##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937239_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1959546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.570456115855407 31.0 31.0 34.0 30.0 34.0 2 31.67539419845209 33.0 31.0 34.0 30.0 34.0 3 31.720601608739983 33.0 31.0 34.0 30.0 34.0 4 35.35519298858001 37.0 35.0 37.0 33.0 37.0 5 35.364913607539705 37.0 35.0 37.0 33.0 37.0 6 35.406927420943425 37.0 35.0 37.0 33.0 37.0 7 35.340630942065154 37.0 35.0 37.0 33.0 37.0 8 35.31927701620682 37.0 35.0 37.0 33.0 37.0 9 36.883693978094925 39.0 37.0 39.0 33.0 39.0 10-11 36.76579779193752 39.0 37.0 39.0 33.0 39.0 12-13 36.617674961445154 39.0 37.0 39.0 32.0 39.0 14-15 37.6611704956148 40.0 37.0 41.0 32.0 41.0 16-17 37.58499647367299 40.0 37.0 41.0 32.0 41.0 18-19 37.50552602490577 40.0 37.0 41.0 32.0 41.0 20-21 37.35388962545406 39.5 37.0 41.0 32.0 41.0 22-23 37.447560812555565 40.0 37.0 41.0 32.0 41.0 24-25 37.408752843770955 40.0 37.0 41.0 32.0 41.0 26-27 37.32242927698559 40.0 37.0 41.0 32.0 41.0 28-29 37.18586677730454 39.0 37.0 41.0 31.0 41.0 30-31 36.948462041717825 39.0 36.0 41.0 31.0 41.0 32-33 36.714152410813526 39.0 36.0 40.5 30.0 41.0 34-35 36.69071356324373 39.0 36.0 41.0 30.0 41.0 36-37 36.53670237902044 39.0 35.5 41.0 30.0 41.0 38-39 36.384052479502905 39.0 35.0 41.0 30.0 41.0 40-41 36.159550987830855 39.0 35.0 40.0 29.5 41.0 42-43 35.88651146745215 39.0 35.0 40.0 29.0 41.0 44-45 35.63102116510662 38.0 35.0 40.0 27.5 41.0 46-47 35.24890025546733 38.0 34.5 40.0 26.5 41.0 48-49 34.90049378784678 38.0 34.0 40.0 24.5 41.0 50-51 33.93949899619606 37.5 33.0 39.5 23.5 40.5 52-53 34.05779731631715 37.5 33.5 39.5 24.0 40.0 54-55 34.34412027071576 38.0 34.0 40.0 23.5 41.0 56-57 34.051253708767234 38.0 33.0 40.0 23.0 41.0 58-59 33.75273941004702 38.0 33.0 40.0 22.0 41.0 60-61 33.37149446861671 37.0 32.5 40.0 20.5 41.0 62-63 32.963362687071395 37.0 32.0 39.5 18.5 41.0 64-65 32.509197538613535 36.0 31.0 39.0 17.5 40.5 66-67 32.01741474811002 36.0 31.0 39.0 13.5 40.0 68-69 31.48636674005101 35.0 30.5 38.5 8.0 40.0 70-71 30.966358023746317 35.0 30.0 38.0 7.5 40.0 72-73 30.42132922625955 34.5 29.0 37.0 4.0 39.0 74-75 29.82364307854983 34.0 29.0 36.5 2.0 39.0 76-77 29.24816819814386 34.0 28.0 36.0 2.0 39.0 78-79 28.63370979808588 34.0 27.5 35.5 2.0 37.5 80-81 27.964463707409777 33.0 26.0 35.0 2.0 37.0 82-83 27.338705240907842 33.0 25.5 35.0 2.0 36.5 84-85 26.668038923301623 32.0 24.5 35.0 2.0 36.0 86-87 25.99857313888013 32.0 23.0 35.0 2.0 36.0 88-89 25.336065854029457 31.5 20.0 34.0 2.0 35.0 90-91 24.66249223034315 31.0 18.0 34.0 2.0 35.0 92-93 23.88826391419237 31.0 9.0 34.0 2.0 35.0 94-95 23.005668404824384 30.5 2.0 34.0 2.0 35.0 96-97 22.0229310768923 30.0 2.0 34.0 2.0 35.0 98-99 20.740306172960473 29.0 2.0 34.0 2.0 35.0 100-101 17.696995630620563 23.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19616458527178793 1101 2 0.19045745790378632 1101 3 0.22220585101527135 1101 4 0.10312161731165048 1101 5 0.05806171507800428 1101 6 0.08047569491015594 1101 7 0.2211161164451383 1101 8 0.06146284721850748 1101 9 0.25700436868051924 1101 10-11 0.22924201864202587 1101 12-13 0.30898487225083926 1101 14-15 0.4085476404804993 1101 16-17 0.48834641855122385 1101 18-19 0.4992817747290488 1101 20-21 0.5988950715420458 1101 22-23 0.6932734088028312 1101 24-25 0.5895592911764496 1101 26-27 0.556407430364807 1101 28-29 0.5599628040746296 1101 30-31 0.8351905171036478 1101 32-33 0.6712523325433324 1101 34-35 0.6915639002473952 1101 36-37 0.7055363894196702 1101 38-39 0.6929067970172511 1101 40-41 0.7299079511796549 1101 42-43 0.9222394913739507 1101 44-45 0.9544709752696079 1101 46-47 0.908213684285208 1101 48-49 0.9703537754555427 1101 50-51 0.9919367880826968 1101 52-53 0.8835780285062356 1101 54-55 0.9627851867735515 1101 56-57 1.0948261342572536 1101 58-59 1.1121567235443308 1101 60-61 1.1562772365104408 1101 62-63 1.2075987866606397 1101 64-65 1.2231327744253733 1101 66-67 1.2613183719698426 1101 68-69 1.3332616804056308 1101 70-71 1.2423134601682158 1101 72-73 1.3065791924984254 1101 74-75 1.449331356457499 1101 76-77 1.4788094918280876 1101 78-79 1.6739645871060276 1101 80-81 1.8337211062878822 1101 82-83 1.5960308523865585 1101 84-85 1.7862188547253481 1101 86-87 1.748318723985669 1101 88-89 2.0010066491515417 1101 90-91 2.090823443177758 1101 92-93 2.339135572716412 1101 94-95 2.5873612468947975 1101 96-97 2.401738808067389 1101 98-99 2.462194022746072 1101 100-101 2.3927080809222296 1102 1 0.26476632132755284 1102 2 0.3288794054356785 1102 3 0.2658004621899863 1102 4 0.20363195201199602 1102 5 0.1887030509811254 1102 6 0.21106751892800446 1102 7 0.19734558108882538 1102 8 0.17132247498573605 1102 9 0.3277967037576417 1102 10-11 0.24792611349231208 1102 12-13 0.36520984459664163 1102 14-15 0.347288154727849 1102 16-17 0.5063319127764458 1102 18-19 0.475270321784663 1102 20-21 0.48509804128907774 1102 22-23 0.47124702017558917 1102 24-25 0.4881764604491323 1102 26-27 0.6747148726049659 1102 28-29 0.5728304600129093 1102 30-31 0.6776123004009946 1102 32-33 0.7605693583523063 1102 34-35 0.6824386182711706 1102 36-37 0.8361523655766803 1102 38-39 0.798376773493068 1102 40-41 0.875102158530602 1102 42-43 0.9396151440855149 1102 44-45 1.036793541579307 1102 46-47 1.1278936925935383 1102 48-49 1.0461836429569047 1102 50-51 0.9447453195843352 1102 52-53 0.8826397179000622 1102 54-55 1.1800149605646126 1102 56-57 1.1401977390702953 1102 58-59 0.9768866301721602 1102 60-61 1.0793478428740428 1102 62-63 1.1459836606589349 1102 64-65 0.9951498644790462 1102 66-67 1.2171072774431977 1102 68-69 1.377725164786444 1102 70-71 1.2858639936889666 1102 72-73 1.4239767028959207 1102 74-75 1.484600121462016 1102 76-77 1.6799931881680727 1102 78-79 1.622266329441345 1102 80-81 1.735834901506724 1102 82-83 1.889260162456555 1102 84-85 2.1570785742707095 1102 86-87 2.120160227987679 1102 88-89 2.0762490158679654 1102 90-91 1.9810266109723287 1102 92-93 2.3258084223306987 1102 94-95 2.384167488773638 1102 96-97 2.3172522100894035 1102 98-99 2.380657004762387 1102 100-101 2.1407046609793277 1103 1 0.34666574800787586 1103 2 0.39931912061036456 1103 3 0.35225977884735826 1103 4 0.3000936863556518 1103 5 0.22351105145737193 1103 6 0.2583773265943847 1103 7 0.2641198894665351 1103 8 0.36315251771439705 1103 9 0.3778434173444296 1103 10-11 0.42841687343550205 1103 12-13 0.4568823732413634 1103 14-15 0.5338563887598795 1103 16-17 0.4601401331528052 1103 18-19 0.560643350125055 1103 20-21 0.582944933711758 1103 22-23 0.34710080361164586 1103 24-25 0.6007637978475842 1103 26-27 0.5908255625446017 1103 28-29 0.41005836400039186 1103 30-31 0.6899356665492604 1103 32-33 0.5749489120158344 1103 34-35 0.680061935458248 1103 36-37 0.5855734572774551 1103 38-39 0.6991061493533834 1103 40-41 0.802383561420406 1103 42-43 0.5848300083609814 1103 44-45 0.6607468899533231 1103 46-47 0.5305362601520827 1103 48-49 0.5248715561048698 1103 50-51 0.5397105790117962 1103 52-53 0.5291578851723671 1103 54-55 0.6730329802244128 1103 56-57 0.625296924953922 1103 58-59 0.7426189036112589 1103 60-61 0.7208976040819621 1103 62-63 0.6016338481814856 1103 64-65 0.4989598464861089 1103 66-67 0.812236247035024 1103 68-69 0.7471219642071709 1103 70-71 1.0033739043803642 1103 72-73 1.1266454823045216 1103 74-75 0.8731232188704752 1103 76-77 0.7432217591453885 1103 78-79 0.7798069897005355 1103 80-81 0.6601841004507172 1103 82-83 0.9546971501409338 1103 84-85 1.116604653087279 1103 86-87 1.1991148459507244 1103 88-89 1.324710930019446 1103 90-91 1.0686897592372446 1103 92-93 1.46029556303397 1103 94-95 1.4549466215896594 1103 96-97 1.6181674379897473 1103 98-99 1.8106153270095966 1103 100-101 1.6252021588562577 1104 1 0.3199539592966474 1104 2 0.32998315819506274 1104 3 0.266904214949367 1104 4 0.29634718380007286 1104 5 0.35426596488841255 1104 6 0.30930151451299537 1104 7 0.3540784729210529 1104 8 0.36834234253540643 1104 9 0.4630064167819228 1104 10-11 0.44163472276383686 1104 12-13 0.44164472318383474 1104 14-15 0.7766479781274072 1104 16-17 0.5289588443437765 1104 18-19 0.5279745160451554 1104 20-21 0.41914881391601 1104 22-23 0.5173286978797833 1104 24-25 0.5419844074690019 1104 26-27 0.6018671904857626 1104 28-29 0.4856339920217465 1104 30-31 0.5802061193855437 1104 32-33 0.6057680338489959 1104 34-35 0.6824386182711706 1104 36-37 0.6929404450468795 1104 38-39 0.8833657359654765 1104 40-41 0.9686452048882117 1104 42-43 0.9089860050126646 1104 44-45 0.820733938930303 1104 46-47 0.9935117941387972 1104 48-49 0.9156648791599977 1104 50-51 0.765385496184777 1104 52-53 0.7620547289375992 1104 54-55 1.0155557994167097 1104 56-57 1.1360586662226098 1104 58-59 1.1154076014745868 1104 60-61 1.0854184830506455 1104 62-63 1.0676172147428247 1104 64-65 1.1957569284967065 1104 66-67 1.2814008756771926 1104 68-69 1.2935640168835683 1104 70-71 1.1920450091415056 1104 72-73 1.3748597051034253 1104 74-75 1.4365868764289047 1104 76-77 1.5218806053866167 1104 78-79 1.7039440336356044 1104 80-81 1.7733624953257063 1104 82-83 1.7496354383947441 1104 84-85 1.7061955720632014 1104 86-87 1.7459880425572152 1104 88-89 1.9126176692895953 1104 90-91 1.7925608273078701 1104 92-93 1.9789541176949363 1104 94-95 2.0287591002526675 1104 96-97 2.2278482365794723 1104 98-99 2.1091338259544408 1104 100-101 1.8774596278667453 1105 1 0.39456134573117296 1105 2 0.2505666956639807 1105 3 0.301344451091925 1105 4 0.30080326901695287 1105 5 0.34078436920903954 1105 6 0.4047946579045032 1105 7 0.41354894336555503 1105 8 0.4355776858476048 1105 9 0.41023968476716277 1105 10-11 0.5049162902780466 1105 12-13 0.47833523758080077 1105 14-15 0.5700232380065202 1105 16-17 0.5774859798613932 1105 18-19 0.6279765543126956 1105 20-21 0.679592155637124 1105 22-23 0.643809066892679 1105 24-25 0.6824372549287645 1105 26-27 0.7576104416583789 1105 28-29 0.5651553018001181 1105 30-31 0.5612397310350445 1105 32-33 0.6765524333197916 1105 34-35 0.7065528674907569 1105 36-37 0.6962583431359732 1105 38-39 0.8025030299614784 1105 40-41 0.6586211595077387 1105 42-43 0.6255261446772309 1105 44-45 0.6049993781893122 1105 46-47 0.57948422070249 1105 48-49 0.4930892439925927 1105 50-51 0.40542837392207787 1105 52-53 0.45504176061474766 1105 54-55 0.714671531012705 1105 56-57 0.8038093206300232 1105 58-59 0.792399946235804 1105 60-61 0.7738192583335035 1105 62-63 0.8980362887269919 1105 64-65 0.8495910024005724 1105 66-67 1.0569244633739672 1105 68-69 0.8615136803223642 1105 70-71 0.9212430990995202 1105 72-73 1.0704535574414429 1105 74-75 1.1224107874072615 1105 76-77 1.1705183323600608 1105 78-79 1.056649710272488 1105 80-81 1.122397591822235 1105 82-83 1.3517699368759253 1105 84-85 1.3432596453123615 1105 86-87 1.1787485300614016 1105 88-89 1.371281757856643 1105 90-91 1.795285311595297 1105 92-93 2.196085046119631 1105 94-95 2.071057012201983 1105 96-97 2.1880888942110523 1105 98-99 1.9916411359230501 1105 100-101 1.5631110581746697 1106 1 0.19026487661847113 1106 2 -0.24829991519018435 1106 3 0.15602932600904396 1106 4 0.16120839964533218 1106 5 0.3329698828996115 1106 6 0.20999052004047059 1106 7 0.2578304856832716 1106 8 0.36217961926501374 1106 9 0.3340508879387585 1106 10-11 0.015043925100258093 1106 12-13 0.08109243616332407 1106 14-15 0.12296005022100331 1106 16-17 0.24295622121996985 1106 18-19 0.34142972259748205 1106 20-21 0.25737126178329106 1106 22-23 0.3603450662718899 1106 24-25 0.42005861399893973 1106 26-27 0.3897341851673417 1106 28-29 0.4728036439227594 1106 30-31 0.5516958247445274 1106 32-33 0.502266014242764 1106 34-35 0.5569144290379242 1106 36-37 0.5313013286463857 1106 38-39 0.5932741257308152 1106 40-41 0.62312112764792 1106 42-43 0.7175175658708852 1106 44-45 0.6582159889488466 1106 46-47 0.6656210071767745 1106 48-49 0.7693532425945477 1106 50-51 0.5477925233691963 1106 52-53 0.6111072063461336 1106 54-55 0.8118726623199208 1106 56-57 0.6439566580645462 1106 58-59 0.7696430504792175 1106 60-61 0.6145851808264666 1106 62-63 0.6842787172900415 1106 64-65 0.9850524554982059 1106 66-67 1.074347995094037 1106 68-69 0.9257667191490633 1106 70-71 0.8646476133509502 1106 72-73 0.99066570593466 1106 74-75 0.8954269778696329 1106 76-77 1.242317888089179 1106 78-79 1.4280665066390803 1106 80-81 1.3428118135375051 1106 82-83 1.456547394513418 1106 84-85 1.3175703850971914 1106 86-87 1.5816032322857225 1106 88-89 1.8037235001404213 1106 90-91 1.922933066992698 1106 92-93 1.9487735726127973 1106 94-95 1.9137034521992327 1106 96-97 1.7976363334707948 1106 98-99 1.7937458077070545 1106 100-101 1.3178056487571617 1107 1 0.23422715227072644 1107 2 0.2813414793816911 1107 3 0.2037398383737603 1107 4 0.1886836674403014 1107 5 -0.056771924393913764 1107 6 0.07914796568564952 1107 7 0.17214071469052783 1107 8 0.11764126964673238 1107 9 0.2032486636873756 1107 10-11 0.23335293004269886 1107 12-13 0.2886189878409624 1107 14-15 0.2970309339182151 1107 16-17 0.27796721502144806 1107 18-19 0.3905988916803622 1107 20-21 0.37531849646200754 1107 22-23 0.40632076846208776 1107 24-25 0.23594845924832697 1107 26-27 0.28846575723476775 1107 28-29 0.2952355861035585 1107 30-31 0.26127582520016546 1107 32-33 0.3973061503922679 1107 34-35 0.4203359180253443 1107 36-37 0.5937454129590876 1107 38-39 0.745223331966649 1107 40-41 0.7262802985595087 1107 42-43 0.626808236317288 1107 44-45 0.2935102478508682 1107 46-47 0.6911301054633867 1107 48-49 0.6527093475562609 1107 50-51 0.6457814365929764 1107 52-53 0.7766044026342769 1107 54-55 0.8631346866940817 1107 56-57 0.9593762419933398 1107 58-59 0.8993743719458536 1107 60-61 0.8460023929757341 1107 62-63 1.028988731531797 1107 64-65 0.9712320063501494 1107 66-67 1.242026711328343 1107 68-69 1.2196897422197317 1107 70-71 1.200456527473687 1107 72-73 1.1999717079776424 1107 74-75 1.1263787005807728 1107 76-77 1.260805854017324 1107 78-79 1.2007782042778317 1107 80-81 1.1819635082946505 1107 82-83 1.2130265333825854 1107 84-85 1.3029790460330872 1107 86-87 1.2681395044418764 1107 88-89 1.3893184492105775 1107 90-91 1.7247617139269629 1107 92-93 1.754907227191314 1107 94-95 1.3908860034877328 1107 96-97 1.5146302999641783 1107 98-99 1.8031530352310305 1107 100-101 1.7371882348957968 1108 1 0.31535681084007905 1108 2 0.2848677194988767 1108 3 0.29611723558917546 1108 4 0.31019144902129625 1108 5 0.4069073217393253 1108 6 0.4131325929850789 1108 7 0.3116440970331311 1108 8 0.2571674833276347 1108 9 0.34524546850165905 1108 10-11 0.37908235825585734 1108 12-13 0.4087097243859361 1108 14-15 0.5664814316714413 1108 16-17 0.41528870249516103 1108 18-19 0.4368475225510977 1108 20-21 0.465816063422416 1108 22-23 0.41047231687744556 1108 24-25 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-0.4894234627448313 2316 74-75 -0.4140235346528378 2316 76-77 -0.20372202182517185 2316 78-79 -0.3467807224957511 2316 80-81 -0.1592393099094096 2316 82-83 -0.15910024317647853 2316 84-85 0.12036478405534723 2316 86-87 0.32773619077455507 2316 88-89 0.5551788582912991 2316 90-91 0.5834658422996029 2316 92-93 0.5768086496197036 2316 94-95 0.5612516977923434 2316 96-97 0.635102258505075 2316 98-99 0.8763124377479379 2316 100-101 1.5931790753987904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7839.0 3 1536.0 4 1434.0 5 1801.0 6 2021.0 7 2517.0 8 3096.0 9 3913.0 10 4958.0 11 6289.0 12 8104.0 13 10269.0 14 11458.0 15 13990.0 16 17266.0 17 21397.0 18 19721.0 19 16269.0 20 17216.0 21 18632.0 22 20772.0 23 23563.0 24 26642.0 25 30517.0 26 35114.0 27 41071.0 28 48010.0 29 56213.0 30 66634.0 31 80257.0 32 98273.0 33 123247.0 34 156295.0 35 202154.0 36 261167.0 37 299853.0 38 184298.0 39 15740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26724453843618 19.672121918113906 14.72805291744036 22.332580626009552 2 14.805463764623664 20.642088013903447 40.54331704886313 24.009131172609756 3 19.370312388085665 25.304355833870524 29.658859522567045 25.66647225547677 4 12.11442633350728 16.55615925346103 38.58893288721082 32.740481525820876 5 13.17872131209605 38.23877156245252 34.26923897467171 14.31326815077972 6 30.902527555877658 37.028419979654345 17.82075471215609 14.2482977523119 7 28.19286240721523 33.09027172197994 20.919273096266004 17.79759277453883 8 26.07972716759931 34.68440200021175 19.90785564391853 19.328015188270417 9 26.075009347907375 17.383015606785328 20.863455078637514 35.67851996666979 10-11 24.551856962538476 27.11320047726898 27.5250176162384 20.809924943954144 12-13 25.88274439376556 24.44405683650885 28.026824401499535 21.646374368226063 14-15 22.52307567984198 25.84509387033634 26.819522534046442 24.81230791577524 16-17 21.59961375970811 29.46450330506002 27.09972768320522 21.83615525202665 18-19 21.41852639761082 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28.735317695221756 22.32600182318203 44-45 21.85231409495712 27.271144276698777 28.69836745224414 22.17817417609996 46-47 22.1207966093113 27.6957217974087 28.384154964334247 21.79932662894575 48-49 22.05749521521067 27.70068240148858 28.313063830266056 21.928758553034697 50-51 21.62778004556722 27.80406196896641 28.597933208315045 21.970224777151323 52-53 22.142813475760907 28.053313906895834 27.859629323723112 21.94424329362015 54-55 21.752492004462056 27.624589993724275 28.215259879917326 22.407658121896343 56-57 22.012895400395074 27.903165350859922 27.998737970966175 22.08520127777883 58-59 22.434834183803904 27.438592275204776 28.16119399932508 21.96537954166624 60-61 22.144712130656004 27.668426792846258 28.62430978493464 21.562551291563103 62-63 21.79600790544151 27.83035004333675 28.55196447153453 21.821677579687208 64-65 21.64945475369644 28.001896953497795 28.230400597208 22.118247695597766 66-67 21.825068518367015 27.879049742289126 27.770443017262537 22.525438722081322 68-69 21.64220354713103 27.7518388885622 28.287666855008396 22.31829070929837 70-71 22.03492575809175 27.743995042017065 28.059979525821294 22.16109967406989 72-73 21.971698971885104 27.415783655090102 28.42260093740189 22.18991643562291 74-75 21.582145931701085 28.275248259390956 27.939514171220548 22.20309163768741 76-77 21.82994345529496 27.738025131491433 27.992838130969023 22.439193282244585 78-79 21.883123624593484 27.693955652521236 28.03801504706876 22.384905675816526 80-81 21.986637078106693 28.003582741984022 27.78419988284239 22.22558029706689 82-83 21.93578177277406 27.698424995129407 28.160096092631825 22.20569713946471 84-85 21.844069609508075 27.704580123204526 28.00434695134375 22.447003315943654 86-87 21.867905716330913 27.779450864096532 27.981899989774007 22.370743429798548 88-89 21.894707937097213 27.607590347460537 27.716636777973285 22.78106493746897 90-91 21.85434154762489 27.5300467985535 28.02472469032775 22.590886963493855 92-93 22.173023269133214 27.416172006676376 28.07626695684901 22.334537767341395 94-95 21.899024381139483 27.236280064153224 28.10868413025876 22.756011424448534 96-97 21.634312858398296 27.224476134735642 28.460816824031436 22.680394182834622 98-99 21.742583492996513 27.42182811505568 27.832047206263006 23.003541185684803 100-101 22.08235128390579 26.381535502189337 28.64150830520447 22.8946049087004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2801.0 1 1557.5 2 309.5 3 310.5 4 268.0 5 255.0 6 410.5 7 777.0 8 1250.5 9 1525.0 10 1569.0 11 1603.0 12 1694.5 13 1848.5 14 2047.5 15 2206.0 16 2316.0 17 2436.0 18 2655.5 19 2946.0 20 3273.5 21 3742.5 22 4400.0 23 5388.0 24 6619.0 25 8125.5 26 10408.5 27 13247.5 28 15847.0 29 19163.5 30 23382.5 31 27576.0 32 32491.5 33 37786.5 34 43664.5 35 48491.0 36 53684.5 37 59632.0 38 63018.5 39 64329.0 40 64591.5 41 66704.0 42 69028.0 43 70885.5 44 72812.5 45 73546.5 46 73087.0 47 77205.5 48 89477.0 49 89185.5 50 76164.5 51 67475.5 52 62386.5 53 59383.5 54 55785.5 55 50122.0 56 44454.5 57 39550.5 58 34934.0 59 30372.5 60 25690.5 61 21296.5 62 17418.5 63 13588.5 64 10120.0 65 7790.0 66 5856.5 67 4542.5 68 3491.0 69 2354.5 70 1670.5 71 1232.5 72 962.0 73 797.5 74 578.5 75 438.5 76 331.5 77 213.5 78 124.0 79 70.5 80 50.0 81 28.5 82 12.0 83 14.5 84 13.0 85 8.5 86 7.5 87 6.0 88 3.5 89 5.5 90 4.5 91 3.5 92 4.0 93 2.5 94 1.5 95 2.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29139402698380135 2 0.2512316628443527 3 0.25138475953103423 4 0.25138475953103423 5 0.2517419851332911 6 0.22132677671256504 7 0.2219391634592911 8 0.2221432923748664 9 0.23286006044257193 10-11 0.23849912173534074 12-13 0.2314311580335445 14-15 0.2219391634592911 16-17 0.21874964915342635 18-19 0.2074970426823356 20-21 0.21341678123402053 22-23 0.21009968635592124 24-25 0.21288094283063527 26-27 0.20795633274238012 28-29 0.22229638906154794 30-31 0.2331152215870411 32-33 0.22663412851752396 34-35 0.22464387159066437 36-37 0.21436087746855648 38-39 0.20343998048527567 40-41 0.20073527235390237 42-43 0.2129830072884229 44-45 0.20951281572364214 46-47 0.21989787430353766 48-49 0.224133549301726 50-51 0.23638128423624657 52-53 0.2460518916116284 54-55 0.22418458153061985 56-57 0.2023427875640582 58-59 0.19218737401418493 60-61 0.19468795322998286 62-63 0.2006076917816678 64-65 0.19282527687535786 66-67 0.19729059690356845 68-69 0.19805608033697603 70-71 0.19963807943268494 72-73 0.2004545950949863 74-75 0.20313378711191268 76-77 0.21385055517961815 78-79 0.21510084478751712 80-81 0.2072163654234195 82-83 0.20070975623945547 84-85 0.188436505190488 86-87 0.1911667294363082 88-89 0.20193452973290751 90-91 0.20055665955277394 92-93 0.20382272220197944 94-95 0.19981669223381335 96-97 0.20706326873673797 98-99 0.21063552475930647 100-101 0.19466243711553594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1959546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.853521995621215 #Duplication Level Percentage of deduplicated Percentage of total 1 76.82856579614446 41.374888579946976 2 13.04430957011454 14.049640247037113 3 4.277343687326901 6.910500670448724 4 1.918045068778485 4.131739292002198 5 1.0096572093819185 2.7186798366743337 6 0.6233807047356472 2.014274789647622 7 0.40777776987003334 1.537218836931484 8 0.29318857712897983 1.2631389989824322 9 0.21282263330610185 1.0315123527524563 >10 1.2184605773873782 12.313864245755617 >50 0.10694365408037299 4.013270412465357 >100 0.055703521917140675 5.204453202643963 >500 0.0021857071511680796 0.8201178917244362 >1k 0.0014254611855443991 1.8995516871847666 >5k 1.9006149140591988E-4 0.7171489558024662 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7316 0.37335178658730134 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6697 0.3417628369020171 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4649 0.23724883212744174 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4378 0.2234190980972123 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3559 0.18162370263316094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3168 0.1616701011356712 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3079 0.15712823276411986 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2878 0.14687075475645892 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2376 0.12125257585175342 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2212 0.11288329031316437 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1993 0.10170723218541437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.5516114446917808E-5 0.0 16-17 0.0 0.0 0.0 5.1032228893835616E-5 0.0 18-19 0.0 0.0 0.0 5.1032228893835616E-5 0.0 20-21 0.0 0.0 0.0 5.1032228893835616E-5 0.0 22-23 0.0 0.0 0.0 5.1032228893835616E-5 0.0 24-25 0.0 0.0 0.0 2.0412891557534246E-4 0.0 26-27 0.0 0.0 0.0 3.827417167037671E-4 0.0 28-29 0.0 0.0 0.0 9.951284634297946E-4 0.0 30-31 0.0 0.0 0.0 0.0025771275591386987 0.0 32-33 0.0 0.0 0.0 0.004924610088255137 0.0 34-35 0.0 0.0 0.0 0.007654834334075341 0.0 36-37 0.0 0.0 0.0 0.010257478007660959 0.0 38-39 0.0 0.0 0.0 0.012834605566799656 0.0 40-41 0.0 0.0 0.0 0.017376473938351025 0.0 42-43 0.0 0.0 0.0 0.02293898688777911 0.0 44-45 0.0 0.0 0.0 0.02862908040944178 0.0 46-47 0.0 0.0 0.0 0.03567152799679109 0.0 48-49 0.0 0.0 0.0 0.04279052392748116 0.0 50-51 0.0 0.0 0.0 0.054068646513018834 0.0 52-53 0.0 0.0 0.0 0.08279979138024829 0.0 54-55 0.0 0.0 0.0 0.12135464030954107 0.0 56-57 0.0 0.0 0.0 0.15756200670971746 0.0 58-59 0.0 0.0 0.0 0.2158152959920308 0.0 60-61 0.0 0.0 0.0 0.29491525077747605 0.0 62-63 0.0 0.0 0.0 0.35084657364511984 0.0 64-65 0.0 0.0 0.0 0.3973369341674041 0.0 66-67 0.0 0.0 0.0 0.519967380199291 0.0 68-69 0.0 0.0 0.0 0.6507119506252979 0.0 70-71 0.0 0.0 0.0 0.7167476548139212 0.0 72-73 2.5516114446917808E-5 0.0 0.0 0.8350658775042791 0.0 74-75 5.1032228893835616E-5 0.0 0.0 1.0152351616139657 0.0 76-77 5.1032228893835616E-5 0.0 0.0 1.2082645674048988 0.0 78-79 5.1032228893835616E-5 0.0 0.0 1.3914192369048748 0.0 80-81 5.1032228893835616E-5 0.0 0.0 1.6187933327413595 0.0 82-83 5.1032228893835616E-5 0.0 0.0 1.8690043510078356 0.0 84-85 5.1032228893835616E-5 0.0 0.0 2.127431558126219 0.0 86-87 5.1032228893835616E-5 0.0 0.0 2.465596622891221 0.0 88-89 5.1032228893835616E-5 0.0 0.0 2.8533905302554774 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9520 0.0 27.414677 1 TACATGG 10030 0.0 25.725132 2 ACATGGG 9795 0.0 24.644384 3 GAGTACT 6010 0.0 22.329292 12-13 CATGGGA 5985 0.0 21.514463 4 GTACCTG 1570 0.0 21.498413 1 GTAAGCG 235 0.0 21.220345 94-95 GTACTTT 6510 0.0 20.612185 14-15 AGTACTT 6585 0.0 20.018806 12-13 GTGTAGC 1045 0.0 20.016312 1 TACCTGG 1615 0.0 19.713375 2 GTATAGG 665 0.0 19.301443 1 AGAGTAC 11590 0.0 19.202473 10-11 GTATCAA 17310 0.0 19.141827 1 AGTACAT 7015 0.0 19.102058 2 CATGGGG 3850 0.0 18.388695 4 GAGTACA 8170 0.0 18.270702 1 ATGGGAG 2720 0.0 17.468088 5 TACTTTT 7510 0.0 17.329945 14-15 TAGCCCT 1225 0.0 17.066402 4 >>END_MODULE