FastQCFastQC Report
Thu 26 May 2016
SRR937240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.23702016382916705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34330.17805037689836556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.12442261991819951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.040.1056481
GTATCAA55100.037.8749541
ATCAACG66900.030.9555873
TCAACGC67700.030.5897884
CAACGCA69000.030.013465
TATCAAC70100.029.6780092
AACGCAG71600.028.9899256
ACGCAGA80350.025.4789377
CGCAGAG81050.025.0244648
GTACATG56300.022.5445791
GCAGAGT89600.022.2654349
TACATGG58150.020.99242
GAGTACT50250.020.3232112-13
ACATGGG56850.020.3027323
AGAGTAC80800.019.57594910-11
AGTACTT53100.019.50077412-13
GTACTTT52950.019.1069914-15
CAGAGTA87150.018.42210410-11
CATGGGG31750.017.9521394
TACTTTT62400.016.7081614-15
Produced by FastQC (version 0.11.5)