FastQCFastQC Report
Thu 26 May 2016
SRR937239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959546
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84630.4318857531285308No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83890.428109368190387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65300.33324045467674657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44780.22852232098659586No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33210.16947803215642807No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33100.16891667763859589No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26300.13421476199078766No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24870.12691715325896916No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24420.12462070295874658No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22260.11359774151767808No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21000.10716768067705479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG91100.023.839911
GAGTACT78900.022.60974112-13
TACATGG94800.022.3961412
ACATGGG93350.021.8281523
GTACTTT83600.021.22117414-15
AGTACTT82750.021.09852212-13
CATGGGA60400.019.8169884
GTATAGG7950.019.7266621
AGAGTAC142150.019.11665210-11
TATAGCG1801.2343106E-418.471395
GTATCAA206600.018.1030271
ACTTTTT98550.017.92917616-17
GTATACG1600.001191782517.8116954
TACTTTT97000.017.7999114-15
CCGAGCG2002.7582294E-416.6276449
ACTCCGT2002.7625283E-416.624256
CTAGGAC13150.016.254023
CAACGCA230700.016.2032135
TCAACGC231000.016.1616084
ATCAACG231600.016.099233
Produced by FastQC (version 0.11.5)