808465279063199 1105 18-19 -0.2290446111550679 1105 20-21 -0.25508061600010024 1105 22-23 -0.24004409356716394 1105 24-25 -0.20651885566721973 1105 26-27 -0.2563059967961365 1105 28-29 -0.36894494249278154 1105 30-31 -0.3304306964568511 1105 32-33 -0.4185286754360362 1105 34-35 -0.40501916271619365 1105 36-37 -0.33938316504445254 1105 38-39 -0.121548806043144 1105 40-41 -0.2706363050197922 1105 42-43 -0.28042102919254575 1105 44-45 -0.22363385688480975 1105 46-47 -0.1984807660496699 1105 48-49 -0.22120843533768664 1105 50-51 -0.3563113038378489 1105 52-53 -0.3661624834867965 1105 54-55 -0.23145970837921936 1105 56-57 -0.29024913263410923 1105 58-59 -0.2570158886280822 1105 60-61 -0.23115729888433378 1105 62-63 -0.3138361559026137 1105 64-65 -0.2504188019606417 1105 66-67 -0.43515274101970647 1105 68-69 -0.4756449652236654 1105 70-71 -0.6266834121922145 1105 72-73 -0.3181308859935683 1105 74-75 -0.3658547241034604 1105 76-77 -0.367433426501087 1105 78-79 -0.32666094090553344 1105 80-81 -0.25478820827611415 1105 82-83 -0.2423654020927728 1105 84-85 -0.21830509045200586 1105 86-87 -0.24080572397394917 1105 88-89 -0.26205530871882843 1105 90-91 -0.12460544796496009 1105 92-93 0.013200854472955825 1105 94-95 -0.004766290958329478 1105 96-97 0.07669000888356692 1105 98-99 0.19682374521015333 1105 100-101 0.5699652769353101 1106 1 -0.2808025168547523 1106 2 -0.18718788011829446 1106 3 -0.24804652904786195 1106 4 -0.09706775656137268 1106 5 -0.3123302664649259 1106 6 -0.28068484142987415 1106 7 -0.7021027960289317 1106 8 -0.5195724103925272 1106 9 -0.5199624162939855 1106 10-11 -1.008154201732701 1106 12-13 -0.520226860195379 1106 14-15 -0.7285361073001795 1106 16-17 -0.4964722721311361 1106 18-19 -0.6885409197999834 1106 20-21 -0.6359540382434972 1106 22-23 -0.5746237553914071 1106 24-25 -0.6115170695276646 1106 26-27 -0.8324312647170657 1106 28-29 -0.4382025038169033 1106 30-31 -0.615558107745251 1106 32-33 -0.5670253413088631 1106 34-35 -0.3731514561193876 1106 36-37 -0.6908716146753164 1106 38-39 -0.48698402871520585 1106 40-41 -0.6099254214760848 1106 42-43 -0.5708056445771632 1106 44-45 -0.4254259502403741 1106 46-47 -0.5114124164426173 1106 48-49 -0.4580475637015837 1106 50-51 -0.5745507656144611 1106 52-53 -0.45032835631366197 1106 54-55 -0.5079273197152432 1106 56-57 -0.5257486563302223 1106 58-59 -0.5770176747676459 1106 60-61 -0.7516085968124102 1106 62-63 -0.7953377038902261 1106 64-65 -0.4622668610223215 1106 66-67 -0.39411023089824937 1106 68-69 -0.6142136720586322 1106 70-71 -0.5112690278149827 1106 72-73 -0.7051754204065261 1106 74-75 -0.37634829400105474 1106 76-77 -0.16366169513647577 1106 78-79 -0.22959318667833628 1106 80-81 -0.5136534142775453 1106 82-83 -0.4708275359341627 1106 84-85 -0.5806324302948269 1106 86-87 -0.37738288520281316 1106 88-89 -0.12305792839018181 1106 90-91 9.393245999369526E-4 1106 92-93 -0.016148395348427158 1106 94-95 -0.09594871534508798 1106 96-97 0.06261820607337398 1106 98-99 -0.1029648949422608 1106 100-101 0.38941931361070914 1107 1 -0.07586940648685925 1107 2 -0.10684551081711291 1107 3 -0.11172369856027231 1107 4 -0.13397147069620985 1107 5 -0.10259166410618548 1107 6 -0.039772150002306716 1107 7 -0.10729994441829405 1107 8 -0.10976339789473144 1107 9 -0.12192069828658703 1107 10-11 -0.13495846154611257 1107 12-13 -0.04250286794049174 1107 14-15 -0.030661613373048624 1107 16-17 -0.14130372786075895 1107 18-19 -0.14592958376758958 1107 20-21 -0.03398695498350435 1107 22-23 -0.08501365153644969 1107 24-25 -0.04831736877075343 1107 26-27 -0.013290708476972668 1107 28-29 0.04061944654394978 1107 30-31 0.0705226878893157 1107 32-33 -0.061289115292318286 1107 34-35 -0.04812065168354707 1107 36-37 -0.1291681293734399 1107 38-39 -0.025581986821173075 1107 40-41 0.07725773289814697 1107 42-43 -0.08391073170970742 1107 44-45 -0.08313586046744348 1107 46-47 -0.1384917158617398 1107 48-49 -0.08508065646694263 1107 50-51 -0.11313552181935194 1107 52-53 -0.2710421390983839 1107 54-55 -0.1270216693015911 1107 56-57 -0.11767569382362808 1107 58-59 -0.19890422643108252 1107 60-61 0.0361158355058393 1107 62-63 0.02258387681740004 1107 64-65 0.01928568254395202 1107 66-67 -0.07138535851662908 1107 68-69 -0.07614378999561211 1107 70-71 0.08183737950766812 1107 72-73 0.09953325764311671 1107 74-75 0.08138361939447236 1107 76-77 0.09030107550249511 1107 78-79 0.08187706503791148 1107 80-81 0.22771474273706716 1107 82-83 0.1705648245657514 1107 84-85 0.2371197260156812 1107 86-87 0.35754714472149374 1107 88-89 0.24080186742041576 1107 90-91 0.4472581945172074 1107 92-93 0.4385932874538945 1107 94-95 0.6981016279043395 1107 96-97 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92-93 0.952496599389967 2315 94-95 1.0427748664844998 2315 96-97 1.2120539445380203 2315 98-99 1.327467904282237 2315 100-101 1.7052484437096673 2316 1 -0.23432233037456385 2316 2 -0.026778114445370704 2316 3 -0.018543400597366144 2316 4 0.10788623681366971 2316 5 -0.08193644975531811 2316 6 0.1172933207588116 2316 7 0.013313717282322557 2316 8 -0.1366525232621143 2316 9 -0.31212288740182004 2316 10-11 -0.37827811290924274 2316 12-13 -0.1506856996015884 2316 14-15 0.0462031884917522 2316 16-17 0.03751987607153495 2316 18-19 -0.031185994024006902 2316 20-21 0.040924872319621386 2316 22-23 0.17434937673962025 2316 24-25 -0.025002531434175523 2316 26-27 0.23138421804578257 2316 28-29 0.31750477136404953 2316 30-31 0.23721823871530745 2316 32-33 0.2282292286825438 2316 34-35 0.21023953885055846 2316 36-37 0.06425413676622327 2316 38-39 0.0743323741801376 2316 40-41 0.06981448773750287 2316 42-43 0.21674802981861774 2316 44-45 0.22173390744580246 2316 46-47 0.4242408776317248 2316 48-49 0.3973076074430537 2316 50-51 0.23892488154539393 2316 52-53 0.3266658775226787 2316 54-55 0.2893576759908001 2316 56-57 0.43842874521560304 2316 58-59 0.5732141986221251 2316 60-61 0.550646341231996 2316 62-63 0.4104517697939869 2316 64-65 0.5200052528287458 2316 66-67 0.3079214216597208 2316 68-69 0.6467248630904265 2316 70-71 0.6006939215163882 2316 72-73 0.3876745366539609 2316 74-75 0.44691265557568727 2316 76-77 0.5758855996739811 2316 78-79 0.6945265532308795 2316 80-81 0.4550424512604252 2316 82-83 0.33791863018042534 2316 84-85 0.22408232863045896 2316 86-87 0.4563246353468102 2316 88-89 0.6849153264251804 2316 90-91 0.5215895515659561 2316 92-93 0.7736148238884724 2316 94-95 0.7853186138169761 2316 96-97 0.8627752420198824 2316 98-99 0.8641900553705852 2316 100-101 0.6014755030353207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6714.0 3 1430.0 4 1047.0 5 1195.0 6 1369.0 7 1565.0 8 1948.0 9 2441.0 10 3075.0 11 3874.0 12 4962.0 13 6227.0 14 6953.0 15 8290.0 16 10277.0 17 12576.0 18 11535.0 19 9294.0 20 9789.0 21 10715.0 22 11940.0 23 13440.0 24 15442.0 25 17799.0 26 20711.0 27 25162.0 28 29949.0 29 36991.0 30 45630.0 31 57073.0 32 73204.0 33 96439.0 34 132497.0 35 188681.0 36 282431.0 37 402931.0 38 326041.0 39 36468.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.666385923320675 19.177421996241158 14.578118135760205 23.578073944677964 2 14.977156608471025 20.140334828505644 40.55130797257753 24.331200590445796 3 18.711554412654326 24.072004856175305 29.947384504726248 27.26905622644412 4 12.615279227286146 16.543660578038526 37.683937414502104 33.15712278017322 5 13.800858411927539 37.30406888085246 34.733095012624624 14.161977694595373 6 32.241118388731685 36.36348985445163 17.649110786133786 13.746280970682898 7 28.691504050839278 32.24914117216483 20.980820898005355 18.078533878990534 8 25.941298840094856 35.60275665903238 20.106010152938644 18.349934347934116 9 25.832616475345716 17.077246537640892 20.335775415106255 36.75436157190713 10-11 24.536121542549875 26.876073467826117 28.087974457183968 20.49983053244004 12-13 25.400773800472802 23.830897174471602 28.406768999634803 22.361560025420786 14-15 22.276022574143692 25.659103401585075 26.11354903430953 25.951324989961705 16-17 22.335921656818144 28.98685772122524 26.657054676059282 22.020165945897332 18-19 22.193448855340367 27.83461990480532 28.529165780854715 21.4427654589996 20-21 22.70546378785201 27.106588563052902 28.558712849673068 21.62923479942202 22-23 22.478932359796598 26.875414342601527 28.391073406890865 22.254579890711007 24-25 22.360503310707568 27.115760423271624 28.4624439986279 22.06129226739291 26-27 22.23874212424283 27.409194248059453 28.22829378432406 22.123769843373655 28-29 22.099463596672937 27.48746061705876 28.441384200247537 21.971691586020764 30-31 22.291195405507473 27.200550815111235 28.358452966414365 22.149800812966923 32-33 22.08991821080549 27.315428496573833 28.244225009174905 22.350428283445773 34-35 22.53610969518055 27.295594574298494 28.015384067518962 22.152911663001998 36-37 22.479382614087545 27.108191704396557 28.12995229167825 22.282473389837644 38-39 22.276787732973247 27.317232392942365 28.27846549898726 22.127514375097128 40-41 22.456361467637194 27.119999792104654 28.270362699417113 22.153276040841043 42-43 22.337805440594607 27.17724487873334 27.987759636687304 22.497190043984745 44-45 22.41553681296131 27.42530073476988 27.81468796387726 22.34447448839155 46-47 22.30028917698281 27.475088540143616 27.67505604834779 22.549566234525784 48-49 22.508072519847925 27.534610067787852 27.6068221435809 22.350495268783327 50-51 22.489680055732897 27.562101629355436 27.537406548615518 22.410811766296153 52-53 22.67603539219275 27.53050226297048 27.409527979300474 22.38393436553629 54-55 22.46358690792333 27.424974681359448 27.507767155848452 22.60367125486877 56-57 22.39897296763497 27.719530766791927 27.503105525496498 22.37839074007661 58-59 22.62945022919892 27.390193165242 27.54445617158557 22.43590043397351 60-61 22.531185375553534 27.311448941312072 27.89208972741037 22.26527595572403 62-63 22.42150380609261 27.4387900536192 27.79974197303579 22.3399641672524 64-65 22.35814627104402 27.33736272701715 27.697482642272046 22.60700835966678 66-67 22.261578484042527 27.538322986724538 27.541077232160028 22.65902129707291 68-69 22.237899354370658 27.383913651395492 27.75239554807557 22.625791446158274 70-71 22.27536240545394 27.376542832948036 27.6612236233088 22.686871138289227 72-73 22.317502107353963 27.278774606150876 27.681172676220005 22.722550610275157 74-75 22.13101484705355 27.49174781134781 27.552526743119753 22.82471059847889 76-77 22.310354308833475 27.414325684284822 27.548217384818575 22.72710262206313 78-79 22.287900168136236 27.354595940302538 27.58528301200342 22.772220879557803 80-81 22.228406856420598 27.597033142623122 27.440728206895244 22.733831794061036 82-83 22.27721756040876 27.459517665361275 27.537082226490078 22.726182547739885 84-85 22.311281017484177 27.274754092226523 27.451892919487975 22.962071970801325 86-87 22.406241765506575 27.18356027402315 27.568889089851673 22.841308870618597 88-89 22.31916453884417 26.965258241338468 27.636009564936742 23.079567654880616 90-91 22.170182630898474 27.09249790471488 27.836405335343073 22.900914129043574 92-93 22.210514307749996 26.942890872045027 27.850096886962266 22.99649793324271 94-95 22.006029568897777 27.039721772969738 27.7998621753429 23.154386482789587 96-97 22.108269778656386 27.02744985120838 27.733609078380738 23.130671291754492 98-99 22.005882747354242 27.018640481389404 27.60621352844775 23.369263242808604 100-101 22.118806880098198 26.795882001711764 27.963299491304262 23.122011626885783 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2510.0 1 1427.0 2 296.0 3 225.0 4 188.0 5 164.5 6 213.0 7 382.0 8 582.5 9 714.0 10 747.5 11 773.0 12 851.5 13 928.0 14 1021.5 15 1141.5 16 1219.5 17 1318.5 18 1442.5 19 1586.5 20 1804.0 21 2092.0 22 2491.5 23 3071.0 24 3904.0 25 5124.0 26 7069.5 27 9666.5 28 12398.5 29 15774.5 30 20293.0 31 24717.5 32 29082.5 33 33984.0 34 39461.5 35 43722.0 36 48106.0 37 53361.5 38 57697.0 39 60457.0 40 62860.5 41 67237.5 42 71428.5 43 74911.0 44 77495.5 45 79366.5 46 78713.5 47 77866.0 48 80804.0 49 81207.0 50 78826.5 51 76106.5 52 71296.0 53 66795.5 54 62619.5 55 56838.5 56 49918.5 57 42742.5 58 37189.0 59 32333.0 60 26519.5 61 21217.5 62 16637.5 63 12213.5 64 8815.5 65 6584.5 66 4917.0 67 3884.0 68 2805.0 69 1817.0 70 1367.5 71 1020.0 72 826.0 73 663.0 74 464.5 75 342.0 76 228.0 77 141.0 78 98.0 79 66.5 80 43.5 81 27.0 82 20.0 83 15.0 84 14.5 85 10.5 86 7.5 87 6.0 88 6.0 89 5.0 90 1.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.5 96 0.5 97 1.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24070253398931385 2 0.21451102792066412 3 0.20527917033607074 4 0.21171035202421445 5 0.21202153823493106 6 0.23613846956546994 7 0.23619033393392272 8 0.2244689866635963 9 0.21788221187009427 10-11 0.22996660971959013 12-13 0.23253389595800233 14-15 0.21904916016028164 16-17 0.23167813387853156 18-19 0.22089034524035503 20-21 0.21606695897424727 22-23 0.21391458768345722 24-25 0.20984323475991465 26-27 0.21596323023734174 28-29 0.2264916970332544 30-31 0.22879966142940275 32-33 0.22716593382314043 34-35 0.23637185922350742 36-37 0.21383679113077808 38-39 0.21362933365696699 40-41 0.21062120028670622 42-43 0.21490001068405992 44-45 0.23040745685143868 46-47 0.23564575806516863 48-49 0.2388354167250141 50-51 0.24096185583157773 52-53 0.2577659112102758 54-55 0.2397171109887112 56-57 0.21347374055160867 58-59 0.1968252782782689 60-61 0.19534714377736495 62-63 0.2016745967286031 64-65 0.1943357885925359 66-67 0.19729205759434387 68-69 0.20017053004347274 70-71 0.19944442888513392 72-73 0.20185612201818778 74-75 0.20328239215063904 76-77 0.21591136586888898 78-79 0.21113984397123395 80-81 0.20654984736316362 82-83 0.20240069788694193 84-85 0.20273781628188495 86-87 0.20927272670693414 88-89 0.2071981519688233 90-91 0.21490001068405992 92-93 0.20520137378339157 94-95 0.20341205307177093 96-97 0.21298102905130734 98-99 0.2165337382903222 100-101 0.19568426217230794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1928106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.196439062436774 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64091670392845 43.263910828831 2 13.611150778096684 15.570187120980917 3 4.669019150092157 8.011538078987893 4 2.122247328998978 4.8553996011403715 5 1.1417068600105016 3.2650783422878344 6 0.6751803232596312 2.3170746129285513 7 0.42845317056602283 1.715419696297115 8 0.30167362686646626 1.3803725772649702 9 0.2095375018489708 1.0786319060219902 >10 1.0874890188617763 11.071809659461461 >50 0.07499549151499396 2.9585582543844193 >100 0.03627045820690666 3.6234926814905033 >500 9.970310154531557E-4 0.3632077507030848 >1k 3.6255673289205557E-4 0.5253188892198615 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3327 0.17255275384237173 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2750 0.14262701324512242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2398 0.12437075554974673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.186436845277179E-5 0.0 18-19 0.0 0.0 0.0 5.186436845277179E-5 0.0 20-21 0.0 0.0 0.0 5.186436845277179E-5 0.0 22-23 0.0 0.0 0.0 1.5559310535831537E-4 0.0 24-25 0.0 0.0 0.0 2.5932184226385894E-4 0.0 26-27 0.0 0.0 0.0 6.223724214332615E-4 0.0 28-29 0.0 0.0 0.0 0.001478134500903996 0.0 30-31 0.0 0.0 0.0 0.0027747437122232904 0.0 32-33 0.0 0.0 0.0 0.004953047187239706 0.0 34-35 0.0 0.0 0.0 0.007572197794104681 0.0 36-37 0.0 0.0 0.0 0.012499312797118002 0.0 38-39 0.0 0.0 0.0 0.02027896806503377 0.0 40-41 0.0 0.0 0.0 0.02816235206985508 0.0 42-43 0.0 0.0 0.0 0.040143021182445365 0.0 44-45 0.0 0.0 0.0 0.05095674200484828 0.0 46-47 0.0 0.0 0.0 0.07084672730648627 0.0 48-49 0.0 0.0 0.0 0.09939806213973713 0.0 50-51 0.0 0.0 0.0 0.11485364393866312 0.0 52-53 0.0 0.0 0.0 0.141589725876067 0.0 54-55 0.0 0.0 0.0 0.18025461255760833 0.0 56-57 0.0 0.0 0.0 0.24596676738727022 0.0 58-59 0.0 0.0 0.0 0.32804213046378156 0.0 60-61 0.0 0.0 0.0 0.4225649419689581 0.0 62-63 0.0 0.0 0.0 0.5322580812465705 0.0 64-65 0.0 0.0 0.0 0.6637083230901206 0.0 66-67 0.0 0.0 0.0 0.8321637918247233 0.0 68-69 0.0 0.0 0.0 1.0378060127399635 0.0 70-71 0.0 0.0 0.0 1.1693599833204191 0.0 72-73 0.0 0.0 0.0 1.333588506026121 0.0 74-75 0.0 0.0 0.0 1.5365078475975906 0.0 76-77 0.0 0.0 0.0 1.7670449653701612 0.0 78-79 0.0 0.0 0.0 2.0068398729115513 0.0 80-81 0.0 0.0 0.0 2.33576369763903 0.0 82-83 0.0 0.0 0.0 2.6945354664110788 0.0 84-85 0.0 0.0 0.0 3.1534054662969773 0.0 86-87 0.0 0.0 0.0 3.7531909552690568 0.0 88-89 0.0 0.0 0.0 4.400328612638517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4985 0.0 36.69942 1 GGTATCA 3130 0.0 35.828724 1 ATCAACG 5970 0.0 30.398424 3 TCAACGC 6045 0.0 30.101427 4 CAACGCA 6090 0.0 29.957018 5 TATCAAC 6260 0.0 28.990192 2 AACGCAG 6370 0.0 28.7864 6 ACGCAGA 7265 0.0 25.04394 7 CGCAGAG 7410 0.0 24.551962 8 GTACATG 6025 0.0 23.50298 1 TACATGG 6060 0.0 22.65621 2 GTATAGG 520 0.0 21.931664 1 GTGTAGC 1000 0.0 21.383371 1 GCAGAGT 8290 0.0 21.257574 9 ACATGGG 6065 0.0 21.227583 3 GAGTACT 3965 0.0 20.365204 12-13 CATGGGG 3380 0.0 19.256996 4 GTACTTT 4340 0.0 18.76946 14-15 AGAGTAC 7145 0.0 18.68044 10-11 AGTACTT 4330 0.0 18.429111 12-13 >>END_MODULE