FastQCFastQC Report
Thu 26 May 2016
SRR937239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959546
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73160.37335178658730134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66970.3417628369020171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.23724883212744174No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43780.2234190980972123No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35590.18162370263316094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31680.1616701011356712No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30790.15712823276411986No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28780.14687075475645892No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23760.12125257585175342No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22120.11288329031316437No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19930.10170723218541437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95200.027.4146771
TACATGG100300.025.7251322
ACATGGG97950.024.6443843
GAGTACT60100.022.32929212-13
CATGGGA59850.021.5144634
GTACCTG15700.021.4984131
GTAAGCG2350.021.22034594-95
GTACTTT65100.020.61218514-15
AGTACTT65850.020.01880612-13
GTGTAGC10450.020.0163121
TACCTGG16150.019.7133752
GTATAGG6650.019.3014431
AGAGTAC115900.019.20247310-11
GTATCAA173100.019.1418271
AGTACAT70150.019.1020582
CATGGGG38500.018.3886954
GAGTACA81700.018.2707021
ATGGGAG27200.017.4680885
TACTTTT75100.017.32994514-15
TAGCCCT12250.017.0664024
Produced by FastQC (version 0.11.5)